Rw3G011420

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001859
Physical Location & Seq
Reverse (-)
11071042 .. 11073071
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011420.1

Sequence Viewer

Length: 948 bp
ATGGCGGACGATATTGGTTTCTTTGAGTTGAACACCGGAGCTAAGATGCCTTCTTTTGGCTTAGGGACCTGGCAGGCCAGCCCTGGCTTGGTCGGTGACGCCGTCGCTGCCGCCATCAAGGCAGGATATCGGCATATGGATTGTGCTCCAGTCTATGGCAATGAGAAGGAGATTGGTTTAGTTTTGAAGAAGCTGTTTGAAGACGGTGTGGTGAAGCGCGAGGAGTTGTGGATTACCTCCAAACTAGCGTCTACTGATCATGCACCAGAAGATGTACCAGATGCATTGGATAGGACTTTGAGAGACTTGCAGCTTGATTATGTTGACTTGTACCTGATCCACGGGCCAGTGCGGATGAAGAAGGATTCAGTAGGTTCTGGGCCTGAAAACCTTATACAACCAGATATCCCTGCCACATGGAGAGCAATGGAAACTCTCTTTGATTCGTGCAAGGCTCGAGCTATTGGTGTGAGCAATTTCGCTACAAAGAAGCTGTCAGATTTGCTGGATGTGGCACGAGTTCCTCCTGCTGTTAACCAAGTGGAGTGCCATCCTTCATGGCAGCAGGCCAAGCTACGATCCTTCTGCAAATCCAAGGGGGTTCACCTTTCTGGATATTCACCGCTGGGTTCTCCTGGCACAACATTTATCAAAGGCGATGTCCTTAAGAATCCGATTCTTCTCAGCGTGGCAGAGAAGCTAGGCAAGACTCCTGCACAGATTGCTCTTCGATGGGGACTGCAAATGGGTCATAGTGTTCTTCCAAAGAGTACTAATGAAGCAAGGATCAAGGAGAATCTTGATGTCTTTGGCTGGTCTATCCCAGAAGACTTGTTTGCCAAATTTTCTGAAATTGAGCAGGAAAGGCTATTCGATGGGACCATTTTTGTACATGACACTTTTGGACCCTATAGAAGTGTGGATGAACTCTGGGATGGTGAAATCTAG

Protein Analysis

315

Amino Acids

34.73

Weight (kDa)

5.45

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 20 - 285 7.1e-48 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 155
AccI GTMKAC 1 cut(s) 251
AccII CGCG 1 cut(s) 219
AciI CCGC 4 cut(s) 5, 111, 352, 623
AclWI GGATC 3 cut(s) 331, 573, 794
AcsI RAATTY 1 cut(s) 842
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 4 cut(s) 276, 332, 772, 891
AfiI CCNNNNNNNGG 3 cut(s) 56, 88, 155
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 3 cut(s) 31, 187, 200
AjnI CCWGG 3 cut(s) 68, 82, 634
AluBI AGCT 7 cut(s) 41, 193, 313, 461, 493, 574, 700
AluI AGCT 7 cut(s) 41, 193, 313, 461, 493, 574, 700
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 3 cut(s) 331, 573, 794
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 4 cut(s) 75, 344, 380, 567
ApeKI GCWGC 3 cut(s) 107, 310, 562
ApoI RAATTY 1 cut(s) 842
ArsI GACNNNNNNTTYG 4 cut(s) 479, 511, 645, 677
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 5 cut(s) 66, 344, 380, 879, 905
AsuHPI GGTGA 4 cut(s) 107, 223, 596, 612
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 3 cut(s) 66, 879, 905
BauI CACGAG 1 cut(s) 516
BbsI GAAGAC 2 cut(s) 207, 834
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 94, 322, 574
BccI CCATC 5 cut(s) 122, 558, 726, 869, 929
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 3 cut(s) 70, 84, 636
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 3 cut(s) 245, 701, 946
BfmI CTRYAG 1 cut(s) 910
BfrI CTTAAG 1 cut(s) 665
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 4 cut(s) 108, 111, 311, 563
BlsI GCNGC 4 cut(s) 109, 112, 312, 564
BmcAI AGTACT 1 cut(s) 772
Bme1390I CCNGG 3 cut(s) 70, 84, 636
Bme18I GGWCC 3 cut(s) 66, 879, 905
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 5 cut(s) 66, 344, 380, 879, 905
BmiI GGNNCC 3 cut(s) 67, 880, 907
BmrFI CCNGG 3 cut(s) 70, 84, 636
BmsI GCATC 2 cut(s) 36, 271
BpiI GAAGAC 2 cut(s) 207, 834
BpmI CTGGAG 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 61
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 82, 340, 594
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 88, 155
Bse1I ACTGG 2 cut(s) 149, 347
Bse3DI GCAATG 2 cut(s) 166, 432
BseBI CCWGG 3 cut(s) 70, 84, 636
BseDI CCNNGG 3 cut(s) 82, 340, 594
BseGI GGATG 5 cut(s) 360, 514, 550, 928, 940
BseLI CCNNNNNNNGG 3 cut(s) 56, 88, 155
BseMI GCAATG 2 cut(s) 166, 432
BseMII CTCAG 1 cut(s) 697
BseNI ACTGG 2 cut(s) 149, 347
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 94, 322, 574
BseYI CCCAGC 1 cut(s) 625
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 4 cut(s) 77, 346, 382, 569
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 3 cut(s) 79, 750, 892
BslI CCNNNNNNNGG 3 cut(s) 56, 88, 155
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 3 cut(s) 79, 750, 892
BsnI GGCC 4 cut(s) 77, 346, 382, 569
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 148
Bsp1407I TGTACA 1 cut(s) 889
Bsp143I GATC 4 cut(s) 256, 336, 578, 786
BspACI CCGC 4 cut(s) 5, 111, 352, 623
BspANI GGCC 4 cut(s) 77, 346, 382, 569
BspCNI CTCAG 1 cut(s) 696
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 67, 880, 907
BspPI GGATC 3 cut(s) 331, 573, 794
BspQI GCTCTTC 1 cut(s) 732
BspTI CTTAAG 1 cut(s) 665
BsrDI GCAATG 2 cut(s) 166, 432
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 2 cut(s) 149, 347
BssECI CCNNGG 3 cut(s) 82, 340, 594
BssMI GATC 4 cut(s) 256, 336, 578, 786
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 516
BssT1I CCWWGG 1 cut(s) 594
Bst2BI CACGAG 1 cut(s) 516
Bst2UI CCWGG 3 cut(s) 70, 84, 636
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 889
BstC8I GCNNGC 3 cut(s) 75, 79, 567
BstDEI CTNAG 3 cut(s) 42, 61, 683
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 5 cut(s) 360, 514, 550, 928, 940
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 4 cut(s) 259, 339, 581, 789
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 256, 336, 578, 786
BstMWI GCNNNNNNNGC 5 cut(s) 107, 119, 571, 722, 865
BstNI CCWGG 3 cut(s) 70, 84, 636
BstSCI CCNGG 3 cut(s) 68, 82, 634
BstSFI CTRYAG 1 cut(s) 910
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 94, 322, 574
BstV2I GAAGAC 2 cut(s) 207, 834
BsuRI GGCC 4 cut(s) 77, 346, 382, 569
BtgI CCRYGG 1 cut(s) 340
BtgZI GCGATG 1 cut(s) 672
BtsCI GGATG 5 cut(s) 360, 514, 550, 928, 940
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 3 cut(s) 75, 79, 567
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 5 cut(s) 66, 344, 380, 879, 905
CseI GACGC 2 cut(s) 107, 237
Csp6I GTAC 4 cut(s) 275, 331, 771, 890
CviAII CATG 4 cut(s) 260, 417, 558, 893
CviQI GTAC 4 cut(s) 275, 331, 771, 890
DdeI CTNAG 3 cut(s) 42, 61, 683
DpnI GATC 4 cut(s) 258, 338, 580, 788
DpnII GATC 4 cut(s) 256, 336, 578, 786
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 594
Eco32I GATATC 2 cut(s) 128, 406
Eco47I GGWCC 3 cut(s) 66, 879, 905
Eco88I CYCGRG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 68, 82, 634
EcoRV GATATC 2 cut(s) 128, 406
EcoT14I CCWWGG 1 cut(s) 594
EcoT22I ATGCAT 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 4 cut(s) 263, 420, 561, 896
FaqI GGGAC 3 cut(s) 79, 750, 892
FatI CATG 4 cut(s) 259, 416, 557, 892
FauNDI CATATG 1 cut(s) 135
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 4 cut(s) 108, 111, 311, 563
FokI GGATG 4 cut(s) 367, 521, 537, 935
Fsp4HI GCNGC 4 cut(s) 108, 111, 311, 563
FspBI CTAG 3 cut(s) 245, 701, 946
GlaI GCGC 1 cut(s) 218
GluI GCNGC 4 cut(s) 108, 111, 311, 563
GsaI CCCAGC 1 cut(s) 629
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 4 cut(s) 77, 346, 382, 569
HapII CCGG 1 cut(s) 36
HgaI GACGC 2 cut(s) 107, 237
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 4 cut(s) 263, 420, 561, 896
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 2 cut(s) 325, 535
HindII GTYRAC 2 cut(s) 325, 535
HinfI GANTC 6 cut(s) 365, 443, 670, 676, 709, 796
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 1 cut(s) 36
HphI GGTGA 4 cut(s) 107, 223, 596, 612
Hpy166II GTNNAC 4 cut(s) 252, 325, 535, 604
Hpy188I TCNGA 3 cut(s) 499, 675, 850
Hpy188III TCNNGA 2 cut(s) 612, 800
Hpy8I GTNNAC 4 cut(s) 252, 325, 535, 604
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 5 cut(s) 60, 160, 355, 564, 592
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4V TGCA 7 cut(s) 263, 284, 310, 450, 588, 716, 742
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 119, 571, 722, 865
HpyF3I CTNAG 3 cut(s) 42, 61, 683
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 4 cut(s) 263, 420, 561, 896
HspAI GCGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 256
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 4 cut(s) 256, 336, 578, 786
LguI GCTCTTC 1 cut(s) 732
LmnI GCTCC 2 cut(s) 38, 151
Lsp1109I GCAGC 3 cut(s) 94, 322, 574
LweI GCATC 2 cut(s) 36, 271
MaeI CTAG 3 cut(s) 245, 701, 946
MaeIII GTNAC 1 cut(s) 95
MalI GATC 4 cut(s) 258, 338, 580, 788
MboI GATC 4 cut(s) 256, 336, 578, 786
MboII GAAGA 8 cut(s) 199, 212, 281, 370, 671, 719, 752, 839
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 3 cut(s) 475, 842, 852
MlyI GAGTC 1 cut(s) 703
MnlI CCTC 3 cut(s) 214, 247, 534
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 2 cut(s) 534, 666
MspA1I CMGCKG 1 cut(s) 625
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 70, 84, 636
MvaI CCWGG 3 cut(s) 70, 84, 636
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 5 cut(s) 107, 119, 571, 722, 865
NdeI CATATG 1 cut(s) 135
NdeII GATC 4 cut(s) 256, 336, 578, 786
NlaIII CATG 4 cut(s) 263, 420, 561, 896
NlaIV GGNNCC 3 cut(s) 67, 880, 907
NmuCI GTSAC 1 cut(s) 95
NsiI ATGCAT 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 732
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 5 cut(s) 365, 443, 670, 676, 796
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 4 cut(s) 109, 112, 312, 564
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
PpuMI RGGWCCY 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 68, 82, 634
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 3 cut(s) 68, 82, 634
PspN4I GGNNCC 3 cut(s) 67, 880, 907
PspPI GGNCC 5 cut(s) 66, 344, 380, 879, 905
PspPPI RGGWCCY 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 4 cut(s) 276, 332, 772, 891
RsaNI GTAC 4 cut(s) 275, 331, 771, 890
SapI GCTCTTC 1 cut(s) 732
SaqAI TTAA 2 cut(s) 534, 666
SatI GCNGC 4 cut(s) 108, 111, 311, 563
Sau3AI GATC 4 cut(s) 256, 336, 578, 786
Sau96I GGNCC 5 cut(s) 66, 344, 380, 879, 905
ScaI AGTACT 1 cut(s) 772
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 3 cut(s) 70, 84, 636
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 2 cut(s) 36, 271
SfcI CTRYAG 1 cut(s) 910
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 3 cut(s) 66, 879, 905
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 456, 665
SmoI CTYRAG 2 cut(s) 456, 665
Sse9I AATT 3 cut(s) 475, 842, 852
SsiI CCGC 4 cut(s) 5, 111, 352, 623
SspMI CTAG 3 cut(s) 245, 701, 946
StyD4I CCNGG 3 cut(s) 68, 82, 634
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 1 cut(s) 206
TaqI TCGA 3 cut(s) 457, 730, 873
TasI AATT 3 cut(s) 475, 842, 852
TatI WGTACW 2 cut(s) 770, 889
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 5 cut(s) 365, 443, 670, 676, 796
Tru1I TTAA 2 cut(s) 534, 666
Tru9I TTAA 2 cut(s) 534, 666
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 107, 310, 562
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 371, 546, 792, 939
TspRI CASTG 1 cut(s) 354
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 3 cut(s) 66, 879, 905
XapI RAATTY 1 cut(s) 842
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 3 cut(s) 245, 701, 946
ZrmI AGTACT 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.