Rh3BG140500

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001859
Physical Location & Seq
Reverse (-)
11918179 .. 11920267
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG140500.1

Sequence Viewer

Length: 897 bp
ATGGCGGACGATATTGGTTTCTTTGAGTTGAACACCGGAGCCAAGATGCCTTCTTTGGGATTAGGGACCTGGCAGTCCAGCCCCGGCTTGGTCGGTGAAGCCGTCGCCACCGCCATCAAGATTGGTTTGGTTTTGAAGAAGCTGTTTGAAGATGGTGTGGTGAAGCGCGAAGAATTGTGGATTACCTCCAAGCTCTGGTGTACGGATCATGCCCCAGAAGATGTACCAGAGGCATTGGATAGGACTTTGAAAGACTTGCAGCTTGATTATGTTGACTTGTACTTGATCCACTGGCCAGTGCGGATGAAGAAGGGTGCAGTCGGTTTTAAGCCTGAAAACCTTGTCGAACCAGATATCCCTGGTACTTGGAGAGCAATGGAAACTCTCTATGATTCGTGCAAGGCTCGAGCTATTGGTCTGAGCAATTTCGCTACAAAGAAGCTGTCAGATTTGCTGGATGTGGCACGTGTCCCTCCTGCTGTTGACCAAGTGGAGTGCCATCCTTCATGGCAGCAGACCAAGCTGCGATCCTTCTGCAAATCCAAGGGGGTTCACCTTTCTGGATATTCACCACTGGGTTCTCCTGGCACAACATGGATCAAAAGTGATGTCCTTAAGAATCCGATTCTTCTCAGCGTTGCAGAGAAGCTAGGCAAAACTCCTGCTCAAGTTGCTCTTCGATGGGGACTGCAAATGGGTCATAGCGTTGTTCCAAAGAGTACTAATGAAGCAAGGATCAAGGAGAATCTCGATGTCTTTGGCTGGTCTATCCCAGAGGACTTGTTTGCCAAGTTTTCTGAAATTGAGCAGGCAAGGCTGATTAGAGGGAACTCATTTGTTCATGACACTTTTGGACCCTATAGAAGTGTTGAAGAACTATGGGATGGTGAAATCTAG

Protein Analysis

298

Amino Acids

33.16

Weight (kDa)

5.93

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 42 - 268 4.4e-39 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
AccB7I CCANNNNNTGG 1 cut(s) 195
AccII CGCG 1 cut(s) 168
AciI CCGC 3 cut(s) 5, 111, 301
AclWI GGATC 5 cut(s) 213, 280, 522, 605, 743
AcoI YGGCCR 1 cut(s) 293
AcvI CACGTG 1 cut(s) 467
AfaI GTAC 5 cut(s) 202, 225, 281, 364, 721
AfiI CCNNNNNNNGG 3 cut(s) 56, 88, 195
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 5 cut(s) 31, 136, 149, 250, 872
AjnI CCWGG 3 cut(s) 68, 358, 583
AloI GAACNNNNNNTCC 2 cut(s) 339, 371
AluBI AGCT 7 cut(s) 142, 193, 262, 410, 442, 523, 649
AluI AGCT 7 cut(s) 142, 193, 262, 410, 442, 523, 649
AlwI GGATC 5 cut(s) 213, 280, 522, 605, 743
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 3 cut(s) 259, 511, 523
ArsI GACNNNNNNTTYG 4 cut(s) 428, 460, 594, 626
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 66, 854
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 107, 172, 545, 561
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 2 cut(s) 66, 854
BalI TGGCCA 1 cut(s) 295
BbrPI CACGTG 1 cut(s) 467
BbvI GCAGC 3 cut(s) 271, 510, 523
BccI CCATC 5 cut(s) 122, 146, 507, 675, 878
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 516, 550
BciT130I CCWGG 3 cut(s) 70, 360, 585
BcnI CCSGG 1 cut(s) 84
BfaI CTAG 2 cut(s) 650, 895
BfmI CTRYAG 1 cut(s) 859
BfrI CTTAAG 1 cut(s) 614
BisI GCNGC 3 cut(s) 260, 512, 524
BlsI GCNGC 3 cut(s) 261, 513, 525
BmcAI AGTACT 1 cut(s) 721
Bme1390I CCNGG 4 cut(s) 70, 84, 360, 585
Bme18I GGWCC 2 cut(s) 66, 854
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 2 cut(s) 66, 854
BmiI GGNNCC 3 cut(s) 40, 67, 856
BmrFI CCNGG 4 cut(s) 70, 84, 360, 585
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 584
BpuEI CTTGAG 1 cut(s) 651
BpuMI CCSGG 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 82, 358, 543
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 88, 195
Bse1I ACTGG 3 cut(s) 296, 296, 579
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 3 cut(s) 70, 360, 585
BseDI CCNNGG 3 cut(s) 82, 358, 543
BseGI GGATG 4 cut(s) 309, 463, 499, 889
BseLI CCNNNNNNNGG 3 cut(s) 56, 88, 195
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 410, 646
BseNI ACTGG 3 cut(s) 296, 296, 579
BseXI GCAGC 3 cut(s) 271, 510, 523
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 2 cut(s) 36, 84
BslFI GGGAC 3 cut(s) 79, 455, 699
BslI CCNNNNNNNGG 3 cut(s) 56, 88, 195
BsmFI GGGAC 3 cut(s) 79, 455, 699
BsnI GGCC 1 cut(s) 295
BsoBI CYCGRG 1 cut(s) 405
Bsp143I GATC 5 cut(s) 205, 285, 527, 597, 735
BspACI CCGC 3 cut(s) 5, 111, 301
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 2 cut(s) 411, 645
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 841
BspLI GGNNCC 3 cut(s) 40, 67, 856
BspPI GGATC 5 cut(s) 213, 280, 522, 605, 743
BspQI GCTCTTC 1 cut(s) 681
BspTI CTTAAG 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 3 cut(s) 296, 296, 579
BssECI CCNNGG 3 cut(s) 82, 358, 543
BssMI GATC 5 cut(s) 205, 285, 527, 597, 735
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 3 cut(s) 70, 360, 585
Bst6I CTCTTC 1 cut(s) 681
BstAFI CTTAAG 1 cut(s) 614
BstBAI YACGTR 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 810
BstDEI CTNAG 2 cut(s) 419, 632
BstF5I GGATG 4 cut(s) 309, 463, 499, 889
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 5 cut(s) 208, 288, 530, 600, 738
BstMBI GATC 5 cut(s) 205, 285, 527, 597, 735
BstMWI GCNNNNNNNGC 3 cut(s) 520, 671, 814
BstNI CCWGG 3 cut(s) 70, 360, 585
BstSCI CCNGG 4 cut(s) 68, 82, 358, 583
BstSFI CTRYAG 1 cut(s) 859
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 271, 510, 523
BsuRI GGCC 1 cut(s) 295
BtsCI GGATG 4 cut(s) 309, 463, 499, 889
BtsIMutI CAGTG 3 cut(s) 289, 303, 572
Cac8I GCNNGC 1 cut(s) 810
CciI TCATGA 1 cut(s) 841
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 66, 854
Csp6I GTAC 5 cut(s) 201, 224, 280, 363, 720
CviAII CATG 4 cut(s) 209, 507, 594, 842
CviQI GTAC 5 cut(s) 201, 224, 280, 363, 720
DdeI CTNAG 2 cut(s) 419, 632
DpnI GATC 5 cut(s) 207, 287, 529, 599, 737
DpnII GATC 5 cut(s) 205, 285, 527, 597, 735
DrdI GACNNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 543
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 66, 854
Eco72I CACGTG 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 68, 358, 583
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 4 cut(s) 212, 510, 597, 845
FaiI YATR 9 cut(s) 210, 270, 390, 508, 595, 702, 843, 861, 880
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 3 cut(s) 79, 455, 699
FatI CATG 4 cut(s) 208, 506, 593, 841
Fnu4HI GCNGC 3 cut(s) 260, 512, 524
FokI GGATG 3 cut(s) 316, 470, 486
Fsp4HI GCNGC 3 cut(s) 260, 512, 524
FspBI CTAG 2 cut(s) 650, 895
GlaI GCGC 1 cut(s) 167
GluI GCNGC 3 cut(s) 260, 512, 524
HaeIII GGCC 1 cut(s) 295
HapII CCGG 2 cut(s) 36, 84
HhaI GCGC 1 cut(s) 168
Hin1II CATG 4 cut(s) 212, 510, 597, 845
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 2 cut(s) 274, 484
HindII GTYRAC 2 cut(s) 274, 484
HinfI GANTC 4 cut(s) 392, 619, 625, 745
HpaII CCGG 2 cut(s) 36, 84
HphI GGTGA 4 cut(s) 107, 172, 545, 561
Hpy166II GTNNAC 4 cut(s) 201, 274, 484, 553
Hpy188I TCNGA 4 cut(s) 420, 448, 624, 799
Hpy188III TCNNGA 4 cut(s) 118, 561, 749, 842
Hpy8I GTNNAC 4 cut(s) 201, 274, 484, 553
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 60, 304, 513, 541
HpyCH4IV ACGT 1 cut(s) 466
HpyCH4V TGCA 6 cut(s) 259, 317, 399, 537, 641, 691
HpyF10VI GCNNNNNNNGC 3 cut(s) 520, 671, 814
HpyF3I CTNAG 2 cut(s) 419, 632
HpySE526I ACGT 1 cut(s) 466
Hsp92II CATG 4 cut(s) 212, 510, 597, 845
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 5 cut(s) 205, 285, 527, 597, 735
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 3 cut(s) 271, 510, 523
LweI GCATC 1 cut(s) 36
MaeI CTAG 2 cut(s) 650, 895
MaeII ACGT 1 cut(s) 466
MalI GATC 5 cut(s) 207, 287, 529, 599, 737
MboI GATC 5 cut(s) 205, 285, 527, 597, 735
MboII GAAGA 8 cut(s) 148, 161, 182, 230, 319, 620, 668, 884
MlsI TGGCCA 1 cut(s) 295
MluCI AATT 3 cut(s) 173, 424, 801
MluNI TGGCCA 1 cut(s) 295
MnlI CCTC 5 cut(s) 196, 223, 483, 769, 818
Mox20I TGGCCA 1 cut(s) 295
MscI TGGCCA 1 cut(s) 295
MseI TTAA 2 cut(s) 327, 615
Msp20I TGGCCA 1 cut(s) 295
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 2 cut(s) 36, 84
MspR9I CCNGG 4 cut(s) 70, 84, 360, 585
MvaI CCWGG 3 cut(s) 70, 360, 585
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 3 cut(s) 520, 671, 814
NciI CCSGG 1 cut(s) 84
NdeII GATC 5 cut(s) 205, 285, 527, 597, 735
NlaIII CATG 4 cut(s) 212, 510, 597, 845
NlaIV GGNNCC 3 cut(s) 40, 67, 856
PaeR7I CTCGAG 1 cut(s) 405
PagI TCATGA 1 cut(s) 841
PciSI GCTCTTC 1 cut(s) 681
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 4 cut(s) 392, 619, 625, 745
PflMI CCANNNNNTGG 1 cut(s) 195
PkrI GCNGC 3 cut(s) 261, 513, 525
PmaCI CACGTG 1 cut(s) 467
PmlI CACGTG 1 cut(s) 467
Ppu21I YACGTR 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 68, 358, 583
PspCI CACGTG 1 cut(s) 467
PspGI CCWGG 3 cut(s) 68, 358, 583
PspN4I GGNNCC 3 cut(s) 40, 67, 856
PspPI GGNCC 2 cut(s) 66, 854
PspPPI RGGWCCY 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 405
RsaI GTAC 5 cut(s) 202, 225, 281, 364, 721
RsaNI GTAC 5 cut(s) 201, 224, 280, 363, 720
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 2 cut(s) 327, 615
SatI GCNGC 3 cut(s) 260, 512, 524
Sau3AI GATC 5 cut(s) 205, 285, 527, 597, 735
Sau96I GGNCC 2 cut(s) 66, 854
ScaI AGTACT 1 cut(s) 721
ScrFI CCNGG 4 cut(s) 70, 84, 360, 585
SfaNI GCATC 1 cut(s) 36
SfcI CTRYAG 1 cut(s) 859
Sfr274I CTCGAG 1 cut(s) 405
SinI GGWCC 2 cut(s) 66, 854
SlaI CTCGAG 1 cut(s) 405
SmlI CTYRAG 3 cut(s) 405, 614, 666
SmoI CTYRAG 3 cut(s) 405, 614, 666
Sse9I AATT 3 cut(s) 173, 424, 801
SsiI CCGC 3 cut(s) 5, 111, 301
SspMI CTAG 2 cut(s) 650, 895
StyD4I CCNGG 4 cut(s) 68, 82, 358, 583
StyI CCWWGG 1 cut(s) 543
TaiI ACGT 1 cut(s) 469
TaqI TCGA 4 cut(s) 345, 406, 679, 750
TasI AATT 3 cut(s) 173, 424, 801
TatI WGTACW 2 cut(s) 279, 719
TfiI GAWTC 4 cut(s) 392, 619, 625, 745
Tru1I TTAA 2 cut(s) 327, 615
Tru9I TTAA 2 cut(s) 327, 615
TscAI CASTG 3 cut(s) 296, 303, 579
TseI GCWGC 3 cut(s) 259, 511, 523
TspDTI ATGAA 4 cut(s) 320, 495, 741, 830
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 3 cut(s) 296, 303, 579
Van91I CCANNNNNTGG 1 cut(s) 195
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 66, 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 405
XspI CTAG 2 cut(s) 650, 895
ZrmI AGTACT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.