FvH4_7g01562

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003476
Physical Location & Seq
Reverse (-)
1966409 .. 1966911
503 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01562.t1

Sequence Viewer

Length: 342 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTGGTCAAGCTCTTGTTGCAACGTGGTTATACTGTCAAAGCCACTGTCCGTGACCCAAATGGTGCAAAGAAAACAGAACACTTGCTTTCACTTGATGGAGCAAAGAAAAGGCTTCATTTGTTTAAAGCAGACTTGTTAGAAGAAGGTTCTTTTGATTCTGTAGTTGACGGATGTGAATGCATTTTCCATACGGCATCCCCTGTACTTCTTTCTGTCACTAACCCTCAGGTATCATCTCTGTCTTTGTTAACATACTCGGCACGTTTACCAGTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

114

Amino Acids

12.17

Weight (kDa)

8.4

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 86 1e-08 NmrA-like family
Epimerase PF01370 9 - 88 1.4e-10 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 11 - 93 3.3e-10 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 11 - 89 7.2e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 13 - 87 5.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 9
AfaI GTAC 1 cut(s) 267
AluBI AGCT 2 cut(s) 6, 73
AluI AGCT 2 cut(s) 6, 73
ApeKI GCWGC 1 cut(s) 6
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
AxyI CCTNAGG 1 cut(s) 288
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
BmsI GCATC 3 cut(s) 50, 65, 266
Bse1I ACTGG 1 cut(s) 332
Bse21I CCTNAGG 1 cut(s) 288
BseGI GGATG 2 cut(s) 239, 257
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 332
BsmI GAATGC 1 cut(s) 245
BspACI CCGC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 301
BsrI ACTGG 1 cut(s) 332
Bst4CI ACNGT 2 cut(s) 97, 109
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 239, 257
BstMWI GCNNNNNNNGC 3 cut(s) 15, 70, 79
BstSFI CTRYAG 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 239, 257
BtsIMutI CAGTG 1 cut(s) 105
Csp6I GTAC 1 cut(s) 266
CviAII CATG 1 cut(s) 60
CviJI RGCY 5 cut(s) 6, 64, 73, 104, 175
CviKI_1 RGCY 5 cut(s) 6, 64, 73, 104, 175
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 1 cut(s) 288
DraI TTTAAA 1 cut(s) 187
Eco81I CCTNAGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 245
FaeI CATG 1 cut(s) 63
FaiI YATR 5 cut(s) 53, 61, 93, 252, 316
FatI CATG 1 cut(s) 59
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 2 cut(s) 244, 246
Fsp4HI GCNGC 1 cut(s) 7
GluI GCNGC 1 cut(s) 7
Hin1II CATG 1 cut(s) 63
HincII GTYRAC 2 cut(s) 229, 312
HindII GTYRAC 2 cut(s) 229, 312
HinfI GANTC 1 cut(s) 218
HpaI GTTAAC 1 cut(s) 312
Hpy166II GTNNAC 3 cut(s) 229, 312, 329
Hpy8I GTNNAC 3 cut(s) 229, 312, 329
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 2 cut(s) 97, 109
HpyCH4IV ACGT 2 cut(s) 85, 325
HpyCH4V TGCA 3 cut(s) 82, 128, 243
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 70, 79
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 85, 325
Hsp92II CATG 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 5 cut(s) 21, 30, 50, 275, 276
LweI GCATC 3 cut(s) 50, 65, 266
MaeII ACGT 2 cut(s) 85, 325
MaeIII GTNAC 3 cut(s) 31, 113, 277
MboII GAAGA 1 cut(s) 215
MnlI CCTC 1 cut(s) 297
Mph1103I ATGCAT 1 cut(s) 245
MseI TTAA 2 cut(s) 186, 311
Mva1269I GAATGC 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 15, 70, 79
NlaIII CATG 1 cut(s) 63
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 3 cut(s) 31, 113, 277
NsiI ATGCAT 1 cut(s) 245
PctI GAATGC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 1 cut(s) 8
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SaqAI TTAA 2 cut(s) 186, 311
SatI GCNGC 1 cut(s) 7
SetI ASST 7 cut(s) 8, 24, 75, 88, 211, 294, 328
SfaNI GCATC 3 cut(s) 50, 65, 266
SfcI CTRYAG 1 cut(s) 222
SsiI CCGC 1 cut(s) 9
TaaI ACNGT 2 cut(s) 97, 109
TaiI ACGT 2 cut(s) 88, 328
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 2 cut(s) 186, 311
Tru9I TTAA 2 cut(s) 186, 311
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 3 cut(s) 31, 113, 277
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 3 cut(s) 31, 113, 277
TspDTI ATGAA 2 cut(s) 40, 167
TspGWI ACGGA 2 cut(s) 101, 246
TspRI CASTG 1 cut(s) 112
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.