RchiOBHm_Chr1g0317671

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003476
Physical Location & Seq
Reverse (-)
5215070 .. 5216016
947 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54796

Sequence Viewer

Length: 501 bp
ATGAACTGCGGAGAAAGCAAGGTTGTGTGTGTGACGGGAGCATCTGGTTACATAGCATCATGGCTGGTGAAGCTCTTGTTGCAACGAGGTTATACTGTCAAAGCCACGGTTCGGGACCCAGACGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTGATACGGCAATTCATGGATGTGAATGTCTTTTCCATACGGCGTCCCCTGTCCTACTCTCAGTCTCATCAACTAATCCGCAGGCAGAATTAATTGACCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATAGCAGCAGTTGGATATAATGGAAAACCTCTTACTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCATTTTGTGAAAAAACGAAGGTTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

166

Amino Acids

17.88

Weight (kDa)

6.07

Isoelectric Point (pI)

20.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 105 8.2e-10 NmrA-like family
Epimerase PF01370 9 - 141 1e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 140 8.9e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 131 8.9e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 139 1.4e-06 Male sterility protein
NAD_binding_10 PF13460 13 - 134 5.2e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AciI CCGC 2 cut(s) 9, 293
AclWI GGATC 2 cut(s) 352, 458
AcyI GRCGYC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 187, 322
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 2 cut(s) 352, 458
AlwNI CAGNNNCTG 1 cut(s) 467
ApeKI GCWGC 1 cut(s) 398
AseI ATTAAT 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 410
BccI CCATC 1 cut(s) 152
BceAI ACGGC 2 cut(s) 237, 270
BcoDI GTCTC 1 cut(s) 283
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 2 cut(s) 116, 117
BmsI GCATC 2 cut(s) 50, 65
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 105, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 105, 340
BseGI GGATG 2 cut(s) 239, 386
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 444
BslFI GGGAC 2 cut(s) 128, 244
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 283
BsmFI GGGAC 2 cut(s) 128, 244
Bsp143I GATC 3 cut(s) 124, 344, 463
BspACI CCGC 2 cut(s) 9, 293
BspCNI CTCAG 1 cut(s) 287
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 116, 117
BspPI GGATC 2 cut(s) 352, 458
BssECI CCNNGG 2 cut(s) 105, 340
BssMI GATC 3 cut(s) 124, 344, 463
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 340
Bst4CI ACNGT 2 cut(s) 97, 109
BstACI GRCGYC 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 1 cut(s) 274
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 2 cut(s) 239, 386
BstKTI GATC 3 cut(s) 127, 347, 466
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 3 cut(s) 124, 344, 463
BstMWI GCNNNNNNNGC 3 cut(s) 15, 70, 79
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
Bsu15I ATCGAT 1 cut(s) 444
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 2 cut(s) 239, 386
Cac8I GCNNGC 1 cut(s) 297
CaiI CAGNNNCTG 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 1 cut(s) 246
CviAII CATG 2 cut(s) 60, 230
CviJI RGCY 4 cut(s) 64, 73, 104, 175
CviKI_1 RGCY 4 cut(s) 64, 73, 104, 175
DdeI CTNAG 1 cut(s) 274
DpnI GATC 3 cut(s) 126, 346, 465
DpnII GATC 3 cut(s) 124, 344, 463
Eco130I CCWWGG 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 2 cut(s) 63, 233
FaiI YATR 8 cut(s) 53, 61, 93, 231, 252, 383, 395, 411
FaqI GGGAC 2 cut(s) 128, 244
FatI CATG 2 cut(s) 59, 229
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 2 cut(s) 246, 373
Fsp4HI GCNGC 1 cut(s) 399
GluI GCNGC 1 cut(s) 399
HgaI GACGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 2 cut(s) 63, 233
HinfI GANTC 1 cut(s) 449
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 200, 316, 349, 481
HpyCH4III ACNGT 2 cut(s) 97, 109
HpyCH4V TGCA 2 cut(s) 82, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 70, 79
HpyF3I CTNAG 1 cut(s) 274
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 2 cut(s) 63, 233
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 3 cut(s) 124, 344, 463
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 7 cut(s) 30, 50, 132, 276, 281, 327, 480
Lsp1109I GCAGC 1 cut(s) 410
LweI GCATC 2 cut(s) 50, 65
MaeIII GTNAC 2 cut(s) 31, 47
MalI GATC 3 cut(s) 126, 346, 465
MboI GATC 3 cut(s) 124, 344, 463
MboII GAAGA 1 cut(s) 215
MflI RGATCY 1 cut(s) 463
MluCI AATT 3 cut(s) 225, 302, 306
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 3 cut(s) 80, 400, 432
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 2 cut(s) 305, 333
MslI CAYNNNNRTG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 15, 70, 79
NdeII GATC 3 cut(s) 124, 344, 463
NlaIII CATG 2 cut(s) 63, 233
NlaIV GGNNCC 2 cut(s) 116, 117
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 1 cut(s) 400
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 305
PsiI TTATAA 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 1 cut(s) 115
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 2 cut(s) 305, 333
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 3 cut(s) 124, 344, 463
Sau96I GGNCC 1 cut(s) 115
SetI ASST 7 cut(s) 24, 75, 91, 147, 360, 424, 492
SfaNI GCATC 2 cut(s) 50, 65
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 234
Sse9I AATT 3 cut(s) 225, 302, 306
SsiI CCGC 2 cut(s) 9, 293
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 97, 109
TaqI TCGA 1 cut(s) 444
TasI AATT 3 cut(s) 225, 302, 306
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 305, 333
Tru9I TTAA 2 cut(s) 305, 333
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 17, 167, 218, 462
VpaK11BI GGWCC 1 cut(s) 115
VspI ATTAAT 1 cut(s) 305
XmnI GAANNNNTTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.