Rh1AG028600

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003476
Physical Location & Seq
Reverse (-)
5168980 .. 5170871
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG028600.1

Sequence Viewer

Length: 498 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGATTCATAGCATCATGGCTGGTGAAGCTTTTATTGCAAAGAGGTTTTACTGTCAAAGCCACTGTTCGTGACCCAAATGATCAAAAGAAAACAGATCACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGTTCTTTTGATTCTGTAATTGATGGATGCGAATGTGTTTTCCATGTGGCATCCCCTGTAATACTTTCAGACCTCACTGACCCCCAGGCAGAATTACTTGACCCTGCGTTGAAGGGAACACTTAATGTCCTTGGATCCTGTGTGAAGGTTCCGTCTACCAAAAGGGTGGTTATAACATCCTCCATGGCAGCAGTTGCATTTACTGGAAAACCTCTTGCTGCTGATGTAATAATCGACGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAGAAACAAAGGTTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

165

Amino Acids

17.67

Weight (kDa)

5.0

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 106 6.2e-10 NmrA-like family
Epimerase PF01370 9 - 133 2.2e-13 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 136 1.8e-14 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 130 5.4e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 135 3.5e-08 Male sterility protein
NAD_binding_10 PF13460 13 - 134 2.6e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AccI GTMKAC 1 cut(s) 362
AciI CCGC 1 cut(s) 9
AclWI GGATC 3 cut(s) 336, 349, 455
AgsI TTSAA 2 cut(s) 187, 319
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 2 cut(s) 6, 73
AluI AGCT 2 cut(s) 6, 73
AlwI GGATC 3 cut(s) 336, 349, 455
AlwNI CAGNNNCTG 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 6, 395, 425
Asp700I GAANNNNTTC 1 cut(s) 174
AsuHPI GGTGA 2 cut(s) 79, 134
BamHI GGATCC 1 cut(s) 341
BbvI GCAGC 2 cut(s) 407, 412
BccI CCATC 2 cut(s) 152, 224
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 1 cut(s) 124
BisI GCNGC 3 cut(s) 7, 396, 426
BlsI GCNGC 3 cut(s) 8, 397, 427
Bme1390I CCNGG 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 343, 357
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 4 cut(s) 50, 65, 224, 266
BsaJI CCNNGG 3 cut(s) 291, 337, 390
Bse1I ACTGG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 291, 337, 390
BseGI GGATG 3 cut(s) 239, 257, 383
BseNI ACTGG 1 cut(s) 415
BseXI GCAGC 2 cut(s) 407, 412
Bsp143I GATC 4 cut(s) 124, 139, 341, 460
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 343, 357
BspPI GGATC 3 cut(s) 336, 349, 455
BsrI ACTGG 1 cut(s) 415
BssECI CCNNGG 3 cut(s) 291, 337, 390
BssMI GATC 4 cut(s) 124, 139, 341, 460
BssT1I CCWWGG 2 cut(s) 337, 390
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 97, 109
BstAPI GCANNNNNTGC 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 3 cut(s) 239, 257, 383
BstKTI GATC 4 cut(s) 127, 142, 344, 463
BstMBI GATC 4 cut(s) 124, 139, 341, 460
BstMWI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 401
BstNI CCWGG 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 407, 412
BstX2I RGATCY 2 cut(s) 341, 460
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 2 cut(s) 341, 460
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 239, 257, 383
BtsIMutI CAGTG 2 cut(s) 105, 282
CaiI CAGNNNCTG 1 cut(s) 464
CviAII CATG 3 cut(s) 60, 251, 391
CviJI RGCY 5 cut(s) 6, 64, 73, 104, 175
CviKI_1 RGCY 5 cut(s) 6, 64, 73, 104, 175
DpnI GATC 4 cut(s) 126, 141, 343, 462
DpnII GATC 4 cut(s) 124, 139, 341, 460
Eco130I CCWWGG 2 cut(s) 337, 390
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 2 cut(s) 337, 390
ErhI CCWWGG 2 cut(s) 337, 390
FaeI CATG 3 cut(s) 63, 254, 394
FaiI YATR 5 cut(s) 53, 61, 252, 380, 392
FatI CATG 3 cut(s) 59, 250, 390
FbaI TGATCA 1 cut(s) 124
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 3 cut(s) 7, 396, 426
FokI GGATG 3 cut(s) 244, 246, 370
Fsp4HI GCNGC 3 cut(s) 7, 396, 426
GluI GCNGC 3 cut(s) 7, 396, 426
Hin1II CATG 3 cut(s) 63, 254, 394
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 48, 218, 446
HphI GGTGA 2 cut(s) 79, 134
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 2 cut(s) 277, 460
Hpy188III TCNNGA 2 cut(s) 45, 113
Hpy8I GTNNAC 1 cut(s) 363
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 3 cut(s) 200, 313, 346
HpyCH4III ACNGT 2 cut(s) 97, 109
HpyCH4V TGCA 2 cut(s) 82, 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 401
Hsp92II CATG 3 cut(s) 63, 254, 394
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 4 cut(s) 124, 139, 341, 460
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 2 cut(s) 407, 412
LweI GCATC 4 cut(s) 50, 65, 224, 266
MaeIII GTNAC 2 cut(s) 31, 113
MalI GATC 4 cut(s) 126, 141, 343, 462
MboI GATC 4 cut(s) 124, 139, 341, 460
MboII GAAGA 2 cut(s) 215, 488
MflI RGATCY 2 cut(s) 341, 460
MluCI AATT 2 cut(s) 225, 299
MnlI CCTC 4 cut(s) 80, 290, 397, 429
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 401
NcoI CCATGG 1 cut(s) 390
NdeII GATC 4 cut(s) 124, 139, 341, 460
NlaIII CATG 3 cut(s) 63, 254, 394
NlaIV GGNNCC 2 cut(s) 343, 357
NmuCI GTSAC 2 cut(s) 31, 113
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 3 cut(s) 48, 218, 446
PkrI GCNGC 3 cut(s) 8, 397, 427
PsiI TTATAA 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 343, 357
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 341, 460
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 3 cut(s) 7, 396, 426
Sau3AI GATC 4 cut(s) 124, 139, 341, 460
ScrFI CCNGG 1 cut(s) 293
SfaNI GCATC 4 cut(s) 50, 65, 224, 266
Sse9I AATT 2 cut(s) 225, 299
SsiI CCGC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 2 cut(s) 337, 390
TaaI ACNGT 2 cut(s) 97, 109
TaqI TCGA 1 cut(s) 441
TasI AATT 2 cut(s) 225, 299
TfiI GAWTC 3 cut(s) 48, 218, 446
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 112, 289
TseFI GTSAC 2 cut(s) 31, 113
TseI GCWGC 3 cut(s) 6, 395, 425
Tsp45I GTSAC 2 cut(s) 31, 113
TspDTI ATGAA 2 cut(s) 40, 167
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 2 cut(s) 112, 289
XmiI GTMKAC 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.