Rh1BG023500

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003476
Physical Location & Seq
Reverse (-)
3124015 .. 3128063
4049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG023500.1

Sequence Viewer

Length: 531 bp
ATGGGCCGAGAGAGGAAGGTTGTGTGTGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGCTGGTGAAGATTCTGTTACAGAGAGGTTATGTCGTCAAAGCCACCGTTCGTGACCCAAATGATCCAAAGAAAACAGAACACTTGCTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGTAGTTGATGGATGTGAAGGTGTTTTCCATACAGCATCTCCGGTACTATTTTCATCAATCAGCGACCCACAGGCAGAACTGATTGATCCTGCAGTAAAGGGAACGCTTAATGTTCTTAAATCGTGCGTGAAATTTCCAACAATCAAGAGGGTAGTTTTAACATCTTCCATGGCTGCAGTTATTATCAATGGAAGACCTTTAACCTCTAATGTAGAAGTTGATGAAACATGGTTTTCTGATCCACTTGTTTGTGAGGAGTTGAAGGAGCCATTCTTGGATGACTTTGCGTGTCCCATACAAAGCAGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

176

Amino Acids

19.3

Weight (kDa)

5.42

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 105 1.5e-08 NmrA-like family
Epimerase PF01370 8 - 131 1.5e-13 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 136 6.4e-14 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 129 1.9e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 133 4.3e-08 Male sterility protein
NAD_binding_10 PF13460 12 - 132 7.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 116, 299, 452
AcsI RAATTY 1 cut(s) 350
AfaI GTAC 1 cut(s) 264
AgsI TTSAA 2 cut(s) 184, 481
AluBI AGCT 2 cut(s) 38, 53
AluI AGCT 2 cut(s) 38, 53
AlwI GGATC 3 cut(s) 116, 299, 452
AoxI GGCC 2 cut(s) 4, 525
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 4, 525
AsuHPI GGTGA 1 cut(s) 76
BbsI GAAGAC 1 cut(s) 418
BbvI GCAGC 1 cut(s) 379
BccI CCATC 2 cut(s) 149, 221
BfaI CTAG 1 cut(s) 529
BfmI CTRYAG 2 cut(s) 309, 393
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
BmgT120I GGNCC 2 cut(s) 4, 525
BmiI GGNNCC 1 cut(s) 486
BmsI GCATC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 418
BsaJI CCNNGG 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 236, 502
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 2 cut(s) 6, 527
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 495
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 2 cut(s) 6, 527
Bsp143I GATC 3 cut(s) 121, 304, 457
Bsp19I CCATGG 1 cut(s) 387
BspANI GGCC 2 cut(s) 6, 527
BspLI GGNNCC 1 cut(s) 486
BspMAI CTGCAG 2 cut(s) 313, 397
BspPI GGATC 3 cut(s) 116, 299, 452
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 3 cut(s) 121, 304, 457
BssT1I CCWWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 236, 502
BstKTI GATC 3 cut(s) 124, 307, 460
BstMBI GATC 3 cut(s) 121, 304, 457
BstSFI CTRYAG 2 cut(s) 309, 393
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 418
BsuRI GGCC 2 cut(s) 6, 527
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 236, 502
Cfr13I GGNCC 2 cut(s) 4, 525
Csp6I GTAC 1 cut(s) 263
CviAII CATG 3 cut(s) 57, 388, 447
CviJI RGCY 9 cut(s) 6, 38, 53, 61, 101, 172, 392, 487, 527
CviKI_1 RGCY 9 cut(s) 6, 38, 53, 61, 101, 172, 392, 487, 527
CviQI GTAC 1 cut(s) 263
DpnI GATC 3 cut(s) 123, 306, 459
DpnII GATC 3 cut(s) 121, 304, 457
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 3 cut(s) 60, 391, 450
FaiI YATR 7 cut(s) 50, 58, 90, 249, 389, 448, 515
FaqI GGGAC 1 cut(s) 495
FatI CATG 3 cut(s) 56, 387, 446
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 2 cut(s) 243, 509
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 1 cut(s) 529
GluI GCNGC 1 cut(s) 393
HaeIII GGCC 2 cut(s) 6, 527
HapII CCGG 1 cut(s) 260
Hin1II CATG 3 cut(s) 60, 391, 450
HinfI GANTC 1 cut(s) 70
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 2 cut(s) 110, 364
HpyAV CCTTC 3 cut(s) 10, 230, 475
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 2 cut(s) 311, 395
Hsp92II CATG 3 cut(s) 60, 391, 450
Kzo9I GATC 3 cut(s) 121, 304, 457
LmnI GCTCC 3 cut(s) 35, 158, 484
LpnPI CCDG 7 cut(s) 18, 27, 47, 273, 275, 321, 508
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 529
MaeIII GTNAC 3 cut(s) 28, 75, 110
MalI GATC 3 cut(s) 123, 306, 459
MboI GATC 3 cut(s) 121, 304, 457
MboII GAAGA 4 cut(s) 79, 212, 375, 423
MluCI AATT 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 380
MnlI CCTC 6 cut(s) 6, 77, 196, 360, 433, 466
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 4 cut(s) 327, 336, 377, 419
MspI CCGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 387
NdeII GATC 3 cut(s) 121, 304, 457
NlaIII CATG 3 cut(s) 60, 391, 450
NlaIV GGNNCC 1 cut(s) 486
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 2 cut(s) 4, 525
PstI CTGCAG 2 cut(s) 313, 397
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 327, 336, 377, 419
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 3 cut(s) 121, 304, 457
Sau96I GGNCC 2 cut(s) 4, 525
SetI ASST 8 cut(s) 21, 40, 55, 88, 219, 241, 418, 425
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 2 cut(s) 309, 393
Sse9I AATT 1 cut(s) 350
SspMI CTAG 1 cut(s) 529
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 106
TasI AATT 1 cut(s) 350
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 4 cut(s) 327, 336, 377, 419
Tru9I TTAA 4 cut(s) 327, 336, 377, 419
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 5 cut(s) 37, 45, 164, 261, 456
XapI RAATTY 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.