Rorug04G0048200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003476
Physical Location & Seq
Forward (+)
7461063 .. 7461497
435 bp
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UTR
Exon/CDS
Intron
Rorug04G0048200.1

Sequence Viewer

Length: 435 bp
ATGGAACCACAAACCCAGCAACCAACCGAGGATGGAGGAAGCAACAAAGCAGTCGGGAGTAGTGCTAACATGCTTTGCAGGCGGAGCAGTACTAGGTGGACTCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTACTACAACAACAGAGTTAGGTCCCCAACTGTAGAGCAGGTTCAGAGGATCTGTCTCCAGCTGAAACAGTACGGAAAGGTTGAGGGCAAGAACGTCTTTTATTGGTTCCAGAACCACAAGGCTCGGGAGAAGCAGAGGAAGAGGTCCACTTCGAATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTAATGGTAATGGTACCAATTGGAAACCTGAGGATCAGTATATTAACTTCGGATCTTCTGCATCTGCTTCTGCTTCTTCAGCTGGTATGATTTCTTTTACGCTATAA

Protein Analysis

144

Amino Acids

16.26

Weight (kDa)

10.01

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 31 - 91 6.8e-12 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 166
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 341
AciI CCGC 1 cut(s) 82
AclWI GGATC 3 cut(s) 194, 369, 388
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 3 cut(s) 91, 209, 343
AluBI AGCT 3 cut(s) 136, 199, 410
AluI AGCT 3 cut(s) 136, 199, 410
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 3 cut(s) 194, 369, 388
Ama87I CYCGRG 1 cut(s) 261
Asp718I GGTACC 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 159, 282
AsuII TTCGAA 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 159, 282
AxyI CCTNAGG 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 341
BarI GAAGNNNNNNTAC 2 cut(s) 359, 391
BccI CCATC 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 108, 168
BfuAI ACCTGC 1 cut(s) 166
BmcAI AGTACT 1 cut(s) 91
Bme18I GGWCC 2 cut(s) 159, 282
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 159, 282
BmiI GGNNCC 4 cut(s) 6, 161, 245, 343
BmsI GCATC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 179
Bpu14I TTCGAA 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 27
Bse21I CCTNAGG 1 cut(s) 357
Bse8I GATNNNNATC 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 37
BseJI GATNNNNATC 1 cut(s) 122
BseMII CTCAG 1 cut(s) 348
BseSI GKGCMC 1 cut(s) 306
BseYI CCCAGC 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 341
BsiHKCI CYCGRG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 145
BsoBI CYCGRG 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 5 cut(s) 112, 186, 314, 361, 380
BspACI CCGC 1 cut(s) 82
BspCNI CTCAG 1 cut(s) 349
BspLI GGNNCC 4 cut(s) 6, 161, 245, 343
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 3 cut(s) 194, 369, 388
BspT104I TTCGAA 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 5 cut(s) 112, 186, 314, 361, 380
Bst4CI ACNGT 2 cut(s) 169, 207
Bst6I CTCTTC 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 37
BstKTI GATC 5 cut(s) 115, 189, 317, 364, 383
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 5 cut(s) 112, 186, 314, 361, 380
BstMWI GCNNNNNNNGC 3 cut(s) 79, 84, 407
BstNSI RCATGY 1 cut(s) 73
BstSFI CTRYAG 2 cut(s) 108, 168
BstSLI GKGCMC 1 cut(s) 306
BstX2I RGATCY 2 cut(s) 186, 380
BstYI RGATCY 2 cut(s) 186, 380
Bsu36I CCTNAGG 1 cut(s) 357
BtsCI GGATG 1 cut(s) 37
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 159, 282
Csp6I GTAC 3 cut(s) 90, 208, 342
CviAII CATG 3 cut(s) 70, 299, 307
CviJI RGCY 5 cut(s) 136, 199, 260, 322, 410
CviKI_1 RGCY 5 cut(s) 136, 199, 260, 322, 410
CviQI GTAC 3 cut(s) 90, 208, 342
DdeI CTNAG 1 cut(s) 357
DpnI GATC 5 cut(s) 114, 188, 316, 363, 382
DpnII GATC 5 cut(s) 112, 186, 314, 361, 380
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
EciI GGCGGA 1 cut(s) 97
Eco47I GGWCC 2 cut(s) 159, 282
Eco57I CTGAAG 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 159
FaeI CATG 3 cut(s) 73, 302, 310
FaiI YATR 6 cut(s) 71, 300, 308, 369, 416, 433
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 145
FatI CATG 3 cut(s) 69, 298, 306
FokI GGATG 1 cut(s) 44
FspBI CTAG 1 cut(s) 93
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 179
Hin1II CATG 3 cut(s) 73, 302, 310
HinfI GANTC 2 cut(s) 100, 123
Hpy166II GTNNAC 2 cut(s) 99, 285
Hpy188I TCNGA 3 cut(s) 117, 183, 380
Hpy188III TCNNGA 3 cut(s) 55, 247, 263
Hpy8I GTNNAC 2 cut(s) 99, 285
HpyCH4III ACNGT 2 cut(s) 169, 207
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 78, 310, 389
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 84, 407
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 3 cut(s) 73, 302, 310
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 5 cut(s) 112, 186, 314, 361, 380
LmnI GCTCC 2 cut(s) 84, 133
LpnPI CCDG 8 cut(s) 29, 64, 161, 209, 260, 303, 369, 396
LweI GCATC 1 cut(s) 398
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 231
MalI GATC 5 cut(s) 114, 188, 316, 363, 382
MboI GATC 5 cut(s) 112, 186, 314, 361, 380
MboII GAAGA 3 cut(s) 289, 375, 396
MfeI CAATTG 1 cut(s) 346
MflI RGATCY 2 cut(s) 186, 380
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 1 cut(s) 346
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 8 cut(s) 22, 29, 137, 177, 214, 267, 273, 352
MseI TTAA 1 cut(s) 372
MspA1I CMGCKG 2 cut(s) 199, 410
MunI CAATTG 1 cut(s) 346
MwoI GCNNNNNNNGC 3 cut(s) 79, 84, 407
NdeII GATC 5 cut(s) 112, 186, 314, 361, 380
NlaIII CATG 3 cut(s) 73, 302, 310
NlaIV GGNNCC 4 cut(s) 6, 161, 245, 343
NspI RCATGY 1 cut(s) 73
NspV TTCGAA 1 cut(s) 290
PfeI GAWTC 1 cut(s) 123
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PpuMI RGGWCCY 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 4 cut(s) 6, 161, 245, 343
PspPI GGNCC 2 cut(s) 159, 282
PspPPI RGGWCCY 1 cut(s) 159
PsrI GAACNNNNNNTAC 2 cut(s) 162, 194
PsuI RGATCY 2 cut(s) 186, 380
PvuII CAGCTG 2 cut(s) 199, 410
RsaI GTAC 3 cut(s) 91, 209, 343
RsaNI GTAC 3 cut(s) 90, 208, 342
SaqAI TTAA 1 cut(s) 372
Sau3AI GATC 5 cut(s) 112, 186, 314, 361, 380
Sau96I GGNCC 2 cut(s) 159, 282
ScaI AGTACT 1 cut(s) 91
SchI GAGTC 1 cut(s) 94
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 2 cut(s) 108, 168
SfuI TTCGAA 1 cut(s) 290
SinI GGWCC 2 cut(s) 159, 282
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 346
SsiI CCGC 1 cut(s) 82
SspMI CTAG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 169, 207
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 290
TasI AATT 1 cut(s) 346
TatI WGTACW 1 cut(s) 89
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 1 cut(s) 225
VpaK11BI GGWCC 2 cut(s) 159, 282
XceI RCATGY 1 cut(s) 73
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.