MD07G1021100.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003476
Physical Location & Seq
Reverse (-)
1794443 .. 1795799
1357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1021100.v1.1.491

Sequence Viewer

Length: 429 bp
ATGAATTGGTGTGCTACGCTCGGCCTAGCTGAACTGCCAATTGAAACCTCCTCAATAATTCTGTTTCTGCAAAACACAGATGATCGAAAGAAAACAGAAGACGTACACTCACTAGATGGAGCAAGAGAAAGGCTTCATTTGTTGAAAGTGGATTTGTTAGGAGCTTTCGATACTGTAGATGATGGATGTGTAGGTGTTTTTCATACAGCATTGTCTGCACAATTCTCAGCCACTGACCCACAGGCAGAAATAGTTGAGCCTGCAGTGAAAGGAACACTCAATGTTCTTGAATCGTGCGCAAAATTCCCCTCTGTCAAGAGAGTGGTTTTAACATCATCTATTGCTTCAGTAAAGGGGACTGGAACCCCTCTGGCATCTGATGTGCGATGGTATCCTCTCTCAAAAACTTTAGCTGAGGAGGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD

Protein Analysis

143

Amino Acids

15.24

Weight (kDa)

4.96

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 47 - 119 5.2e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 298
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 105
AgsI TTSAA 3 cut(s) 44, 145, 290
AluBI AGCT 3 cut(s) 29, 164, 413
AluI AGCT 3 cut(s) 29, 164, 413
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 132
AspLEI GCGC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 1 cut(s) 409
BccI CCATC 3 cut(s) 110, 176, 381
BciVI GTATCC 1 cut(s) 402
BfaI CTAG 2 cut(s) 26, 113
BfmI CTRYAG 2 cut(s) 174, 261
BfuI GTATCC 1 cut(s) 402
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 364
BmsI GCATC 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 414
Bse1I ACTGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 191
BseMII CTCAG 2 cut(s) 240, 405
BseNI ACTGG 1 cut(s) 364
BseRI GAGGAG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 1 cut(s) 24
BslFI GGGAC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 1 cut(s) 24
Bsp143I GATC 1 cut(s) 82
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 2 cut(s) 239, 406
BspLI GGNNCC 1 cut(s) 364
BspMAI CTGCAG 1 cut(s) 265
BsrI ACTGG 1 cut(s) 364
BssMI GATC 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 175
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 2 cut(s) 226, 414
BstF5I GGATG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 2 cut(s) 215, 419
BstSFI CTRYAG 2 cut(s) 174, 261
BstV1I GCAGC 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 105
BsuI GTATCC 1 cut(s) 402
BsuRI GGCC 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 400
BtsCI GGATG 1 cut(s) 191
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 231, 270
Cac8I GCNNGC 1 cut(s) 261
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 104
CviJI RGCY 8 cut(s) 24, 29, 133, 164, 230, 259, 413, 422
CviKI_1 RGCY 8 cut(s) 24, 29, 133, 164, 230, 259, 413, 422
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 2 cut(s) 226, 414
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 330
FaiI YATR 1 cut(s) 204
FaqI GGGAC 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 2 cut(s) 26, 113
FspI TGCGCA 1 cut(s) 298
GlaI GCGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 24
HhaI GCGC 1 cut(s) 299
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 379
Hpy188III TCNNGA 2 cut(s) 287, 316
Hpy8I GTNNAC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 3 cut(s) 70, 218, 263
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 419
HpyF3I CTNAG 2 cut(s) 226, 414
HpySE526I ACGT 1 cut(s) 102
HspAI GCGC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 119, 161
LpnPI CCDG 4 cut(s) 227, 273, 345, 356
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 1 cut(s) 383
MaeI CTAG 2 cut(s) 26, 113
MaeII ACGT 1 cut(s) 102
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 110
MfeI CAATTG 1 cut(s) 39
MluCI AATT 5 cut(s) 4, 39, 57, 221, 302
MnlI CCTC 7 cut(s) 58, 61, 319, 378, 405, 409, 412
MroXI GAANNNNTTC 1 cut(s) 132
MseI TTAA 1 cut(s) 329
MunI CAATTG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 215, 419
NdeII GATC 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 364
NsbI TGCGCA 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 364
PstI CTGCAG 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 233
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 1 cut(s) 82
SetI ASST 6 cut(s) 31, 50, 105, 166, 196, 415
SfaNI GCATC 1 cut(s) 383
SfcI CTRYAG 2 cut(s) 174, 261
Sse9I AATT 5 cut(s) 4, 39, 57, 221, 302
SspMI CTAG 2 cut(s) 26, 113
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 85, 168
TasI AATT 5 cut(s) 4, 39, 57, 221, 302
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 2 cut(s) 238, 270
TseI GCWGC 1 cut(s) 422
TspDTI ATGAA 3 cut(s) 17, 125, 191
TspRI CASTG 2 cut(s) 238, 270
XapI RAATTY 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 2 cut(s) 26, 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.