MD02G1305000.v1.1

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003476
Physical Location & Seq
Forward (+)
35824002 .. 35826292
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1305000.v1.1.491

Sequence Viewer

Length: 510 bp
ATGAGTGGAGAAAGAGGGGTGGTGTGTGTAACAGGAGCCTCAGGTTACATAGCATCATGGTTGGTGAAGCTCTTGCTACAAAGGGGTTATGCTGTCAGAGCCACTGTTCGCGACCCAAGTGATCCAAAGAAAACAGAACACTTGCTCTTACTAGATGGAGCAAAAGAAAGGCTTCATTTGTTTAGAGCAGATTTATTAGAAGAAGGATCTTTTGACACTGTAGTTGATGGATGTCGAGGCGTTTTTCATACAGCATCTCCTGTCCAGTTTTCAGTCACAGACCCGCAGGCAGAACTAGTTGAGCCTGCAGTGAAGGGAACAACACTTAATATTCTAAAATCATGTGCGAAATTTCCTACTGTCAAGAGAGTAGTTTTAACATCTTCTACGGCTTCAGTATCGGTGATTGGGAAACCTCTGACCCCTGGTGTGGTTTTGGATGAAACATGGTATTCGAATCCACTTGATTGTGAGAAGATCAAAACTGGTATGCGCTCTAAAAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

170

Amino Acids

18.32

Weight (kDa)

8.91

Isoelectric Point (pI)

24.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 103 2e-09 NmrA-like family
Epimerase PF01370 8 - 137 1e-13 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 137 1.1e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 131 1.2e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 133 8.7e-07 Male sterility protein
NAD_binding_10 PF13460 12 - 131 1.6e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 111
AciI CCGC 1 cut(s) 284
AclWI GGATC 2 cut(s) 116, 214
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 430
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
AlwI GGATC 2 cut(s) 116, 214
ApoI RAATTY 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 171
AspLEI GCGC 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 76, 415
AsuII TTCGAA 1 cut(s) 455
AxyI CCTNAGG 1 cut(s) 40
BccI CCATC 2 cut(s) 149, 221
BceAI ACGGC 1 cut(s) 405
BciT130I CCWGG 1 cut(s) 426
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 2 cut(s) 152, 296
BfmI CTRYAG 2 cut(s) 219, 306
Bme1390I CCNGG 1 cut(s) 426
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 62, 263
Bpu14I TTCGAA 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 2 cut(s) 265, 490
Bse21I CCTNAGG 1 cut(s) 40
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 2 cut(s) 236, 445
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 265, 490
Bsh1236I CGCG 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 455
Bsp143I GATC 3 cut(s) 121, 206, 477
Bsp68I TCGCGA 1 cut(s) 111
BspACI CCGC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 53
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 37
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 2 cut(s) 116, 214
BspT104I TTCGAA 1 cut(s) 455
BsrI ACTGG 2 cut(s) 265, 490
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 3 cut(s) 121, 206, 477
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 106, 220, 361
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 288, 306
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 236, 445
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 3 cut(s) 124, 209, 480
BstMBI GATC 3 cut(s) 121, 206, 477
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 2 cut(s) 219, 306
BstUI CGCG 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
Bsu36I CCTNAGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 236, 445
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 3 cut(s) 102, 216, 315
BtuMI TCGCGA 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 288, 306
CfoI GCGC 1 cut(s) 495
CviAII CATG 3 cut(s) 57, 342, 447
CviJI RGCY 6 cut(s) 38, 70, 101, 172, 304, 392
CviKI_1 RGCY 6 cut(s) 38, 70, 101, 172, 304, 392
DdeI CTNAG 1 cut(s) 40
DpnI GATC 3 cut(s) 123, 208, 479
DpnII GATC 3 cut(s) 121, 206, 477
Eco57I CTGAAG 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 424
FaeI CATG 3 cut(s) 60, 345, 450
FaiI YATR 7 cut(s) 50, 58, 90, 249, 343, 448, 491
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 3 cut(s) 56, 341, 446
FauI CCCGC 1 cut(s) 291
FokI GGATG 2 cut(s) 243, 452
FspBI CTAG 2 cut(s) 152, 296
GlaI GCGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 495
Hin1II CATG 3 cut(s) 60, 345, 450
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 1 cut(s) 457
HphI GGTGA 2 cut(s) 76, 415
Hpy188I TCNGA 2 cut(s) 98, 420
Hpy188III TCNNGA 2 cut(s) 110, 364
HpyAV CCTTC 2 cut(s) 197, 307
HpyCH4III ACNGT 3 cut(s) 106, 220, 361
HpyCH4V TGCA 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 1 cut(s) 40
Hsp92II CATG 3 cut(s) 60, 345, 450
HspAI GCGC 1 cut(s) 493
Kzo9I GATC 3 cut(s) 121, 206, 477
LmnI GCTCC 2 cut(s) 35, 158
LpnPI CCDG 9 cut(s) 18, 27, 272, 273, 278, 318, 411, 438, 471
LweI GCATC 2 cut(s) 62, 263
MaeI CTAG 2 cut(s) 152, 296
MaeIII GTNAC 3 cut(s) 28, 44, 274
MalI GATC 3 cut(s) 123, 208, 479
MboI GATC 3 cut(s) 121, 206, 477
MboII GAAGA 3 cut(s) 212, 375, 487
MflI RGATCY 1 cut(s) 206
MluCI AATT 1 cut(s) 350
MnlI CCTC 4 cut(s) 8, 49, 230, 426
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 2 cut(s) 327, 377
MspR9I CCNGG 1 cut(s) 426
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 3 cut(s) 121, 206, 477
NlaIII CATG 3 cut(s) 60, 345, 450
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 274
NruI TCGCGA 1 cut(s) 111
NspV TTCGAA 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 457
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 206
RruI TCGCGA 1 cut(s) 111
SaqAI TTAA 2 cut(s) 327, 377
Sau3AI GATC 3 cut(s) 121, 206, 477
ScrFI CCNGG 1 cut(s) 426
SetI ASST 3 cut(s) 46, 72, 418
SfaNI GCATC 2 cut(s) 62, 263
SfcI CTRYAG 2 cut(s) 219, 306
SfuI TTCGAA 1 cut(s) 455
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 1 cut(s) 350
SsiI CCGC 1 cut(s) 284
SspI AATATT 1 cut(s) 331
SspMI CTAG 2 cut(s) 152, 296
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 106, 220, 361
TaqI TCGA 2 cut(s) 235, 455
TasI AATT 1 cut(s) 350
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 2 cut(s) 327, 377
Tru9I TTAA 2 cut(s) 327, 377
TscAI CASTG 3 cut(s) 109, 223, 315
TseFI GTSAC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 164, 236, 456
TspRI CASTG 3 cut(s) 109, 223, 315
XapI RAATTY 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 2 cut(s) 152, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.