Rh1AG028800

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003476
Physical Location & Seq
Reverse (-)
5206916 .. 5207767
852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG028800.1

Sequence Viewer

Length: 552 bp
ATGAACTGCGGAGAAAGCAAGGTTGTGTGTGTGACGGGAGCATCTGGTTACATAGCATCATGGCTGGTGAAGCTCTTGTTGCAACGAGGTTATACTGTCAAAGCCACGGTTCGGGACCCAGACGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTGATACGGCAATTCATGGATGTGAATGTCTTTTCCATACGGCGTCCCCTGTCCTACTCTCAGTCTCATCAACTAATCCGCAGCAAAATCTAGATCAATGCACTATAATGTTGCTAGCTCTTAATCGTTGTCAGGCAGAATTAATTGACCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATAGCAGCAGTTGGATATAATGGAAAACCTCTTACTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCATTTTGTGAAAAAACGAAGGTTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

183

Amino Acids

19.84

Weight (kDa)

6.07

Isoelectric Point (pI)

21.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 87 2.5e-09 NmrA-like family
Epimerase PF01370 9 - 150 5e-10 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 157 6.8e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 104 6.1e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 151 7.3e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AciI CCGC 2 cut(s) 9, 293
AclWI GGATC 2 cut(s) 403, 509
AcyI GRCGYC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 187, 373
AluBI AGCT 2 cut(s) 73, 332
AluI AGCT 2 cut(s) 73, 332
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 2 cut(s) 403, 509
AlwNI CAGNNNCTG 1 cut(s) 518
ApeKI GCWGC 2 cut(s) 295, 449
AseI ATTAAT 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 79
AsuNHI GCTAGC 1 cut(s) 328
AvaII GGWCC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 307, 461
BccI CCATC 1 cut(s) 152
BceAI ACGGC 2 cut(s) 237, 270
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 2 cut(s) 305, 329
BfmI CTRYAG 1 cut(s) 444
BisI GCNGC 2 cut(s) 296, 450
BlsI GCNGC 2 cut(s) 297, 451
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 2 cut(s) 116, 117
BmsI GCATC 2 cut(s) 50, 65
BmtI GCTAGC 1 cut(s) 332
Bsa29I ATCGAT 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 105, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 495
BseDI CCNNGG 2 cut(s) 105, 391
BseGI GGATG 2 cut(s) 239, 437
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 288
BseXI GCAGC 2 cut(s) 307, 461
BshVI ATCGAT 1 cut(s) 495
BslFI GGGAC 2 cut(s) 128, 244
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 283
BsmFI GGGAC 2 cut(s) 128, 244
Bsp143I GATC 4 cut(s) 124, 307, 395, 514
BspACI CCGC 2 cut(s) 9, 293
BspCNI CTCAG 1 cut(s) 287
BspDI ATCGAT 1 cut(s) 495
BspLI GGNNCC 2 cut(s) 116, 117
BspOI GCTAGC 1 cut(s) 332
BspPI GGATC 2 cut(s) 403, 509
BssECI CCNNGG 2 cut(s) 105, 391
BssMI GATC 4 cut(s) 124, 307, 395, 514
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 391
Bst4CI ACNGT 2 cut(s) 97, 109
BstACI GRCGYC 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 274
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 2 cut(s) 239, 437
BstKTI GATC 4 cut(s) 127, 310, 398, 517
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 4 cut(s) 124, 307, 395, 514
BstMWI GCNNNNNNNGC 3 cut(s) 15, 70, 79
BstSFI CTRYAG 1 cut(s) 444
BstV1I GCAGC 2 cut(s) 307, 461
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 495
BsuTUI ATCGAT 1 cut(s) 495
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 2 cut(s) 239, 437
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 495
CseI GACGC 1 cut(s) 246
CviAII CATG 2 cut(s) 60, 230
CviJI RGCY 5 cut(s) 64, 73, 104, 175, 332
CviKI_1 RGCY 5 cut(s) 64, 73, 104, 175, 332
DdeI CTNAG 1 cut(s) 274
DpnI GATC 4 cut(s) 126, 309, 397, 516
DpnII GATC 4 cut(s) 124, 307, 395, 514
Eco130I CCWWGG 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 2 cut(s) 63, 233
FaiI YATR 9 cut(s) 53, 61, 93, 231, 252, 320, 434, 446, 462
FaqI GGGAC 2 cut(s) 128, 244
FatI CATG 2 cut(s) 59, 229
Fnu4HI GCNGC 2 cut(s) 296, 450
FokI GGATG 2 cut(s) 246, 424
Fsp4HI GCNGC 2 cut(s) 296, 450
FspBI CTAG 2 cut(s) 305, 329
GluI GCNGC 2 cut(s) 296, 450
HgaI GACGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 2 cut(s) 63, 233
HinfI GANTC 1 cut(s) 500
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 2 cut(s) 113, 305
HpyAV CCTTC 4 cut(s) 200, 367, 400, 532
HpyCH4III ACNGT 2 cut(s) 97, 109
HpyCH4V TGCA 3 cut(s) 82, 315, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 70, 79
HpyF3I CTNAG 1 cut(s) 274
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 2 cut(s) 63, 233
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 124, 307, 395, 514
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 7 cut(s) 30, 50, 132, 276, 332, 378, 531
Lsp1109I GCAGC 2 cut(s) 307, 461
LweI GCATC 2 cut(s) 50, 65
MaeI CTAG 2 cut(s) 305, 329
MaeIII GTNAC 2 cut(s) 31, 47
MalI GATC 4 cut(s) 126, 309, 397, 516
MboI GATC 4 cut(s) 124, 307, 395, 514
MboII GAAGA 1 cut(s) 215
MflI RGATCY 1 cut(s) 514
MluCI AATT 3 cut(s) 225, 353, 357
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 3 cut(s) 80, 451, 483
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 3 cut(s) 336, 356, 384
MslI CAYNNNNRTG 2 cut(s) 234, 320
MwoI GCNNNNNNNGC 3 cut(s) 15, 70, 79
NdeII GATC 4 cut(s) 124, 307, 395, 514
NheI GCTAGC 1 cut(s) 328
NlaIII CATG 2 cut(s) 63, 233
NlaIV GGNNCC 2 cut(s) 116, 117
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 2 cut(s) 297, 451
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 356
PsiI TTATAA 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 1 cut(s) 115
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 514
RseI CAYNNNNRTG 2 cut(s) 234, 320
SaqAI TTAA 3 cut(s) 336, 356, 384
SatI GCNGC 2 cut(s) 296, 450
Sau3AI GATC 4 cut(s) 124, 307, 395, 514
Sau96I GGNCC 1 cut(s) 115
SetI ASST 8 cut(s) 24, 75, 91, 147, 334, 411, 475, 543
SfaNI GCATC 2 cut(s) 50, 65
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 2 cut(s) 234, 320
Sse9I AATT 3 cut(s) 225, 353, 357
SsiI CCGC 2 cut(s) 9, 293
SspMI CTAG 2 cut(s) 305, 329
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 2 cut(s) 97, 109
TaqI TCGA 1 cut(s) 495
TasI AATT 3 cut(s) 225, 353, 357
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 3 cut(s) 336, 356, 384
Tru9I TTAA 3 cut(s) 336, 356, 384
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 295, 449
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 17, 167, 218, 513
VpaK11BI GGWCC 1 cut(s) 115
VspI ATTAAT 1 cut(s) 356
XbaI TCTAGA 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 2 cut(s) 305, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.