Rh1AG028200

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003476
Physical Location & Seq
Reverse (-)
5094907 .. 5097666
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG028200.1

Sequence Viewer

Length: 537 bp
ATGAGTGGAGCAAGGAAAGTTGCTGTGTGTGTAACTGGAGCGTCTGGTTATATTGCATCATGGCTGGTCAAGCTTTTGTTAGGACGAGGTTATATTGTCAAAGCCACTGTCCGGGACCCAAACGATCCGAAGAAAACAGAACACTTGCTTTCACTCGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTGCTAGATGAAGGATCTTTTGACCCTGTGGTTGATGGATGTGACGGTGTTTTCCATACAGCATCTCCAGTACTATTTTCATCGATCAGTGACCCACAGGCAGAACTAATTGACCCTGCTGTGAAGGGAACACTAAATGTTCTGAAATCATGCAAAAAATTTCCAACCGTCAAGCGAGTGGTTTTGACATCTTCTATGGCTTCAGTAATGATGAATGGAAAACCTCTGACCCCTGATGTTGTGGTGGATGAAACATGGTATTCAGATCCACTTATTGCTGAGAAGCACAAGGAGGTCAATTTGGACCTTTTGTCTGAGGTTGAATGGGGTTTAAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

178

Amino Acids

19.32

Weight (kDa)

5.8

Isoelectric Point (pI)

16.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 106 4.9e-09 NmrA-like family
Epimerase PF01370 9 - 134 2.8e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 139 3.2e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 130 3.3e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 137 1.3e-07 Male sterility protein
NAD_binding_10 PF13460 13 - 134 8.1e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 119, 217, 455
AcsI RAATTY 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 187, 518
AhdI GACNNNNNGTC 1 cut(s) 505
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
AlwI GGATC 3 cut(s) 119, 217, 455
ApoI RAATTY 1 cut(s) 353
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 2 cut(s) 115, 499
AsuC2I CCSGG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 115, 499
BccI CCATC 2 cut(s) 152, 224
BcnI CCSGG 1 cut(s) 113
BfaI CTAG 1 cut(s) 200
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 2 cut(s) 115, 499
BmeRI GACNNNNNGTC 1 cut(s) 505
BmgT120I GGNCC 2 cut(s) 115, 499
BmiI GGNNCC 2 cut(s) 116, 117
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 2 cut(s) 65, 266
BpmI CTGGAG 2 cut(s) 57, 246
BpuMI CCSGG 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 40, 263
BseCI ATCGAT 1 cut(s) 278
BseGI GGATG 2 cut(s) 239, 448
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 465, 501
BseNI ACTGG 2 cut(s) 40, 263
BshVI ATCGAT 1 cut(s) 278
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 128
Bsp143I GATC 4 cut(s) 124, 209, 279, 460
BspCNI CTCAG 2 cut(s) 466, 502
BspDI ATCGAT 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 116, 117
BspPI GGATC 3 cut(s) 119, 217, 455
BsrI ACTGG 2 cut(s) 40, 263
BssMI GATC 4 cut(s) 124, 209, 279, 460
Bst4CI ACNGT 3 cut(s) 109, 242, 364
BstDEI CTNAG 2 cut(s) 474, 510
BstF5I GGATG 2 cut(s) 239, 448
BstKTI GATC 4 cut(s) 127, 212, 282, 463
BstMBI GATC 4 cut(s) 124, 209, 279, 460
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 209, 460
BstYI RGATCY 2 cut(s) 209, 460
Bsu15I ATCGAT 1 cut(s) 278
BsuTUI ATCGAT 1 cut(s) 278
BtsCI GGATG 2 cut(s) 239, 448
BtsIMutI CAGTG 2 cut(s) 105, 289
Cfr13I GGNCC 2 cut(s) 115, 499
ClaI ATCGAT 1 cut(s) 278
CseI GACGC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 266
CviAII CATG 3 cut(s) 60, 345, 450
CviJI RGCY 5 cut(s) 64, 73, 104, 175, 395
CviKI_1 RGCY 5 cut(s) 64, 73, 104, 175, 395
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 2 cut(s) 474, 510
DpnI GATC 4 cut(s) 126, 211, 281, 462
DpnII GATC 4 cut(s) 124, 209, 279, 460
DraI TTTAAA 1 cut(s) 528
DriI GACNNNNNGTC 1 cut(s) 505
Eam1105I GACNNNNNGTC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 115, 499
Eco57I CTGAAG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 115
FaeI CATG 3 cut(s) 63, 348, 453
FaiI YATR 7 cut(s) 51, 61, 93, 252, 346, 392, 451
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 128
FatI CATG 3 cut(s) 59, 344, 449
FokI GGATG 2 cut(s) 246, 455
FspBI CTAG 1 cut(s) 200
GsuI CTGGAG 2 cut(s) 57, 246
HapII CCGG 1 cut(s) 112
HgaI GACGC 1 cut(s) 30
Hin1II CATG 3 cut(s) 63, 348, 453
HindIII AAGCTT 1 cut(s) 71
HpaII CCGG 1 cut(s) 112
Hpy188I TCNGA 6 cut(s) 129, 339, 423, 460, 511, 536
HpyAV CCTTC 2 cut(s) 200, 313
HpyCH4III ACNGT 3 cut(s) 109, 242, 364
HpyCH4V TGCA 2 cut(s) 56, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 2 cut(s) 474, 510
Hsp92II CATG 3 cut(s) 63, 348, 453
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 124, 209, 279, 460
LmnI GCTCC 3 cut(s) 8, 38, 161
LpnPI CCDG 9 cut(s) 21, 30, 50, 125, 234, 276, 278, 324, 441
LweI GCATC 2 cut(s) 65, 266
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 31, 236, 284
MalI GATC 4 cut(s) 126, 211, 281, 462
MboI GATC 4 cut(s) 124, 209, 279, 460
MboII GAAGA 2 cut(s) 142, 378
MflI RGATCY 2 cut(s) 209, 460
MluCI AATT 3 cut(s) 303, 353, 493
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 4 cut(s) 80, 429, 481, 505
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 1 cut(s) 527
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 70
NciI CCSGG 1 cut(s) 113
NdeII GATC 4 cut(s) 124, 209, 279, 460
NlaIII CATG 3 cut(s) 63, 348, 453
NlaIV GGNNCC 2 cut(s) 116, 117
NmuCI GTSAC 2 cut(s) 236, 284
PdmI GAANNNNTTC 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 115
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 2 cut(s) 115, 499
PspPPI RGGWCCY 1 cut(s) 115
PsuI RGATCY 2 cut(s) 209, 460
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 527
Sau3AI GATC 4 cut(s) 124, 209, 279, 460
Sau96I GGNCC 2 cut(s) 115, 499
ScaI AGTACT 1 cut(s) 267
ScrFI CCNGG 1 cut(s) 113
SetI ASST 6 cut(s) 75, 91, 421, 492, 504, 516
SfaNI GCATC 2 cut(s) 65, 266
SinI GGWCC 2 cut(s) 115, 499
Sse9I AATT 3 cut(s) 303, 353, 493
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 111
TaaI ACNGT 3 cut(s) 109, 242, 364
TaqI TCGA 2 cut(s) 156, 278
TasI AATT 3 cut(s) 303, 353, 493
TatI WGTACW 1 cut(s) 265
Tru1I TTAA 1 cut(s) 527
Tru9I TTAA 1 cut(s) 527
TscAI CASTG 2 cut(s) 112, 289
TseFI GTSAC 2 cut(s) 236, 284
Tsp45I GTSAC 2 cut(s) 236, 284
TspDTI ATGAA 5 cut(s) 167, 219, 264, 422, 459
TspRI CASTG 2 cut(s) 112, 289
VpaK11BI GGWCC 2 cut(s) 115, 499
XapI RAATTY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.