pycom03g13000

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003476
Physical Location & Seq
Forward (+)
13912951 .. 13915981
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13000.3

Sequence Viewer

Length: 321 bp
ATGAGCAGCGGAGCGGGGAAGGTCGTGTGCGTCACCGGTGCGTCCGGTTACATTGCTTCATGGCTCGTCAAGCTTCTTCTCCAGCGCGGCTACACCGTCAAGGCCTCCATTCGCGACCCAAATGATCCAACTAAGACAGAGCACTTGCATGCGCTTGATGGAGCTCAAGACAGACTTCAACTTTTCAAAGCAAATCTATTGGAAGAAGGTTCCTTTGACTCTGCTGTTGAGGGTTGTGAAGGGGTATTCCATACCGCATCCCCCTTTTATCATGATGTCACAGACCCAAAGTTCAGGCAGAACTACTTGAACCTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

107

Amino Acids

11.64

Weight (kDa)

6.03

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 14
AccII CGCG 2 cut(s) 87, 114
AciI CCGC 4 cut(s) 9, 14, 87, 255
AclWI GGATC 1 cut(s) 119
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 3 cut(s) 179, 187, 310
AluBI AGCT 2 cut(s) 73, 164
AluI AGCT 2 cut(s) 73, 164
Alw21I GWGCWC 2 cut(s) 144, 166
AlwI GGATC 1 cut(s) 119
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 1 cut(s) 6
AsiGI ACCGGT 1 cut(s) 35
AspLEI GCGC 2 cut(s) 87, 154
AsuHPI GGTGA 1 cut(s) 25
BanII GRGCYC 1 cut(s) 166
Bbv12I GWGCWC 2 cut(s) 144, 166
BbvI GCAGC 1 cut(s) 18
BccI CCATC 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 314
BisI GCNGC 2 cut(s) 7, 88
BlsI GCNGC 2 cut(s) 8, 89
BmiI GGNNCC 1 cut(s) 211
BmsI GCATC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 150
BsaWI WCCGGW 2 cut(s) 35, 44
Bse118I RCCGGY 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 51
BseGI GGATG 1 cut(s) 257
BseMI GCAATG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 18
Bsh1236I CGCG 2 cut(s) 87, 114
BshFI GGCC 1 cut(s) 104
BshTI ACCGGT 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 144, 166
BsiSI CCGG 2 cut(s) 36, 45
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 144, 166
Bsp143I GATC 1 cut(s) 124
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 4 cut(s) 9, 14, 87, 255
BspANI GGCC 1 cut(s) 104
BspFNI CGCG 2 cut(s) 87, 114
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssMI GATC 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 257
BstFNI CGCG 2 cut(s) 87, 114
BstHHI GCGC 2 cut(s) 87, 154
BstKTI GATC 1 cut(s) 127
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstNSI RCATGY 1 cut(s) 152
BstSFI CTRYAG 1 cut(s) 314
BstUI CGCG 2 cut(s) 87, 114
BstV1I GCAGC 1 cut(s) 18
BsuRI GGCC 1 cut(s) 104
BtsCI GGATG 1 cut(s) 257
BtuMI TCGCGA 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 150
CciI TCATGA 1 cut(s) 271
CfoI GCGC 2 cut(s) 87, 154
Cfr10I RCCGGY 1 cut(s) 35
CseI GACGC 2 cut(s) 19, 30
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 3 cut(s) 60, 149, 272
CviJI RGCY 5 cut(s) 64, 73, 90, 104, 164
CviKI_1 RGCY 5 cut(s) 64, 73, 90, 104, 164
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 164
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 3 cut(s) 63, 152, 275
FaiI YATR 4 cut(s) 61, 150, 252, 273
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 3 cut(s) 59, 148, 271
FauI CCCGC 1 cut(s) 7
Fnu4HI GCNGC 2 cut(s) 7, 88
FokI GGATG 1 cut(s) 244
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 2 cut(s) 7, 88
GlaI GCGC 2 cut(s) 86, 153
GluI GCNGC 2 cut(s) 7, 88
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 1 cut(s) 104
HapII CCGG 2 cut(s) 36, 45
HgaI GACGC 2 cut(s) 19, 30
HhaI GCGC 2 cut(s) 87, 154
Hin1II CATG 3 cut(s) 63, 152, 275
Hin6I GCGC 2 cut(s) 85, 152
HinP1I GCGC 2 cut(s) 85, 152
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 1 cut(s) 218
HpaII CCGG 2 cut(s) 36, 45
HphI GGTGA 1 cut(s) 25
Hpy188III TCNNGA 3 cut(s) 113, 167, 272
HpyAV CCTTC 3 cut(s) 13, 200, 233
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 2 cut(s) 148, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 1 cut(s) 132
Hsp92II CATG 3 cut(s) 63, 152, 275
HspAI GCGC 2 cut(s) 85, 152
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 2 cut(s) 11, 161
LpnPI CCDG 4 cut(s) 49, 58, 95, 280
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 31, 47, 277
MalI GATC 1 cut(s) 126
MbiI CCGCTC 1 cut(s) 14
MboI GATC 1 cut(s) 124
MboII GAAGA 2 cut(s) 68, 215
MhlI GDGCHC 2 cut(s) 144, 166
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 2 cut(s) 115, 223
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 2 cut(s) 36, 45
MvnI CGCG 2 cut(s) 87, 114
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 1 cut(s) 124
NlaIII CATG 3 cut(s) 63, 152, 275
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 31, 277
NruI TCGCGA 1 cut(s) 114
NspI RCATGY 1 cut(s) 152
PaeI GCATGC 1 cut(s) 152
PagI TCATGA 1 cut(s) 271
PceI AGGCCT 1 cut(s) 104
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 2 cut(s) 8, 89
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 211
PstI CTGCAG 1 cut(s) 318
RruI TCGCGA 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 166
SatI GCNGC 2 cut(s) 7, 88
Sau3AI GATC 1 cut(s) 124
SchI GAGTC 1 cut(s) 212
SduI GDGCHC 2 cut(s) 144, 166
SetI ASST 5 cut(s) 24, 75, 166, 211, 315
SfaNI GCATC 1 cut(s) 266
SfcI CTRYAG 1 cut(s) 314
SgrAI CRCCGGYG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
SphI GCATGC 1 cut(s) 152
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 4 cut(s) 9, 14, 87, 255
SstI GAGCTC 1 cut(s) 166
StuI AGGCCT 1 cut(s) 104
TaaI ACNGT 1 cut(s) 97
TauI GCSGC 1 cut(s) 90
TseFI GTSAC 2 cut(s) 31, 277
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 2 cut(s) 31, 277
TspDTI ATGAA 1 cut(s) 48
XceI RCATGY 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.