RchiOBHm_Chr1g0350261

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003476
Physical Location & Seq
Forward (+)
43436239 .. 43437043
805 bp
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UTR
Exon/CDS
Intron
PRQ57614

Sequence Viewer

Length: 282 bp
ATGGAACAAGTGAAGTCTGTAACTGGCGGAACTGGATTCATCGGATCATGGCTGGTTATGAAGCTTCTGCAACATGGTTACCCTGTTCGACCTGACCCAGAATGCAAGAGAGATATCAGCTACCTCACAAGCCTACCAAGAGCATCAGAGAAGCTGCAGATCTTCAATGCGGATCTCGACCAGCCCGAAAGCTTCAATGAAGAAATTGAGGGATGCATGCATCGGGGTCTTCCATATATGTTGCTCATCCTATGCCTGACAAAGAACTTGATGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

93

Amino Acids

10.6

Weight (kDa)

6.07

Isoelectric Point (pI)

68.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDP_Man_Dehyd PF16363 7 - 69 2.2e-06 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 27, 170
AclWI GGATC 2 cut(s) 52, 180
AgsI TTSAA 2 cut(s) 166, 196
AluBI AGCT 4 cut(s) 64, 120, 154, 192
AluI AGCT 4 cut(s) 64, 120, 154, 192
AlwI GGATC 2 cut(s) 52, 180
ApeKI GCWGC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 155
BglII AGATCT 1 cut(s) 159
BisI GCNGC 1 cut(s) 155
BlsI GCNGC 1 cut(s) 156
BmsI GCATC 3 cut(s) 152, 203, 229
BpiI GAAGAC 1 cut(s) 221
Bse1I ACTGG 2 cut(s) 28, 37
BseGI GGATG 2 cut(s) 218, 246
BseNI ACTGG 2 cut(s) 28, 37
BseXI GCAGC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 107
Bsp143I GATC 3 cut(s) 44, 159, 172
BspACI CCGC 2 cut(s) 27, 170
BspMAI CTGCAG 1 cut(s) 159
BspPI GGATC 2 cut(s) 52, 180
BsrI ACTGG 2 cut(s) 28, 37
BssMI GATC 3 cut(s) 44, 159, 172
BstC8I GCNNGC 1 cut(s) 218
BstEII GGTNACC 1 cut(s) 77
BstF5I GGATG 2 cut(s) 218, 246
BstKTI GATC 3 cut(s) 47, 162, 175
BstMBI GATC 3 cut(s) 44, 159, 172
BstNSI RCATGY 1 cut(s) 220
BstPI GGTNACC 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 141
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 2 cut(s) 159, 172
BstYI RGATCY 2 cut(s) 159, 172
BtsCI GGATG 2 cut(s) 218, 246
Cac8I GCNNGC 1 cut(s) 218
CviAII CATG 3 cut(s) 48, 74, 217
CviJI RGCY 7 cut(s) 52, 64, 120, 132, 154, 184, 192
CviKI_1 RGCY 7 cut(s) 52, 64, 120, 132, 154, 184, 192
DpnI GATC 3 cut(s) 46, 161, 174
DpnII GATC 3 cut(s) 44, 159, 172
EciI GGCGGA 1 cut(s) 42
Eco32I GATATC 1 cut(s) 115
Eco91I GGTNACC 1 cut(s) 77
EcoO65I GGTNACC 1 cut(s) 77
EcoRV GATATC 1 cut(s) 115
EcoT22I ATGCAT 2 cut(s) 218, 222
FaeI CATG 3 cut(s) 51, 77, 220
FaiI YATR 8 cut(s) 49, 59, 75, 218, 235, 237, 239, 253
FatI CATG 3 cut(s) 47, 73, 216
Fnu4HI GCNGC 1 cut(s) 155
FokI GGATG 2 cut(s) 225, 233
Fsp4HI GCNGC 1 cut(s) 155
GluI GCNGC 1 cut(s) 155
Hin1II CATG 3 cut(s) 51, 77, 220
HindIII AAGCTT 2 cut(s) 62, 190
HinfI GANTC 1 cut(s) 36
Hpy188I TCNGA 2 cut(s) 44, 148
Hpy188III TCNNGA 1 cut(s) 176
HpyCH4V TGCA 5 cut(s) 70, 105, 157, 216, 220
Hsp92II CATG 3 cut(s) 51, 77, 220
Kzo9I GATC 3 cut(s) 44, 159, 172
LpnPI CCDG 8 cut(s) 9, 18, 38, 96, 105, 111, 194, 269
Lsp1109I GCAGC 1 cut(s) 141
LweI GCATC 3 cut(s) 152, 203, 229
MaeIII GTNAC 2 cut(s) 19, 77
MalI GATC 3 cut(s) 46, 161, 174
MboI GATC 3 cut(s) 44, 159, 172
MboII GAAGA 3 cut(s) 154, 212, 221
MflI RGATCY 2 cut(s) 159, 172
MluCI AATT 1 cut(s) 204
MnlI CCTC 2 cut(s) 134, 202
Mph1103I ATGCAT 2 cut(s) 218, 222
Mva1269I GAATGC 1 cut(s) 107
NdeII GATC 3 cut(s) 44, 159, 172
NlaIII CATG 3 cut(s) 51, 77, 220
NsiI ATGCAT 2 cut(s) 218, 222
NspI RCATGY 1 cut(s) 220
PaeI GCATGC 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 1 cut(s) 156
PspEI GGTNACC 1 cut(s) 77
PstI CTGCAG 1 cut(s) 159
PsuI RGATCY 2 cut(s) 159, 172
SatI GCNGC 1 cut(s) 155
Sau3AI GATC 3 cut(s) 44, 159, 172
SetI ASST 6 cut(s) 66, 94, 122, 126, 156, 194
SfaNI GCATC 3 cut(s) 152, 203, 229
SfcI CTRYAG 1 cut(s) 155
SphI GCATGC 1 cut(s) 220
Sse9I AATT 1 cut(s) 204
SsiI CCGC 2 cut(s) 27, 170
TaqI TCGA 2 cut(s) 88, 177
TasI AATT 1 cut(s) 204
TfiI GAWTC 1 cut(s) 36
TseI GCWGC 1 cut(s) 154
TspDTI ATGAA 3 cut(s) 28, 74, 213
XceI RCATGY 1 cut(s) 220
Zsp2I ATGCAT 2 cut(s) 218, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.