Rh1AG028500

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003476
Physical Location & Seq
Reverse (-)
5122800 .. 5136170
13371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG028500.1

Sequence Viewer

Length: 1035 bp
ATGAGTTGTGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTGGTCAATCTCTTATTGCGACGAGGTTTTACTGTCAAAGCCACTGTCCGTGACCCAAATGATCAAAATAATACAGAACACCTACTCTTACTTGACGGAGCAAAAGATAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTGATACGGCAATTGATGGATGTCAATGTGTTTTCCATACGGCATCCCCTGTACTTCTTTCAGTCACTGATCCACAGGCAGAATTAATTGATCCTGCTTTGAAGGGAACACTTGATGTCCTCAGATCATGTGCAAATACTCCGTCTGTGAAAAGGGTGGTTTTAACATCCTCTATTGTATCAGTTATGTTCAACGGAAAACCTCTTTCTCCTGATGTTACAGTTGATGAAACTTGGTTTTCAGATCAAACTTTCTGTGAAAAAGCAAAGCTAAGCTATGCCACTGTCAATGATCATGACGCAGGTGCTGATTGCAACGCGTTTGACTTTGGAGCCTCCGGTTACATAGCATCGTGGCTAGTGAAGCTCTTATTACAACAAGGTTATACTGTCAAAGCCACCGTTCGTGACCCAAATGATGCAAAGAAAACAGAACACTTGCTTTCACTTGATGGAGCTAAGGAAAGGCTTCATCTGTTCAAAGCAGATTTATTAGAAGAAGAATCTTTTGACCCTGTCGTTGATGGATGCGAAGGTGTTTTTCATACAGCATCCCCTGTCCTATTGTCATCTACTAATCCGCAGGCAGAACTGATTGACCCTGCAGTAAAGGGAACACATAATGTTCTGAAATCATGTGCAAAATTTCCGACTGTCAAGAGAGTGGTTTTAACTTCTTCTATGGCTGCAGTAATATTGACCGGAAAACCTCTGACCTCAGATGTGGTAGTTGATGAAACATGGTTTTCGGATCCACTTTTTTGTGAGAAGAATAAGGTACAACTTTGTGTTCTACGTTTTGTGAGAAGTATAACTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

344

Amino Acids

37.38

Weight (kDa)

5.3

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 104 1.6e-08 NmrA-like family
Epimerase PF01370 9 - 134 7.2e-13 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 156 1.1e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 9.3e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 135 4.7e-08 Male sterility protein
NAD_binding_10 PF13460 13 - 130 1.2e-08 NAD(P)H-binding
NmrA PF05368 181 - 274 3.1e-08 NmrA-like family
3Beta_HSD PF01073 182 - 304 7.1e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 182 - 300 6.8e-12 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 182 - 301 3.3e-11 NAD(P)H-binding
GDP_Man_Dehyd PF16363 182 - 298 1.2e-09 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 182 - 303 1.1e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 506
Acc36I ACCTGC 1 cut(s) 506
AccII CGCG 1 cut(s) 533
AciI CCGC 1 cut(s) 794
AclWI GGATC 4 cut(s) 278, 299, 959, 972
AcsI RAATTY 1 cut(s) 857
AfaI GTAC 2 cut(s) 267, 993
AflIII ACRYGT 1 cut(s) 531
AgsI TTSAA 4 cut(s) 187, 316, 406, 694
AluBI AGCT 4 cut(s) 484, 489, 580, 671
AluI AGCT 4 cut(s) 484, 489, 580, 671
AlwI GGATC 4 cut(s) 278, 299, 959, 972
AlwNI CAGNNNCTG 2 cut(s) 281, 521
ApeKI GCWGC 1 cut(s) 899
ApoI RAATTY 1 cut(s) 857
ArsI GACNNNNNNTTYG 4 cut(s) 93, 125, 341, 373
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 681
BamHI GGATCC 1 cut(s) 964
BbvI GCAGC 1 cut(s) 886
BccI CCATC 3 cut(s) 224, 659, 731
BceAI ACGGC 2 cut(s) 237, 270
BclI TGATCA 2 cut(s) 124, 505
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 2 cut(s) 816, 900
BfuAI ACCTGC 1 cut(s) 506
BisI GCNGC 1 cut(s) 900
BlpI GCTNAGC 1 cut(s) 485
BlsI GCNGC 1 cut(s) 901
BmiI GGNNCC 2 cut(s) 547, 966
BmsI GCATC 7 cut(s) 50, 65, 266, 572, 622, 731, 773
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 485
BsaWI WCCGGW 2 cut(s) 551, 914
BseGI GGATG 5 cut(s) 239, 257, 380, 746, 764
BseMII CTCAG 2 cut(s) 349, 945
BseXI GCAGC 1 cut(s) 886
Bsh1236I CGCG 1 cut(s) 533
BsiSI CCGG 2 cut(s) 552, 915
Bsp143I GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
Bsp1720I GCTNAGC 1 cut(s) 485
BspACI CCGC 1 cut(s) 794
BspCNI CTCAG 2 cut(s) 348, 944
BspFNI CGCG 1 cut(s) 533
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 547, 966
BspMAI CTGCAG 2 cut(s) 820, 904
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 4 cut(s) 278, 299, 959, 972
BssMI GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
Bst4CI ACNGT 8 cut(s) 97, 109, 436, 499, 604, 616, 868, 1030
BstC8I GCNNGC 1 cut(s) 798
BstDEI CTNAG 4 cut(s) 335, 485, 672, 931
BstF5I GGATG 5 cut(s) 239, 257, 380, 746, 764
BstFNI CGCG 1 cut(s) 533
BstKTI GATC 7 cut(s) 127, 286, 307, 341, 460, 508, 967
BstMBI GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstSFI CTRYAG 2 cut(s) 816, 900
BstUI CGCG 1 cut(s) 533
BstV1I GCAGC 1 cut(s) 886
BstX2I RGATCY 1 cut(s) 964
BstYI RGATCY 1 cut(s) 964
BtsCI GGATG 5 cut(s) 239, 257, 380, 746, 764
BtsIMutI CAGTG 3 cut(s) 105, 279, 495
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 1 cut(s) 798
CaiI CAGNNNCTG 2 cut(s) 281, 521
CciI TCATGA 1 cut(s) 508
CseI GACGC 1 cut(s) 521
Csp6I GTAC 2 cut(s) 266, 992
CviAII CATG 5 cut(s) 60, 342, 509, 849, 954
CviQI GTAC 2 cut(s) 266, 992
DdeI CTNAG 4 cut(s) 335, 485, 672, 931
DpnI GATC 7 cut(s) 126, 285, 306, 340, 459, 507, 966
DpnII GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
FaeI CATG 5 cut(s) 63, 345, 512, 852, 957
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 5 cut(s) 59, 341, 508, 848, 953
FbaI TGATCA 2 cut(s) 124, 505
Fnu4HI GCNGC 1 cut(s) 900
FokI GGATG 5 cut(s) 244, 246, 367, 751, 753
Fsp4HI GCNGC 1 cut(s) 900
FspBI CTAG 1 cut(s) 572
GluI GCNGC 1 cut(s) 900
HapII CCGG 2 cut(s) 552, 915
HgaI GACGC 1 cut(s) 521
Hin1II CATG 5 cut(s) 63, 345, 512, 852, 957
HinfI GANTC 1 cut(s) 716
HpaII CCGG 2 cut(s) 552, 915
Hpy188I TCNGA 7 cut(s) 338, 457, 843, 864, 927, 934, 964
Hpy188III TCNNGA 4 cut(s) 425, 509, 620, 871
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 3 cut(s) 200, 310, 740
HpyCH4III ACNGT 8 cut(s) 97, 109, 436, 499, 604, 616, 868, 1030
HpyCH4IV ACGT 1 cut(s) 1009
HpyCH4V TGCA 6 cut(s) 347, 528, 635, 818, 854, 902
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 4 cut(s) 335, 485, 672, 931
HpySE526I ACGT 1 cut(s) 1009
Hsp92II CATG 5 cut(s) 63, 345, 512, 852, 957
Ksp22I TGATCA 2 cut(s) 124, 505
Kzo9I GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
LmnI GCTCC 4 cut(s) 38, 161, 545, 668
Lsp1109I GCAGC 1 cut(s) 886
LweI GCATC 7 cut(s) 50, 65, 266, 572, 622, 731, 773
MaeI CTAG 1 cut(s) 572
MaeII ACGT 1 cut(s) 1009
MaeIII GTNAC 6 cut(s) 31, 113, 277, 430, 554, 620
MalI GATC 7 cut(s) 126, 285, 306, 340, 459, 507, 966
MboI GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
MboII GAAGA 5 cut(s) 215, 722, 725, 882, 994
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 1 cut(s) 964
MluCI AATT 4 cut(s) 225, 296, 300, 857
MluI ACGCGT 1 cut(s) 531
MmeI TCCRAC 1 cut(s) 887
MnlI CCTC 7 cut(s) 80, 344, 394, 426, 559, 933, 940
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 3 cut(s) 299, 377, 884
MspI CCGG 2 cut(s) 552, 915
MunI CAATTG 1 cut(s) 225
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 1 cut(s) 577
NdeII GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
NlaIII CATG 5 cut(s) 63, 345, 512, 852, 957
NlaIV GGNNCC 2 cut(s) 547, 966
NmuCI GTSAC 4 cut(s) 31, 113, 277, 620
PagI TCATGA 1 cut(s) 508
PaqCI CACCTGC 1 cut(s) 506
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 1 cut(s) 716
PflFI GACNNNGTC 1 cut(s) 728
PkrI GCNGC 1 cut(s) 901
PshBI ATTAAT 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 547, 966
PstI CTGCAG 2 cut(s) 820, 904
PstNI CAGNNNCTG 2 cut(s) 281, 521
PsuI RGATCY 1 cut(s) 964
PsyI GACNNNGTC 1 cut(s) 728
RsaI GTAC 2 cut(s) 267, 993
RsaNI GTAC 2 cut(s) 266, 992
SaqAI TTAA 3 cut(s) 299, 377, 884
SatI GCNGC 1 cut(s) 900
Sau3AI GATC 7 cut(s) 124, 283, 304, 338, 457, 505, 964
SfaNI GCATC 7 cut(s) 50, 65, 266, 572, 622, 731, 773
SfcI CTRYAG 2 cut(s) 816, 900
Sse9I AATT 4 cut(s) 225, 296, 300, 857
SsiI CCGC 1 cut(s) 794
SspI AATATT 1 cut(s) 909
SspMI CTAG 1 cut(s) 572
TaaI ACNGT 8 cut(s) 97, 109, 436, 499, 604, 616, 868, 1030
TaiI ACGT 1 cut(s) 1012
TasI AATT 4 cut(s) 225, 296, 300, 857
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 1 cut(s) 716
Tru1I TTAA 3 cut(s) 299, 377, 884
Tru9I TTAA 3 cut(s) 299, 377, 884
TscAI CASTG 3 cut(s) 112, 286, 502
TseFI GTSAC 4 cut(s) 31, 113, 277, 620
TseI GCWGC 1 cut(s) 899
Tsp45I GTSAC 4 cut(s) 31, 113, 277, 620
TspDTI ATGAA 6 cut(s) 40, 167, 456, 674, 746, 963
TspGWI ACGGA 4 cut(s) 101, 174, 345, 423
TspRI CASTG 3 cut(s) 112, 286, 502
Tth111I GACNNNGTC 1 cut(s) 728
VspI ATTAAT 1 cut(s) 299
XapI RAATTY 1 cut(s) 857
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.