Rh1AG029000

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003476
Physical Location & Seq
Forward (+)
5218092 .. 5246774
28683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG029000.1

Sequence Viewer

Length: 357 bp
ATGACGAAATGGATGGCAAAGATGGATGGCGAAGATGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGCCGAAGCTTCTTTTGACCCTGTCGTTGATGGGTGTGAAGGTGTTTTTCATACAGCATCCCCTGCCCTACTCTCATCTACTAATCCGCAGGCAGAATTAATTGAGCCTGCTTTGAAAGGAACGCTTAATGTCCTCGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTGGATTTAATGGAAAACCTCTTGCTGCTGATAGACTATCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

118

Amino Acids

12.62

Weight (kDa)

5.87

Isoelectric Point (pI)

32.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 9 - 107 6.9e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 290
AciI CCGC 1 cut(s) 200
AclWI GGATC 1 cut(s) 259
AgsI TTSAA 2 cut(s) 100, 229
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
AlwI GGATC 1 cut(s) 259
ApeKI GCWGC 2 cut(s) 305, 335
AseI ATTAAT 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 317, 322
BccI CCATC 5 cut(s) 7, 16, 20, 65, 137
BclI TGATCA 1 cut(s) 37
BisI GCNGC 2 cut(s) 306, 336
BlsI GCNGC 2 cut(s) 307, 337
BmsI GCATC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 4 cut(s) 18, 31, 170, 293
BseXI GCAGC 2 cut(s) 317, 322
Bsp143I GATC 2 cut(s) 37, 251
BspACI CCGC 1 cut(s) 200
BspPI GGATC 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 2 cut(s) 37, 251
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 2 cut(s) 204, 222
BstF5I GGATG 4 cut(s) 18, 31, 170, 293
BstKTI GATC 2 cut(s) 40, 254
BstMBI GATC 2 cut(s) 37, 251
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 317, 322
BtsCI GGATG 4 cut(s) 18, 31, 170, 293
Cac8I GCNNGC 2 cut(s) 204, 222
CviJI RGCY 4 cut(s) 88, 116, 122, 220
CviKI_1 RGCY 4 cut(s) 88, 116, 122, 220
DpnI GATC 2 cut(s) 39, 253
DpnII GATC 2 cut(s) 37, 251
FaiI YATR 3 cut(s) 165, 290, 302
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FbaI TGATCA 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 306, 336
FokI GGATG 4 cut(s) 25, 38, 157, 280
Fsp4HI GCNGC 2 cut(s) 306, 336
GluI GCNGC 2 cut(s) 306, 336
HindIII AAGCTT 1 cut(s) 120
Hpy188I TCNGA 1 cut(s) 251
HpyAV CCTTC 2 cut(s) 146, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 2 cut(s) 37, 251
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 4 cut(s) 147, 188, 189, 234
Lsp1109I GCAGC 2 cut(s) 317, 322
LweI GCATC 1 cut(s) 179
MalI GATC 2 cut(s) 39, 253
MboI GATC 2 cut(s) 37, 251
MboII GAAGA 1 cut(s) 44
MluCI AATT 2 cut(s) 209, 213
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 3 cut(s) 257, 307, 339
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 3 cut(s) 212, 240, 318
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeII GATC 2 cut(s) 37, 251
PdmI GAANNNNTTC 1 cut(s) 87
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 2 cut(s) 307, 337
PshBI ATTAAT 1 cut(s) 212
PsiI TTATAA 1 cut(s) 290
PsyI GACNNNGTC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 212, 240, 318
SatI GCNGC 2 cut(s) 306, 336
Sau3AI GATC 2 cut(s) 37, 251
SetI ASST 5 cut(s) 60, 124, 157, 267, 331
SfaNI GCATC 1 cut(s) 179
SgeI CNNG 9 cut(s) 80, 121, 146, 188, 215, 233, 260, 267, 344
Sse9I AATT 2 cut(s) 209, 213
SsiI CCGC 1 cut(s) 200
TasI AATT 2 cut(s) 209, 213
Tru1I TTAA 3 cut(s) 212, 240, 318
Tru9I TTAA 3 cut(s) 212, 240, 318
TseI GCWGC 2 cut(s) 305, 335
TspDTI ATGAA 2 cut(s) 80, 152
Tth111I GACNNNGTC 1 cut(s) 134
VspI ATTAAT 1 cut(s) 212
XmnI GAANNNNTTC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.