FvH4_7g25330

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001265
Physical Location & Seq
Reverse (-)
19368353 .. 19371071
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25330.t1

Sequence Viewer

Length: 1725 bp
ATGGAGGTTCTCAACAGCTCCATTTTCCTTTTAGGCGTTTGCCTTCTCTTGGCTTCATCGACAATGTCCTCCGCAGAACCCAAAACCCACAACCACGAATTTGTTATTCAAGCAACACCAGTGAAGAGGCTGTGCAAAACCCACAAAAGCATCACCGTGAACGGCCAGTTTCCCGGACCAACCTTGGAAGTTAACAACGGCGACACTCTCGTCGTCAAGGTCACGAACAAAGCTCGATATAACGTGACCATTCACTGGCATGGAATTCGCCAGATGAGGACTGGATGGGCAGATGGGCCGGAATTTGTAACTCAGTGCCCGATTAGACCTGGAGGGAGCTACACTTATCGGTTTACGATTCAAGGTCAAGAAGGAACACTGTGGTGGCATGCACACAGCTCTTGGCTTAGAGCCACTGTTTATGGAGCCCTTATTATTCATCCTAAACAAGGAGACTCGTATCCGTTCACTAAACCAAAGCGCGAGCAAACCCTTCTTCTGGGTGAGTGGTGGGATGCAAACCCTATCGACGTCCTGAGGCAGGCGACTCGAACCGGAGCTGCTCCGAATGTTTCTGATGCTTACACCATCAATGGTCAACCTGGTGATCTTTACAAGTGCTCGAGCAAAGACACAGTGATAGTTCCCATAAACTCCGGCGAGACCAACCTCCTCCGTGTCGTCAACGCCGCCCTTAACCAACAACTTTTCTTCGCCGTGGCCAACCATAAACTCACCGTTGTCAGTGCCGATGCCTCGTACACAAAGCCTTTCACCACGACCACCCTCATGCTCGGCCCCGGCCAAACCACTGACGTTTTAATAACCGGCGACCAGCCACCTTCCCGGTACTACATGGCTGCGCGTGCCTATTTCAGCGCACAAAACGCACAGTTCGACAACACCACCACCACCGCCATTCTAGAGTACGAGTCTGCTCCATGCAGCAACTGCAAAAACGGTCCATCAACTAAGCCCGTCATGCCGCAACTCCCAGCTTACAACGACACCAACACCGCCTCCGCCTTCACCACCAGCTTCCGAAGCCCTAGAAAAGTCGAAGTCCCCACCGATATCGACGAGAACCTCTTCTTCACCATCGGCCTCGGACTCAACAACTGCCCCAAGAACTTTCCCTCCAGTAGGTGCCAAGGCCCTAACGGTACGCGCTTCACCGCCAGCATGAACAATGTTTCTTTTGTGCTACCAAACAACATCTCCATCCTTCAAGCTTTCCAGCAGAACATCCCCGGGGTAGTAACTTCTGATTTCCCGGCAAACCCTCCGCAGCAGTTTGACTACACCGGCAACGTGAGCCGATCACTGTGGCAGCCCAAGTCCGGGACCAAAGGATACAGGTTAAATTACGGATCAAGGGTCCAGGTGGTGTTGCAAGACACTAGTATTGTCACACCCGAAAACCACCCGATTCATCTCCATGGATACGATTTCTACGTCCTTGCCGAAGGGTTTGGAAATTTCAATGCCAAGACTGACACGCAAAAATTCAACCTGGTTGATCCTCCTATGAGGAACACAGTTGCAGTACCAGTGAATGGTTGGGCCGTCATAAGATTCGTCGCTGACAATCCAGGTGCATGGATAATGCATTGTCACTTGGATGTTCACATCAACTGGGGTCTGGCTATGGTTTTCCTGGTGGATAACGGAGTCGGAAAGCTCCAATCAGTTGAGCCTCCACCGGCGGATCTTCCTCTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

575

Amino Acids

63.26

Weight (kDa)

8.23

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 150 2.1e-45 Multicopper oxidase
Cu-oxidase PF00394 162 - 312 7.8e-43 Multicopper oxidase
Cu-oxidase_2 PF07731 423 - 556 6e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 1 cut(s) 534
AccB1I GGYRCC 1 cut(s) 1146
AccB7I CCANNNNNTGG 2 cut(s) 255, 1556
AccII CGCG 3 cut(s) 483, 865, 1168
AciI CCGC 9 cut(s) 72, 690, 915, 986, 1017, 1023, 1176, 1286, 1706
AclWI GGATC 3 cut(s) 1378, 1514, 1716
AcoI YGGCCR 3 cut(s) 163, 720, 802
AcsI RAATTY 5 cut(s) 98, 264, 302, 1477, 1505
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 5 cut(s) 761, 851, 929, 1165, 1548
AfiI CCNNNNNNNGG 9 cut(s) 49, 255, 449, 499, 541, 1143, 1340, 1341, 1556
AgsI TTSAA 5 cut(s) 110, 362, 1229, 1483, 1510
AhlI ACTAGT 1 cut(s) 1400
AjnI CCWGG 6 cut(s) 328, 601, 1380, 1512, 1591, 1656
AleI CACNNNNGTG 1 cut(s) 382
AloI GAACNNNNNNTCC 2 cut(s) 1121, 1153
AluBI AGCT 9 cut(s) 18, 233, 339, 399, 560, 998, 1038, 1232, 1681
AluI AGCT 9 cut(s) 18, 233, 339, 399, 560, 998, 1038, 1232, 1681
Alw21I GWGCWC 1 cut(s) 623
Alw26I GTCTC 2 cut(s) 447, 656
AlwI GGATC 3 cut(s) 1378, 1514, 1716
AlwNI CAGNNNCTG 1 cut(s) 951
Ama87I CYCGRG 2 cut(s) 622, 1250
AoxI GGCC 8 cut(s) 163, 296, 720, 796, 802, 1102, 1153, 1563
ApeKI GCWGC 5 cut(s) 560, 860, 945, 1288, 1330
ApoI RAATTY 5 cut(s) 98, 264, 302, 1477, 1505
Asp700I GAANNNNTTC 1 cut(s) 1088
AspLEI GCGC 4 cut(s) 483, 865, 881, 1170
AspS9I GGNCC 8 cut(s) 176, 296, 797, 962, 1154, 1344, 1378, 1563
AsuC2I CCSGG 7 cut(s) 174, 801, 847, 1251, 1252, 1274, 1342
AsuHPI GGTGA 8 cut(s) 145, 515, 617, 727, 766, 1021, 1087, 1165
AvaI CYCGRG 2 cut(s) 622, 1250
AvaII GGWCC 4 cut(s) 176, 962, 1344, 1378
AxyI CCTNAGG 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 722
BanI GGYRCC 1 cut(s) 1146
BanII GRGCYC 1 cut(s) 430
Bbv12I GWGCWC 1 cut(s) 623
BbvI GCAGC 5 cut(s) 547, 847, 957, 1300, 1342
BccI CCATC 6 cut(s) 279, 287, 596, 973, 1106, 1229
BceAI ACGGC 4 cut(s) 178, 214, 701, 1550
BcgI CGANNNNNNTGC 4 cut(s) 248, 282, 530, 564
BciT130I CCWGG 6 cut(s) 330, 603, 1382, 1514, 1593, 1658
BciVI GTATCC 3 cut(s) 471, 1346, 1436
BcnI CCSGG 7 cut(s) 174, 801, 847, 1251, 1252, 1274, 1342
BcoDI GTCTC 2 cut(s) 447, 656
BcuI ACTAGT 1 cut(s) 1400
BfaI CTAG 3 cut(s) 923, 1050, 1401
BfuI GTATCC 3 cut(s) 471, 1346, 1436
BisI GCNGC 7 cut(s) 561, 690, 861, 946, 986, 1289, 1331
BlsI GCNGC 7 cut(s) 562, 691, 862, 947, 987, 1290, 1332
Bme18I GGWCC 4 cut(s) 176, 962, 1344, 1378
BmeT110I CYCGRG 2 cut(s) 622, 1250
BmgT120I GGNCC 8 cut(s) 176, 296, 797, 962, 1154, 1344, 1378, 1563
BmiI GGNNCC 5 cut(s) 427, 799, 1148, 1345, 1379
BmrI ACTGGG 1 cut(s) 1645
BmsI GCATC 4 cut(s) 159, 505, 568, 742
BmuI ACTGGG 1 cut(s) 1645
BpmI CTGGAG 2 cut(s) 351, 1123
BpuMI CCSGG 7 cut(s) 174, 801, 847, 1251, 1252, 1274, 1342
BsaHI GRCGYC 1 cut(s) 531
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 9 cut(s) 183, 717, 799, 1105, 1150, 1249, 1250, 1251, 1438
BsaWI WCCGGW 1 cut(s) 554
BsaXI ACNNNNNCTCC 6 cut(s) 1004, 1034, 1121, 1151, 1202, 1232
Bsc4I CCNNNNNNNGG 9 cut(s) 49, 255, 449, 499, 541, 1143, 1340, 1341, 1556
Bse118I RCCGGY 3 cut(s) 827, 1304, 1702
Bse1I ACTGG 7 cut(s) 119, 166, 260, 286, 1140, 1550, 1640
Bse21I CCTNAGG 1 cut(s) 536
BseBI CCWGG 6 cut(s) 330, 603, 1382, 1514, 1593, 1658
BseDI CCNNGG 9 cut(s) 183, 717, 799, 1105, 1150, 1249, 1250, 1251, 1438
BseGI GGATG 6 cut(s) 290, 439, 520, 1221, 1245, 1627
BseLI CCNNNNNNNGG 9 cut(s) 49, 255, 449, 499, 541, 1143, 1340, 1341, 1556
BseMII CTCAG 2 cut(s) 326, 527
BseNI ACTGG 7 cut(s) 119, 166, 260, 286, 1140, 1550, 1640
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 5 cut(s) 547, 847, 957, 1300, 1342
BseYI CCCAGC 1 cut(s) 994
Bsh1236I CGCG 3 cut(s) 483, 865, 1168
BshFI GGCC 8 cut(s) 165, 298, 722, 798, 804, 1104, 1155, 1565
BshNI GGYRCC 1 cut(s) 1146
BsiHKAI GWGCWC 1 cut(s) 623
BsiHKCI CYCGRG 2 cut(s) 622, 1250
BslFI GGGAC 2 cut(s) 1049, 1357
BslI CCNNNNNNNGG 9 cut(s) 49, 255, 449, 499, 541, 1143, 1340, 1341, 1556
BsmAI GTCTC 2 cut(s) 447, 656
BsmFI GGGAC 2 cut(s) 1049, 1357
BsnI GGCC 8 cut(s) 165, 298, 722, 798, 804, 1104, 1155, 1565
Bso31I GGTCTC 1 cut(s) 656
BsoBI CYCGRG 2 cut(s) 622, 1250
Bsp1286I GDGCHC 3 cut(s) 320, 430, 623
Bsp143I GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
Bsp19I CCATGG 1 cut(s) 1438
BspACI CCGC 9 cut(s) 72, 690, 915, 986, 1017, 1023, 1176, 1286, 1706
BspANI GGCC 8 cut(s) 165, 298, 722, 798, 804, 1104, 1155, 1565
BspCNI CTCAG 2 cut(s) 325, 528
BspFNI CGCG 3 cut(s) 483, 865, 1168
BspLI GGNNCC 5 cut(s) 427, 799, 1148, 1345, 1379
BspPI GGATC 3 cut(s) 1378, 1514, 1716
BspT107I GGYRCC 1 cut(s) 1146
BspTNI GGTCTC 1 cut(s) 656
BsrFI RCCGGY 3 cut(s) 827, 1304, 1702
BsrI ACTGG 7 cut(s) 119, 166, 260, 286, 1140, 1550, 1640
BssAI RCCGGY 3 cut(s) 827, 1304, 1702
BssECI CCNNGG 9 cut(s) 183, 717, 799, 1105, 1150, 1249, 1250, 1251, 1438
BssMI GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
BssNI GRCGYC 1 cut(s) 531
BssT1I CCWWGG 3 cut(s) 183, 1150, 1438
Bst2UI CCWGG 6 cut(s) 330, 603, 1382, 1514, 1593, 1658
Bst6I CTCTTC 2 cut(s) 119, 1094
BstACI GRCGYC 1 cut(s) 531
BstAPI GCANNNNNTGC 1 cut(s) 951
BstC8I GCNNGC 5 cut(s) 390, 485, 543, 867, 1180
BstDEI CTNAG 4 cut(s) 312, 407, 536, 972
BstDSI CCRYGG 2 cut(s) 717, 1438
BstENI CCTNNNNNAGG 3 cut(s) 447, 539, 1141
BstF5I GGATG 6 cut(s) 290, 439, 520, 1221, 1245, 1627
BstFNI CGCG 3 cut(s) 483, 865, 1168
BstHHI GCGC 4 cut(s) 483, 865, 881, 1170
BstKTI GATC 5 cut(s) 610, 1322, 1373, 1522, 1711
BstMAI GTCTC 2 cut(s) 447, 656
BstMBI GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
BstMWI GCNNNNNNNGC 6 cut(s) 866, 887, 951, 982, 1044, 1314
BstNI CCWGG 6 cut(s) 330, 603, 1382, 1514, 1593, 1658
BstNSI RCATGY 1 cut(s) 392
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 3 cut(s) 483, 865, 1168
BstV1I GCAGC 5 cut(s) 547, 847, 957, 1300, 1342
BstX2I RGATCY 1 cut(s) 1708
BstXI CCANNNNNNTGG 1 cut(s) 1599
BstYI RGATCY 1 cut(s) 1708
Bsu36I CCTNAGG 1 cut(s) 536
BsuI GTATCC 3 cut(s) 471, 1346, 1436
BsuRI GGCC 8 cut(s) 165, 298, 722, 798, 804, 1104, 1155, 1565
BtgI CCRYGG 2 cut(s) 717, 1438
BtsCI GGATG 6 cut(s) 290, 439, 520, 1221, 1245, 1627
Cac8I GCNNGC 5 cut(s) 390, 485, 543, 867, 1180
CaiI CAGNNNCTG 1 cut(s) 951
CfoI GCGC 4 cut(s) 483, 865, 881, 1170
Cfr10I RCCGGY 3 cut(s) 827, 1304, 1702
Cfr13I GGNCC 8 cut(s) 176, 296, 797, 962, 1154, 1344, 1378, 1563
Cfr9I CCCGGG 1 cut(s) 1250
CsiI ACCWGGT 2 cut(s) 601, 1512
Csp6I GTAC 5 cut(s) 760, 850, 928, 1164, 1547
CviAII CATG 9 cut(s) 260, 389, 790, 856, 942, 982, 1183, 1439, 1599
CviQI GTAC 5 cut(s) 760, 850, 928, 1164, 1547
DdeI CTNAG 4 cut(s) 312, 407, 536, 972
DpnI GATC 5 cut(s) 609, 1321, 1372, 1521, 1710
DpnII GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 3 cut(s) 163, 720, 802
Eam1104I CTCTTC 2 cut(s) 119, 1094
EarI CTCTTC 2 cut(s) 119, 1094
EciI GGCGGA 2 cut(s) 1012, 1721
Eco130I CCWWGG 3 cut(s) 183, 1150, 1438
Eco24I GRGCYC 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 656
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 4 cut(s) 176, 962, 1344, 1378
Eco81I CCTNAGG 1 cut(s) 536
Eco88I CYCGRG 2 cut(s) 622, 1250
EcoNI CCTNNNNNAGG 3 cut(s) 447, 539, 1141
EcoO109I RGGNCCY 1 cut(s) 1154
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 6 cut(s) 328, 601, 1380, 1512, 1591, 1656
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 3 cut(s) 183, 1150, 1438
EcoT22I ATGCAT 1 cut(s) 1611
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 3 cut(s) 183, 1150, 1438
FaeI CATG 9 cut(s) 263, 392, 793, 859, 945, 985, 1186, 1442, 1602
FaqI GGGAC 2 cut(s) 1049, 1357
FatI CATG 9 cut(s) 259, 388, 789, 855, 941, 981, 1182, 1438, 1598
Fnu4HI GCNGC 7 cut(s) 561, 690, 861, 946, 986, 1289, 1331
FokI GGATG 6 cut(s) 297, 426, 527, 1208, 1232, 1634
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 7 cut(s) 561, 690, 861, 946, 986, 1289, 1331
FspBI CTAG 3 cut(s) 923, 1050, 1401
GlaI GCGC 4 cut(s) 482, 864, 880, 1169
GluI GCNGC 7 cut(s) 561, 690, 861, 946, 986, 1289, 1331
GsaI CCCAGC 1 cut(s) 998
GsuI CTGGAG 2 cut(s) 351, 1123
HaeIII GGCC 8 cut(s) 165, 298, 722, 798, 804, 1104, 1155, 1565
HhaI GCGC 4 cut(s) 483, 865, 881, 1170
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 9 cut(s) 263, 392, 793, 859, 945, 985, 1186, 1442, 1602
Hin6I GCGC 4 cut(s) 481, 863, 879, 1168
HinP1I GCGC 4 cut(s) 481, 863, 879, 1168
HincII GTYRAC 3 cut(s) 193, 599, 685
HindII GTYRAC 3 cut(s) 193, 599, 685
HindIII AAGCTT 1 cut(s) 1230
HinfI GANTC 8 cut(s) 358, 455, 547, 932, 1110, 1429, 1575, 1671
HpaI GTTAAC 1 cut(s) 193
HphI GGTGA 8 cut(s) 145, 515, 617, 727, 766, 1021, 1087, 1165
Hpy166II GTNNAC 8 cut(s) 160, 193, 354, 468, 599, 685, 762, 1627
Hpy188I TCNGA 6 cut(s) 567, 577, 1043, 1109, 1267, 1676
Hpy188III TCNNGA 4 cut(s) 223, 368, 535, 923
Hpy8I GTNNAC 8 cut(s) 160, 193, 354, 468, 599, 685, 762, 1627
Hpy99I CGWCG 4 cut(s) 215, 533, 1082, 1583
HpyAV CCTTC 7 cut(s) 53, 365, 503, 852, 1036, 1235, 1460
HpyCH4IV ACGT 5 cut(s) 243, 531, 816, 1311, 1455
HpyCH4V TGCA 9 cut(s) 135, 392, 518, 945, 954, 1393, 1544, 1598, 1609
HpyF10VI GCNNNNNNNGC 6 cut(s) 866, 887, 951, 982, 1044, 1314
HpyF3I CTNAG 4 cut(s) 312, 407, 536, 972
HpySE526I ACGT 5 cut(s) 243, 531, 816, 1311, 1455
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 9 cut(s) 263, 392, 793, 859, 945, 985, 1186, 1442, 1602
HspAI GCGC 4 cut(s) 481, 863, 879, 1168
KspAI GTTAAC 1 cut(s) 193
Kzo9I GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
LmnI GCTCC 7 cut(s) 23, 336, 425, 557, 568, 943, 1686
Lsp1109I GCAGC 5 cut(s) 547, 847, 957, 1300, 1342
LweI GCATC 4 cut(s) 159, 505, 568, 742
MabI ACCWGGT 2 cut(s) 601, 1512
MaeI CTAG 3 cut(s) 923, 1050, 1401
MaeII ACGT 5 cut(s) 243, 531, 816, 1311, 1455
MaeIII GTNAC 6 cut(s) 220, 244, 307, 1258, 1408, 1613
MalI GATC 5 cut(s) 609, 1321, 1372, 1521, 1710
MboI GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
MboII GAAGA 6 cut(s) 136, 488, 703, 1081, 1084, 1703
MflI RGATCY 1 cut(s) 1708
MhlI GDGCHC 3 cut(s) 320, 430, 623
MlsI TGGCCA 1 cut(s) 722
MluCI AATT 6 cut(s) 98, 264, 302, 1363, 1477, 1505
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 5 cut(s) 449, 541, 941, 1104, 1680
MmeI TCCRAC 1 cut(s) 1654
Mox20I TGGCCA 1 cut(s) 722
Mph1103I ATGCAT 1 cut(s) 1611
MroXI GAANNNNTTC 1 cut(s) 1088
MscI TGGCCA 1 cut(s) 722
MseI TTAA 5 cut(s) 192, 696, 821, 1361, 1723
MslI CAYNNNNRTG 6 cut(s) 155, 258, 382, 788, 1437, 1620
Msp20I TGGCCA 1 cut(s) 722
MvaI CCWGG 6 cut(s) 330, 603, 1382, 1514, 1593, 1658
MvnI CGCG 3 cut(s) 483, 865, 1168
MwoI GCNNNNNNNGC 6 cut(s) 866, 887, 951, 982, 1044, 1314
NciI CCSGG 7 cut(s) 174, 801, 847, 1251, 1252, 1274, 1342
NcoI CCATGG 1 cut(s) 1438
NdeII GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
NlaIII CATG 9 cut(s) 263, 392, 793, 859, 945, 985, 1186, 1442, 1602
NlaIV GGNNCC 5 cut(s) 427, 799, 1148, 1345, 1379
NmeAIII GCCGAG 1 cut(s) 774
NmuCI GTSAC 4 cut(s) 220, 244, 1408, 1613
NsiI ATGCAT 1 cut(s) 1611
NspI RCATGY 1 cut(s) 392
OliI CACNNNNGTG 1 cut(s) 382
PaeI GCATGC 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 622
PcsI WCGNNNNNNNCGW 3 cut(s) 894, 1452, 1461
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 3 cut(s) 358, 1429, 1575
PflFI GACNNNGTC 1 cut(s) 64
PflMI CCANNNNNTGG 2 cut(s) 255, 1556
PfoI TCCNGGA 2 cut(s) 172, 1340
PkrI GCNGC 7 cut(s) 562, 691, 862, 947, 987, 1290, 1332
PleI GAGTC 5 cut(s) 449, 541, 940, 1104, 1679
PpsI GAGTC 5 cut(s) 449, 541, 940, 1104, 1679
Psp6I CCWGG 6 cut(s) 328, 601, 1380, 1512, 1591, 1656
PspFI CCCAGC 1 cut(s) 994
PspGI CCWGG 6 cut(s) 328, 601, 1380, 1512, 1591, 1656
PspN4I GGNNCC 5 cut(s) 427, 799, 1148, 1345, 1379
PspPI GGNCC 8 cut(s) 176, 296, 797, 962, 1154, 1344, 1378, 1563
PspXI VCTCGAGB 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 951
PsuI RGATCY 1 cut(s) 1708
PsyI GACNNNGTC 1 cut(s) 64
RsaI GTAC 5 cut(s) 761, 851, 929, 1165, 1548
RsaNI GTAC 5 cut(s) 760, 850, 928, 1164, 1547
RseI CAYNNNNRTG 6 cut(s) 155, 258, 382, 788, 1437, 1620
SaqAI TTAA 5 cut(s) 192, 696, 821, 1361, 1723
SatI GCNGC 7 cut(s) 561, 690, 861, 946, 986, 1289, 1331
Sau3AI GATC 5 cut(s) 607, 1319, 1370, 1519, 1708
Sau96I GGNCC 8 cut(s) 176, 296, 797, 962, 1154, 1344, 1378, 1563
SchI GAGTC 5 cut(s) 449, 541, 941, 1104, 1680
SduI GDGCHC 3 cut(s) 320, 430, 623
SexAI ACCWGGT 2 cut(s) 601, 1512
SfaNI GCATC 4 cut(s) 159, 505, 568, 742
Sfr274I CTCGAG 1 cut(s) 622
SgrAI CRCCGGYG 1 cut(s) 1702
SinI GGWCC 4 cut(s) 176, 962, 1344, 1378
SlaI CTCGAG 1 cut(s) 622
SmaI CCCGGG 1 cut(s) 1252
SmiMI CAYNNNNRTG 6 cut(s) 155, 258, 382, 788, 1437, 1620
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
SpeI ACTAGT 1 cut(s) 1400
SphI GCATGC 1 cut(s) 392
Sse9I AATT 6 cut(s) 98, 264, 302, 1363, 1477, 1505
SsiI CCGC 9 cut(s) 72, 690, 915, 986, 1017, 1023, 1176, 1286, 1706
SspMI CTAG 3 cut(s) 923, 1050, 1401
StyI CCWWGG 3 cut(s) 183, 1150, 1438
TaiI ACGT 5 cut(s) 246, 534, 819, 1314, 1458
TaqI TCGA 8 cut(s) 59, 235, 528, 550, 623, 897, 1059, 1077
TasI AATT 6 cut(s) 98, 264, 302, 1363, 1477, 1505
TauI GCSGC 2 cut(s) 692, 988
TfiI GAWTC 3 cut(s) 358, 1429, 1575
Tru1I TTAA 5 cut(s) 192, 696, 821, 1361, 1723
Tru9I TTAA 5 cut(s) 192, 696, 821, 1361, 1723
TseFI GTSAC 4 cut(s) 220, 244, 1408, 1613
TseI GCWGC 5 cut(s) 560, 860, 945, 1288, 1330
Tsp45I GTSAC 4 cut(s) 220, 244, 1408, 1613
TspDTI ATGAA 4 cut(s) 45, 428, 1199, 1421
TspGWI ACGGA 4 cut(s) 453, 665, 1383, 1683
TspMI CCCGGG 1 cut(s) 1250
Tth111I GACNNNGTC 1 cut(s) 64
Van91I CCANNNNNTGG 2 cut(s) 255, 1556
VpaK11BI GGWCC 4 cut(s) 176, 962, 1344, 1378
XagI CCTNNNNNAGG 3 cut(s) 447, 539, 1141
XapI RAATTY 5 cut(s) 98, 264, 302, 1477, 1505
XbaI TCTAGA 1 cut(s) 922
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 2 cut(s) 278, 1557
XhoI CTCGAG 1 cut(s) 622
XmaI CCCGGG 1 cut(s) 1250
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 3 cut(s) 923, 1050, 1401
ZraI GACGTC 1 cut(s) 532
Zsp2I ATGCAT 1 cut(s) 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.