Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 161 |
| Acc65I | GGTACC | 1 cut(s) | 1498 |
| AccB1I | GGYRCC | 3 cut(s) | 698, 1098, 1498 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1508 |
| AccBSI | CCGCTC | 1 cut(s) | 644 |
| AccII | CGCG | 4 cut(s) | 435, 817, 1120, 1240 |
| AciI | CCGC | 9 cut(s) | 642, 867, 975, 1128, 1227, 1235, 1238, 1240, 1496 |
| AclWI | GGATC | 3 cut(s) | 1330, 1466, 1668 |
| AcsI | RAATTY | 5 cut(s) | 50, 216, 254, 1081, 1457 |
| AcyI | GRCGYC | 1 cut(s) | 705 |
| AfaI | GTAC | 4 cut(s) | 328, 803, 881, 1500 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 401, 1006, 1095, 1292, 1293, 1508 |
| AgeI | ACCGGT | 1 cut(s) | 300 |
| AgsI | TTSAA | 5 cut(s) | 62, 1181, 1315, 1435, 1462 |
| AhlI | ACTAGT | 1 cut(s) | 1352 |
| AjiI | CACGTC | 1 cut(s) | 484 |
| AjnI | CCWGG | 5 cut(s) | 553, 1332, 1464, 1543, 1608 |
| AleI | CACNNNNGTG | 1 cut(s) | 334 |
| AluBI | AGCT | 7 cut(s) | 185, 291, 351, 515, 990, 1184, 1633 |
| AluI | AGCT | 7 cut(s) | 185, 291, 351, 515, 990, 1184, 1633 |
| Alw21I | GWGCWC | 1 cut(s) | 382 |
| Alw26I | GTCTC | 4 cut(s) | 399, 431, 1150, 1653 |
| AlwI | GGATC | 3 cut(s) | 1330, 1466, 1668 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 903 |
| Ama87I | CYCGRG | 3 cut(s) | 574, 752, 875 |
| AoxI | GGCC | 5 cut(s) | 248, 278, 748, 1054, 1105 |
| ApeKI | GCWGC | 5 cut(s) | 512, 812, 842, 897, 1282 |
| ApoI | RAATTY | 5 cut(s) | 50, 216, 254, 1081, 1457 |
| AsiGI | ACCGGT | 1 cut(s) | 300 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1040 |
| Asp718I | GGTACC | 1 cut(s) | 1498 |
| AspLEI | GCGC | 4 cut(s) | 435, 817, 833, 1122 |
| AspS9I | GGNCC | 6 cut(s) | 128, 248, 279, 749, 1106, 1296 |
| AsuC2I | CCSGG | 6 cut(s) | 126, 282, 753, 754, 1203, 1294 |
| AvaI | CYCGRG | 3 cut(s) | 574, 752, 875 |
| AvaII | GGWCC | 2 cut(s) | 128, 1296 |
| BaeGI | GKGCMC | 1 cut(s) | 272 |
| BanI | GGYRCC | 3 cut(s) | 698, 1098, 1498 |
| Bbv12I | GWGCWC | 1 cut(s) | 382 |
| BbvI | GCAGC | 5 cut(s) | 524, 824, 854, 909, 1294 |
| BccI | CCATC | 5 cut(s) | 231, 239, 485, 802, 1181 |
| BceAI | ACGGC | 2 cut(s) | 928, 1129 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 200, 234 |
| BciT130I | CCWGG | 5 cut(s) | 555, 1334, 1466, 1545, 1610 |
| BciVI | GTATCC | 3 cut(s) | 423, 1298, 1388 |
| BcnI | CCSGG | 6 cut(s) | 126, 282, 753, 754, 1203, 1294 |
| BcoDI | GTCTC | 4 cut(s) | 399, 431, 1150, 1653 |
| BcuI | ACTAGT | 1 cut(s) | 1352 |
| BfaI | CTAG | 3 cut(s) | 161, 1002, 1353 |
| BfuI | GTATCC | 3 cut(s) | 423, 1298, 1388 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1113 |
| BisI | GCNGC | 8 cut(s) | 513, 642, 813, 843, 898, 1238, 1241, 1283 |
| BlsI | GCNGC | 8 cut(s) | 514, 643, 814, 844, 899, 1239, 1242, 1284 |
| Bme18I | GGWCC | 2 cut(s) | 128, 1296 |
| BmeT110I | CYCGRG | 3 cut(s) | 574, 752, 875 |
| BmgBI | CACGTC | 1 cut(s) | 484 |
| BmgT120I | GGNCC | 6 cut(s) | 128, 248, 279, 749, 1106, 1296 |
| BmiI | GGNNCC | 5 cut(s) | 700, 751, 1100, 1297, 1500 |
| BmrI | ACTGGG | 1 cut(s) | 1597 |
| BmsI | GCATC | 2 cut(s) | 111, 520 |
| BmuI | ACTGGG | 1 cut(s) | 1597 |
| BpmI | CTGGAG | 2 cut(s) | 1075, 1619 |
| BpuMI | CCSGG | 6 cut(s) | 126, 282, 753, 754, 1203, 1294 |
| BsaHI | GRCGYC | 1 cut(s) | 705 |
| BsaWI | WCCGGW | 2 cut(s) | 300, 506 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 956, 986, 1154, 1184 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 401, 1006, 1095, 1292, 1293, 1508 |
| Bse118I | RCCGGY | 3 cut(s) | 300, 779, 1256 |
| Bse1I | ACTGG | 7 cut(s) | 71, 212, 238, 1092, 1502, 1592, 1636 |
| BseBI | CCWGG | 5 cut(s) | 555, 1334, 1466, 1545, 1610 |
| BseGI | GGATG | 4 cut(s) | 242, 391, 1173, 1579 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 401, 1006, 1095, 1292, 1293, 1508 |
| BseMII | CTCAG | 2 cut(s) | 107, 278 |
| BseNI | ACTGG | 7 cut(s) | 71, 212, 238, 1092, 1502, 1592, 1636 |
| BseRI | GAGGAG | 2 cut(s) | 614, 961 |
| BseSI | GKGCMC | 1 cut(s) | 272 |
| BseXI | GCAGC | 5 cut(s) | 524, 824, 854, 909, 1294 |
| Bsh1236I | CGCG | 4 cut(s) | 435, 817, 1120, 1240 |
| BshFI | GGCC | 5 cut(s) | 250, 280, 750, 1056, 1107 |
| BshNI | GGYRCC | 3 cut(s) | 698, 1098, 1498 |
| BshTI | ACCGGT | 1 cut(s) | 300 |
| BsiHKAI | GWGCWC | 1 cut(s) | 382 |
| BsiHKCI | CYCGRG | 3 cut(s) | 574, 752, 875 |
| BslFI | GGGAC | 2 cut(s) | 1001, 1309 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 401, 1006, 1095, 1292, 1293, 1508 |
| BsmAI | GTCTC | 4 cut(s) | 399, 431, 1150, 1653 |
| BsmFI | GGGAC | 2 cut(s) | 1001, 1309 |
| BsmI | GAATGC | 2 cut(s) | 472, 845 |
| BsnI | GGCC | 5 cut(s) | 250, 280, 750, 1056, 1107 |
| BsoBI | CYCGRG | 3 cut(s) | 574, 752, 875 |
| Bsp1286I | GDGCHC | 2 cut(s) | 272, 382 |
| Bsp143I | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| BspACI | CCGC | 9 cut(s) | 642, 867, 975, 1128, 1227, 1235, 1238, 1240, 1496 |
| BspANI | GGCC | 5 cut(s) | 250, 280, 750, 1056, 1107 |
| BspCNI | CTCAG | 2 cut(s) | 106, 277 |
| BspFNI | CGCG | 4 cut(s) | 435, 817, 1120, 1240 |
| BspLI | GGNNCC | 5 cut(s) | 700, 751, 1100, 1297, 1500 |
| BspPI | GGATC | 3 cut(s) | 1330, 1466, 1668 |
| BspT107I | GGYRCC | 3 cut(s) | 698, 1098, 1498 |
| BsrBI | CCGCTC | 1 cut(s) | 644 |
| BsrFI | RCCGGY | 3 cut(s) | 300, 779, 1256 |
| BsrI | ACTGG | 7 cut(s) | 71, 212, 238, 1092, 1502, 1592, 1636 |
| BssAI | RCCGGY | 3 cut(s) | 300, 779, 1256 |
| BssMI | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| BssNI | GRCGYC | 1 cut(s) | 705 |
| BssT1I | CCWWGG | 4 cut(s) | 135, 313, 1102, 1299 |
| Bst2UI | CCWGG | 5 cut(s) | 555, 1334, 1466, 1545, 1610 |
| Bst4CI | ACNGT | 9 cut(s) | 109, 120, 370, 589, 670, 691, 1051, 1492, 1656 |
| Bst6I | CTCTTC | 2 cut(s) | 71, 1046 |
| BstACI | GRCGYC | 1 cut(s) | 705 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 818, 839, 903 |
| BstC8I | GCNNGC | 4 cut(s) | 342, 819, 1118, 1132 |
| BstDEI | CTNAG | 4 cut(s) | 93, 264, 359, 924 |
| BstDSI | CCRYGG | 2 cut(s) | 1238, 1390 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 399, 1093 |
| BstF5I | GGATG | 4 cut(s) | 242, 391, 1173, 1579 |
| BstFNI | CGCG | 4 cut(s) | 435, 817, 1120, 1240 |
| BstHHI | GCGC | 4 cut(s) | 435, 817, 833, 1122 |
| BstKTI | GATC | 4 cut(s) | 562, 1325, 1474, 1663 |
| BstMAI | GTCTC | 4 cut(s) | 399, 431, 1150, 1653 |
| BstMBI | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 818, 839, 903, 912, 934, 996, 1113, 1266 |
| BstNI | CCWGG | 5 cut(s) | 555, 1334, 1466, 1545, 1610 |
| BstSLI | GKGCMC | 1 cut(s) | 272 |
| BstUI | CGCG | 4 cut(s) | 435, 817, 1120, 1240 |
| BstV1I | GCAGC | 5 cut(s) | 524, 824, 854, 909, 1294 |
| BstX2I | RGATCY | 1 cut(s) | 1660 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 484, 1551 |
| BstYI | RGATCY | 1 cut(s) | 1660 |
| BsuI | GTATCC | 3 cut(s) | 423, 1298, 1388 |
| BsuRI | GGCC | 5 cut(s) | 250, 280, 750, 1056, 1107 |
| BtgI | CCRYGG | 2 cut(s) | 1238, 1390 |
| BtrI | CACGTC | 1 cut(s) | 484 |
| BtsCI | GGATG | 4 cut(s) | 242, 391, 1173, 1579 |
| BtsIMutI | CAGTG | 5 cut(s) | 78, 205, 272, 594, 1509 |
| Cac8I | GCNNGC | 4 cut(s) | 342, 819, 1118, 1132 |
| CaiI | CAGNNNCTG | 1 cut(s) | 903 |
| CfoI | GCGC | 4 cut(s) | 435, 817, 833, 1122 |
| Cfr10I | RCCGGY | 3 cut(s) | 300, 779, 1256 |
| Cfr13I | GGNCC | 6 cut(s) | 128, 248, 279, 749, 1106, 1296 |
| Cfr42I | CCGCGG | 1 cut(s) | 1241 |
| Cfr9I | CCCGGG | 1 cut(s) | 752 |
| CseI | GACGC | 1 cut(s) | 713 |
| CsiI | ACCWGGT | 2 cut(s) | 553, 1464 |
| Csp6I | GTAC | 4 cut(s) | 327, 802, 880, 1499 |
| CspAI | ACCGGT | 1 cut(s) | 300 |
| CviAII | CATG | 7 cut(s) | 47, 212, 742, 808, 894, 934, 1135 |
| CviQI | GTAC | 4 cut(s) | 327, 802, 880, 1499 |
| DdeI | CTNAG | 4 cut(s) | 93, 264, 359, 924 |
| DpnI | GATC | 4 cut(s) | 561, 1324, 1473, 1662 |
| DpnII | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 161 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 161 |
| Eam1104I | CTCTTC | 2 cut(s) | 71, 1046 |
| EarI | CTCTTC | 2 cut(s) | 71, 1046 |
| EciI | GGCGGA | 1 cut(s) | 964 |
| Eco130I | CCWWGG | 4 cut(s) | 135, 313, 1102, 1299 |
| Eco47I | GGWCC | 2 cut(s) | 128, 1296 |
| Eco88I | CYCGRG | 3 cut(s) | 574, 752, 875 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 399, 1093 |
| EcoO109I | RGGNCCY | 2 cut(s) | 749, 1106 |
| EcoRI | GAATTC | 1 cut(s) | 216 |
| EcoRII | CCWGG | 5 cut(s) | 553, 1332, 1464, 1543, 1608 |
| EcoT14I | CCWWGG | 4 cut(s) | 135, 313, 1102, 1299 |
| EcoT22I | ATGCAT | 1 cut(s) | 1563 |
| ErhI | CCWWGG | 4 cut(s) | 135, 313, 1102, 1299 |
| FaeI | CATG | 7 cut(s) | 50, 215, 745, 811, 897, 937, 1138 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 280, 312 |
| FaqI | GGGAC | 2 cut(s) | 1001, 1309 |
| FatI | CATG | 7 cut(s) | 46, 211, 741, 807, 893, 933, 1134 |
| FauI | CCCGC | 2 cut(s) | 1234, 1242 |
| Fnu4HI | GCNGC | 8 cut(s) | 513, 642, 813, 843, 898, 1238, 1241, 1283 |
| FokI | GGATG | 4 cut(s) | 249, 378, 1160, 1586 |
| Fsp4HI | GCNGC | 8 cut(s) | 513, 642, 813, 843, 898, 1238, 1241, 1283 |
| FspBI | CTAG | 3 cut(s) | 161, 1002, 1353 |
| GlaI | GCGC | 4 cut(s) | 434, 816, 832, 1121 |
| GluI | GCNGC | 8 cut(s) | 513, 642, 813, 843, 898, 1238, 1241, 1283 |
| GsuI | CTGGAG | 2 cut(s) | 1075, 1619 |
| HaeIII | GGCC | 5 cut(s) | 250, 280, 750, 1056, 1107 |
| HgaI | GACGC | 1 cut(s) | 713 |
| HhaI | GCGC | 4 cut(s) | 435, 817, 833, 1122 |
| Hin1I | GRCGYC | 1 cut(s) | 705 |
| Hin1II | CATG | 7 cut(s) | 50, 215, 745, 811, 897, 937, 1138 |
| Hin6I | GCGC | 4 cut(s) | 433, 815, 831, 1120 |
| HinP1I | GCGC | 4 cut(s) | 433, 815, 831, 1120 |
| HincII | GTYRAC | 2 cut(s) | 145, 551 |
| HindII | GTYRAC | 2 cut(s) | 145, 551 |
| HindIII | AAGCTT | 1 cut(s) | 1182 |
| HinfI | GANTC | 8 cut(s) | 407, 605, 630, 1062, 1329, 1381, 1623, 1646 |
| HpaI | GTTAAC | 1 cut(s) | 145 |
| Hpy166II | GTNNAC | 6 cut(s) | 112, 145, 306, 329, 551, 1579 |
| Hpy188I | TCNGA | 7 cut(s) | 96, 529, 629, 995, 1061, 1219, 1526 |
| Hpy188III | TCNNGA | 5 cut(s) | 175, 320, 875, 1326, 1627 |
| Hpy8I | GTNNAC | 6 cut(s) | 112, 145, 306, 329, 551, 1579 |
| Hpy99I | CGWCG | 5 cut(s) | 485, 674, 707, 770, 1034 |
| HpyAV | CCTTC | 5 cut(s) | 317, 733, 988, 1187, 1412 |
| HpyCH4III | ACNGT | 9 cut(s) | 109, 120, 370, 589, 670, 691, 1051, 1492, 1656 |
| HpyCH4IV | ACGT | 5 cut(s) | 195, 483, 768, 1263, 1407 |
| HpyCH4V | TGCA | 8 cut(s) | 87, 470, 842, 897, 906, 1345, 1415, 1561 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 818, 839, 903, 912, 934, 996, 1113, 1266 |
| HpyF3I | CTNAG | 4 cut(s) | 93, 264, 359, 924 |
| HpySE526I | ACGT | 5 cut(s) | 195, 483, 768, 1263, 1407 |
| Hsp92I | GRCGYC | 1 cut(s) | 705 |
| Hsp92II | CATG | 7 cut(s) | 50, 215, 745, 811, 897, 937, 1138 |
| HspAI | GCGC | 4 cut(s) | 433, 815, 831, 1120 |
| KpnI | GGTACC | 1 cut(s) | 1502 |
| KspAI | GTTAAC | 1 cut(s) | 145 |
| KspI | CCGCGG | 1 cut(s) | 1241 |
| Kzo9I | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| LmnI | GCTCC | 5 cut(s) | 377, 509, 520, 895, 1638 |
| Lsp1109I | GCAGC | 5 cut(s) | 524, 824, 854, 909, 1294 |
| LweI | GCATC | 2 cut(s) | 111, 520 |
| MabI | ACCWGGT | 2 cut(s) | 553, 1464 |
| MaeI | CTAG | 3 cut(s) | 161, 1002, 1353 |
| MaeII | ACGT | 5 cut(s) | 195, 483, 768, 1263, 1407 |
| MaeIII | GTNAC | 7 cut(s) | 120, 172, 196, 259, 1210, 1360, 1565 |
| MalI | GATC | 4 cut(s) | 561, 1324, 1473, 1662 |
| MbiI | CCGCTC | 1 cut(s) | 644 |
| MboI | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| MboII | GAAGA | 6 cut(s) | 88, 440, 655, 1033, 1036, 1655 |
| MfeI | CAATTG | 1 cut(s) | 568 |
| MflI | RGATCY | 1 cut(s) | 1660 |
| MhlI | GDGCHC | 2 cut(s) | 272, 382 |
| MluCI | AATT | 7 cut(s) | 50, 216, 254, 568, 1081, 1429, 1457 |
| MlyI | GAGTC | 6 cut(s) | 401, 599, 1056, 1338, 1632, 1655 |
| MmeI | TCCRAC | 1 cut(s) | 1638 |
| Mph1103I | ATGCAT | 1 cut(s) | 1563 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1040 |
| MseI | TTAA | 4 cut(s) | 144, 648, 773, 1675 |
| MslI | CAYNNNNRTG | 5 cut(s) | 107, 210, 334, 482, 1572 |
| MspA1I | CMGCKG | 1 cut(s) | 1240 |
| MunI | CAATTG | 1 cut(s) | 568 |
| Mva1269I | GAATGC | 2 cut(s) | 472, 845 |
| MvaI | CCWGG | 5 cut(s) | 555, 1334, 1466, 1545, 1610 |
| MvnI | CGCG | 4 cut(s) | 435, 817, 1120, 1240 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 818, 839, 903, 912, 934, 996, 1113, 1266 |
| NciI | CCSGG | 6 cut(s) | 126, 282, 753, 754, 1203, 1294 |
| NdeII | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| NlaIII | CATG | 7 cut(s) | 50, 215, 745, 811, 897, 937, 1138 |
| NlaIV | GGNNCC | 5 cut(s) | 700, 751, 1100, 1297, 1500 |
| NmuCI | GTSAC | 5 cut(s) | 172, 196, 1210, 1360, 1565 |
| NsiI | ATGCAT | 1 cut(s) | 1563 |
| OliI | CACNNNNGTG | 1 cut(s) | 334 |
| PaeR7I | CTCGAG | 2 cut(s) | 574, 875 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1404 |
| PctI | GAATGC | 2 cut(s) | 472, 845 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1040 |
| PfeI | GAWTC | 2 cut(s) | 630, 1381 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1508 |
| PfoI | TCCNGGA | 1 cut(s) | 1292 |
| PinAI | ACCGGT | 1 cut(s) | 300 |
| PkrI | GCNGC | 8 cut(s) | 514, 643, 814, 844, 899, 1239, 1242, 1284 |
| PleI | GAGTC | 6 cut(s) | 401, 599, 1056, 1337, 1631, 1654 |
| PpsI | GAGTC | 6 cut(s) | 401, 599, 1056, 1337, 1631, 1654 |
| Psp6I | CCWGG | 5 cut(s) | 553, 1332, 1464, 1543, 1608 |
| PspGI | CCWGG | 5 cut(s) | 553, 1332, 1464, 1543, 1608 |
| PspN4I | GGNNCC | 5 cut(s) | 700, 751, 1100, 1297, 1500 |
| PspPI | GGNCC | 6 cut(s) | 128, 248, 279, 749, 1106, 1296 |
| PspXI | VCTCGAGB | 1 cut(s) | 574 |
| PstNI | CAGNNNCTG | 1 cut(s) | 903 |
| PsuI | RGATCY | 1 cut(s) | 1660 |
| RsaI | GTAC | 4 cut(s) | 328, 803, 881, 1500 |
| RsaNI | GTAC | 4 cut(s) | 327, 802, 880, 1499 |
| RseI | CAYNNNNRTG | 5 cut(s) | 107, 210, 334, 482, 1572 |
| SacII | CCGCGG | 1 cut(s) | 1241 |
| SaqAI | TTAA | 4 cut(s) | 144, 648, 773, 1675 |
| SatI | GCNGC | 8 cut(s) | 513, 642, 813, 843, 898, 1238, 1241, 1283 |
| Sau3AI | GATC | 4 cut(s) | 559, 1322, 1471, 1660 |
| Sau96I | GGNCC | 6 cut(s) | 128, 248, 279, 749, 1106, 1296 |
| SchI | GAGTC | 6 cut(s) | 401, 599, 1056, 1338, 1632, 1655 |
| SduI | GDGCHC | 2 cut(s) | 272, 382 |
| SexAI | ACCWGGT | 2 cut(s) | 553, 1464 |
| SfaNI | GCATC | 2 cut(s) | 111, 520 |
| Sfr274I | CTCGAG | 2 cut(s) | 574, 875 |
| Sfr303I | CCGCGG | 1 cut(s) | 1241 |
| SgrAI | CRCCGGYG | 1 cut(s) | 779 |
| SgrBI | CCGCGG | 1 cut(s) | 1241 |
| SinI | GGWCC | 2 cut(s) | 128, 1296 |
| SlaI | CTCGAG | 2 cut(s) | 574, 875 |
| SmaI | CCCGGG | 1 cut(s) | 754 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 107, 210, 334, 482, 1572 |
| SmlI | CTYRAG | 2 cut(s) | 574, 875 |
| SmoI | CTYRAG | 2 cut(s) | 574, 875 |
| SpeI | ACTAGT | 1 cut(s) | 1352 |
| Sse9I | AATT | 7 cut(s) | 50, 216, 254, 568, 1081, 1429, 1457 |
| SsiI | CCGC | 9 cut(s) | 642, 867, 975, 1128, 1227, 1235, 1238, 1240, 1496 |
| SspMI | CTAG | 3 cut(s) | 161, 1002, 1353 |
| StyI | CCWWGG | 4 cut(s) | 135, 313, 1102, 1299 |
| TaaI | ACNGT | 9 cut(s) | 109, 120, 370, 589, 670, 691, 1051, 1492, 1656 |
| TaiI | ACGT | 5 cut(s) | 198, 486, 771, 1266, 1410 |
| TaqI | TCGA | 9 cut(s) | 11, 187, 480, 575, 849, 876, 1011, 1029, 1644 |
| TasI | AATT | 7 cut(s) | 50, 216, 254, 568, 1081, 1429, 1457 |
| TatI | WGTACW | 1 cut(s) | 879 |
| TauI | GCSGC | 3 cut(s) | 644, 1240, 1243 |
| TfiI | GAWTC | 2 cut(s) | 630, 1381 |
| Tru1I | TTAA | 4 cut(s) | 144, 648, 773, 1675 |
| Tru9I | TTAA | 4 cut(s) | 144, 648, 773, 1675 |
| TscAI | CASTG | 5 cut(s) | 78, 212, 272, 594, 1509 |
| TseFI | GTSAC | 5 cut(s) | 172, 196, 1210, 1360, 1565 |
| TseI | GCWGC | 5 cut(s) | 512, 812, 842, 897, 1282 |
| Tsp45I | GTSAC | 5 cut(s) | 172, 196, 1210, 1360, 1565 |
| TspDTI | ATGAA | 4 cut(s) | 63, 380, 1151, 1373 |
| TspGWI | ACGGA | 4 cut(s) | 129, 1335, 1407, 1635 |
| TspMI | CCCGGG | 1 cut(s) | 752 |
| TspRI | CASTG | 5 cut(s) | 78, 212, 272, 594, 1509 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1508 |
| VpaK11BI | GGWCC | 2 cut(s) | 128, 1296 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 399, 1093 |
| XapI | RAATTY | 5 cut(s) | 50, 216, 254, 1081, 1457 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 230, 1509 |
| XhoI | CTCGAG | 2 cut(s) | 574, 875 |
| XmaI | CCCGGG | 1 cut(s) | 752 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1040 |
| XspI | CTAG | 3 cut(s) | 161, 1002, 1353 |
| Zsp2I | ATGCAT | 1 cut(s) | 1563 |