MD01G1158900.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001265
Physical Location & Seq
Reverse (-)
26552430 .. 26555079
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1158900.v1.1.491

Sequence Viewer

Length: 1752 bp
ATGGAGGCACTCAACGCCATTTTCAGCAACAAAATCCGACTCTCCTTATGCCTTTTCGGCCTTTGCCTTCTATTGGCTTCATCGACAATGTGTTCGGCAGAGCCCAAAACTCACCAGCATGAGTTTGTTATTCAAGCAACTAAAGTGAAGAGGCTGTGCAAAACCCACAACTCCATCACAGTAAATGGACAGTACCCTGGACCAACCTTGGAAGCAAACAATGGTGACACTCTTTTCATCAAAGTTACAAACAAAGCTCGATACAATGTCACTATTCACTGGCATGGGATTAGGCAGATTAGAACTGGATGGGCAGATGGGCCTGAATTTGTGACTCAGTGCCCGATTAGGCCAGGAGGAAGCTATACCTACCGATTTACAATTCAAGGGCAAGAAGGTACTCTCTGGTGGCATGCTCACAGCTCATGGCTTAGAGCCACCGTTTACGGCGCACTCATCATTCATCCTAAACAAGGAGACTCCTACCCCTTCACTAAACCGAAACGCGAAACAACCCTTCTTCTCGGTGAATGGTGGGACGCTAACCCTATCGATGTTATAAGGCAGGCTACTAGGACAGGAGGAGCTCCAAATGTTTCTGATGCATACACCATCAATGGTCAACCTGGTGATCTTTACAATTGCTCAAGCAAAGACACTGTCATAGTTCCTATAGACTCCGGCGAGACCAACCTTCTTAGAGTCATCAATGCTGGCCTCAACCAACCTCTTTTCTTCTCTGTGGCCAACCACAAACTCACTGTTGTTAGTGCTGATGCCTCCTATACCAAACCTTTCACTACCACGGTACTCATGCTAGGCCCTGGGCAGACCACCGATGTTTTAATCACGGGTGACCAGCCACCGGCCCGGTACTACTTGGCGGCAAGTGCTTATTTCAGCGCGCAAAATGCAGCATTCGGCAACACCACCACCACCGCCATTCTTGAATACAAGTCTGCCCCTTGCAGCCCTAGTTGCAAAAACGGTCCAACAGCTAAACCGATTATGCCACAACTCCCTGCTTTCAATGACACAAACACTGCTTCTGCTTTCACCAAGAGTTTCAGAAGTCCAAGAAAAGTTGAAGTCCCAACTGAAATCGACGAAAACCTCTTCTTCACTATCGGTCTTGGACTCAACAATTGCCCTAAACATTTCAGAGCCAGAAGGTGCCAAGGCCCAAACGGGACACGCTTCACTGCCAGCATGAACAATGTGTCCTTCGTGCTTCCAAATAACATGTCAATCCTTCAGGCTTACCAACAAAACATCCCTGGAGTTTTCACCAATGATTTTCCAGCAAACCCTCCGAAGAAATTCGACTACACGGGCAATGTGAGCAGTTCTCTATGGCAGCCTTTGTCGGGCACTAGAGGATACAACTTGAAGTATGGATCAAGGGTGCAGGTTGTGCTGCAGGACACAAGCATCGTCACACCCGAAAACCATCCTATCCATCTTCACGGATACGATTTTTACATCCTTGCCGAAGGTTTTGGAAATTTCAATGCCAAGACCGATACCAAAAAGTTCAACCTTGTTGATCCACCTATGAGGAACACAGTGGCAGTGCCTGCGAATGGATGGGCAGTTATCCGATTCGTCGCTGACAATCCAGGTGCATGGATAATGCATTGTCACTTGGATGTTCACATCAACTGGGGTTTGGCCATGGTGTTCTTGGTGGACAATGGAGTTGGGGAACTGCAAACGATCGAGCCACCACCGGCGGATCTGCCTGTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

584

Amino Acids

64.23

Weight (kDa)

8.69

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 44 - 158 2.8e-44 Multicopper oxidase
Cu-oxidase PF00394 170 - 320 2.5e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 432 - 565 1.1e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
Acc36I ACCTGC 1 cut(s) 1399
AccB1I GGYRCC 1 cut(s) 1173
AccII CGCG 2 cut(s) 507, 905
AciI CCGC 3 cut(s) 884, 939, 1733
AclWI GGATC 3 cut(s) 1405, 1541, 1743
AcoI YGGCCR 2 cut(s) 744, 1671
AcsI RAATTY 3 cut(s) 326, 1319, 1504
AcuI CTGAAG 1 cut(s) 1238
AfaI GTAC 4 cut(s) 194, 400, 810, 875
AfiI CCNNNNNNNGG 5 cut(s) 73, 473, 865, 1367, 1583
AflIII ACRYGT 1 cut(s) 1242
AgsI TTSAA 8 cut(s) 134, 386, 950, 1030, 1088, 1390, 1510, 1537
AjnI CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
AloI GAACNNNNNNTCC 2 cut(s) 1205, 1237
AluBI AGCT 5 cut(s) 257, 363, 423, 587, 998
AluI AGCT 5 cut(s) 257, 363, 423, 587, 998
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 2 cut(s) 471, 680
AlwI GGATC 3 cut(s) 1405, 1541, 1743
AlwNI CAGNNNCTG 1 cut(s) 1577
AoxI GGCC 9 cut(s) 58, 320, 350, 715, 744, 820, 867, 1180, 1671
ApeKI GCWGC 4 cut(s) 914, 969, 1357, 1417
ApoI RAATTY 3 cut(s) 326, 1319, 1504
ArsI GACNNNNNNTTYG 6 cut(s) 76, 108, 645, 677, 1229, 1261
Asp700I GAANNNNTTC 1 cut(s) 1319
AspLEI GCGC 3 cut(s) 452, 905, 907
AspS9I GGNCC 6 cut(s) 200, 320, 821, 868, 989, 1181
AsuC2I CCSGG 1 cut(s) 871
AsuHPI GGTGA 7 cut(s) 104, 236, 539, 641, 866, 1048, 1279
AvaII GGWCC 2 cut(s) 200, 989
BaeGI GKGCMC 2 cut(s) 344, 1373
BalI TGGCCA 2 cut(s) 746, 1673
BanI GGYRCC 1 cut(s) 1173
BanII GRGCYC 2 cut(s) 105, 589
Bbv12I GWGCWC 1 cut(s) 589
BbvI GCAGC 4 cut(s) 926, 981, 1369, 1404
BccI CCATC 7 cut(s) 182, 303, 311, 620, 1458, 1467, 1581
BceAI ACGGC 1 cut(s) 463
BciT130I CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
BciVI GTATCC 2 cut(s) 1373, 1463
BcnI CCSGG 1 cut(s) 871
BcoDI GTCTC 2 cut(s) 471, 680
BfaI CTAG 4 cut(s) 573, 818, 975, 1374
BfmI CTRYAG 2 cut(s) 672, 1418
BfuAI ACCTGC 1 cut(s) 1399
BfuI GTATCC 2 cut(s) 1373, 1463
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 5 cut(s) 885, 915, 970, 1358, 1418
BlsI GCNGC 5 cut(s) 886, 916, 971, 1359, 1419
Bme1390I CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
Bme18I GGWCC 2 cut(s) 200, 989
BmgT120I GGNCC 6 cut(s) 200, 320, 821, 868, 989, 1181
BmiI GGNNCC 1 cut(s) 1175
BmrFI CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
BmrI ACTGGG 1 cut(s) 1672
BmsI GCATC 3 cut(s) 592, 766, 1440
BmuI ACTGGG 1 cut(s) 1672
BplI GAGNNNNNCTC 2 cut(s) 1333, 1365
BpmI CTGGAG 1 cut(s) 1299
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 871
Bsa29I ATCGAT 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 8 cut(s) 196, 207, 804, 823, 824, 1177, 1276, 1674
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 473, 865, 1367, 1583
Bse118I RCCGGY 2 cut(s) 865, 1729
Bse1I ACTGG 3 cut(s) 284, 310, 1667
Bse3DI GCAATG 1 cut(s) 1342
BseBI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 8 cut(s) 196, 207, 804, 823, 824, 1177, 1276, 1674
BseGI GGATG 7 cut(s) 314, 463, 1272, 1450, 1482, 1592, 1654
BseLI CCNNNNNNNGG 5 cut(s) 73, 473, 865, 1367, 1583
BseMI GCAATG 1 cut(s) 1342
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 3 cut(s) 284, 310, 1667
BsePI GCGCGC 1 cut(s) 903
BseRI GAGGAG 1 cut(s) 597
BseSI GKGCMC 2 cut(s) 344, 1373
BseXI GCAGC 4 cut(s) 926, 981, 1369, 1404
BsgI GTGCAG 1 cut(s) 1427
Bsh1236I CGCG 2 cut(s) 507, 905
Bsh1285I CGRYCG 1 cut(s) 1719
BshFI GGCC 9 cut(s) 60, 322, 352, 717, 746, 822, 869, 1182, 1673
BshNI GGYRCC 1 cut(s) 1173
BshVI ATCGAT 1 cut(s) 552
BsiEI CGRYCG 1 cut(s) 1719
BsiHKAI GWGCWC 1 cut(s) 589
BsiSI CCGG 4 cut(s) 681, 866, 871, 1730
BslFI GGGAC 3 cut(s) 551, 1076, 1204
BslI CCNNNNNNNGG 5 cut(s) 73, 473, 865, 1367, 1583
BsmAI GTCTC 2 cut(s) 471, 680
BsmFI GGGAC 3 cut(s) 551, 1076, 1204
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 9 cut(s) 60, 322, 352, 717, 746, 822, 869, 1182, 1673
Bso31I GGTCTC 1 cut(s) 680
Bsp1286I GDGCHC 4 cut(s) 105, 344, 589, 1373
Bsp143I GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
Bsp19I CCATGG 1 cut(s) 1674
BspACI CCGC 3 cut(s) 884, 939, 1733
BspANI GGCC 9 cut(s) 60, 322, 352, 717, 746, 822, 869, 1182, 1673
BspCNI CTCAG 1 cut(s) 349
BspDI ATCGAT 1 cut(s) 552
BspFNI CGCG 2 cut(s) 507, 905
BspLI GGNNCC 1 cut(s) 1175
BspMAI CTGCAG 1 cut(s) 1422
BspMI ACCTGC 1 cut(s) 1399
BspPI GGATC 3 cut(s) 1405, 1541, 1743
BspT107I GGYRCC 1 cut(s) 1173
BspTNI GGTCTC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 1342
BsrFI RCCGGY 2 cut(s) 865, 1729
BsrI ACTGG 3 cut(s) 284, 310, 1667
BssAI RCCGGY 2 cut(s) 865, 1729
BssECI CCNNGG 8 cut(s) 196, 207, 804, 823, 824, 1177, 1276, 1674
BssHII GCGCGC 1 cut(s) 903
BssMI GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
BssT1I CCWWGG 3 cut(s) 207, 1177, 1674
Bst2UI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
Bst4CI ACNGT 8 cut(s) 181, 192, 442, 661, 763, 808, 989, 1567
Bst6I CTCTTC 2 cut(s) 143, 1121
BstAPI GCANNNNNTGC 2 cut(s) 1414, 1577
BstC8I GCNNGC 6 cut(s) 414, 567, 715, 905, 1207, 1578
BstDEI CTNAG 3 cut(s) 336, 431, 698
BstDSI CCRYGG 2 cut(s) 804, 1674
BstEII GGTNACC 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 7 cut(s) 314, 463, 1272, 1450, 1482, 1592, 1654
BstFNI CGCG 2 cut(s) 507, 905
BstHHI GCGC 3 cut(s) 452, 905, 907
BstKTI GATC 5 cut(s) 634, 1400, 1549, 1719, 1738
BstMAI GTCTC 2 cut(s) 471, 680
BstMBI GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
BstMCI CGRYCG 1 cut(s) 1719
BstMWI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1341, 1414, 1577
BstNI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
BstNSI RCATGY 2 cut(s) 416, 1246
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 7 cut(s) 196, 352, 625, 823, 869, 1276, 1618
BstSFI CTRYAG 2 cut(s) 672, 1418
BstSLI GKGCMC 2 cut(s) 344, 1373
BstUI CGCG 2 cut(s) 507, 905
BstV1I GCAGC 4 cut(s) 926, 981, 1369, 1404
BstX2I RGATCY 1 cut(s) 1735
BstXI CCANNNNNNTGG 1 cut(s) 1626
BstYI RGATCY 1 cut(s) 1735
Bsu15I ATCGAT 1 cut(s) 552
BsuI GTATCC 2 cut(s) 1373, 1463
BsuRI GGCC 9 cut(s) 60, 322, 352, 717, 746, 822, 869, 1182, 1673
BsuTUI ATCGAT 1 cut(s) 552
BtgI CCRYGG 2 cut(s) 804, 1674
BtsCI GGATG 7 cut(s) 314, 463, 1272, 1450, 1482, 1592, 1654
BtsI GCAGTG 3 cut(s) 1041, 1200, 1578
BtsIMutI CAGTG 8 cut(s) 277, 344, 657, 759, 1041, 1200, 1572, 1578
BveI ACCTGC 1 cut(s) 1399
Cac8I GCNNGC 6 cut(s) 414, 567, 715, 905, 1207, 1578
CaiI CAGNNNCTG 1 cut(s) 1577
CfoI GCGC 3 cut(s) 452, 905, 907
Cfr10I RCCGGY 2 cut(s) 865, 1729
Cfr13I GGNCC 6 cut(s) 200, 320, 821, 868, 989, 1181
ClaI ATCGAT 1 cut(s) 552
CseI GACGC 1 cut(s) 548
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 4 cut(s) 193, 399, 809, 874
CviAII CATG 9 cut(s) 119, 284, 413, 426, 814, 1210, 1243, 1626, 1675
CviQI GTAC 4 cut(s) 193, 399, 809, 874
DdeI CTNAG 3 cut(s) 336, 431, 698
DpnI GATC 5 cut(s) 633, 1399, 1548, 1718, 1737
DpnII GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
EaeI YGGCCR 2 cut(s) 744, 1671
Eam1104I CTCTTC 2 cut(s) 143, 1121
EarI CTCTTC 2 cut(s) 143, 1121
EciI GGCGGA 1 cut(s) 1748
Ecl136II GAGCTC 1 cut(s) 587
Eco130I CCWWGG 3 cut(s) 207, 1177, 1674
Eco24I GRGCYC 2 cut(s) 105, 589
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 200, 989
Eco53kI GAGCTC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 1238
Eco91I GGTNACC 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 821
EcoO65I GGTNACC 1 cut(s) 854
EcoRII CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
EcoT14I CCWWGG 3 cut(s) 207, 1177, 1674
EcoT22I ATGCAT 2 cut(s) 607, 1638
EcoT38I GRGCYC 2 cut(s) 105, 589
ErhI CCWWGG 3 cut(s) 207, 1177, 1674
FaeI CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1246, 1629, 1678
FaqI GGGAC 3 cut(s) 551, 1076, 1204
FatI CATG 9 cut(s) 118, 283, 412, 425, 813, 1209, 1242, 1625, 1674
Fnu4HI GCNGC 5 cut(s) 885, 915, 970, 1358, 1418
FokI GGATG 7 cut(s) 321, 450, 1259, 1437, 1469, 1599, 1661
FriOI GRGCYC 2 cut(s) 105, 589
Fsp4HI GCNGC 5 cut(s) 885, 915, 970, 1358, 1418
FspBI CTAG 4 cut(s) 573, 818, 975, 1374
GlaI GCGC 3 cut(s) 451, 904, 906
GluI GCNGC 5 cut(s) 885, 915, 970, 1358, 1418
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 9 cut(s) 60, 322, 352, 717, 746, 822, 869, 1182, 1673
HapII CCGG 4 cut(s) 681, 866, 871, 1730
HgaI GACGC 1 cut(s) 548
HhaI GCGC 3 cut(s) 452, 905, 907
Hin1II CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1246, 1629, 1678
Hin6I GCGC 3 cut(s) 450, 903, 905
HinP1I GCGC 3 cut(s) 450, 903, 905
HincII GTYRAC 1 cut(s) 623
HindII GTYRAC 1 cut(s) 623
HinfI GANTC 7 cut(s) 39, 334, 479, 677, 702, 1137, 1602
HpaII CCGG 4 cut(s) 681, 866, 871, 1730
HphI GGTGA 7 cut(s) 104, 236, 539, 641, 866, 1048, 1279
Hpy166II GTNNAC 4 cut(s) 445, 623, 1654, 1690
Hpy188I TCNGA 6 cut(s) 38, 601, 1070, 1163, 1314, 1601
Hpy188III TCNNGA 1 cut(s) 947
Hpy8I GTNNAC 4 cut(s) 445, 623, 1654, 1690
Hpy99I CGWCG 2 cut(s) 1109, 1610
HpyAV CCTTC 9 cut(s) 77, 389, 499, 527, 704, 1164, 1234, 1262, 1487
HpyCH4III ACNGT 8 cut(s) 181, 192, 442, 661, 763, 808, 989, 1567
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1341, 1414, 1577
HpyF3I CTNAG 3 cut(s) 336, 431, 698
Hsp92II CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1246, 1629, 1678
HspAI GCGC 3 cut(s) 450, 903, 905
Kzo9I GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
LmnI GCTCC 2 cut(s) 584, 592
Lsp1109I GCAGC 4 cut(s) 926, 981, 1369, 1404
LweI GCATC 3 cut(s) 592, 766, 1440
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 4 cut(s) 573, 818, 975, 1374
MaeIII GTNAC 7 cut(s) 224, 244, 268, 331, 854, 1435, 1640
MalI GATC 5 cut(s) 633, 1399, 1548, 1718, 1737
MboI GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
MboII GAAGA 7 cut(s) 160, 512, 727, 1108, 1111, 1327, 1454
MfeI CAATTG 2 cut(s) 640, 1144
MflI RGATCY 1 cut(s) 1735
MhlI GDGCHC 4 cut(s) 105, 344, 589, 1373
MlsI TGGCCA 2 cut(s) 746, 1673
MluCI AATT 6 cut(s) 326, 381, 640, 1144, 1319, 1504
MluNI TGGCCA 2 cut(s) 746, 1673
MlyI GAGTC 6 cut(s) 33, 328, 473, 671, 711, 1131
MmeI TCCRAC 2 cut(s) 61, 1016
Mox20I TGGCCA 2 cut(s) 746, 1673
Mph1103I ATGCAT 2 cut(s) 607, 1638
MroXI GAANNNNTTC 1 cut(s) 1319
MscI TGGCCA 2 cut(s) 746, 1673
MseI TTAA 2 cut(s) 845, 1750
MslI CAYNNNNRTG 3 cut(s) 117, 282, 1647
Msp20I TGGCCA 2 cut(s) 746, 1673
MspI CCGG 4 cut(s) 681, 866, 871, 1730
MspR9I CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
MunI CAATTG 2 cut(s) 640, 1144
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
MvnI CGCG 2 cut(s) 507, 905
MwoI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1341, 1414, 1577
NciI CCSGG 1 cut(s) 871
NcoI CCATGG 1 cut(s) 1674
NdeII GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
NlaIII CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1246, 1629, 1678
NlaIV GGNNCC 1 cut(s) 1175
NmuCI GTSAC 6 cut(s) 224, 268, 331, 854, 1435, 1640
NsiI ATGCAT 2 cut(s) 607, 1638
NspI RCATGY 2 cut(s) 416, 1246
PaeI GCATGC 1 cut(s) 416
PasI CCCWGGG 1 cut(s) 824
PauI GCGCGC 1 cut(s) 903
PciI ACATGT 1 cut(s) 1242
PcsI WCGNNNNNNNCGW 1 cut(s) 1440
PctI GAATGC 1 cut(s) 917
PdmI GAANNNNTTC 1 cut(s) 1319
PfeI GAWTC 1 cut(s) 1602
PflFI GACNNNGTC 1 cut(s) 659
PkrI GCNGC 5 cut(s) 886, 916, 971, 1359, 1419
Ple19I CGATCG 1 cut(s) 1719
PleI GAGTC 6 cut(s) 33, 328, 473, 671, 710, 1131
PpsI GAGTC 6 cut(s) 33, 328, 473, 671, 710, 1131
PscI ACATGT 1 cut(s) 1242
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 589
Psp6I CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
PspEI GGTNACC 1 cut(s) 854
PspGI CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
PspN4I GGNNCC 1 cut(s) 1175
PspPI GGNCC 6 cut(s) 200, 320, 821, 868, 989, 1181
PstI CTGCAG 1 cut(s) 1422
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 1 cut(s) 1735
PsyI GACNNNGTC 1 cut(s) 659
PteI GCGCGC 1 cut(s) 903
PvuI CGATCG 1 cut(s) 1719
RsaI GTAC 4 cut(s) 194, 400, 810, 875
RsaNI GTAC 4 cut(s) 193, 399, 809, 874
RseI CAYNNNNRTG 3 cut(s) 117, 282, 1647
SacI GAGCTC 1 cut(s) 589
SaqAI TTAA 2 cut(s) 845, 1750
SatI GCNGC 5 cut(s) 885, 915, 970, 1358, 1418
Sau3AI GATC 5 cut(s) 631, 1397, 1546, 1716, 1735
Sau96I GGNCC 6 cut(s) 200, 320, 821, 868, 989, 1181
SchI GAGTC 6 cut(s) 33, 328, 473, 671, 711, 1131
ScrFI CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
SduI GDGCHC 4 cut(s) 105, 344, 589, 1373
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 3 cut(s) 592, 766, 1440
SfcI CTRYAG 2 cut(s) 672, 1418
SgrAI CRCCGGYG 1 cut(s) 1729
SinI GGWCC 2 cut(s) 200, 989
SmiMI CAYNNNNRTG 3 cut(s) 117, 282, 1647
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SphI GCATGC 1 cut(s) 416
Sse9I AATT 6 cut(s) 326, 381, 640, 1144, 1319, 1504
SsiI CCGC 3 cut(s) 884, 939, 1733
SspMI CTAG 4 cut(s) 573, 818, 975, 1374
SstI GAGCTC 1 cut(s) 589
StyD4I CCNGG 7 cut(s) 196, 352, 625, 823, 869, 1276, 1618
StyI CCWWGG 3 cut(s) 207, 1177, 1674
TaaI ACNGT 8 cut(s) 181, 192, 442, 661, 763, 808, 989, 1567
TaqI TCGA 6 cut(s) 83, 259, 552, 1104, 1323, 1719
TaqII GACCGA 2 cut(s) 1118, 1535
TasI AATT 6 cut(s) 326, 381, 640, 1144, 1319, 1504
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 1 cut(s) 1602
Tru1I TTAA 2 cut(s) 845, 1750
Tru9I TTAA 2 cut(s) 845, 1750
TscAI CASTG 8 cut(s) 284, 344, 664, 766, 1048, 1207, 1572, 1578
TseFI GTSAC 6 cut(s) 224, 268, 331, 854, 1435, 1640
TseI GCWGC 4 cut(s) 914, 969, 1357, 1417
Tsp45I GTSAC 6 cut(s) 224, 268, 331, 854, 1435, 1640
TspDTI ATGAA 4 cut(s) 69, 226, 452, 1226
TspGWI ACGGA 1 cut(s) 1482
TspRI CASTG 8 cut(s) 284, 344, 664, 766, 1048, 1207, 1572, 1578
Tth111I GACNNNGTC 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 200, 989
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 3 cut(s) 326, 1319, 1504
XceI RCATGY 2 cut(s) 416, 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 1681
XmnI GAANNNNTTC 1 cut(s) 1319
XspI CTAG 4 cut(s) 573, 818, 975, 1374
Zsp2I ATGCAT 2 cut(s) 607, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.