Rh1BG134600

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001265
Physical Location & Seq
Forward (+)
22573366 .. 22575452
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG134600.1

Sequence Viewer

Length: 1677 bp
ATGTCGACCTTGGCCGATCCCAAAGCTCACCACCATGAATTTGTTATCCAAGCAACACCAGTGAAGAGGCTGTGCAAGACCCACAACACCATCACAGTGAATGGACAGTTCCCTGGACCAACCTTGGAAGTAAACAATGGTGACACTCTGGTCGTCAAAGTCACTAACAAAGCTCGATATAATGTCACCATTCACTGGCATGGCATTCGGCAAATGAGAACCGGATGGGCGGATGGGCCCGAGTTTGTTACTCAATGTCCTATTAGACCAGGAGGGAGTTACACCTACCGGTTCACAGTCCAAGGTCAAGAAGGTACTCTGTGGTGGCACGCTCATAGCTCATGGCTTAGAGCCACCGTGTATGGAGCACTTATTATTCATCCGAAACAAGGAGACTCGTATCCATTCACTAAACCGAATCGTCAAGCAACACTTCTTCTTGGTGAGTGGTGGGATGGTAACCCTATTGACGTGGTGAGGAGGGCGACTCGCACAGGAGCAGCTCCAAATGTTTCTAACGCATATACCATTAACGGTCAACCCGGAGATCTTTACAGTTGCTCCAGCAAAGGCACCGTCATAGTTCCAATAGACTCTGGTGAGACCAACCTACTGAGAGTGATAAACGCCGCCCTCAACCAACCTCTTTTCTTCTCCGTCGCCAACCATAAACTGACTGTGGTTGGTGCTGATGCCTCGTACACCAAGCCTTTCACCACAAAAGTTCTCATGTTAGGCCCAGGCCAGACTACTGATGTTTTAATCACCGGGGACCAGCCACCATCTCGATACTACTTGGCGGCGAGTGCCTATTTCAGTGCACAAAATGCTGCATTTGATAACACCACCACCACAGCCATTCTTGAATACAAGTCCGTTCGTTGCAACACTACAAGTTGCAAAAAGGGTAAAACAGTAATTAAACCCACAATGCCAAAGCTGCCAGCTTTCAATGACACAAACACTGCATCTGCCTTCACTAAGAGCTTCAGAAGCCCTAGAAAAGTCGAAGTCCCAACTGAAATCGACGAGAACCTCTTCTTCACAATCGGCCTCGGACTCAACAAATGCCCTAAGCACTTTCGATCTAGAAGGTGCCAAGGCCCCAATGGCACACGCTTCACTGCTAGCATGAACAATGTCTCATTTGTGCTTCCAAACAACATGTCAATTCTCCAAGCTTACCAGCAAAACATTCCTGGAGTTTACACTAGTGATTTTCCAGCCAACCCGCCACGGAAGTTTGATTATACTGGGAATGTCAGCCGCTCGCTTTGGCAACCCAGTTCTGGAACAAAGGGATACAAGTTGAAGTACGGGTCTAGGGTGCAGGTGGTGTTACAAGACACAAGTATTGTCACACCCGAGAACCATCCCATTCATCTCCACGGTTATGATTTCTACATCCTTGCAGAGGGGTTTGGAAATTTCAACCCCAAGACTGATACTAAAATGTTTAATCTTGTTGATCCACCTCTAAGGAACACGGTCGCGGTGCCCGTAAATGGTTGGGCAGTCATCAGATTTGTAGCTGACAATCCAGGTGCTTGGATGATGCATTGTCACTTGGATGTTCATATCAACTGGGGTTTGGCCATGGTTTTCTTGGTGGACAATGGAGTTGGGGAGCTGCAATCAATAGAGCCACCGCCAGTGGATTATCCTCTGTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

558

Amino Acids

61.78

Weight (kDa)

9.26

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 16 - 130 1.7e-45 Multicopper oxidase
Cu-oxidase PF00394 143 - 291 1.1e-40 Multicopper oxidase
Cu-oxidase_2 PF07731 406 - 539 3.5e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1321
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 1321
AccB1I GGYRCC 3 cut(s) 572, 1095, 1495
AccB7I CCANNNNNTGG 1 cut(s) 195
AccBSI CCGCTC 1 cut(s) 1269
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1493
AciI CCGC 7 cut(s) 230, 630, 800, 1232, 1267, 1493, 1649
AclWI GGATC 2 cut(s) 11, 1463
AcoI YGGCCR 2 cut(s) 12, 1593
AcsI RAATTY 2 cut(s) 38, 1426
AcuI CTGAAG 1 cut(s) 973
AfaI GTAC 3 cut(s) 316, 701, 1316
AfiI CCNNNNNNNGG 5 cut(s) 195, 389, 1289, 1414, 1505
AflIII ACRYGT 1 cut(s) 1164
AgeI ACCGGT 1 cut(s) 288
AgsI TTSAA 4 cut(s) 866, 952, 1312, 1432
AhlI ACTAGT 1 cut(s) 1211
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 5 cut(s) 112, 268, 739, 1198, 1540
Alw21I GWGCWC 2 cut(s) 370, 823
Alw26I GTCTC 3 cut(s) 387, 596, 1147
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 2 cut(s) 11, 1463
Ama87I CYCGRG 2 cut(s) 239, 1364
AoxI GGCC 7 cut(s) 12, 236, 736, 742, 1051, 1102, 1593
ApaI GGGCCC 1 cut(s) 240
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 4 cut(s) 500, 830, 940, 1630
ApoI RAATTY 2 cut(s) 38, 1426
ArsI GACNNNNNNTTYG 4 cut(s) 561, 593, 1151, 1183
AsiGI ACCGGT 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 1037
AspS9I GGNCC 6 cut(s) 116, 236, 237, 737, 772, 1103
AsuC2I CCSGG 2 cut(s) 543, 769
AsuHPI GGTGA 8 cut(s) 20, 152, 178, 455, 487, 611, 706, 757
AsuNHI GCTAGC 1 cut(s) 1127
AvaI CYCGRG 2 cut(s) 239, 1364
AvaII GGWCC 2 cut(s) 116, 772
BaeGI GKGCMC 3 cut(s) 240, 823, 1500
BalI TGGCCA 1 cut(s) 1595
BanI GGYRCC 3 cut(s) 572, 1095, 1495
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 2 cut(s) 370, 823
BbvI GCAGC 4 cut(s) 512, 817, 927, 1617
BccI CCATC 6 cut(s) 98, 219, 227, 449, 790, 1380
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 5 cut(s) 114, 270, 741, 1200, 1542
BciVI GTATCC 2 cut(s) 411, 1295
BcnI CCSGG 2 cut(s) 543, 769
BcoDI GTCTC 3 cut(s) 387, 596, 1147
BcuI ACTAGT 1 cut(s) 1211
BfaI CTAG 5 cut(s) 999, 1089, 1128, 1212, 1323
BfuAI ACCTGC 1 cut(s) 1321
BfuI GTATCC 2 cut(s) 411, 1295
BglI GCCNNNNNGGC 1 cut(s) 1110
BglII AGATCT 1 cut(s) 547
BisI GCNGC 7 cut(s) 501, 630, 801, 831, 941, 1267, 1631
BlsI GCNGC 7 cut(s) 502, 631, 802, 832, 942, 1268, 1632
Bme1390I CCNGG 7 cut(s) 114, 270, 543, 741, 769, 1200, 1542
Bme18I GGWCC 2 cut(s) 116, 772
BmeT110I CYCGRG 2 cut(s) 239, 1364
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 6 cut(s) 116, 236, 237, 737, 772, 1103
BmiI GGNNCC 6 cut(s) 238, 574, 773, 1097, 1105, 1497
BmrFI CCNGG 7 cut(s) 114, 270, 543, 741, 769, 1200, 1542
BmrI ACTGGG 3 cut(s) 1263, 1278, 1594
BmsI GCATC 3 cut(s) 682, 977, 1545
BmtI GCTAGC 1 cut(s) 1131
BmuI ACTGGG 3 cut(s) 1263, 1278, 1594
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpmI CTGGAG 2 cut(s) 547, 1221
Bpu10I CCTNAGC 1 cut(s) 1074
BpuMI CCSGG 2 cut(s) 543, 769
BsaI GGTCTC 1 cut(s) 596
BsaWI WCCGGW 2 cut(s) 221, 288
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 389, 1289, 1414, 1505
Bse118I RCCGGY 1 cut(s) 288
Bse1I ACTGG 6 cut(s) 59, 200, 1258, 1284, 1589, 1652
BseBI CCWGG 5 cut(s) 114, 270, 741, 1200, 1542
BseGI GGATG 8 cut(s) 230, 238, 379, 460, 1372, 1404, 1557, 1576
BseLI CCNNNNNNNGG 5 cut(s) 195, 389, 1289, 1414, 1505
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 6 cut(s) 59, 200, 1258, 1284, 1589, 1652
BseRI GAGGAG 1 cut(s) 493
BseSI GKGCMC 3 cut(s) 240, 823, 1500
BseXI GCAGC 4 cut(s) 512, 817, 927, 1617
BsgI GTGCAG 1 cut(s) 1349
Bsh1236I CGCG 1 cut(s) 1493
Bsh1285I CGRYCG 1 cut(s) 1491
BshFI GGCC 7 cut(s) 14, 238, 738, 744, 1053, 1104, 1595
BshNI GGYRCC 3 cut(s) 572, 1095, 1495
BshTI ACCGGT 1 cut(s) 288
BsiEI CGRYCG 1 cut(s) 1491
BsiHKAI GWGCWC 2 cut(s) 370, 823
BsiHKCI CYCGRG 2 cut(s) 239, 1364
BsiSI CCGG 4 cut(s) 222, 289, 543, 768
BslFI GGGAC 2 cut(s) 785, 998
BslI CCNNNNNNNGG 5 cut(s) 195, 389, 1289, 1414, 1505
BsmAI GTCTC 3 cut(s) 387, 596, 1147
BsmFI GGGAC 2 cut(s) 785, 998
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 7 cut(s) 14, 238, 738, 744, 1053, 1104, 1595
Bso31I GGTCTC 1 cut(s) 596
BsoBI CYCGRG 2 cut(s) 239, 1364
Bsp120I GGGCCC 1 cut(s) 236
Bsp1286I GDGCHC 4 cut(s) 240, 370, 823, 1500
Bsp143I GATC 4 cut(s) 16, 547, 1085, 1468
Bsp19I CCATGG 1 cut(s) 1596
BspACI CCGC 7 cut(s) 230, 630, 800, 1232, 1267, 1493, 1649
BspANI GGCC 7 cut(s) 14, 238, 738, 744, 1053, 1104, 1595
BspCNI CTCAG 1 cut(s) 606
BspFNI CGCG 1 cut(s) 1493
BspLI GGNNCC 6 cut(s) 238, 574, 773, 1097, 1105, 1497
BspMI ACCTGC 1 cut(s) 1321
BspOI GCTAGC 1 cut(s) 1131
BspPI GGATC 2 cut(s) 11, 1463
BspT107I GGYRCC 3 cut(s) 572, 1095, 1495
BspTNI GGTCTC 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 1269
BsrFI RCCGGY 1 cut(s) 288
BsrI ACTGG 6 cut(s) 59, 200, 1258, 1284, 1589, 1652
BssAI RCCGGY 1 cut(s) 288
BssMI GATC 4 cut(s) 16, 547, 1085, 1468
BssT1I CCWWGG 5 cut(s) 9, 123, 301, 1099, 1596
Bst2UI CCWGG 5 cut(s) 114, 270, 741, 1200, 1542
Bst6I CTCTTC 2 cut(s) 59, 1043
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 4 cut(s) 330, 945, 1129, 1271
BstDEI CTNAG 5 cut(s) 347, 614, 981, 1074, 1478
BstDSI CCRYGG 3 cut(s) 1235, 1387, 1596
BstEII GGTNACC 1 cut(s) 458
BstENI CCTNNNNNAGG 1 cut(s) 1412
BstF5I GGATG 8 cut(s) 230, 238, 379, 460, 1372, 1404, 1557, 1576
BstFNI CGCG 1 cut(s) 1493
BstKTI GATC 4 cut(s) 19, 550, 1088, 1471
BstMAI GTCTC 3 cut(s) 387, 596, 1147
BstMBI GATC 4 cut(s) 16, 547, 1085, 1468
BstMCI CGRYCG 1 cut(s) 1491
BstMWI GCNNNNNNNGC 5 cut(s) 806, 827, 940, 993, 1110
BstNI CCWGG 5 cut(s) 114, 270, 741, 1200, 1542
BstNSI RCATGY 1 cut(s) 1168
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 7 cut(s) 112, 268, 541, 739, 767, 1198, 1540
BstSLI GKGCMC 3 cut(s) 240, 823, 1500
BstUI CGCG 1 cut(s) 1493
BstV1I GCAGC 4 cut(s) 512, 817, 927, 1617
BstX2I RGATCY 1 cut(s) 547
BstXI CCANNNNNNTGG 1 cut(s) 1548
BstYI RGATCY 1 cut(s) 547
BsuI GTATCC 2 cut(s) 411, 1295
BsuRI GGCC 7 cut(s) 14, 238, 738, 744, 1053, 1104, 1595
BtgI CCRYGG 3 cut(s) 1235, 1387, 1596
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 8 cut(s) 230, 238, 379, 460, 1372, 1404, 1557, 1576
BtsI GCAGTG 2 cut(s) 963, 1122
BtsIMutI CAGTG 7 cut(s) 66, 102, 193, 823, 963, 1122, 1659
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 4 cut(s) 330, 945, 1129, 1271
Cfr10I RCCGGY 1 cut(s) 288
Cfr13I GGNCC 6 cut(s) 116, 236, 237, 737, 772, 1103
Csp6I GTAC 3 cut(s) 315, 700, 1315
CspAI ACCGGT 1 cut(s) 288
CviAII CATG 7 cut(s) 35, 200, 342, 730, 1132, 1165, 1597
CviQI GTAC 3 cut(s) 315, 700, 1315
DdeI CTNAG 5 cut(s) 347, 614, 981, 1074, 1478
DpnI GATC 4 cut(s) 18, 549, 1087, 1470
DpnII GATC 4 cut(s) 16, 547, 1085, 1468
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 12, 1593
Eam1104I CTCTTC 2 cut(s) 59, 1043
EarI CTCTTC 2 cut(s) 59, 1043
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 5 cut(s) 9, 123, 301, 1099, 1596
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 596
Eco47I GGWCC 2 cut(s) 116, 772
Eco57I CTGAAG 1 cut(s) 973
Eco88I CYCGRG 2 cut(s) 239, 1364
Eco91I GGTNACC 1 cut(s) 458
EcoNI CCTNNNNNAGG 1 cut(s) 1412
EcoO109I RGGNCCY 1 cut(s) 1103
EcoO65I GGTNACC 1 cut(s) 458
EcoRII CCWGG 5 cut(s) 112, 268, 739, 1198, 1540
EcoT14I CCWWGG 5 cut(s) 9, 123, 301, 1099, 1596
EcoT22I ATGCAT 1 cut(s) 1560
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 5 cut(s) 9, 123, 301, 1099, 1596
FaeI CATG 7 cut(s) 38, 203, 345, 733, 1135, 1168, 1600
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 2 cut(s) 785, 998
FatI CATG 7 cut(s) 34, 199, 341, 729, 1131, 1164, 1596
FauI CCCGC 1 cut(s) 1239
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 7 cut(s) 501, 630, 801, 831, 941, 1267, 1631
FokI GGATG 8 cut(s) 237, 245, 366, 467, 1359, 1391, 1564, 1583
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 7 cut(s) 501, 630, 801, 831, 941, 1267, 1631
FspBI CTAG 5 cut(s) 999, 1089, 1128, 1212, 1323
GluI GCNGC 7 cut(s) 501, 630, 801, 831, 941, 1267, 1631
GsuI CTGGAG 2 cut(s) 547, 1221
HaeIII GGCC 7 cut(s) 14, 238, 738, 744, 1053, 1104, 1595
HapII CCGG 4 cut(s) 222, 289, 543, 768
Hin1II CATG 7 cut(s) 38, 203, 345, 733, 1135, 1168, 1600
HincII GTYRAC 2 cut(s) 6, 539
HindII GTYRAC 2 cut(s) 6, 539
HindIII AAGCTT 1 cut(s) 1179
HinfI GANTC 5 cut(s) 395, 418, 487, 593, 1059
HpaII CCGG 4 cut(s) 222, 289, 543, 768
HphI GGTGA 8 cut(s) 20, 152, 178, 455, 487, 611, 706, 757
Hpy166II GTNNAC 8 cut(s) 6, 133, 294, 539, 702, 821, 1207, 1612
Hpy188I TCNGA 4 cut(s) 384, 992, 1058, 1523
Hpy188III TCNNGA 5 cut(s) 308, 786, 863, 1089, 1290
Hpy8I GTNNAC 8 cut(s) 6, 133, 294, 539, 702, 821, 1207, 1612
Hpy99I CGWCG 2 cut(s) 662, 1031
HpyAV CCTTC 3 cut(s) 305, 985, 1086
HpyCH4IV ACGT 1 cut(s) 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 806, 827, 940, 993, 1110
HpyF3I CTNAG 5 cut(s) 347, 614, 981, 1074, 1478
HpySE526I ACGT 1 cut(s) 471
Hsp92II CATG 7 cut(s) 38, 203, 345, 733, 1135, 1168, 1600
Kzo9I GATC 4 cut(s) 16, 547, 1085, 1468
LmnI GCTCC 5 cut(s) 365, 497, 508, 566, 1627
Lsp1109I GCAGC 4 cut(s) 512, 817, 927, 1617
LweI GCATC 3 cut(s) 682, 977, 1545
MaeI CTAG 5 cut(s) 999, 1089, 1128, 1212, 1323
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 9 cut(s) 140, 160, 184, 247, 278, 458, 1338, 1357, 1562
MalI GATC 4 cut(s) 18, 549, 1087, 1470
MbiI CCGCTC 1 cut(s) 1269
MboI GATC 4 cut(s) 16, 547, 1085, 1468
MboII GAAGA 5 cut(s) 76, 428, 643, 1030, 1033
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 4 cut(s) 240, 370, 823, 1500
MlsI TGGCCA 1 cut(s) 1595
MluCI AATT 4 cut(s) 38, 918, 1170, 1426
MluNI TGGCCA 1 cut(s) 1595
MlyI GAGTC 4 cut(s) 389, 481, 587, 1053
Mox20I TGGCCA 1 cut(s) 1595
Mph1103I ATGCAT 1 cut(s) 1560
MroXI GAANNNNTTC 1 cut(s) 1037
MscI TGGCCA 1 cut(s) 1595
MseI TTAA 4 cut(s) 531, 761, 921, 1458
MslI CAYNNNNRTG 5 cut(s) 33, 95, 198, 1392, 1569
Msp20I TGGCCA 1 cut(s) 1595
MspI CCGG 4 cut(s) 222, 289, 543, 768
MspR9I CCNGG 7 cut(s) 114, 270, 543, 741, 769, 1200, 1542
Mva1269I GAATGC 1 cut(s) 204
MvaI CCWGG 5 cut(s) 114, 270, 741, 1200, 1542
MvnI CGCG 1 cut(s) 1493
MwoI GCNNNNNNNGC 5 cut(s) 806, 827, 940, 993, 1110
NciI CCSGG 2 cut(s) 543, 769
NcoI CCATGG 1 cut(s) 1596
NdeII GATC 4 cut(s) 16, 547, 1085, 1468
NheI GCTAGC 1 cut(s) 1127
NlaIII CATG 7 cut(s) 38, 203, 345, 733, 1135, 1168, 1600
NlaIV GGNNCC 6 cut(s) 238, 574, 773, 1097, 1105, 1497
NmuCI GTSAC 5 cut(s) 140, 160, 184, 1357, 1562
NsiI ATGCAT 1 cut(s) 1560
NspI RCATGY 1 cut(s) 1168
PaqCI CACCTGC 1 cut(s) 1321
PciI ACATGT 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 1497
PctI GAATGC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 1 cut(s) 418
PflMI CCANNNNNTGG 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 1198
PinAI ACCGGT 1 cut(s) 288
PkrI GCNGC 7 cut(s) 502, 631, 802, 832, 942, 1268, 1632
PleI GAGTC 4 cut(s) 389, 481, 587, 1053
PpsI GAGTC 4 cut(s) 389, 481, 587, 1053
PscI ACATGT 1 cut(s) 1164
Psp6I CCWGG 5 cut(s) 112, 268, 739, 1198, 1540
PspEI GGTNACC 1 cut(s) 458
PspGI CCWGG 5 cut(s) 112, 268, 739, 1198, 1540
PspN4I GGNNCC 6 cut(s) 238, 574, 773, 1097, 1105, 1497
PspOMI GGGCCC 1 cut(s) 236
PspPI GGNCC 6 cut(s) 116, 236, 237, 737, 772, 1103
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 3 cut(s) 316, 701, 1316
RsaNI GTAC 3 cut(s) 315, 700, 1315
RseI CAYNNNNRTG 5 cut(s) 33, 95, 198, 1392, 1569
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 531, 761, 921, 1458
SatI GCNGC 7 cut(s) 501, 630, 801, 831, 941, 1267, 1631
Sau3AI GATC 4 cut(s) 16, 547, 1085, 1468
Sau96I GGNCC 6 cut(s) 116, 236, 237, 737, 772, 1103
SchI GAGTC 4 cut(s) 389, 481, 587, 1053
ScrFI CCNGG 7 cut(s) 114, 270, 543, 741, 769, 1200, 1542
SduI GDGCHC 4 cut(s) 240, 370, 823, 1500
SfaNI GCATC 3 cut(s) 682, 977, 1545
SinI GGWCC 2 cut(s) 116, 772
SmiMI CAYNNNNRTG 5 cut(s) 33, 95, 198, 1392, 1569
SpeI ACTAGT 1 cut(s) 1211
Sse9I AATT 4 cut(s) 38, 918, 1170, 1426
SsiI CCGC 7 cut(s) 230, 630, 800, 1232, 1267, 1493, 1649
SspMI CTAG 5 cut(s) 999, 1089, 1128, 1212, 1323
StyD4I CCNGG 7 cut(s) 112, 268, 541, 739, 767, 1198, 1540
StyI CCWWGG 5 cut(s) 9, 123, 301, 1099, 1596
TaiI ACGT 1 cut(s) 474
TaqI TCGA 6 cut(s) 5, 175, 787, 1008, 1026, 1084
TasI AATT 4 cut(s) 38, 918, 1170, 1426
TauI GCSGC 3 cut(s) 632, 803, 1269
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 4 cut(s) 531, 761, 921, 1458
Tru9I TTAA 4 cut(s) 531, 761, 921, 1458
TscAI CASTG 7 cut(s) 66, 102, 200, 823, 970, 1129, 1659
TseFI GTSAC 5 cut(s) 140, 160, 184, 1357, 1562
TseI GCWGC 4 cut(s) 500, 830, 940, 1630
Tsp45I GTSAC 5 cut(s) 140, 160, 184, 1357, 1562
TspDTI ATGAA 5 cut(s) 51, 368, 1148, 1370, 1565
TspGWI ACGGA 3 cut(s) 646, 865, 1252
TspRI CASTG 7 cut(s) 66, 102, 200, 823, 970, 1129, 1659
Van91I CCANNNNNTGG 1 cut(s) 195
VneI GTGCAC 1 cut(s) 819
VpaK11BI GGWCC 2 cut(s) 116, 772
XagI CCTNNNNNAGG 1 cut(s) 1412
XapI RAATTY 2 cut(s) 38, 1426
XbaI TCTAGA 1 cut(s) 1088
XceI RCATGY 1 cut(s) 1168
XcmI CCANNNNNNNNNTGG 2 cut(s) 1106, 1603
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 5 cut(s) 999, 1089, 1128, 1212, 1323
Zsp2I ATGCAT 1 cut(s) 1560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.