pycom01g17570

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001265
Physical Location & Seq
Reverse (-)
16816615 .. 16818925
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17570.2

Sequence Viewer

Length: 1752 bp
ATGGAGGCACTCAACGCTATTTTCGTCAACAAGATTCGCTTCACGTTATGCCTTTTCGGCCTTTGCCTTCTCGTGGCTTCATCGACAATGTCCTTGGCAGGACCCCAAATTCACAAGCATGAGTTTGTTGTTCAAGCAACACCAGTGAACAGGCTGTGCAAAACCCAAAACTCCATCACAGTGAATGGACAGCTCCCTGGACCAACCTTGGAAGTAAACAATGGTGACACTCTTATCGTCAAAGTCACCAACAGAGCTCAATACAACGTCACCATCCACTGGCATGGGATTAGGCAAATGAGAACTGGATGGGCAGATGGGCCAGAATTTGTGACTCAGTGCCCAATTAGGCCAGGAGGGAGCTATACCTACCGCTTTACAATTCAAGGGCAAGAAGGTACCCTGTGGTGGCATGCTCACAGCTCATGGCTTAGAGCAACCGTTTACGGAGCACTCATCATTCATCCTAAACTAGGAGACTCCTACCCCTTCACTAAACCAAAACGCGAAACAACCCTTCTTCTCGGTGAATGGTGGAACGCTAACCCTATCGATGTCTTGAGGCAGGCGACTATGACAGGAGGAGCTCCAAATGTTTCTGATGCATACACCATCAATGGTCAACCTGGTGATCTTTACAACTGCTCAAGCCAAGACACCGTCATAGTTCCTATAGACTCCGGCGAGACCAACCTTCTTAGAGTCATCAACGCTGCACTCAACCAACCTCTTTTCTTCTCCGTGGCCAACCACAAGCTTACCGTTGTTAGTGCTGATGCCTCCTACACCAAACCTTTCACTACCACAGTTCTCATGTTAGGGCCTGGGCAGACCACCGATGTTTTAATCACCGGTGACCAGTCACCAGCCCGGTACTACTTGGCGGCGAGTGCTTATTTCAGTGCGCAAAATGCAGCATTCGACAACACCACCACCACCGCCATTCTTGAATACAAGTCTGCCCCTTGCAGCCCCAATTGCAAAAACAGTTCAGCAGCCAAACCAATTATGCCACAACTCCCTGCTTTCAATGACACAAACACTGCTTCCGCTTTCACCAAGAGTTTCAGAAGTCCAAGAAAAGTTGAAGTCCCAACTGAAATCGATGAAAATCTCTTCTTCACAATTGGTCTTGGACTCAACAACTGCCCGAAAAATTTTGGATCCCAAAGGTGCCAAGCCCCGAACGGGACACGCTTCACAGCCAGCATGAATAATGTGTCCTTCGTGCTTCCAAACAACATTTCAATCCTTCAGGCATACCAACAAAACATCCCTGGAGTTTTCACTGCTGATTTTCCAGCAAACCCTCCGACGAAATTCGACTACACTGGCAATGTGAGCCGCTCTCTCTGGCAACCAATGTCAGGCACTAGAGGATACAAGTTGAAGTATGGATCGATCATGCAGGTTGTGCTGCAGGACACAAGCATCGTCACACCAGAAAACCATCCTATCCATCTTCACGGATACGATTTTTACATCATTGCTGAGGGTTTTGGAAATTTCAATGCCCAGACTGATACCAAAAAGTTCAACCTTGTTGATCCACCTATGAGGAACACAGTGGCAGTGCCTGCGAATGGATGGGCAGTCATTCGATTTGTCGCTGACAATCCAGGTGCATGGATAATGCATTGTCACTTGGATGTCCACATCAATTGGGGTTTGGCCATGGTGTTCTTGGTGGACAATGGAGTTGGGGAACTGCAGTCAGTCGAGCAACCACCGGCGGATCTGCCTCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

584

Amino Acids

64.05

Weight (kDa)

7.2

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 906
Acc36I ACCTGC 1 cut(s) 1399
Acc65I GGTACC 1 cut(s) 398
AccB1I GGYRCC 2 cut(s) 398, 1173
AccB7I CCANNNNNTGG 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 1347
AccII CGCG 1 cut(s) 507
AciI CCGC 6 cut(s) 373, 884, 939, 1050, 1345, 1733
AclWI GGATC 5 cut(s) 1158, 1171, 1405, 1541, 1743
AcoI YGGCCR 2 cut(s) 744, 1671
AcsI RAATTY 5 cut(s) 108, 326, 1156, 1319, 1504
AcuI CTGAAG 1 cut(s) 1238
AfaI GTAC 2 cut(s) 400, 875
AfiI CCNNNNNNNGG 7 cut(s) 73, 279, 408, 473, 1188, 1367, 1583
AgeI ACCGGT 1 cut(s) 851
AgsI TTSAA 9 cut(s) 134, 386, 950, 1030, 1088, 1248, 1390, 1510, 1537
AjnI CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
AjuI GAANNNNNNNTTGG 2 cut(s) 1228, 1260
AluBI AGCT 6 cut(s) 193, 257, 363, 423, 587, 757
AluI AGCT 6 cut(s) 193, 257, 363, 423, 587, 757
Alw21I GWGCWC 3 cut(s) 259, 454, 589
Alw26I GTCTC 2 cut(s) 471, 680
AlwI GGATC 5 cut(s) 1158, 1171, 1405, 1541, 1743
AlwNI CAGNNNCTG 1 cut(s) 1577
AoxI GGCC 6 cut(s) 58, 320, 350, 744, 821, 1671
ApeKI GCWGC 5 cut(s) 713, 914, 969, 995, 1417
ApoI RAATTY 5 cut(s) 108, 326, 1156, 1319, 1504
AsiGI ACCGGT 1 cut(s) 851
Asp718I GGTACC 1 cut(s) 398
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 4 cut(s) 101, 200, 320, 821
AsuC2I CCSGG 1 cut(s) 871
AsuHPI GGTGA 9 cut(s) 236, 238, 262, 539, 641, 841, 855, 866, 1048
AvaII GGWCC 2 cut(s) 101, 200
BaeGI GKGCMC 1 cut(s) 344
BalI TGGCCA 2 cut(s) 746, 1673
BamHI GGATCC 1 cut(s) 1163
BanI GGYRCC 2 cut(s) 398, 1173
BanII GRGCYC 2 cut(s) 259, 589
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 3 cut(s) 259, 454, 589
BbvCI CCTCAGC 1 cut(s) 1491
BbvI GCAGC 5 cut(s) 700, 926, 981, 1007, 1404
BccI CCATC 8 cut(s) 182, 281, 303, 311, 620, 1458, 1467, 1581
BciT130I CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
BciVI GTATCC 2 cut(s) 1373, 1463
BcnI CCSGG 1 cut(s) 871
BcoDI GTCTC 2 cut(s) 471, 680
BfaI CTAG 2 cut(s) 473, 1374
BfmI CTRYAG 3 cut(s) 672, 1418, 1709
BfuAI ACCTGC 1 cut(s) 1399
BfuI GTATCC 2 cut(s) 1373, 1463
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 7 cut(s) 714, 885, 915, 970, 996, 1345, 1418
BlsI GCNGC 7 cut(s) 715, 886, 916, 971, 997, 1346, 1419
Bme1390I CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
Bme18I GGWCC 2 cut(s) 101, 200
BmgT120I GGNCC 4 cut(s) 101, 200, 320, 821
BmiI GGNNCC 4 cut(s) 103, 400, 1165, 1175
BmrFI CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
BmsI GCATC 3 cut(s) 592, 766, 1440
BplI GAGNNNNNCTC 2 cut(s) 1333, 1365
BpmI CTGGAG 1 cut(s) 1299
Bpu10I CCTNAGC 1 cut(s) 1491
BpuEI CTTGAG 2 cut(s) 580, 631
BpuMI CCSGG 1 cut(s) 871
Bsa29I ATCGAT 3 cut(s) 552, 1104, 1400
BsaBI GATNNNNATC 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 7 cut(s) 93, 196, 207, 741, 824, 1276, 1674
BsaWI WCCGGW 1 cut(s) 851
Bsc4I CCNNNNNNNGG 7 cut(s) 73, 279, 408, 473, 1188, 1367, 1583
Bse118I RCCGGY 2 cut(s) 851, 1729
Bse1I ACTGG 5 cut(s) 143, 284, 310, 859, 1336
Bse3DI GCAATG 2 cut(s) 1342, 1485
Bse8I GATNNNNATC 1 cut(s) 1110
BseBI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
BseCI ATCGAT 3 cut(s) 552, 1104, 1400
BseDI CCNNGG 7 cut(s) 93, 196, 207, 741, 824, 1276, 1674
BseGI GGATG 7 cut(s) 273, 314, 463, 1272, 1450, 1592, 1654
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 7 cut(s) 73, 279, 408, 473, 1188, 1367, 1583
BseMI GCAATG 2 cut(s) 1342, 1485
BseMII CTCAG 2 cut(s) 350, 1482
BseNI ACTGG 5 cut(s) 143, 284, 310, 859, 1336
BseRI GAGGAG 1 cut(s) 597
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 5 cut(s) 700, 926, 981, 1007, 1404
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 1 cut(s) 507
BshFI GGCC 6 cut(s) 60, 322, 352, 746, 823, 1673
BshNI GGYRCC 2 cut(s) 398, 1173
BshTI ACCGGT 1 cut(s) 851
BshVI ATCGAT 3 cut(s) 552, 1104, 1400
BsiHKAI GWGCWC 3 cut(s) 259, 454, 589
BsiSI CCGG 4 cut(s) 681, 852, 871, 1730
BslFI GGGAC 2 cut(s) 1076, 1204
BslI CCNNNNNNNGG 7 cut(s) 73, 279, 408, 473, 1188, 1367, 1583
BsmAI GTCTC 2 cut(s) 471, 680
BsmFI GGGAC 2 cut(s) 1076, 1204
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 6 cut(s) 60, 322, 352, 746, 823, 1673
Bso31I GGTCTC 1 cut(s) 680
Bsp1286I GDGCHC 4 cut(s) 259, 344, 454, 589
Bsp143I GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
Bsp19I CCATGG 1 cut(s) 1674
BspACI CCGC 6 cut(s) 373, 884, 939, 1050, 1345, 1733
BspANI GGCC 6 cut(s) 60, 322, 352, 746, 823, 1673
BspCNI CTCAG 2 cut(s) 349, 1483
BspDI ATCGAT 3 cut(s) 552, 1104, 1400
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 4 cut(s) 103, 400, 1165, 1175
BspMAI CTGCAG 2 cut(s) 1422, 1713
BspMI ACCTGC 1 cut(s) 1399
BspPI GGATC 5 cut(s) 1158, 1171, 1405, 1541, 1743
BspT107I GGYRCC 2 cut(s) 398, 1173
BspTNI GGTCTC 1 cut(s) 680
BsrBI CCGCTC 1 cut(s) 1347
BsrDI GCAATG 2 cut(s) 1342, 1485
BsrFI RCCGGY 2 cut(s) 851, 1729
BsrI ACTGG 5 cut(s) 143, 284, 310, 859, 1336
BssAI RCCGGY 2 cut(s) 851, 1729
BssECI CCNNGG 7 cut(s) 93, 196, 207, 741, 824, 1276, 1674
BssMI GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 3 cut(s) 93, 207, 1674
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
Bst4CI ACNGT 7 cut(s) 181, 442, 661, 763, 808, 989, 1567
Bst6I CTCTTC 1 cut(s) 1121
BstAPI GCANNNNNTGC 2 cut(s) 1414, 1577
BstC8I GCNNGC 4 cut(s) 414, 567, 1207, 1578
BstDEI CTNAG 4 cut(s) 336, 431, 698, 1491
BstDSI CCRYGG 2 cut(s) 741, 1674
BstEII GGTNACC 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 7 cut(s) 273, 314, 463, 1272, 1450, 1592, 1654
BstFNI CGCG 1 cut(s) 507
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 6 cut(s) 634, 1166, 1400, 1404, 1549, 1738
BstMAI GTCTC 2 cut(s) 471, 680
BstMBI GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
BstMWI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1341, 1414, 1577
BstNI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
BstNSI RCATGY 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 7 cut(s) 196, 352, 625, 823, 869, 1276, 1618
BstSFI CTRYAG 3 cut(s) 672, 1418, 1709
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 5 cut(s) 700, 926, 981, 1007, 1404
BstX2I RGATCY 2 cut(s) 1163, 1735
BstXI CCANNNNNNTGG 2 cut(s) 284, 1626
BstYI RGATCY 2 cut(s) 1163, 1735
Bsu15I ATCGAT 3 cut(s) 552, 1104, 1400
BsuI GTATCC 2 cut(s) 1373, 1463
BsuRI GGCC 6 cut(s) 60, 322, 352, 746, 823, 1673
BsuTUI ATCGAT 3 cut(s) 552, 1104, 1400
BtgI CCRYGG 2 cut(s) 741, 1674
BtsCI GGATG 7 cut(s) 273, 314, 463, 1272, 1450, 1592, 1654
BtsI GCAGTG 3 cut(s) 1041, 1287, 1578
BveI ACCTGC 1 cut(s) 1399
Cac8I GCNNGC 4 cut(s) 414, 567, 1207, 1578
CaiI CAGNNNCTG 1 cut(s) 1577
CfoI GCGC 1 cut(s) 907
Cfr10I RCCGGY 2 cut(s) 851, 1729
Cfr13I GGNCC 4 cut(s) 101, 200, 320, 821
ClaI ATCGAT 3 cut(s) 552, 1104, 1400
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 2 cut(s) 399, 874
CspAI ACCGGT 1 cut(s) 851
CspCI CAANNNNNGTGG 2 cut(s) 1643, 1678
CviAII CATG 9 cut(s) 119, 284, 413, 426, 814, 1210, 1405, 1626, 1675
CviQI GTAC 2 cut(s) 399, 874
DdeI CTNAG 4 cut(s) 336, 431, 698, 1491
DpnI GATC 6 cut(s) 633, 1165, 1399, 1403, 1548, 1737
DpnII GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
EaeI YGGCCR 2 cut(s) 744, 1671
Eam1104I CTCTTC 1 cut(s) 1121
EarI CTCTTC 1 cut(s) 1121
EciI GGCGGA 1 cut(s) 1748
Ecl136II GAGCTC 2 cut(s) 257, 587
Eco130I CCWWGG 3 cut(s) 93, 207, 1674
Eco24I GRGCYC 2 cut(s) 259, 589
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 101, 200
Eco53kI GAGCTC 2 cut(s) 257, 587
Eco57I CTGAAG 1 cut(s) 1238
Eco91I GGTNACC 1 cut(s) 854
EcoICRI GAGCTC 2 cut(s) 257, 587
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 2 cut(s) 101, 821
EcoO65I GGTNACC 1 cut(s) 854
EcoRII CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
EcoT14I CCWWGG 3 cut(s) 93, 207, 1674
EcoT22I ATGCAT 2 cut(s) 607, 1638
EcoT38I GRGCYC 2 cut(s) 259, 589
ErhI CCWWGG 3 cut(s) 93, 207, 1674
FaeI CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1408, 1629, 1678
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 2 cut(s) 1076, 1204
FatI CATG 9 cut(s) 118, 283, 412, 425, 813, 1209, 1404, 1625, 1674
Fnu4HI GCNGC 7 cut(s) 714, 885, 915, 970, 996, 1345, 1418
FokI GGATG 7 cut(s) 260, 321, 450, 1259, 1437, 1599, 1661
FriOI GRGCYC 2 cut(s) 259, 589
Fsp4HI GCNGC 7 cut(s) 714, 885, 915, 970, 996, 1345, 1418
FspBI CTAG 2 cut(s) 473, 1374
FspI TGCGCA 1 cut(s) 906
GlaI GCGC 1 cut(s) 906
GluI GCNGC 7 cut(s) 714, 885, 915, 970, 996, 1345, 1418
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 6 cut(s) 60, 322, 352, 746, 823, 1673
HapII CCGG 4 cut(s) 681, 852, 871, 1730
HhaI GCGC 1 cut(s) 907
Hin1II CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1408, 1629, 1678
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HincII GTYRAC 2 cut(s) 28, 623
HindII GTYRAC 2 cut(s) 28, 623
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 6 cut(s) 34, 334, 479, 677, 702, 1137
HpaII CCGG 4 cut(s) 681, 852, 871, 1730
HphI GGTGA 9 cut(s) 236, 238, 262, 539, 641, 841, 855, 866, 1048
Hpy166II GTNNAC 7 cut(s) 28, 148, 217, 445, 623, 1654, 1690
Hpy188I TCNGA 3 cut(s) 601, 1070, 1314
Hpy188III TCNNGA 2 cut(s) 559, 947
Hpy8I GTNNAC 7 cut(s) 28, 148, 217, 445, 623, 1654, 1690
Hpy99I CGWCG 1 cut(s) 1318
HpyAV CCTTC 7 cut(s) 77, 389, 499, 527, 704, 1234, 1262
HpyCH4III ACNGT 7 cut(s) 181, 442, 661, 763, 808, 989, 1567
HpyCH4IV ACGT 2 cut(s) 44, 267
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1341, 1414, 1577
HpyF3I CTNAG 4 cut(s) 336, 431, 698, 1491
HpySE526I ACGT 2 cut(s) 44, 267
Hsp92II CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1408, 1629, 1678
HspAI GCGC 1 cut(s) 905
KpnI GGTACC 1 cut(s) 402
Kzo9I GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
LmnI GCTCC 5 cut(s) 198, 360, 449, 584, 592
Lsp1109I GCAGC 5 cut(s) 700, 926, 981, 1007, 1404
LweI GCATC 3 cut(s) 592, 766, 1440
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 2 cut(s) 473, 1374
MaeII ACGT 2 cut(s) 44, 267
MaeIII GTNAC 8 cut(s) 224, 244, 268, 331, 854, 861, 1435, 1640
MalI GATC 6 cut(s) 633, 1165, 1399, 1403, 1548, 1737
MbiI CCGCTC 1 cut(s) 1347
MboI GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
MboII GAAGA 5 cut(s) 512, 727, 1108, 1111, 1454
MfeI CAATTG 3 cut(s) 976, 1125, 1660
MflI RGATCY 2 cut(s) 1163, 1735
MhlI GDGCHC 4 cut(s) 259, 344, 454, 589
MlsI TGGCCA 2 cut(s) 746, 1673
MluNI TGGCCA 2 cut(s) 746, 1673
MlyI GAGTC 5 cut(s) 328, 473, 671, 711, 1131
MmeI TCCRAC 1 cut(s) 1337
Mox20I TGGCCA 2 cut(s) 746, 1673
Mph1103I ATGCAT 2 cut(s) 607, 1638
MscI TGGCCA 2 cut(s) 746, 1673
MseI TTAA 2 cut(s) 845, 1750
MslI CAYNNNNRTG 4 cut(s) 117, 179, 282, 1647
Msp20I TGGCCA 2 cut(s) 746, 1673
MspI CCGG 4 cut(s) 681, 852, 871, 1730
MspR9I CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
MunI CAATTG 3 cut(s) 976, 1125, 1660
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 6 cut(s) 198, 354, 627, 825, 1278, 1620
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1341, 1414, 1577
NciI CCSGG 1 cut(s) 871
NcoI CCATGG 1 cut(s) 1674
NdeII GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
NlaIII CATG 9 cut(s) 122, 287, 416, 429, 817, 1213, 1408, 1629, 1678
NlaIV GGNNCC 4 cut(s) 103, 400, 1165, 1175
NmuCI GTSAC 8 cut(s) 224, 244, 268, 331, 854, 861, 1435, 1640
NsbI TGCGCA 1 cut(s) 906
NsiI ATGCAT 2 cut(s) 607, 1638
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 1 cut(s) 917
PfeI GAWTC 1 cut(s) 34
PflFI GACNNNGTC 2 cut(s) 88, 659
PflMI CCANNNNNTGG 1 cut(s) 279
PinAI ACCGGT 1 cut(s) 851
PkrI GCNGC 7 cut(s) 715, 886, 916, 971, 997, 1346, 1419
PleI GAGTC 5 cut(s) 328, 473, 671, 710, 1131
PpsI GAGTC 5 cut(s) 328, 473, 671, 710, 1131
PpuMI RGGWCCY 1 cut(s) 101
Psp124BI GAGCTC 2 cut(s) 259, 589
Psp5II RGGWCCY 1 cut(s) 101
Psp6I CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
PspEI GGTNACC 1 cut(s) 854
PspGI CCWGG 6 cut(s) 196, 352, 625, 823, 1276, 1618
PspN4I GGNNCC 4 cut(s) 103, 400, 1165, 1175
PspPI GGNCC 4 cut(s) 101, 200, 320, 821
PspPPI RGGWCCY 1 cut(s) 101
PstI CTGCAG 2 cut(s) 1422, 1713
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 2 cut(s) 1163, 1735
PsyI GACNNNGTC 2 cut(s) 88, 659
RsaI GTAC 2 cut(s) 400, 875
RsaNI GTAC 2 cut(s) 399, 874
RseI CAYNNNNRTG 4 cut(s) 117, 179, 282, 1647
SacI GAGCTC 2 cut(s) 259, 589
SaqAI TTAA 2 cut(s) 845, 1750
SatI GCNGC 7 cut(s) 714, 885, 915, 970, 996, 1345, 1418
Sau3AI GATC 6 cut(s) 631, 1163, 1397, 1401, 1546, 1735
Sau96I GGNCC 4 cut(s) 101, 200, 320, 821
SchI GAGTC 5 cut(s) 328, 473, 671, 711, 1131
ScrFI CCNGG 7 cut(s) 198, 354, 627, 825, 871, 1278, 1620
SduI GDGCHC 4 cut(s) 259, 344, 454, 589
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 3 cut(s) 592, 766, 1440
SfcI CTRYAG 3 cut(s) 672, 1418, 1709
SgrAI CRCCGGYG 2 cut(s) 851, 1729
SinI GGWCC 2 cut(s) 101, 200
SmiMI CAYNNNNRTG 4 cut(s) 117, 179, 282, 1647
SmlI CTYRAG 2 cut(s) 559, 646
SmoI CTYRAG 2 cut(s) 559, 646
SphI GCATGC 1 cut(s) 416
SsiI CCGC 6 cut(s) 373, 884, 939, 1050, 1345, 1733
SspMI CTAG 2 cut(s) 473, 1374
SstI GAGCTC 2 cut(s) 259, 589
StyD4I CCNGG 7 cut(s) 196, 352, 625, 823, 869, 1276, 1618
StyI CCWWGG 3 cut(s) 93, 207, 1674
TaaI ACNGT 7 cut(s) 181, 442, 661, 763, 808, 989, 1567
TaiI ACGT 2 cut(s) 47, 270
TaqI TCGA 8 cut(s) 83, 552, 921, 1104, 1323, 1400, 1600, 1719
TauI GCSGC 2 cut(s) 887, 1347
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 2 cut(s) 845, 1750
Tru9I TTAA 2 cut(s) 845, 1750
TseFI GTSAC 8 cut(s) 224, 244, 268, 331, 854, 861, 1435, 1640
TseI GCWGC 5 cut(s) 713, 914, 969, 995, 1417
Tsp45I GTSAC 8 cut(s) 224, 244, 268, 331, 854, 861, 1435, 1640
TspDTI ATGAA 4 cut(s) 69, 452, 1122, 1226
TspGWI ACGGA 3 cut(s) 462, 730, 1482
Tth111I GACNNNGTC 2 cut(s) 88, 659
Van91I CCANNNNNTGG 1 cut(s) 279
VpaK11BI GGWCC 2 cut(s) 101, 200
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 5 cut(s) 108, 326, 1156, 1319, 1504
XceI RCATGY 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 1681
XspI CTAG 2 cut(s) 473, 1374
Zsp2I ATGCAT 2 cut(s) 607, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.