Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001265

Physical Location & Seq

Genomic Coordinates Reverse (-)

30331646 .. 30334278

Gene Structure 2633 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1227800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGGAGGCACTCAACGCCATTTTCGGCAACAAAAACCGATTCTCCTTAAGCCTTTTCAGCCTTTGCCTTCTATTGGCTTCATCGACGATATCCTTGGCAGAACCCAAAATTCACAAGCATGAGTTCGTTATTCAAGCAACAAAGGTGAAGAGGCTGTGCAAAACCCACAACTCCATCACAGTGAATGGACAATACCCTGGACCAACCTTGGAAGTAAACAATGGTGACACTCTTGTCGTCAAAGTAACGAACAAAGCTCGATACAATGCCACCATTCACTGGCATGGGATTAGGCAGATGAGAACTGGATGGGCAGATGGGCCTGAATTTGTGACTCAGTGCCCGATTAGGCCAGGAGGGAGCTACACCTACCGGTTTACAATTCAAGGTCAAGAAGGTACTCTGTGGTGGCATGCTCACAGTTCATGGCTTAGAGCCACCGTTTACGGTGCCCTCATCATTCATCCTAAACAAGGAGACTCCTACCCGTTCACTAAACCGAAACGCGAAACAGCAATTCTTCTTGGTGAATGGTGGGACGCAAACCCTATCGACGTTGTTAGGCAGGCGACTAGGACAGGAGCAGCTCCAAATGTTTCTGATGCATACACCATCAATGGTCAACCTGGTGATCTTTACAATTGTTCAAGCAAAGACACTGTCATAGTCCCTATAGACGTCGGCGAGACCAACCTTCTGAGAGTCATCAACGCTGCCCTCAACCAACCTCTTTTCTTCTCCGTGGCCAACCACAAACTCACCGTTGTTAGTGCTGATGCCTCCTACACCAAACCTTTCACTACCACGGTGCTCATGCTGGGCCCTGGGCAGACCACCGATGTTTTAATTACCGGTGACCAGCCACCAGCCAGGTACTACTTGGCGGCGAGTGCTTATTTCAGTGCTCAAAATGCAGCATTTGACAACACCACCACCACCGCCATTCTTGAATACAGGTCTGCTCCTTGCAGCCCCAATTGCAAAAACGGTCCGACAGTGAAACCCATTATGCCACAACTCCCTGCTTTCAATGACACAAACACTGCTTCTGCCTTCACCAGAAGCTTCAGAAGTCCAAGAAAAGTTGAAGTCCCTACTGAAATCGACGAAAACCTCTTCTTCACAGTCGGTCTTGGACTTAACAACTGCCCTAATAATTTCAGAGCCAGAAGGTGCCAAGGCCCAAACGGGACACGCTTCACTGCCAGCATGAACAATGTGTCATTCGTGTTTCCAACCATCTCAATCCTTCAGGCTTACCAACAAAACATTCCCGGAGTTTTTACTGCCGATTTTCCAGCAAACCCTCCGCTGACATTCGACTACACAGGAAATGTGAGCCGCTCTCTCTGGCAGCCTACGCCTGGCACTAGAGGATACAAGTTGAAGTATGGCTCAAGGGTGCAGATTGTGCTGCAGGACACAAGCATCGTCACACCCGAAAACCATCCTATCCATCTTCACGGATACGATTTTTACATCCTTGCCGAAGGTTTTGGAAATTTCAATGCCCAGAATGACCCGAAAAAATTCAACCTTGTTGATCCACCTATGAGGAACACAGTGGCAGTGCCGGTCAATGGATGGGCAGTCATCCGATTTGTCGCTGACAATCCAGGTGTATGGTTAATGCATTGTCACTTGGATGTTCACATCACCTGGGGTTTGGCCATGGTGTTCTTGGTGGACAATGGAGTTGGGGCACTGCAATCGATCGAGCCACCACCGGCGGATCTGCCCCTGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

583

Amino Acids

64.22

Weight (kDa)

8.68

Isoelectric Point (pI)

32.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	44 - 158	8.9e-45	Multicopper oxidase
Cu-oxidase	PF00394	170 - 320	2e-42	Multicopper oxidase
Cu-oxidase_2	PF07731	431 - 564	1.6e-43	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40370 AT5G05390
fragaria_vesca	FvH4_7g07750 FvH4_7g07800 FvH4_7g25330
malus_domestica	MD01G1158900.v1.1 MD01G1159400.v1.1 MD07G1227800.v1.1 MD07G1227900.v1.1
prunus_persica	Prupe.2G257400_v2.0.a1
pyrus_communis	pycom01g17560 pycom01g17570 pycom07g20630
rosa_chinensis	RchiOBHm_Chr1g0341691 RchiOBHm_Chr1g0341781 RchiOBHm_Chr1g0341901 RchiOBHm_Chr1g0342021 RchiOBHm_Chr1g0342201 RchiOBHm_Chr1g0342281 RchiOBHm_Chr1g0371241
rosa_laevigata	RLG00000026977 RLG00000029124 RLG00000029139 RLG00000029144 RLG00000029148
rosa_multiflora	Rmu_co8157628.1_g000001 Rmu_sc0001400.1_g000014 Rmu_sc0001978.1_g000003 Rmu_sc0002161.1_g000024 Rmu_sc0002161.1_g000035 Rmu_sc0004658.1_g000003
rosa_roxburghii	Rroxscaffold_4G00285850 Rroxscaffold_4G00312320 Rroxscaffold_4G00312390 Rroxscaffold_4G00312700 Rroxscaffold_4G00312750 Rroxscaffold_4G00312770 Rroxscaffold_4G00312800
rosa_rugosa	Rorug01G0152200.1 Rorug01G0152500.1 Rorug01G0153200.1 Rorug01G0153700.1 Rorug01G0362300
rosa_samantha	Rh1BG133800 Rh1BG134300 Rh1BG134600 Rh1BG137000 Rh1BG335900 Rh1CG155200 Rh1CG157900 Rh1CG349300
rosa_wichuraiana	Rw1G013920 Rw1G013930 Rw1G013990 Rw1G032750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	233
AatII	GACGTC	1 cut(s)	681
AccB1I	GGYRCC	2 cut(s)	449, 1173
AccB7I	CCANNNNNTGG	1 cut(s)	279
AccBSI	CCGCTC	1 cut(s)	1344
AccII	CGCG	1 cut(s)	507
AciI	CCGC	5 cut(s)	884, 939, 1310, 1342, 1730
AclWI	GGATC	2 cut(s)	1538, 1740
AcoI	YGGCCR	2 cut(s)	744, 1668
AcsI	RAATTY	4 cut(s)	108, 326, 1501, 1529
AcuI	CTGAAG	2 cut(s)	1051, 1235
AcyI	GRCGYC	1 cut(s)	678
AfaI	GTAC	2 cut(s)	400, 875
AfiI	CCNNNNNNNGG	5 cut(s)	73, 279, 473, 1364, 1580
AflII	CTTAAG	1 cut(s)	46
AgeI	ACCGGT	2 cut(s)	372, 851
AgsI	TTSAA	9 cut(s)	134, 386, 648, 950, 1030, 1088, 1387, 1507, 1534
AjnI	CCWGG	8 cut(s)	196, 352, 625, 823, 869, 1363, 1615, 1658
AjuI	GAANNNNNNNTTGG	2 cut(s)	1254, 1286
AluBI	AGCT	4 cut(s)	257, 363, 587, 1065
AluI	AGCT	4 cut(s)	257, 363, 587, 1065
Alw21I	GWGCWC	2 cut(s)	813, 907
Alw26I	GTCTC	2 cut(s)	471, 680
AlwI	GGATC	2 cut(s)	1538, 1740
AoxI	GGCC	6 cut(s)	320, 350, 744, 820, 1180, 1668
ApaI	GGGCCC	1 cut(s)	824
ApeKI	GCWGC	6 cut(s)	584, 713, 914, 969, 1354, 1414
ApoI	RAATTY	4 cut(s)	108, 326, 1501, 1529
ArsI	GACNNNNNNTTYG	2 cut(s)	645, 677
AsiGI	ACCGGT	2 cut(s)	372, 851
Asp700I	GAANNNNTTC	1 cut(s)	1529
AspS9I	GGNCC	6 cut(s)	200, 320, 820, 821, 989, 1181
AsuC2I	CCSGG	1 cut(s)	1275
AsuHPI	GGTGA	8 cut(s)	157, 236, 539, 641, 751, 866, 1048, 1648
AvaII	GGWCC	2 cut(s)	200, 989
BaeGI	GKGCMC	4 cut(s)	344, 454, 824, 1705
BalI	TGGCCA	2 cut(s)	746, 1670
BanI	GGYRCC	2 cut(s)	449, 1173
BanII	GRGCYC	1 cut(s)	824
Bbv12I	GWGCWC	2 cut(s)	813, 907
BbvI	GCAGC	6 cut(s)	596, 700, 926, 981, 1366, 1401
BccI	CCATC	8 cut(s)	182, 303, 311, 620, 1247, 1455, 1464, 1578
BcgI	CGANNNNNNTGC	2 cut(s)	1692, 1726
BciT130I	CCWGG	8 cut(s)	198, 354, 627, 825, 871, 1365, 1617, 1660
BciVI	GTATCC	2 cut(s)	1370, 1460
BcnI	CCSGG	1 cut(s)	1275
BcoDI	GTCTC	2 cut(s)	471, 680
BfaI	CTAG	2 cut(s)	573, 1371
BfmI	CTRYAG	2 cut(s)	672, 1415
BfrI	CTTAAG	1 cut(s)	46
BfuI	GTATCC	2 cut(s)	1370, 1460
BisI	GCNGC	8 cut(s)	585, 714, 885, 915, 970, 1342, 1355, 1415
BlsI	GCNGC	8 cut(s)	586, 715, 886, 916, 971, 1343, 1356, 1416
Bme1390I	CCNGG	9 cut(s)	198, 354, 627, 825, 871, 1275, 1365, 1617, 1660
Bme18I	GGWCC	2 cut(s)	200, 989
BmgT120I	GGNCC	6 cut(s)	200, 320, 820, 821, 989, 1181
BmiI	GGNNCC	3 cut(s)	451, 822, 1175
BmrFI	CCNGG	9 cut(s)	198, 354, 627, 825, 871, 1275, 1365, 1617, 1660
BmsI	GCATC	3 cut(s)	592, 766, 1437
BplI	GAGNNNNNCTC	2 cut(s)	1330, 1362
BpuEI	CTTGAG	1 cut(s)	1381
BpuMI	CCSGG	1 cut(s)	1275
Bsa29I	ATCGAT	1 cut(s)	1712
BsaHI	GRCGYC	1 cut(s)	678
BsaI	GGTCTC	1 cut(s)	680
BsaWI	WCCGGW	2 cut(s)	372, 851
BsaXI	ACNNNNNCTCC	2 cut(s)	26, 56
Bsc4I	CCNNNNNNNGG	5 cut(s)	73, 279, 473, 1364, 1580
Bse118I	RCCGGY	4 cut(s)	372, 851, 1573, 1726
Bse1I	ACTGG	2 cut(s)	284, 310
BseBI	CCWGG	8 cut(s)	198, 354, 627, 825, 871, 1365, 1617, 1660
BseCI	ATCGAT	1 cut(s)	1712
BseGI	GGATG	7 cut(s)	314, 463, 1447, 1479, 1589, 1593, 1651
BseLI	CCNNNNNNNGG	5 cut(s)	73, 279, 473, 1364, 1580
BseMII	CTCAG	2 cut(s)	350, 689
BseNI	ACTGG	2 cut(s)	284, 310
BseSI	GKGCMC	4 cut(s)	344, 454, 824, 1705
BseXI	GCAGC	6 cut(s)	596, 700, 926, 981, 1366, 1401
BseYI	CCCAGC	1 cut(s)	817
BsgI	GTGCAG	1 cut(s)	1424
Bsh1236I	CGCG	1 cut(s)	507
Bsh1285I	CGRYCG	1 cut(s)	1716
BshFI	GGCC	6 cut(s)	322, 352, 746, 822, 1182, 1670
BshNI	GGYRCC	2 cut(s)	449, 1173
BshTI	ACCGGT	2 cut(s)	372, 851
BshVI	ATCGAT	1 cut(s)	1712
BsiEI	CGRYCG	1 cut(s)	1716
BsiHKAI	GWGCWC	2 cut(s)	813, 907
BsiSI	CCGG	5 cut(s)	373, 852, 1275, 1574, 1727
BslFI	GGGAC	4 cut(s)	551, 653, 1076, 1204
BslI	CCNNNNNNNGG	5 cut(s)	73, 279, 473, 1364, 1580
BsmAI	GTCTC	2 cut(s)	471, 680
BsmFI	GGGAC	4 cut(s)	551, 653, 1076, 1204
BsnI	GGCC	6 cut(s)	322, 352, 746, 822, 1182, 1670
Bso31I	GGTCTC	1 cut(s)	680
Bsp120I	GGGCCC	1 cut(s)	820
Bsp1286I	GDGCHC	6 cut(s)	344, 454, 813, 824, 907, 1705
Bsp143I	GATC	4 cut(s)	631, 1543, 1713, 1732
Bsp19I	CCATGG	1 cut(s)	1671
BspACI	CCGC	5 cut(s)	884, 939, 1310, 1342, 1730
BspANI	GGCC	6 cut(s)	322, 352, 746, 822, 1182, 1670
BspCNI	CTCAG	2 cut(s)	349, 690
BspDI	ATCGAT	1 cut(s)	1712
BspFNI	CGCG	1 cut(s)	507
BspLI	GGNNCC	3 cut(s)	451, 822, 1175
BspMAI	CTGCAG	1 cut(s)	1419
BspPI	GGATC	2 cut(s)	1538, 1740
BspT107I	GGYRCC	2 cut(s)	449, 1173
BspTI	CTTAAG	1 cut(s)	46
BspTNI	GGTCTC	1 cut(s)	680
BsrBI	CCGCTC	1 cut(s)	1344
BsrFI	RCCGGY	4 cut(s)	372, 851, 1573, 1726
BsrI	ACTGG	2 cut(s)	284, 310
BssAI	RCCGGY	4 cut(s)	372, 851, 1573, 1726
BssMI	GATC	4 cut(s)	631, 1543, 1713, 1732
BssNI	GRCGYC	1 cut(s)	678
BssT1I	CCWWGG	4 cut(s)	93, 207, 1177, 1671
Bst2UI	CCWGG	8 cut(s)	198, 354, 627, 825, 871, 1365, 1617, 1660
Bst6I	CTCTTC	2 cut(s)	143, 1121
BstACI	GRCGYC	1 cut(s)	678
BstAFI	CTTAAG	1 cut(s)	46
BstAPI	GCANNNNNTGC	1 cut(s)	1411
BstC8I	GCNNGC	3 cut(s)	414, 567, 1207
BstDEI	CTNAG	3 cut(s)	336, 431, 698
BstDSI	CCRYGG	3 cut(s)	741, 804, 1671
BstEII	GGTNACC	1 cut(s)	854
BstENI	CCTNNNNNAGG	1 cut(s)	471
BstF5I	GGATG	7 cut(s)	314, 463, 1447, 1479, 1589, 1593, 1651
BstFNI	CGCG	1 cut(s)	507
BstKTI	GATC	4 cut(s)	634, 1546, 1716, 1735
BstMAI	GTCTC	2 cut(s)	471, 680
BstMBI	GATC	4 cut(s)	631, 1543, 1713, 1732
BstMCI	CGRYCG	1 cut(s)	1716
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 57, 890, 911, 978, 1360, 1411
BstNI	CCWGG	8 cut(s)	198, 354, 627, 825, 871, 1365, 1617, 1660
BstNSI	RCATGY	1 cut(s)	416
BstPI	GGTNACC	1 cut(s)	854
BstSCI	CCNGG	9 cut(s)	196, 352, 625, 823, 869, 1273, 1363, 1615, 1658
BstSFI	CTRYAG	2 cut(s)	672, 1415
BstSLI	GKGCMC	4 cut(s)	344, 454, 824, 1705
BstUI	CGCG	1 cut(s)	507
BstV1I	GCAGC	6 cut(s)	596, 700, 926, 981, 1366, 1401
BstX2I	RGATCY	1 cut(s)	1732
BstXI	CCANNNNNNTGG	1 cut(s)	1623
BstYI	RGATCY	1 cut(s)	1732
Bsu15I	ATCGAT	1 cut(s)	1712
BsuI	GTATCC	2 cut(s)	1370, 1460
BsuRI	GGCC	6 cut(s)	322, 352, 746, 822, 1182, 1670
BsuTUI	ATCGAT	1 cut(s)	1712
BtgI	CCRYGG	3 cut(s)	741, 804, 1671
BtsCI	GGATG	7 cut(s)	314, 463, 1447, 1479, 1589, 1593, 1651
BtsI	GCAGTG	4 cut(s)	1041, 1200, 1575, 1703
Cac8I	GCNNGC	3 cut(s)	414, 567, 1207
Cfr10I	RCCGGY	4 cut(s)	372, 851, 1573, 1726
Cfr13I	GGNCC	6 cut(s)	200, 320, 820, 821, 989, 1181
ClaI	ATCGAT	1 cut(s)	1712
CpoI	CGGWCCG	1 cut(s)	989
CseI	GACGC	1 cut(s)	548
CsiI	ACCWGGT	1 cut(s)	625
Csp6I	GTAC	2 cut(s)	399, 874
CspAI	ACCGGT	2 cut(s)	372, 851
CspI	CGGWCCG	1 cut(s)	989
CviAII	CATG	7 cut(s)	119, 284, 413, 426, 814, 1210, 1672
CviQI	GTAC	2 cut(s)	399, 874
DdeI	CTNAG	3 cut(s)	336, 431, 698
DpnI	GATC	4 cut(s)	633, 1545, 1715, 1734
DpnII	GATC	4 cut(s)	631, 1543, 1713, 1732
DrdI	GACNNNNNNGTC	1 cut(s)	233
DseDI	GACNNNNNNGTC	1 cut(s)	233
EaeI	YGGCCR	2 cut(s)	744, 1668
Eam1104I	CTCTTC	2 cut(s)	143, 1121
EarI	CTCTTC	2 cut(s)	143, 1121
EciI	GGCGGA	1 cut(s)	1745
Eco130I	CCWWGG	4 cut(s)	93, 207, 1177, 1671
Eco24I	GRGCYC	1 cut(s)	824
Eco31I	GGTCTC	1 cut(s)	680
Eco32I	GATATC	1 cut(s)	90
Eco47I	GGWCC	2 cut(s)	200, 989
Eco57I	CTGAAG	2 cut(s)	1051, 1235
Eco91I	GGTNACC	1 cut(s)	854
EcoNI	CCTNNNNNAGG	1 cut(s)	471
EcoO109I	RGGNCCY	1 cut(s)	821
EcoO65I	GGTNACC	1 cut(s)	854
EcoRII	CCWGG	8 cut(s)	196, 352, 625, 823, 869, 1363, 1615, 1658
EcoRV	GATATC	1 cut(s)	90
EcoT14I	CCWWGG	4 cut(s)	93, 207, 1177, 1671
EcoT22I	ATGCAT	2 cut(s)	607, 1635
EcoT38I	GRGCYC	1 cut(s)	824
ErhI	CCWWGG	4 cut(s)	93, 207, 1177, 1671
FaeI	CATG	7 cut(s)	122, 287, 416, 429, 817, 1213, 1675
FaqI	GGGAC	4 cut(s)	551, 653, 1076, 1204
FatI	CATG	7 cut(s)	118, 283, 412, 425, 813, 1209, 1671
Fnu4HI	GCNGC	8 cut(s)	585, 714, 885, 915, 970, 1342, 1355, 1415
FokI	GGATG	7 cut(s)	321, 450, 1434, 1466, 1580, 1596, 1658
FriOI	GRGCYC	1 cut(s)	824
Fsp4HI	GCNGC	8 cut(s)	585, 714, 885, 915, 970, 1342, 1355, 1415
FspBI	CTAG	2 cut(s)	573, 1371
GluI	GCNGC	8 cut(s)	585, 714, 885, 915, 970, 1342, 1355, 1415
GsaI	CCCAGC	1 cut(s)	821
HaeIII	GGCC	6 cut(s)	322, 352, 746, 822, 1182, 1670
HapII	CCGG	5 cut(s)	373, 852, 1275, 1574, 1727
HgaI	GACGC	1 cut(s)	548
Hin1I	GRCGYC	1 cut(s)	678
Hin1II	CATG	7 cut(s)	122, 287, 416, 429, 817, 1213, 1675
HincII	GTYRAC	1 cut(s)	623
HindII	GTYRAC	1 cut(s)	623
HindIII	AAGCTT	1 cut(s)	1063
HinfI	GANTC	4 cut(s)	39, 334, 479, 702
HpaII	CCGG	5 cut(s)	373, 852, 1275, 1574, 1727
HphI	GGTGA	8 cut(s)	157, 236, 539, 641, 751, 866, 1048, 1648
Hpy166II	GTNNAC	7 cut(s)	217, 378, 445, 492, 623, 1651, 1687
Hpy188I	TCNGA	6 cut(s)	601, 699, 993, 1070, 1163, 1598
Hpy188III	TCNNGA	2 cut(s)	392, 947
Hpy8I	GTNNAC	7 cut(s)	217, 378, 445, 492, 623, 1651, 1687
Hpy99I	CGWCG	4 cut(s)	88, 557, 683, 1109
HpyAV	CCTTC	7 cut(s)	77, 389, 704, 1063, 1164, 1259, 1484
HpyCH4IV	ACGT	2 cut(s)	555, 678
HpyCH4V	TGCA	9 cut(s)	159, 605, 914, 969, 981, 1405, 1417, 1633, 1708
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 57, 890, 911, 978, 1360, 1411
HpyF3I	CTNAG	3 cut(s)	336, 431, 698
HpySE526I	ACGT	2 cut(s)	555, 678
Hsp92I	GRCGYC	1 cut(s)	678
Hsp92II	CATG	7 cut(s)	122, 287, 416, 429, 817, 1213, 1675
Kzo9I	GATC	4 cut(s)	631, 1543, 1713, 1732
LmnI	GCTCC	4 cut(s)	360, 581, 592, 967
Lsp1109I	GCAGC	6 cut(s)	596, 700, 926, 981, 1366, 1401
LweI	GCATC	3 cut(s)	592, 766, 1437
MabI	ACCWGGT	1 cut(s)	625
MaeI	CTAG	2 cut(s)	573, 1371
MaeII	ACGT	2 cut(s)	555, 678
MaeIII	GTNAC	6 cut(s)	224, 244, 331, 854, 1432, 1637
MalI	GATC	4 cut(s)	633, 1545, 1715, 1734
MbiI	CCGCTC	1 cut(s)	1344
MboI	GATC	4 cut(s)	631, 1543, 1713, 1732
MboII	GAAGA	6 cut(s)	160, 512, 727, 1108, 1111, 1451
MfeI	CAATTG	2 cut(s)	640, 976
MflI	RGATCY	1 cut(s)	1732
MhlI	GDGCHC	6 cut(s)	344, 454, 813, 824, 907, 1705
MlsI	TGGCCA	2 cut(s)	746, 1670
MluNI	TGGCCA	2 cut(s)	746, 1670
MlyI	GAGTC	3 cut(s)	328, 473, 711
MmeI	TCCRAC	2 cut(s)	1016, 1259
Mox20I	TGGCCA	2 cut(s)	746, 1670
Mph1103I	ATGCAT	2 cut(s)	607, 1635
MroXI	GAANNNNTTC	1 cut(s)	1529
MscI	TGGCCA	2 cut(s)	746, 1670
MseI	TTAA	5 cut(s)	47, 845, 1140, 1628, 1747
MslI	CAYNNNNRTG	4 cut(s)	117, 179, 282, 1644
Msp20I	TGGCCA	2 cut(s)	746, 1670
MspA1I	CMGCKG	1 cut(s)	1312
MspCI	CTTAAG	1 cut(s)	46
MspI	CCGG	5 cut(s)	373, 852, 1275, 1574, 1727
MspR9I	CCNGG	9 cut(s)	198, 354, 627, 825, 871, 1275, 1365, 1617, 1660
MunI	CAATTG	2 cut(s)	640, 976
MvaI	CCWGG	8 cut(s)	198, 354, 627, 825, 871, 1365, 1617, 1660
MvnI	CGCG	1 cut(s)	507
MwoI	GCNNNNNNNGC	7 cut(s)	14, 57, 890, 911, 978, 1360, 1411
NciI	CCSGG	1 cut(s)	1275
NcoI	CCATGG	1 cut(s)	1671
NdeII	GATC	4 cut(s)	631, 1543, 1713, 1732
NlaIII	CATG	7 cut(s)	122, 287, 416, 429, 817, 1213, 1675
NlaIV	GGNNCC	3 cut(s)	451, 822, 1175
NmuCI	GTSAC	5 cut(s)	224, 331, 854, 1432, 1637
NsiI	ATGCAT	2 cut(s)	607, 1635
NspI	RCATGY	1 cut(s)	416
PaeI	GCATGC	1 cut(s)	416
PasI	CCCWGGG	1 cut(s)	824
PcsI	WCGNNNNNNNCGW	1 cut(s)	1437
PdmI	GAANNNNTTC	1 cut(s)	1529
PfeI	GAWTC	1 cut(s)	39
PflFI	GACNNNGTC	1 cut(s)	659
PflMI	CCANNNNNTGG	1 cut(s)	279
PfoI	TCCNGGA	1 cut(s)	1273
PinAI	ACCGGT	2 cut(s)	372, 851
PkrI	GCNGC	8 cut(s)	586, 715, 886, 916, 971, 1343, 1356, 1416
Ple19I	CGATCG	1 cut(s)	1716
PleI	GAGTC	3 cut(s)	328, 473, 710
PpsI	GAGTC	3 cut(s)	328, 473, 710
Psp6I	CCWGG	8 cut(s)	196, 352, 625, 823, 869, 1363, 1615, 1658
PspEI	GGTNACC	1 cut(s)	854
PspFI	CCCAGC	1 cut(s)	817
PspGI	CCWGG	8 cut(s)	196, 352, 625, 823, 869, 1363, 1615, 1658
PspN4I	GGNNCC	3 cut(s)	451, 822, 1175
PspOMI	GGGCCC	1 cut(s)	820
PspPI	GGNCC	6 cut(s)	200, 320, 820, 821, 989, 1181
PstI	CTGCAG	1 cut(s)	1419
PsuI	RGATCY	1 cut(s)	1732
PsyI	GACNNNGTC	1 cut(s)	659
PvuI	CGATCG	1 cut(s)	1716
RsaI	GTAC	2 cut(s)	400, 875
RsaNI	GTAC	2 cut(s)	399, 874
RseI	CAYNNNNRTG	4 cut(s)	117, 179, 282, 1644
Rsr2I	CGGWCCG	1 cut(s)	989
RsrII	CGGWCCG	1 cut(s)	989
SaqAI	TTAA	5 cut(s)	47, 845, 1140, 1628, 1747
SatI	GCNGC	8 cut(s)	585, 714, 885, 915, 970, 1342, 1355, 1415
Sau3AI	GATC	4 cut(s)	631, 1543, 1713, 1732
Sau96I	GGNCC	6 cut(s)	200, 320, 820, 821, 989, 1181
SchI	GAGTC	3 cut(s)	328, 473, 711
ScrFI	CCNGG	9 cut(s)	198, 354, 627, 825, 871, 1275, 1365, 1617, 1660
SduI	GDGCHC	6 cut(s)	344, 454, 813, 824, 907, 1705
SexAI	ACCWGGT	1 cut(s)	625
SfaNI	GCATC	3 cut(s)	592, 766, 1437
SfcI	CTRYAG	2 cut(s)	672, 1415
SgrAI	CRCCGGYG	1 cut(s)	1726
SinI	GGWCC	2 cut(s)	200, 989
SmiMI	CAYNNNNRTG	4 cut(s)	117, 179, 282, 1644
SmlI	CTYRAG	2 cut(s)	46, 1396
SmoI	CTYRAG	2 cut(s)	46, 1396
SphI	GCATGC	1 cut(s)	416
SsiI	CCGC	5 cut(s)	884, 939, 1310, 1342, 1730
SspMI	CTAG	2 cut(s)	573, 1371
StyD4I	CCNGG	9 cut(s)	196, 352, 625, 823, 869, 1273, 1363, 1615, 1658
StyI	CCWWGG	4 cut(s)	93, 207, 1177, 1671
TaiI	ACGT	2 cut(s)	558, 681
TaqI	TCGA	7 cut(s)	83, 259, 552, 1104, 1320, 1712, 1716
TaqII	GACCGA	1 cut(s)	1118
TauI	GCSGC	2 cut(s)	887, 1344
TfiI	GAWTC	1 cut(s)	39
Tru1I	TTAA	5 cut(s)	47, 845, 1140, 1628, 1747
Tru9I	TTAA	5 cut(s)	47, 845, 1140, 1628, 1747
TseFI	GTSAC	5 cut(s)	224, 331, 854, 1432, 1637
TseI	GCWGC	6 cut(s)	584, 713, 914, 969, 1354, 1414
Tsp45I	GTSAC	5 cut(s)	224, 331, 854, 1432, 1637
TspDTI	ATGAA	4 cut(s)	69, 414, 452, 1226
TspGWI	ACGGA	2 cut(s)	730, 1479
Tth111I	GACNNNGTC	1 cut(s)	659
Van91I	CCANNNNNTGG	1 cut(s)	279
Vha464I	CTTAAG	1 cut(s)	46
VpaK11BI	GGWCC	2 cut(s)	200, 989
XagI	CCTNNNNNAGG	1 cut(s)	471
XapI	RAATTY	4 cut(s)	108, 326, 1501, 1529
XceI	RCATGY	1 cut(s)	416
XcmI	CCANNNNNNNNNTGG	2 cut(s)	877, 1678
XmnI	GAANNNNTTC	1 cut(s)	1529
XspI	CTAG	2 cut(s)	573, 1371
ZraI	GACGTC	1 cut(s)	679
Zsp2I	ATGCAT	2 cut(s)	607, 1635

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.