pycom01g17560

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001265
Physical Location & Seq
Reverse (-)
16807464 .. 16809735
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17560.2

Sequence Viewer

Length: 1773 bp
ATGGAGGTACTCAACGCCATTTGCAGCAACAAAATCCGACTCTCCTTATGCCTTTTCGGCCTTTGCCTCTTATTGGCTTCATCGACAATGTGTTTGGCAGAACCCAAAACTCACCAGCATGAGTTTGTTATTCAAGCAACAAAAGTGAAGAGGCTGTGCAAAACCCACAACTCCATCACAGTAAATGGACAGTACCCTGGACCAACCTTGGAAGTAAACAATGGTGACACTCTTGTCATCAAAGTTACAAACAAAGCTCGATACAATGTCACTATTCACTGGCCTTATCTTCTTTATTTCAGGCATGGGATTAGGCAGATTAGAACTGGATGGGCAGATGGGCCAGAATTTGTGACTCAGTGCCCGATTAGGCCAGGAGGAAGCTATACCTACCGATTTACAATTCAAGGGCAAGAAGGTACTCTCTGGTGGCATGCTCACAGTTCATGGCTTAGAGCCACCGTTTACGGCGCACTCATCATTCATCCTAAACAAGGAGACTCCTACCCCTTCACTAAACCGAAACGCGAAACAACCCTTCTTCTTGGTGAATGGTGGGATGCTAACCCTATCGATGTTATAAGGCAGGCTACTAGGACAGGAGGAGCTCCAAATGTTTCTGATGCATACACCATCAATGGTCAACCTGGCGATCTTTACAATTGCTCAAGCAAAGACACTGTCATAGTTCCTATAGACACCGGCGAGACCAACCTTCTTAGAGTCATCAATGCTGCCCTCAACCAACCTCTTTTCTTCTCCGTGGCCAACCACAAACTCACTGTTGTTAGTGCTGATGCCTCCTACACCAAACCTTTCACCACCACGGTACTCATGCTAGGCCCTGGGCAGACCACCGATGTTTTAATCACGGGTGACCAGCCACCAGCCCGGTACTACTTAGCGGCGAGTGCTTATTTCAGCGCGCAAAATGCAGCATTCGACAACACCACCACCACCGCCATTCTTGAATACAGGTCCGCCCCTTGCAGCCCCAGTTGCAAAAACGGTCCAACAGCCAAACCGATTATGCCACAACTCCCTGCTTTCAATGACACAAACACTGCTTCTGCTTTCACCAAGAGTTTCAGAAGTCCAAGAAAAGTTAAAGTCCCAACTGAAATCGACGAAAACCTCTTCTTCACAATCGGTCTTGGACTCAACAATTGCCCTAAACATTTCAGAGCCAGAAGGTGCCAAGGCCCAAACGGGACACGCTTCACTGCCAGCATGAACAATGTGTCCTTCGTGCTTCCAAACAACATGTCAATCCTTCAGGCTTACCAACAAAACATCCCTGGAGTTTTCACCGCTGATTTTCCAGCAAACCCTTCGAAGAAATTCGACTACACGGGCAATGTGAGCCGTTCTCTATGGCAGCCTTTGTCGGGCACTAGGGGATACAACTTGAAGTATGGATCAAGGGTGCAGGTTGTGCTGCAGGACACAAGCATCGTCACACCCGAAAACCATCCTATCCATCTTCACGGATACGATTTTTACATCCTTGCCGAAGGTTTTGGAAATTTCAATGCCAAGACCGATACCCAAAAGTTTAACCTTATTGATCCACCTATGAGGAACACAGTGGCAGTGCCCGCGAATGGATGGGCAGTTATCCGATTCGTCGCTGACAATCCAGGTGCATGGATAATGCATTGTCACTTGGATGTTCACATCAACTGGGGTTTGGCCATGGTGTTCTTGGTGGACAATGGAGTTGGGGAACTGCAAACGATGGAGCCACCACCAGTGGATCTGCCTCTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

591

Amino Acids

65.38

Weight (kDa)

8.82

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 581
AasI GACNNNNNNGTC 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 1420
AccB1I GGYRCC 1 cut(s) 1194
AccII CGCG 3 cut(s) 528, 926, 1601
AciI CCGC 5 cut(s) 905, 960, 981, 1311, 1599
AclWI GGATC 3 cut(s) 1426, 1562, 1764
AcoI YGGCCR 2 cut(s) 765, 1692
AcsI RAATTY 3 cut(s) 347, 1340, 1525
AcuI CTGAAG 1 cut(s) 1259
AfaI GTAC 5 cut(s) 9, 194, 421, 831, 896
AfiI CCNNNNNNNGG 4 cut(s) 73, 494, 1388, 1604
AflIII ACRYGT 1 cut(s) 1263
AgsI TTSAA 6 cut(s) 134, 407, 971, 1051, 1411, 1531
AjnI CCWGG 6 cut(s) 196, 373, 646, 844, 1297, 1639
AloI GAACNNNNNNTCC 2 cut(s) 1226, 1258
AluBI AGCT 3 cut(s) 257, 384, 608
AluI AGCT 3 cut(s) 257, 384, 608
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 2 cut(s) 492, 701
AlwI GGATC 3 cut(s) 1426, 1562, 1764
AoxI GGCC 8 cut(s) 58, 281, 341, 371, 765, 841, 1201, 1692
ApeKI GCWGC 6 cut(s) 24, 734, 935, 990, 1378, 1438
ApoI RAATTY 3 cut(s) 347, 1340, 1525
ArsI GACNNNNNNTTYG 6 cut(s) 76, 108, 666, 698, 1250, 1282
Asp700I GAANNNNTTC 1 cut(s) 1340
AspLEI GCGC 3 cut(s) 473, 926, 928
AspS9I GGNCC 6 cut(s) 200, 341, 842, 978, 1010, 1202
AsuC2I CCSGG 1 cut(s) 892
AsuHPI GGTGA 7 cut(s) 104, 236, 560, 811, 887, 1069, 1300
AsuII TTCGAA 1 cut(s) 1334
AvaII GGWCC 3 cut(s) 200, 978, 1010
BaeGI GKGCMC 3 cut(s) 365, 1394, 1599
BalI TGGCCA 2 cut(s) 767, 1694
BanI GGYRCC 1 cut(s) 1194
BanII GRGCYC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 6 cut(s) 36, 721, 947, 1002, 1390, 1425
BccI CCATC 8 cut(s) 182, 324, 332, 641, 1479, 1488, 1602, 1732
BceAI ACGGC 2 cut(s) 484, 1350
BcgI CGANNNNNNTGC 2 cut(s) 1314, 1348
BciT130I CCWGG 6 cut(s) 198, 375, 648, 846, 1299, 1641
BciVI GTATCC 2 cut(s) 1394, 1484
BcnI CCSGG 1 cut(s) 892
BcoDI GTCTC 2 cut(s) 492, 701
BfaI CTAG 3 cut(s) 594, 839, 1395
BfmI CTRYAG 2 cut(s) 693, 1439
BfuAI ACCTGC 1 cut(s) 1420
BfuI GTATCC 2 cut(s) 1394, 1484
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 7 cut(s) 25, 735, 906, 936, 991, 1379, 1439
BlsI GCNGC 7 cut(s) 26, 736, 907, 937, 992, 1380, 1440
Bme1390I CCNGG 7 cut(s) 198, 375, 648, 846, 892, 1299, 1641
Bme18I GGWCC 3 cut(s) 200, 978, 1010
BmgT120I GGNCC 6 cut(s) 200, 341, 842, 978, 1010, 1202
BmiI GGNNCC 2 cut(s) 1196, 1743
BmrFI CCNGG 7 cut(s) 198, 375, 648, 846, 892, 1299, 1641
BmrI ACTGGG 2 cut(s) 990, 1693
BmsI GCATC 4 cut(s) 550, 613, 787, 1461
BmuI ACTGGG 2 cut(s) 990, 1693
BplI GAGNNNNNCTC 2 cut(s) 1354, 1386
BpmI CTGGAG 1 cut(s) 1320
Bpu14I TTCGAA 1 cut(s) 1334
BpuEI CTTGAG 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 892
Bsa29I ATCGAT 1 cut(s) 573
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 9 cut(s) 196, 207, 762, 825, 844, 845, 1198, 1297, 1695
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 494, 1388, 1604
Bse118I RCCGGY 1 cut(s) 701
Bse1I ACTGG 5 cut(s) 284, 331, 996, 1688, 1751
Bse3DI GCAATG 1 cut(s) 1363
BseBI CCWGG 6 cut(s) 198, 375, 648, 846, 1299, 1641
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 9 cut(s) 196, 207, 762, 825, 844, 845, 1198, 1297, 1695
BseGI GGATG 8 cut(s) 335, 484, 565, 1293, 1471, 1503, 1613, 1675
BseLI CCNNNNNNNGG 4 cut(s) 73, 494, 1388, 1604
BseMI GCAATG 1 cut(s) 1363
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 5 cut(s) 284, 331, 996, 1688, 1751
BsePI GCGCGC 1 cut(s) 924
BseRI GAGGAG 1 cut(s) 618
BseSI GKGCMC 3 cut(s) 365, 1394, 1599
BseXI GCAGC 6 cut(s) 36, 721, 947, 1002, 1390, 1425
BsgI GTGCAG 1 cut(s) 1448
Bsh1236I CGCG 3 cut(s) 528, 926, 1601
BshFI GGCC 8 cut(s) 60, 283, 343, 373, 767, 843, 1203, 1694
BshNI GGYRCC 1 cut(s) 1194
BshVI ATCGAT 1 cut(s) 573
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 2 cut(s) 702, 892
BslFI GGGAC 2 cut(s) 1097, 1225
BslI CCNNNNNNNGG 4 cut(s) 73, 494, 1388, 1604
BsmAI GTCTC 2 cut(s) 492, 701
BsmFI GGGAC 2 cut(s) 1097, 1225
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 8 cut(s) 60, 283, 343, 373, 767, 843, 1203, 1694
Bso31I GGTCTC 1 cut(s) 701
Bsp119I TTCGAA 1 cut(s) 1334
Bsp1286I GDGCHC 4 cut(s) 365, 610, 1394, 1599
Bsp143I GATC 4 cut(s) 652, 1418, 1567, 1756
Bsp19I CCATGG 1 cut(s) 1695
BspACI CCGC 5 cut(s) 905, 960, 981, 1311, 1599
BspANI GGCC 8 cut(s) 60, 283, 343, 373, 767, 843, 1203, 1694
BspCNI CTCAG 1 cut(s) 370
BspDI ATCGAT 1 cut(s) 573
BspFNI CGCG 3 cut(s) 528, 926, 1601
BspLI GGNNCC 2 cut(s) 1196, 1743
BspMAI CTGCAG 1 cut(s) 1443
BspMI ACCTGC 1 cut(s) 1420
BspPI GGATC 3 cut(s) 1426, 1562, 1764
BspT104I TTCGAA 1 cut(s) 1334
BspT107I GGYRCC 1 cut(s) 1194
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 1363
BsrFI RCCGGY 1 cut(s) 701
BsrI ACTGG 5 cut(s) 284, 331, 996, 1688, 1751
BssAI RCCGGY 1 cut(s) 701
BssECI CCNNGG 9 cut(s) 196, 207, 762, 825, 844, 845, 1198, 1297, 1695
BssHII GCGCGC 1 cut(s) 924
BssMI GATC 4 cut(s) 652, 1418, 1567, 1756
BssT1I CCWWGG 3 cut(s) 207, 1198, 1695
Bst2UI CCWGG 6 cut(s) 198, 375, 648, 846, 1299, 1641
Bst4CI ACNGT 9 cut(s) 181, 192, 443, 463, 682, 784, 829, 1010, 1588
Bst6I CTCTTC 2 cut(s) 143, 1142
BstAPI GCANNNNNTGC 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 1334
BstC8I GCNNGC 5 cut(s) 435, 588, 926, 1228, 1599
BstDEI CTNAG 4 cut(s) 357, 452, 719, 901
BstDSI CCRYGG 3 cut(s) 762, 825, 1695
BstEII GGTNACC 1 cut(s) 875
BstENI CCTNNNNNAGG 1 cut(s) 492
BstF5I GGATG 8 cut(s) 335, 484, 565, 1293, 1471, 1503, 1613, 1675
BstFNI CGCG 3 cut(s) 528, 926, 1601
BstHHI GCGC 3 cut(s) 473, 926, 928
BstKTI GATC 4 cut(s) 655, 1421, 1570, 1759
BstMAI GTCTC 2 cut(s) 492, 701
BstMBI GATC 4 cut(s) 652, 1418, 1567, 1756
BstMWI GCNNNNNNNGC 7 cut(s) 57, 911, 932, 999, 1362, 1435, 1598
BstNI CCWGG 6 cut(s) 198, 375, 648, 846, 1299, 1641
BstNSI RCATGY 2 cut(s) 437, 1267
BstPI GGTNACC 1 cut(s) 875
BstSCI CCNGG 7 cut(s) 196, 373, 646, 844, 890, 1297, 1639
BstSFI CTRYAG 2 cut(s) 693, 1439
BstSLI GKGCMC 3 cut(s) 365, 1394, 1599
BstUI CGCG 3 cut(s) 528, 926, 1601
BstV1I GCAGC 6 cut(s) 36, 721, 947, 1002, 1390, 1425
BstX2I RGATCY 1 cut(s) 1756
BstXI CCANNNNNNTGG 1 cut(s) 1647
BstYI RGATCY 1 cut(s) 1756
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 2 cut(s) 1394, 1484
BsuRI GGCC 8 cut(s) 60, 283, 343, 373, 767, 843, 1203, 1694
BsuTUI ATCGAT 1 cut(s) 573
BtgI CCRYGG 3 cut(s) 762, 825, 1695
BtsCI GGATG 8 cut(s) 335, 484, 565, 1293, 1471, 1503, 1613, 1675
BtsI GCAGTG 3 cut(s) 1062, 1221, 1599
BtsIMutI CAGTG 9 cut(s) 277, 365, 678, 780, 1062, 1221, 1593, 1599, 1758
BveI ACCTGC 1 cut(s) 1420
Cac8I GCNNGC 5 cut(s) 435, 588, 926, 1228, 1599
CfoI GCGC 3 cut(s) 473, 926, 928
Cfr10I RCCGGY 1 cut(s) 701
Cfr13I GGNCC 6 cut(s) 200, 341, 842, 978, 1010, 1202
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 5 cut(s) 8, 193, 420, 830, 895
CviAII CATG 9 cut(s) 119, 305, 434, 447, 835, 1231, 1264, 1647, 1696
CviQI GTAC 5 cut(s) 8, 193, 420, 830, 895
DdeI CTNAG 4 cut(s) 357, 452, 719, 901
DpnI GATC 4 cut(s) 654, 1420, 1569, 1758
DpnII GATC 4 cut(s) 652, 1418, 1567, 1756
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 2 cut(s) 765, 1692
Eam1104I CTCTTC 2 cut(s) 143, 1142
EarI CTCTTC 2 cut(s) 143, 1142
EciI GGCGGA 1 cut(s) 970
Ecl136II GAGCTC 1 cut(s) 608
Eco130I CCWWGG 3 cut(s) 207, 1198, 1695
Eco24I GRGCYC 1 cut(s) 610
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 3 cut(s) 200, 978, 1010
Eco53kI GAGCTC 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 1259
Eco91I GGTNACC 1 cut(s) 875
EcoICRI GAGCTC 1 cut(s) 608
EcoNI CCTNNNNNAGG 1 cut(s) 492
EcoO109I RGGNCCY 1 cut(s) 842
EcoO65I GGTNACC 1 cut(s) 875
EcoRII CCWGG 6 cut(s) 196, 373, 646, 844, 1297, 1639
EcoT14I CCWWGG 3 cut(s) 207, 1198, 1695
EcoT22I ATGCAT 2 cut(s) 628, 1659
EcoT38I GRGCYC 1 cut(s) 610
ErhI CCWWGG 3 cut(s) 207, 1198, 1695
FaeI CATG 9 cut(s) 122, 308, 437, 450, 838, 1234, 1267, 1650, 1699
FaqI GGGAC 2 cut(s) 1097, 1225
FatI CATG 9 cut(s) 118, 304, 433, 446, 834, 1230, 1263, 1646, 1695
FauI CCCGC 1 cut(s) 1606
Fnu4HI GCNGC 7 cut(s) 25, 735, 906, 936, 991, 1379, 1439
FokI GGATG 8 cut(s) 342, 471, 572, 1280, 1458, 1490, 1620, 1682
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 7 cut(s) 25, 735, 906, 936, 991, 1379, 1439
FspBI CTAG 3 cut(s) 594, 839, 1395
GlaI GCGC 3 cut(s) 472, 925, 927
GluI GCNGC 7 cut(s) 25, 735, 906, 936, 991, 1379, 1439
GsuI CTGGAG 1 cut(s) 1320
HaeIII GGCC 8 cut(s) 60, 283, 343, 373, 767, 843, 1203, 1694
HapII CCGG 2 cut(s) 702, 892
HhaI GCGC 3 cut(s) 473, 926, 928
Hin1II CATG 9 cut(s) 122, 308, 437, 450, 838, 1234, 1267, 1650, 1699
Hin6I GCGC 3 cut(s) 471, 924, 926
HinP1I GCGC 3 cut(s) 471, 924, 926
HincII GTYRAC 1 cut(s) 644
HindII GTYRAC 1 cut(s) 644
HinfI GANTC 6 cut(s) 39, 355, 500, 723, 1158, 1623
HpaII CCGG 2 cut(s) 702, 892
HphI GGTGA 7 cut(s) 104, 236, 560, 811, 887, 1069, 1300
Hpy166II GTNNAC 5 cut(s) 217, 466, 644, 1675, 1711
Hpy188I TCNGA 5 cut(s) 38, 622, 1091, 1184, 1622
Hpy188III TCNNGA 1 cut(s) 968
Hpy8I GTNNAC 5 cut(s) 217, 466, 644, 1675, 1711
Hpy99I CGWCG 2 cut(s) 1130, 1631
HpyAV CCTTC 9 cut(s) 410, 520, 548, 725, 1185, 1255, 1283, 1341, 1508
HpyCH4III ACNGT 9 cut(s) 181, 192, 443, 463, 682, 784, 829, 1010, 1588
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 911, 932, 999, 1362, 1435, 1598
HpyF3I CTNAG 4 cut(s) 357, 452, 719, 901
Hsp92II CATG 9 cut(s) 122, 308, 437, 450, 838, 1234, 1267, 1650, 1699
HspAI GCGC 3 cut(s) 471, 924, 926
Kzo9I GATC 4 cut(s) 652, 1418, 1567, 1756
LmnI GCTCC 3 cut(s) 605, 613, 1741
Lsp1109I GCAGC 6 cut(s) 36, 721, 947, 1002, 1390, 1425
LweI GCATC 4 cut(s) 550, 613, 787, 1461
MaeI CTAG 3 cut(s) 594, 839, 1395
MaeIII GTNAC 7 cut(s) 224, 244, 268, 352, 875, 1456, 1661
MalI GATC 4 cut(s) 654, 1420, 1569, 1758
MboI GATC 4 cut(s) 652, 1418, 1567, 1756
MboII GAAGA 8 cut(s) 160, 281, 533, 748, 1129, 1132, 1348, 1475
MfeI CAATTG 2 cut(s) 661, 1165
MflI RGATCY 1 cut(s) 1756
MhlI GDGCHC 4 cut(s) 365, 610, 1394, 1599
MlsI TGGCCA 2 cut(s) 767, 1694
MluCI AATT 6 cut(s) 347, 402, 661, 1165, 1340, 1525
MluNI TGGCCA 2 cut(s) 767, 1694
MlyI GAGTC 5 cut(s) 33, 349, 494, 732, 1152
MmeI TCCRAC 2 cut(s) 61, 1037
Mox20I TGGCCA 2 cut(s) 767, 1694
Mph1103I ATGCAT 2 cut(s) 628, 1659
MroXI GAANNNNTTC 1 cut(s) 1340
MscI TGGCCA 2 cut(s) 767, 1694
MseI TTAA 4 cut(s) 866, 1107, 1557, 1771
MslI CAYNNNNRTG 2 cut(s) 117, 1668
Msp20I TGGCCA 2 cut(s) 767, 1694
MspA1I CMGCKG 1 cut(s) 1313
MspI CCGG 2 cut(s) 702, 892
MspR9I CCNGG 7 cut(s) 198, 375, 648, 846, 892, 1299, 1641
MunI CAATTG 2 cut(s) 661, 1165
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 6 cut(s) 198, 375, 648, 846, 1299, 1641
MvnI CGCG 3 cut(s) 528, 926, 1601
MwoI GCNNNNNNNGC 7 cut(s) 57, 911, 932, 999, 1362, 1435, 1598
NciI CCSGG 1 cut(s) 892
NcoI CCATGG 1 cut(s) 1695
NdeII GATC 4 cut(s) 652, 1418, 1567, 1756
NlaIII CATG 9 cut(s) 122, 308, 437, 450, 838, 1234, 1267, 1650, 1699
NlaIV GGNNCC 2 cut(s) 1196, 1743
NmuCI GTSAC 6 cut(s) 224, 268, 352, 875, 1456, 1661
NsiI ATGCAT 2 cut(s) 628, 1659
NspI RCATGY 2 cut(s) 437, 1267
NspV TTCGAA 1 cut(s) 1334
PaeI GCATGC 1 cut(s) 437
PasI CCCWGGG 1 cut(s) 845
PauI GCGCGC 1 cut(s) 924
PciI ACATGT 1 cut(s) 1263
PcsI WCGNNNNNNNCGW 1 cut(s) 1461
PctI GAATGC 1 cut(s) 938
PdmI GAANNNNTTC 1 cut(s) 1340
PfeI GAWTC 1 cut(s) 1623
PflFI GACNNNGTC 1 cut(s) 680
PkrI GCNGC 7 cut(s) 26, 736, 907, 937, 992, 1380, 1440
PleI GAGTC 5 cut(s) 33, 349, 494, 731, 1152
PpsI GAGTC 5 cut(s) 33, 349, 494, 731, 1152
PscI ACATGT 1 cut(s) 1263
PsiI TTATAA 1 cut(s) 581
Psp124BI GAGCTC 1 cut(s) 610
Psp6I CCWGG 6 cut(s) 196, 373, 646, 844, 1297, 1639
PspEI GGTNACC 1 cut(s) 875
PspGI CCWGG 6 cut(s) 196, 373, 646, 844, 1297, 1639
PspN4I GGNNCC 2 cut(s) 1196, 1743
PspPI GGNCC 6 cut(s) 200, 341, 842, 978, 1010, 1202
PstI CTGCAG 1 cut(s) 1443
PsuI RGATCY 1 cut(s) 1756
PsyI GACNNNGTC 1 cut(s) 680
PteI GCGCGC 1 cut(s) 924
RsaI GTAC 5 cut(s) 9, 194, 421, 831, 896
RsaNI GTAC 5 cut(s) 8, 193, 420, 830, 895
RseI CAYNNNNRTG 2 cut(s) 117, 1668
SacI GAGCTC 1 cut(s) 610
SaqAI TTAA 4 cut(s) 866, 1107, 1557, 1771
SatI GCNGC 7 cut(s) 25, 735, 906, 936, 991, 1379, 1439
Sau3AI GATC 4 cut(s) 652, 1418, 1567, 1756
Sau96I GGNCC 6 cut(s) 200, 341, 842, 978, 1010, 1202
SchI GAGTC 5 cut(s) 33, 349, 494, 732, 1152
ScrFI CCNGG 7 cut(s) 198, 375, 648, 846, 892, 1299, 1641
SduI GDGCHC 4 cut(s) 365, 610, 1394, 1599
SfaNI GCATC 4 cut(s) 550, 613, 787, 1461
SfcI CTRYAG 2 cut(s) 693, 1439
SfuI TTCGAA 1 cut(s) 1334
SgrAI CRCCGGYG 1 cut(s) 701
SinI GGWCC 3 cut(s) 200, 978, 1010
SmiMI CAYNNNNRTG 2 cut(s) 117, 1668
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
SphI GCATGC 1 cut(s) 437
Sse9I AATT 6 cut(s) 347, 402, 661, 1165, 1340, 1525
SsiI CCGC 5 cut(s) 905, 960, 981, 1311, 1599
SspMI CTAG 3 cut(s) 594, 839, 1395
SstI GAGCTC 1 cut(s) 610
StyD4I CCNGG 7 cut(s) 196, 373, 646, 844, 890, 1297, 1639
StyI CCWWGG 3 cut(s) 207, 1198, 1695
TaaI ACNGT 9 cut(s) 181, 192, 443, 463, 682, 784, 829, 1010, 1588
TaqI TCGA 7 cut(s) 83, 259, 573, 942, 1125, 1334, 1344
TaqII GACCGA 2 cut(s) 1139, 1556
TasI AATT 6 cut(s) 347, 402, 661, 1165, 1340, 1525
TauI GCSGC 1 cut(s) 908
TfiI GAWTC 1 cut(s) 1623
Tru1I TTAA 4 cut(s) 866, 1107, 1557, 1771
Tru9I TTAA 4 cut(s) 866, 1107, 1557, 1771
TscAI CASTG 9 cut(s) 284, 365, 685, 787, 1069, 1228, 1593, 1599, 1758
TseFI GTSAC 6 cut(s) 224, 268, 352, 875, 1456, 1661
TseI GCWGC 6 cut(s) 24, 734, 935, 990, 1378, 1438
Tsp45I GTSAC 6 cut(s) 224, 268, 352, 875, 1456, 1661
TspDTI ATGAA 4 cut(s) 69, 435, 473, 1247
TspGWI ACGGA 2 cut(s) 751, 1503
TspRI CASTG 9 cut(s) 284, 365, 685, 787, 1069, 1228, 1593, 1599, 1758
Tth111I GACNNNGTC 1 cut(s) 680
VpaK11BI GGWCC 3 cut(s) 200, 978, 1010
XagI CCTNNNNNAGG 1 cut(s) 492
XapI RAATTY 3 cut(s) 347, 1340, 1525
XceI RCATGY 2 cut(s) 437, 1267
XcmI CCANNNNNNNNNTGG 1 cut(s) 1702
XmnI GAANNNNTTC 1 cut(s) 1340
XspI CTAG 3 cut(s) 594, 839, 1395
Zsp2I ATGCAT 2 cut(s) 628, 1659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.