MD07G1227900.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001265
Physical Location & Seq
Reverse (-)
30339208 .. 30341644
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1227900.v1.1.491

Sequence Viewer

Length: 1752 bp
ATGGATGCACTCAACGCTACTTTCGGCAACAAAATTTGCTTCTCGTTATGCCTGTTCGGCCTTTGCCTTCTCGTGGCTTCGTCAACAACGTCCTTGGCAGAACCCAAAACTCATAAGCATGAGTTTGTTATTCAAGCAACACCAGTGAAGAGGCTGTGCAAATCCCGAAACTCCATCACAGTGAATGGACAGTTCCCTGGACCAACCTTAGAAGTAAATAATGGCGATACTCTTGTCGTCAAAGTCACCAACAGAGCTCAATACAACGTCACCATCCACTGGCATGGGATTAGGCAGATGAGAACTGGATGGGCAGATGGTCCAGAATTTGTGACTCAGTGCCCAATTAGGTCAGGAGGGAGCTACACTTACCGGTTTACAATTCAAGGGCAAGAAGGTACTCTGTGGTGGCATGCTCACAGCTCATGGCTTAGAGCCACCGTTTACGGAGCACTCATCATTCATCCTAAACAAGGAGACTCCTACCCGTTCACTAAACCGAAACGCGAAACAACCCTTCTTCTCGGTGAATGGTGGAATGCTAACCCTATCAATGTCTTGAGGCAGTCGACTAGGACAGGAGGAGCTCCAAATGTTTCTGATGCATACACCATCAATGGTCAACCTGGTGATCTTTACAATTGCTCAAGCAAAGACACTGTTATAGTTCCTATAGACTCCAGCGAGACCAACCTTCTTAGAGTCATCAATGCTGCCCTCAACCAACCTCTTTTCTTCTCCGTGGCCAACCACAAACTCACCGTTGTTAGTGCTGATGCCTCCTACACCAAACCTTTCACTACCACGGTGCTCATGCTAGGCCCTGGGCAGACCACCGATGTTTTAATCACCGGTGACCAGCCACCAGCCAGGTACTACTTGGCGGCGCGTGCTTATTTCAGCGCTCACAATGCAGCATTCGACAACACCACTACCACAGCCATTCTTGAATACAAATCTGCCCCTTGCAGCCCCAATTGCAAAAAGGGTCCCACAGCCAAACCAATTATGCCACAACTCCCTGCTTTCAATGACACAAACACTGCTTCTGCTTTCACCAAGAGCTTCAGAAGTCCAAGAAAAGTTGAAGTCCCAACTGAAATCGACGAAAACCTCTTCTTCACAATCGGTCTTGGGCTCAACAACTGCCCGAAAAACTTTGGATCCCAAAGGTGCCAAGGCCCGAACGGGACACGTTTCACAGCCAGCATGAACAATGTATCATTTGTGCTTCCAAACAACATCTCAATCCTTCAGGCTTACCAACAGAACATCCCTGGAGTTTTTACTACTGATTTTCCAGCAAACCCTCCGCGGAAATTCGACTACACAGGTAACGTGAGTCGCTCTCTCTGGCAGCCTATGCCGGGCACTAGAGGATACAAGTTGAAGTATGGATCAAGGGTGCAGGTTGTGCTGCAGGACACAAGCATAGTCACACCCGAAAACCATCCTATCCATCTTCACGGATACGATTTTTACATCCTCGCCGAAGGTTTTGGAAATTTCAATGCCAAAACTGATACCAAAAAATTCAACCTTGTTGATCCACCTATGAGGAACACAGTGGCAGTGCCTGCCAATGGATGGGCAGTCATCCGATTTGTTGCTGACAATCCAGGTGCATGGATAATGCATTGTCACTTGGATGTTCACATCAACTGGGGTTTGGCCATGGTGTTCTTGGTGGACAATGGAGTTGGAGCATTGCAGTCAATCAAGCCACCACCGGCGGATCTGCCTCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

584

Amino Acids

64.21

Weight (kDa)

9.06

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 44 - 158 1.8e-44 Multicopper oxidase
Cu-oxidase PF00394 170 - 320 3.6e-41 Multicopper oxidase
Cu-oxidase_2 PF07731 432 - 565 9.2e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1399
AccB1I GGYRCC 1 cut(s) 1173
AccB7I CCANNNNNTGG 2 cut(s) 279, 1587
AccI GTMKAC 1 cut(s) 569
AccII CGCG 3 cut(s) 507, 889, 1315
AciI CCGC 4 cut(s) 884, 1313, 1315, 1733
AclWI GGATC 5 cut(s) 1158, 1171, 1405, 1541, 1743
AcoI YGGCCR 2 cut(s) 744, 1671
AcsI RAATTY 5 cut(s) 33, 326, 1319, 1504, 1532
AcuI CTGAAG 2 cut(s) 1051, 1238
AfaI GTAC 2 cut(s) 400, 875
AfeI AGCGCT 1 cut(s) 904
AfiI CCNNNNNNNGG 6 cut(s) 73, 279, 473, 1367, 1583, 1587
AflIII ACRYGT 1 cut(s) 1193
AgeI ACCGGT 2 cut(s) 372, 851
AgsI TTSAA 8 cut(s) 134, 386, 950, 1030, 1088, 1390, 1510, 1537
AjnI CCWGG 6 cut(s) 196, 625, 823, 869, 1276, 1618
AluBI AGCT 5 cut(s) 257, 363, 423, 587, 1065
AluI AGCT 5 cut(s) 257, 363, 423, 587, 1065
Alw21I GWGCWC 4 cut(s) 259, 454, 589, 813
Alw26I GTCTC 2 cut(s) 471, 680
AlwI GGATC 5 cut(s) 1158, 1171, 1405, 1541, 1743
AlwNI CAGNNNCTG 1 cut(s) 1577
Aor51HI AGCGCT 1 cut(s) 904
AoxI GGCC 5 cut(s) 58, 744, 820, 1180, 1671
ApeKI GCWGC 5 cut(s) 713, 914, 969, 1357, 1417
ApoI RAATTY 5 cut(s) 33, 326, 1319, 1504, 1532
AsiGI ACCGGT 2 cut(s) 372, 851
AspLEI GCGC 2 cut(s) 889, 905
AspS9I GGNCC 5 cut(s) 200, 320, 821, 989, 1181
AsuC2I CCSGG 1 cut(s) 1368
AsuHPI GGTGA 8 cut(s) 238, 262, 539, 641, 751, 841, 866, 1048
AvaII GGWCC 3 cut(s) 200, 320, 989
BaeGI GKGCMC 2 cut(s) 344, 1373
BaeI ACNNNNGTAYC 2 cut(s) 219, 252
BalI TGGCCA 2 cut(s) 746, 1673
BamHI GGATCC 1 cut(s) 1163
BanI GGYRCC 1 cut(s) 1173
BanII GRGCYC 3 cut(s) 259, 589, 1140
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 4 cut(s) 259, 454, 589, 813
BbvI GCAGC 5 cut(s) 700, 926, 981, 1369, 1404
BccI CCATC 8 cut(s) 182, 281, 303, 311, 620, 1458, 1467, 1581
BciT130I CCWGG 6 cut(s) 198, 627, 825, 871, 1278, 1620
BciVI GTATCC 2 cut(s) 1373, 1463
BcnI CCSGG 1 cut(s) 1368
BcoDI GTCTC 2 cut(s) 471, 680
BfaI CTAG 3 cut(s) 573, 818, 1374
BfmI CTRYAG 2 cut(s) 672, 1418
BfoI RGCGCY 1 cut(s) 906
BfuAI ACCTGC 1 cut(s) 1399
BfuI GTATCC 2 cut(s) 1373, 1463
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 6 cut(s) 714, 885, 915, 970, 1358, 1418
BlsI GCNGC 6 cut(s) 715, 886, 916, 971, 1359, 1419
Bme1390I CCNGG 7 cut(s) 198, 627, 825, 871, 1278, 1368, 1620
Bme18I GGWCC 3 cut(s) 200, 320, 989
BmgT120I GGNCC 5 cut(s) 200, 320, 821, 989, 1181
BmiI GGNNCC 4 cut(s) 990, 991, 1165, 1175
BmrFI CCNGG 7 cut(s) 198, 627, 825, 871, 1278, 1368, 1620
BmrI ACTGGG 1 cut(s) 1672
BmsI GCATC 2 cut(s) 592, 766
BmuI ACTGGG 1 cut(s) 1672
BplI GAGNNNNNCTC 2 cut(s) 1333, 1365
BpmI CTGGAG 2 cut(s) 664, 1299
BpuEI CTTGAG 2 cut(s) 580, 631
BpuMI CCSGG 1 cut(s) 1368
BsaI GGTCTC 1 cut(s) 680
BsaWI WCCGGW 2 cut(s) 372, 851
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 279, 473, 1367, 1583, 1587
Bse118I RCCGGY 3 cut(s) 372, 851, 1729
Bse1I ACTGG 4 cut(s) 143, 284, 310, 1667
Bse3DI GCAATG 1 cut(s) 1706
BseBI CCWGG 6 cut(s) 198, 627, 825, 871, 1278, 1620
BseLI CCNNNNNNNGG 6 cut(s) 73, 279, 473, 1367, 1583, 1587
BseMI GCAATG 1 cut(s) 1706
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 4 cut(s) 143, 284, 310, 1667
BseRI GAGGAG 1 cut(s) 597
BseSI GKGCMC 2 cut(s) 344, 1373
BseXI GCAGC 5 cut(s) 700, 926, 981, 1369, 1404
BsgI GTGCAG 1 cut(s) 1427
Bsh1236I CGCG 3 cut(s) 507, 889, 1315
BshFI GGCC 5 cut(s) 60, 746, 822, 1182, 1673
BshNI GGYRCC 1 cut(s) 1173
BshTI ACCGGT 2 cut(s) 372, 851
BsiHKAI GWGCWC 4 cut(s) 259, 454, 589, 813
BsiSI CCGG 4 cut(s) 373, 852, 1367, 1730
BslFI GGGAC 3 cut(s) 975, 1076, 1204
BslI CCNNNNNNNGG 6 cut(s) 73, 279, 473, 1367, 1583, 1587
BsmAI GTCTC 2 cut(s) 471, 680
BsmFI GGGAC 3 cut(s) 975, 1076, 1204
BsmI GAATGC 2 cut(s) 544, 917
BsnI GGCC 5 cut(s) 60, 746, 822, 1182, 1673
Bso31I GGTCTC 1 cut(s) 680
Bsp1286I GDGCHC 7 cut(s) 259, 344, 454, 589, 813, 1140, 1373
Bsp143I GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
Bsp19I CCATGG 1 cut(s) 1674
BspACI CCGC 4 cut(s) 884, 1313, 1315, 1733
BspANI GGCC 5 cut(s) 60, 746, 822, 1182, 1673
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 3 cut(s) 507, 889, 1315
BspLI GGNNCC 4 cut(s) 990, 991, 1165, 1175
BspMAI CTGCAG 1 cut(s) 1422
BspMI ACCTGC 1 cut(s) 1399
BspPI GGATC 5 cut(s) 1158, 1171, 1405, 1541, 1743
BspT107I GGYRCC 1 cut(s) 1173
BspTNI GGTCTC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 1706
BsrFI RCCGGY 3 cut(s) 372, 851, 1729
BsrI ACTGG 4 cut(s) 143, 284, 310, 1667
BssAI RCCGGY 3 cut(s) 372, 851, 1729
BssMI GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 3 cut(s) 93, 1177, 1674
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 6 cut(s) 198, 627, 825, 871, 1278, 1620
Bst4CI ACNGT 7 cut(s) 181, 192, 442, 661, 763, 808, 1567
Bst6I CTCTTC 2 cut(s) 143, 1121
BstAPI GCANNNNNTGC 3 cut(s) 1363, 1414, 1577
BstC8I GCNNGC 4 cut(s) 414, 891, 1207, 1578
BstDEI CTNAG 4 cut(s) 208, 336, 431, 698
BstDSI CCRYGG 4 cut(s) 741, 804, 1313, 1674
BstEII GGTNACC 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 471
BstFNI CGCG 3 cut(s) 507, 889, 1315
BstH2I RGCGCY 1 cut(s) 906
BstHHI GCGC 2 cut(s) 889, 905
BstKTI GATC 5 cut(s) 634, 1166, 1400, 1549, 1738
BstMAI GTCTC 2 cut(s) 471, 680
BstMBI GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
BstMWI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1363, 1414, 1577
BstNI CCWGG 6 cut(s) 198, 627, 825, 871, 1278, 1620
BstNSI RCATGY 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 7 cut(s) 196, 625, 823, 869, 1276, 1366, 1618
BstSFI CTRYAG 2 cut(s) 672, 1418
BstSLI GKGCMC 2 cut(s) 344, 1373
BstUI CGCG 3 cut(s) 507, 889, 1315
BstV1I GCAGC 5 cut(s) 700, 926, 981, 1369, 1404
BstX2I RGATCY 2 cut(s) 1163, 1735
BstXI CCANNNNNNTGG 2 cut(s) 284, 1626
BstYI RGATCY 2 cut(s) 1163, 1735
BsuI GTATCC 2 cut(s) 1373, 1463
BsuRI GGCC 5 cut(s) 60, 746, 822, 1182, 1673
BtgI CCRYGG 4 cut(s) 741, 804, 1313, 1674
BtsI GCAGTG 2 cut(s) 1041, 1578
BtsIMutI CAGTG 8 cut(s) 150, 186, 277, 344, 657, 1041, 1572, 1578
BveI ACCTGC 1 cut(s) 1399
Cac8I GCNNGC 4 cut(s) 414, 891, 1207, 1578
CaiI CAGNNNCTG 1 cut(s) 1577
CfoI GCGC 2 cut(s) 889, 905
Cfr10I RCCGGY 3 cut(s) 372, 851, 1729
Cfr13I GGNCC 5 cut(s) 200, 320, 821, 989, 1181
Cfr42I CCGCGG 1 cut(s) 1316
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 2 cut(s) 399, 874
CspAI ACCGGT 2 cut(s) 372, 851
CviAII CATG 8 cut(s) 119, 284, 413, 426, 814, 1210, 1626, 1675
CviQI GTAC 2 cut(s) 399, 874
DdeI CTNAG 4 cut(s) 208, 336, 431, 698
DpnI GATC 5 cut(s) 633, 1165, 1399, 1548, 1737
DpnII GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
EaeI YGGCCR 2 cut(s) 744, 1671
Eam1104I CTCTTC 2 cut(s) 143, 1121
EarI CTCTTC 2 cut(s) 143, 1121
EciI GGCGGA 1 cut(s) 1748
Ecl136II GAGCTC 2 cut(s) 257, 587
Eco130I CCWWGG 3 cut(s) 93, 1177, 1674
Eco24I GRGCYC 3 cut(s) 259, 589, 1140
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 3 cut(s) 200, 320, 989
Eco47III AGCGCT 1 cut(s) 904
Eco53kI GAGCTC 2 cut(s) 257, 587
Eco57I CTGAAG 2 cut(s) 1051, 1238
Eco91I GGTNACC 1 cut(s) 854
EcoICRI GAGCTC 2 cut(s) 257, 587
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 2 cut(s) 821, 989
EcoO65I GGTNACC 1 cut(s) 854
EcoRII CCWGG 6 cut(s) 196, 625, 823, 869, 1276, 1618
EcoT14I CCWWGG 3 cut(s) 93, 1177, 1674
EcoT22I ATGCAT 2 cut(s) 607, 1638
EcoT38I GRGCYC 3 cut(s) 259, 589, 1140
ErhI CCWWGG 3 cut(s) 93, 1177, 1674
FaeI CATG 8 cut(s) 122, 287, 416, 429, 817, 1213, 1629, 1678
FaqI GGGAC 3 cut(s) 975, 1076, 1204
FatI CATG 8 cut(s) 118, 283, 412, 425, 813, 1209, 1625, 1674
FblI GTMKAC 1 cut(s) 569
Fnu4HI GCNGC 6 cut(s) 714, 885, 915, 970, 1358, 1418
FriOI GRGCYC 3 cut(s) 259, 589, 1140
Fsp4HI GCNGC 6 cut(s) 714, 885, 915, 970, 1358, 1418
FspBI CTAG 3 cut(s) 573, 818, 1374
GlaI GCGC 2 cut(s) 888, 904
GluI GCNGC 6 cut(s) 714, 885, 915, 970, 1358, 1418
GsuI CTGGAG 2 cut(s) 664, 1299
HaeII RGCGCY 1 cut(s) 906
HaeIII GGCC 5 cut(s) 60, 746, 822, 1182, 1673
HapII CCGG 4 cut(s) 373, 852, 1367, 1730
HhaI GCGC 2 cut(s) 889, 905
Hin1II CATG 8 cut(s) 122, 287, 416, 429, 817, 1213, 1629, 1678
Hin6I GCGC 2 cut(s) 887, 903
HinP1I GCGC 2 cut(s) 887, 903
HincII GTYRAC 3 cut(s) 84, 570, 623
HindII GTYRAC 3 cut(s) 84, 570, 623
HinfI GANTC 5 cut(s) 334, 479, 677, 702, 1342
HpaII CCGG 4 cut(s) 373, 852, 1367, 1730
HphI GGTGA 8 cut(s) 238, 262, 539, 641, 751, 841, 866, 1048
Hpy166II GTNNAC 8 cut(s) 84, 378, 445, 492, 570, 623, 1654, 1690
Hpy188I TCNGA 3 cut(s) 601, 1070, 1601
Hpy188III TCNNGA 5 cut(s) 165, 323, 354, 559, 947
Hpy8I GTNNAC 8 cut(s) 84, 378, 445, 492, 570, 623, 1654, 1690
Hpy99I CGWCG 1 cut(s) 1109
HpyAV CCTTC 6 cut(s) 77, 389, 527, 704, 1262, 1487
HpyCH4III ACNGT 7 cut(s) 181, 192, 442, 661, 763, 808, 1567
HpyCH4IV ACGT 4 cut(s) 89, 267, 1195, 1338
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1363, 1414, 1577
HpyF3I CTNAG 4 cut(s) 208, 336, 431, 698
HpySE526I ACGT 4 cut(s) 89, 267, 1195, 1338
Hsp92II CATG 8 cut(s) 122, 287, 416, 429, 817, 1213, 1629, 1678
HspAI GCGC 2 cut(s) 887, 903
KflI GGGWCCC 1 cut(s) 989
KspI CCGCGG 1 cut(s) 1316
Kzo9I GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
LmnI GCTCC 5 cut(s) 360, 449, 584, 592, 1703
Lsp1109I GCAGC 5 cut(s) 700, 926, 981, 1369, 1404
LweI GCATC 2 cut(s) 592, 766
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 3 cut(s) 573, 818, 1374
MaeII ACGT 4 cut(s) 89, 267, 1195, 1338
MaeIII GTNAC 7 cut(s) 244, 268, 331, 854, 1334, 1435, 1640
MalI GATC 5 cut(s) 633, 1165, 1399, 1548, 1737
MboI GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
MboII GAAGA 6 cut(s) 160, 512, 727, 1108, 1111, 1454
MfeI CAATTG 2 cut(s) 640, 976
MflI RGATCY 2 cut(s) 1163, 1735
MhlI GDGCHC 7 cut(s) 259, 344, 454, 589, 813, 1140, 1373
MlsI TGGCCA 2 cut(s) 746, 1673
MluNI TGGCCA 2 cut(s) 746, 1673
MlyI GAGTC 5 cut(s) 328, 473, 671, 711, 1351
MmeI TCCRAC 1 cut(s) 1681
Mox20I TGGCCA 2 cut(s) 746, 1673
Mph1103I ATGCAT 2 cut(s) 607, 1638
MscI TGGCCA 2 cut(s) 746, 1673
MseI TTAA 2 cut(s) 845, 1750
MslI CAYNNNNRTG 4 cut(s) 117, 179, 282, 1647
Msp20I TGGCCA 2 cut(s) 746, 1673
MspA1I CMGCKG 1 cut(s) 1315
MspI CCGG 4 cut(s) 373, 852, 1367, 1730
MspR9I CCNGG 7 cut(s) 198, 627, 825, 871, 1278, 1368, 1620
MunI CAATTG 2 cut(s) 640, 976
Mva1269I GAATGC 2 cut(s) 544, 917
MvaI CCWGG 6 cut(s) 198, 627, 825, 871, 1278, 1620
MvnI CGCG 3 cut(s) 507, 889, 1315
MwoI GCNNNNNNNGC 8 cut(s) 14, 57, 890, 911, 978, 1363, 1414, 1577
NciI CCSGG 1 cut(s) 1368
NcoI CCATGG 1 cut(s) 1674
NdeII GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
NlaIII CATG 8 cut(s) 122, 287, 416, 429, 817, 1213, 1629, 1678
NlaIV GGNNCC 4 cut(s) 990, 991, 1165, 1175
NmuCI GTSAC 6 cut(s) 244, 268, 331, 854, 1435, 1640
NsiI ATGCAT 2 cut(s) 607, 1638
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PasI CCCWGGG 1 cut(s) 824
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PctI GAATGC 2 cut(s) 544, 917
PflMI CCANNNNNTGG 2 cut(s) 279, 1587
PinAI ACCGGT 2 cut(s) 372, 851
PkrI GCNGC 6 cut(s) 715, 886, 916, 971, 1359, 1419
PleI GAGTC 5 cut(s) 328, 473, 671, 710, 1350
PpsI GAGTC 5 cut(s) 328, 473, 671, 710, 1350
PpuMI RGGWCCY 1 cut(s) 989
Psp124BI GAGCTC 2 cut(s) 259, 589
Psp5II RGGWCCY 1 cut(s) 989
Psp6I CCWGG 6 cut(s) 196, 625, 823, 869, 1276, 1618
PspEI GGTNACC 1 cut(s) 854
PspGI CCWGG 6 cut(s) 196, 625, 823, 869, 1276, 1618
PspN4I GGNNCC 4 cut(s) 990, 991, 1165, 1175
PspPI GGNCC 5 cut(s) 200, 320, 821, 989, 1181
PspPPI RGGWCCY 1 cut(s) 989
PstI CTGCAG 1 cut(s) 1422
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 2 cut(s) 1163, 1735
RsaI GTAC 2 cut(s) 400, 875
RsaNI GTAC 2 cut(s) 399, 874
RseI CAYNNNNRTG 4 cut(s) 117, 179, 282, 1647
SacI GAGCTC 2 cut(s) 259, 589
SacII CCGCGG 1 cut(s) 1316
SalI GTCGAC 1 cut(s) 568
SaqAI TTAA 2 cut(s) 845, 1750
SatI GCNGC 6 cut(s) 714, 885, 915, 970, 1358, 1418
Sau3AI GATC 5 cut(s) 631, 1163, 1397, 1546, 1735
Sau96I GGNCC 5 cut(s) 200, 320, 821, 989, 1181
SchI GAGTC 5 cut(s) 328, 473, 671, 711, 1351
ScrFI CCNGG 7 cut(s) 198, 627, 825, 871, 1278, 1368, 1620
SduI GDGCHC 7 cut(s) 259, 344, 454, 589, 813, 1140, 1373
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 2 cut(s) 592, 766
SfcI CTRYAG 2 cut(s) 672, 1418
Sfr303I CCGCGG 1 cut(s) 1316
SgrAI CRCCGGYG 2 cut(s) 851, 1729
SgrBI CCGCGG 1 cut(s) 1316
SinI GGWCC 3 cut(s) 200, 320, 989
SmiMI CAYNNNNRTG 4 cut(s) 117, 179, 282, 1647
SmlI CTYRAG 2 cut(s) 559, 646
SmoI CTYRAG 2 cut(s) 559, 646
SphI GCATGC 1 cut(s) 416
SsiI CCGC 4 cut(s) 884, 1313, 1315, 1733
SspMI CTAG 3 cut(s) 573, 818, 1374
SstI GAGCTC 2 cut(s) 259, 589
StyD4I CCNGG 7 cut(s) 196, 625, 823, 869, 1276, 1366, 1618
StyI CCWWGG 3 cut(s) 93, 1177, 1674
TaaI ACNGT 7 cut(s) 181, 192, 442, 661, 763, 808, 1567
TaiI ACGT 4 cut(s) 92, 270, 1198, 1341
TaqI TCGA 4 cut(s) 569, 921, 1104, 1323
TaqII GACCGA 1 cut(s) 1118
TauI GCSGC 1 cut(s) 887
Tru1I TTAA 2 cut(s) 845, 1750
Tru9I TTAA 2 cut(s) 845, 1750
TscAI CASTG 8 cut(s) 150, 186, 284, 344, 664, 1048, 1572, 1578
TseFI GTSAC 6 cut(s) 244, 268, 331, 854, 1435, 1640
TseI GCWGC 5 cut(s) 713, 914, 969, 1357, 1417
Tsp45I GTSAC 6 cut(s) 244, 268, 331, 854, 1435, 1640
TspDTI ATGAA 2 cut(s) 452, 1226
TspGWI ACGGA 3 cut(s) 462, 730, 1482
TspRI CASTG 8 cut(s) 150, 186, 284, 344, 664, 1048, 1572, 1578
Van91I CCANNNNNTGG 2 cut(s) 279, 1587
VpaK11BI GGWCC 3 cut(s) 200, 320, 989
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 5 cut(s) 33, 326, 1319, 1504, 1532
XceI RCATGY 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 2 cut(s) 877, 1681
XmiI GTMKAC 1 cut(s) 569
XspI CTAG 3 cut(s) 573, 818, 1374
Zsp2I ATGCAT 2 cut(s) 607, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.