Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001681

Physical Location & Seq

Genomic Coordinates Forward (+)

814503 .. 816299

Gene Structure 1797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1005700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGGCAAGGGAGGCCAATCCTGCAATGGCATGGTTAGACAAGCCAAACCTGTTGAGCAGGAAAACATGGCTGCTTGGCTTGTTGATGTCAACACCATCAAGATCCTACCTTTCAAGCTCCCCAGTATCGGACCCAATGATGTTCGAATTCGGATCAAGGCTGTCGGCATTTGTGGAAGTGATGTTCACTACCTCAAGACCATGAAATGCGCGGATTTTGAGGTTAAAGAGCCAATGGTAATCGGACATGAGTGTGCCGGGATCGTAGACAAAGTTGGGAGCGAGGTGAAGCATTTGGTGCCTGGTGACCGCGTGGCGGTAGAGCCTGGCATCAGCTGCGCACGGTGCCAGCAGTGCAAGGGAGGCCGCTATAATCTTTGCCCTGATATGAAGTTTTTCGCCACCCCACCGGTTCATGGTTCCTTGGCTAATCAGATTGTGCACCCTGCGGATCTGTGCTTTAAATTGCCGGAAAACGTGAGTTTGGAGGAAGGGGCAATGTGTGAGCCCTTGAGTGTTGGGGTTCACGCTTGTCGGCGAGCCAATGTTGGTCCCGAGACAACTGTCCTGATCATCGGAGCAGGGCCTATTGGTCTCGTTTCAGTTTTAGCCGCTCGTGCTTTCGGGGCACCAAGAATTGTGATTGTGGATATGGATGACAAGCGTCTAGCAATGGCAAAGTCTCTCGGTGCTGATGAAGCCGTGAAAGTTTCGACAAAAATGGAGGATTTAGATGATGAAGTTGCCGAAATTAAAAAAGCCATGATCTCCGAAGTGGATGTGACCTTCGATTGTGTGGGTTTCAACAAAACCATGTCGACCGGCCTCAATGCTACTCGTCCCGGCGGAAAAGTCTGCCTTGTAGGAATGGGACACGGTGTGATGACGGTGCCTCTGACTCCCGCTGCTGCTAGGGAGGTTGACGTTGTTGGAGTTTTCCGATACCAGAACACATGGCCGCTTTGCCTCGAGTTTTTGAGAAGTGGGAAGATTGACGTGAAGCCGCTTATCACACACCGTTTTGGATTTACTGAGAAGGAGGTGGAAGAAGCGTTTGCAACAAGTGCTCGTGGGGGTAATGCCATCAAGGTGATGTTCAAATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003939 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0008150 GO:0008152 GO:0009506 GO:0016491 GO:0016614 GO:0016616 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00040 ko00051 ko01100 map00040 map00051 map01100

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

369

Amino Acids

39.52

Weight (kDa)

7.49

Isoelectric Point (pI)

25.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	44 - 158	8.2e-29	Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C	PF16912	165 - 293	5.3e-09	Glucose dehydrogenase C-terminus
ADH_zinc_N	PF00107	196 - 326	7.8e-29	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	229 - 359	1.7e-07	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51970 AT5G51970
fragaria_vesca	FvH4_7g28870 FvH4_7g28870
malus_domestica	MD00G1005600.v1.1 MD00G1005700.v1.1 MD01G1110100.v1.1 MD01G1194600.v1.1 MD01G1194700.v1.1 MD01G1195200.v1.1 MD01G1195300.v1.1 MD01G1195400.v1.1 MD01G1195500.v1.1 MD07G1261900.v1.1 MD07G1262100.v1.1 MD07G1262300.v1.1
prunus_persica	Prupe.2G288800_v2.0.a1 Prupe.2G288800_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.8G142900_v2.0.a1 Prupe.8G143000_v2.0.a1
pyrus_communis	pycom01g13720 pycom01g20540 pycom01g20550 pycom01g20560 pycom01g20580 pycom01g20600 pycom01g20610 pycom07g24070 pycom07g24080 pycom07g24090 pycom07g24100 pycom07g24130
rosa_chinensis	RchiOBHm_Chr1g0376551
rosa_laevigata	RLG00000026602
rosa_multiflora	Rmu_sc0001308.1_g000011 Rmu_sc0005594.1_g000014
rosa_roxburghii	Rroxscaffold_4G00282010 Rroxscaffold_4G00282130
rosa_rugosa	Rorug01G0396500
rosa_samantha	Rh1AG407600 Rh1AG412800 Rh1BG372300 Rh1CG385100 Rh1CG386100 Rh1DG402100 Rh1DG403200
rosa_wichuraiana	Rw1G036180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	340
AccB1I	GGYRCC	4 cut(s)	298, 345, 628, 889
AccBSI	CCGCTC	1 cut(s)	614
AccI	GTMKAC	2 cut(s)	267, 818
AccII	CGCG	2 cut(s)	212, 312
AclWI	GGATC	4 cut(s)	97, 161, 269, 459
AcoI	YGGCCR	1 cut(s)	956
AcsI	RAATTY	1 cut(s)	147
AdeI	CACNNNGTG	1 cut(s)	880
AfiI	CCNNNNNNNGG	3 cut(s)	128, 316, 416
AgeI	ACCGGT	1 cut(s)	409
AgsI	TTSAA	3 cut(s)	115, 805, 1099
AjiI	CACGTC	1 cut(s)	997
AjnI	CCWGG	2 cut(s)	301, 325
AloI	GAACNNNNNNTCC	2 cut(s)	168, 200
AluBI	AGCT	2 cut(s)	118, 336
AluI	AGCT	2 cut(s)	118, 336
Alw21I	GWGCWC	2 cut(s)	444, 1069
Alw26I	GTCTC	3 cut(s)	551, 599, 687
Alw44I	GTGCAC	1 cut(s)	440
AlwI	GGATC	4 cut(s)	97, 161, 269, 459
Ama87I	CYCGRG	2 cut(s)	554, 968
AoxI	GGCC	5 cut(s)	13, 364, 584, 823, 956
ApaLI	GTGCAC	1 cut(s)	440
ApeKI	GCWGC	4 cut(s)	71, 336, 905, 908
ApoI	RAATTY	1 cut(s)	147
AsiGI	ACCGGT	1 cut(s)	409
Asp700I	GAANNNNTTC	1 cut(s)	395
AspLEI	GCGC	2 cut(s)	212, 341
AspS9I	GGNCC	3 cut(s)	131, 551, 584
AsuC2I	CCSGG	2 cut(s)	259, 843
AsuHPI	GGTGA	3 cut(s)	298, 317, 1102
AsuII	TTCGAA	1 cut(s)	145
AvaI	CYCGRG	2 cut(s)	554, 968
AvaII	GGWCC	2 cut(s)	131, 551
BaeGI	GKGCMC	2 cut(s)	444, 631
BanI	GGYRCC	4 cut(s)	298, 345, 628, 889
BanII	GRGCYC	1 cut(s)	510
BauI	CACGAG	2 cut(s)	615, 1068
Bbv12I	GWGCWC	2 cut(s)	444, 1069
BbvI	GCAGC	4 cut(s)	58, 323, 892, 895
BccI	CCATC	2 cut(s)	104, 1091
BceAI	ACGGC	1 cut(s)	686
BciT130I	CCWGG	2 cut(s)	303, 327
BclI	TGATCA	1 cut(s)	570
BcnI	CCSGG	2 cut(s)	259, 843
BcoDI	GTCTC	3 cut(s)	551, 599, 687
BfaI	CTAG	2 cut(s)	668, 912
BisI	GCNGC	8 cut(s)	72, 337, 367, 612, 906, 909, 959, 1004
BlsI	GCNGC	8 cut(s)	73, 338, 368, 613, 907, 910, 960, 1005
Bme1390I	CCNGG	4 cut(s)	259, 303, 327, 843
Bme18I	GGWCC	2 cut(s)	131, 551
BmeT110I	CYCGRG	2 cut(s)	554, 968
BmgBI	CACGTC	1 cut(s)	997
BmgT120I	GGNCC	3 cut(s)	131, 551, 584
BmiI	GGNNCC	7 cut(s)	133, 300, 347, 421, 553, 630, 891
BmrFI	CCNGG	4 cut(s)	259, 303, 327, 843
BmrI	ACTGGG	1 cut(s)	117
BmsI	GCATC	1 cut(s)	339
BmuI	ACTGGG	1 cut(s)	117
BoxI	GACNNNNGTC	2 cut(s)	563, 663
Bpu14I	TTCGAA	1 cut(s)	145
BpuEI	CTTGAG	2 cut(s)	179, 532
BpuMI	CCSGG	2 cut(s)	259, 843
BsaI	GGTCTC	1 cut(s)	599
BsaJI	CCNNGG	1 cut(s)	423
BsaWI	WCCGGW	1 cut(s)	409
Bsc4I	CCNNNNNNNGG	3 cut(s)	128, 316, 416
Bse118I	RCCGGY	2 cut(s)	409, 821
Bse1I	ACTGG	1 cut(s)	123
Bse3DI	GCAATG	3 cut(s)	31, 504, 678
BseBI	CCWGG	2 cut(s)	303, 327
BseDI	CCNNGG	1 cut(s)	423
BseGI	GGATG	2 cut(s)	661, 784
BseLI	CCNNNNNNNGG	3 cut(s)	128, 316, 416
BseMI	GCAATG	3 cut(s)	31, 504, 678
BseMII	CTCAG	1 cut(s)	1023
BseNI	ACTGG	1 cut(s)	123
BseSI	GKGCMC	2 cut(s)	444, 631
BseXI	GCAGC	4 cut(s)	58, 323, 892, 895
Bsh1236I	CGCG	2 cut(s)	212, 312
Bsh1285I	CGRYCG	1 cut(s)	822
BshFI	GGCC	5 cut(s)	15, 366, 586, 825, 958
BshNI	GGYRCC	4 cut(s)	298, 345, 628, 889
BshTI	ACCGGT	1 cut(s)	409
BsiEI	CGRYCG	1 cut(s)	822
BsiHKAI	GWGCWC	2 cut(s)	444, 1069
BsiHKCI	CYCGRG	2 cut(s)	554, 968
BsiSI	CCGG	5 cut(s)	258, 410, 470, 822, 843
BslFI	GGGAC	3 cut(s)	537, 825, 885
BslI	CCNNNNNNNGG	3 cut(s)	128, 316, 416
BsmAI	GTCTC	3 cut(s)	551, 599, 687
BsmFI	GGGAC	3 cut(s)	537, 825, 885
BsnI	GGCC	5 cut(s)	15, 366, 586, 825, 958
Bso31I	GGTCTC	1 cut(s)	599
BsoBI	CYCGRG	2 cut(s)	554, 968
Bsp119I	TTCGAA	1 cut(s)	145
Bsp1286I	GDGCHC	4 cut(s)	444, 510, 631, 1069
Bsp143I	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
BspANI	GGCC	5 cut(s)	15, 366, 586, 825, 958
BspCNI	CTCAG	1 cut(s)	1024
BspFNI	CGCG	2 cut(s)	212, 312
BspLI	GGNNCC	7 cut(s)	133, 300, 347, 421, 553, 630, 891
BspPI	GGATC	4 cut(s)	97, 161, 269, 459
BspT104I	TTCGAA	1 cut(s)	145
BspT107I	GGYRCC	4 cut(s)	298, 345, 628, 889
BspTNI	GGTCTC	1 cut(s)	599
BsrBI	CCGCTC	1 cut(s)	614
BsrDI	GCAATG	3 cut(s)	31, 504, 678
BsrFI	RCCGGY	2 cut(s)	409, 821
BsrI	ACTGG	1 cut(s)	123
BssAI	RCCGGY	2 cut(s)	409, 821
BssECI	CCNNGG	1 cut(s)	423
BssMI	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
BssSI	CACGAG	2 cut(s)	615, 1068
BssT1I	CCWWGG	1 cut(s)	423
Bst2BI	CACGAG	2 cut(s)	615, 1068
Bst2UI	CCWGG	2 cut(s)	303, 327
Bst4CI	ACNGT	5 cut(s)	345, 565, 878, 889, 1019
BstAPI	GCANNNNNTGC	3 cut(s)	298, 336, 1064
BstBI	TTCGAA	1 cut(s)	145
BstC8I	GCNNGC	2 cut(s)	350, 540
BstDEI	CTNAG	1 cut(s)	1032
BstEII	GGTNACC	1 cut(s)	305
BstF5I	GGATG	2 cut(s)	661, 784
BstFNI	CGCG	2 cut(s)	212, 312
BstHHI	GCGC	2 cut(s)	212, 341
BstKTI	GATC	6 cut(s)	105, 156, 264, 454, 573, 768
BstMAI	GTCTC	3 cut(s)	551, 599, 687
BstMBI	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
BstMCI	CGRYCG	1 cut(s)	822
BstNI	CCWGG	2 cut(s)	303, 327
BstPAI	GACNNNNGTC	2 cut(s)	563, 663
BstPI	GGTNACC	1 cut(s)	305
BstSCI	CCNGG	4 cut(s)	257, 301, 325, 841
BstSLI	GKGCMC	2 cut(s)	444, 631
BstUI	CGCG	2 cut(s)	212, 312
BstV1I	GCAGC	4 cut(s)	58, 323, 892, 895
BstX2I	RGATCY	2 cut(s)	102, 451
BstYI	RGATCY	2 cut(s)	102, 451
BsuRI	GGCC	5 cut(s)	15, 366, 586, 825, 958
BtrI	CACGTC	1 cut(s)	997
BtsCI	GGATG	2 cut(s)	661, 784
BtsI	GCAGTG	1 cut(s)	359
BtsIMutI	CAGTG	1 cut(s)	359
Cac8I	GCNNGC	2 cut(s)	350, 540
CfoI	GCGC	2 cut(s)	212, 341
Cfr10I	RCCGGY	2 cut(s)	409, 821
Cfr13I	GGNCC	3 cut(s)	131, 551, 584
CseI	GACGC	1 cut(s)	653
CspAI	ACCGGT	1 cut(s)	409
CviAII	CATG	8 cut(s)	31, 67, 202, 248, 416, 763, 814, 954
DdeI	CTNAG	1 cut(s)	1032
DpnI	GATC	6 cut(s)	104, 155, 263, 453, 572, 767
DpnII	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
DraI	TTTAAA	1 cut(s)	463
DraIII	CACNNNGTG	1 cut(s)	880
EaeI	YGGCCR	1 cut(s)	956
EciI	GGCGGA	1 cut(s)	861
Eco130I	CCWWGG	1 cut(s)	423
Eco24I	GRGCYC	1 cut(s)	510
Eco31I	GGTCTC	1 cut(s)	599
Eco47I	GGWCC	2 cut(s)	131, 551
Eco88I	CYCGRG	2 cut(s)	554, 968
Eco91I	GGTNACC	1 cut(s)	305
EcoO109I	RGGNCCY	1 cut(s)	584
EcoO65I	GGTNACC	1 cut(s)	305
EcoRI	GAATTC	1 cut(s)	147
EcoRII	CCWGG	2 cut(s)	301, 325
EcoT14I	CCWWGG	1 cut(s)	423
EcoT38I	GRGCYC	1 cut(s)	510
ErhI	CCWWGG	1 cut(s)	423
FaeI	CATG	8 cut(s)	34, 70, 205, 251, 419, 766, 817, 957
FalI	AAGNNNNNCTT	2 cut(s)	843, 875
FaqI	GGGAC	3 cut(s)	537, 825, 885
FatI	CATG	8 cut(s)	30, 66, 201, 247, 415, 762, 813, 953
FauI	CCCGC	1 cut(s)	910
FbaI	TGATCA	1 cut(s)	570
FblI	GTMKAC	2 cut(s)	267, 818
Fnu4HI	GCNGC	8 cut(s)	72, 337, 367, 612, 906, 909, 959, 1004
FokI	GGATG	2 cut(s)	668, 791
FriOI	GRGCYC	1 cut(s)	510
Fsp4HI	GCNGC	8 cut(s)	72, 337, 367, 612, 906, 909, 959, 1004
FspBI	CTAG	2 cut(s)	668, 912
FspI	TGCGCA	1 cut(s)	340
GlaI	GCGC	2 cut(s)	211, 340
GluI	GCNGC	8 cut(s)	72, 337, 367, 612, 906, 909, 959, 1004
HaeIII	GGCC	5 cut(s)	15, 366, 586, 825, 958
HapII	CCGG	5 cut(s)	258, 410, 470, 822, 843
HgaI	GACGC	1 cut(s)	653
HhaI	GCGC	2 cut(s)	212, 341
Hin1II	CATG	8 cut(s)	34, 70, 205, 251, 419, 766, 817, 957
Hin6I	GCGC	2 cut(s)	210, 339
HinP1I	GCGC	2 cut(s)	210, 339
HincII	GTYRAC	3 cut(s)	91, 819, 922
HindII	GTYRAC	3 cut(s)	91, 819, 922
HinfI	GANTC	1 cut(s)	898
HpaII	CCGG	5 cut(s)	258, 410, 470, 822, 843
HphI	GGTGA	3 cut(s)	298, 317, 1102
Hpy166II	GTNNAC	7 cut(s)	91, 187, 268, 442, 526, 819, 922
Hpy188I	TCNGA	8 cut(s)	131, 153, 245, 435, 578, 772, 897, 941
Hpy188III	TCNNGA	4 cut(s)	100, 196, 554, 568
Hpy8I	GTNNAC	7 cut(s)	91, 187, 268, 442, 526, 819, 922
HpyAV	CCTTC	3 cut(s)	485, 796, 1030
HpyCH4III	ACNGT	5 cut(s)	345, 565, 878, 889, 1019
HpyCH4IV	ACGT	3 cut(s)	477, 924, 996
HpyCH4V	TGCA	4 cut(s)	24, 357, 442, 1058
HpyF3I	CTNAG	1 cut(s)	1032
HpySE526I	ACGT	3 cut(s)	477, 924, 996
Hsp92II	CATG	8 cut(s)	34, 70, 205, 251, 419, 766, 817, 957
HspAI	GCGC	2 cut(s)	210, 339
Ksp22I	TGATCA	1 cut(s)	570
Kzo9I	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
LmnI	GCTCC	3 cut(s)	123, 279, 578
Lsp1109I	GCAGC	4 cut(s)	58, 323, 892, 895
LweI	GCATC	1 cut(s)	339
MaeI	CTAG	2 cut(s)	668, 912
MaeII	ACGT	3 cut(s)	477, 924, 996
MaeIII	GTNAC	2 cut(s)	305, 781
MalI	GATC	6 cut(s)	104, 155, 263, 453, 572, 767
MbiI	CCGCTC	1 cut(s)	614
MboI	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
MboII	GAAGA	2 cut(s)	1000, 1058
MflI	RGATCY	2 cut(s)	102, 451
MhlI	GDGCHC	4 cut(s)	444, 510, 631, 1069
MluCI	AATT	5 cut(s)	147, 464, 636, 750, 1100
MlyI	GAGTC	1 cut(s)	892
MmeI	TCCRAC	1 cut(s)	910
MroXI	GAANNNNTTC	1 cut(s)	395
MseI	TTAA	3 cut(s)	225, 462, 753
MslI	CAYNNNNRTG	2 cut(s)	252, 1088
MspA1I	CMGCKG	2 cut(s)	336, 905
MspI	CCGG	5 cut(s)	258, 410, 470, 822, 843
MspR9I	CCNGG	4 cut(s)	259, 303, 327, 843
MvaI	CCWGG	2 cut(s)	303, 327
MvnI	CGCG	2 cut(s)	212, 312
NciI	CCSGG	2 cut(s)	259, 843
NdeII	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
NlaIII	CATG	8 cut(s)	34, 70, 205, 251, 419, 766, 817, 957
NlaIV	GGNNCC	7 cut(s)	133, 300, 347, 421, 553, 630, 891
NmuCI	GTSAC	2 cut(s)	305, 781
NsbI	TGCGCA	1 cut(s)	340
NspV	TTCGAA	1 cut(s)	145
PaeR7I	CTCGAG	1 cut(s)	968
PdmI	GAANNNNTTC	1 cut(s)	395
PinAI	ACCGGT	1 cut(s)	409
PkrI	GCNGC	8 cut(s)	73, 338, 368, 613, 907, 910, 960, 1005
PleI	GAGTC	1 cut(s)	892
PpsI	GAGTC	1 cut(s)	892
PshAI	GACNNNNGTC	2 cut(s)	563, 663
Psp6I	CCWGG	2 cut(s)	301, 325
PspEI	GGTNACC	1 cut(s)	305
PspGI	CCWGG	2 cut(s)	301, 325
PspN4I	GGNNCC	7 cut(s)	133, 300, 347, 421, 553, 630, 891
PspPI	GGNCC	3 cut(s)	131, 551, 584
PspXI	VCTCGAGB	1 cut(s)	968
PsuI	RGATCY	2 cut(s)	102, 451
PvuII	CAGCTG	1 cut(s)	336
RseI	CAYNNNNRTG	2 cut(s)	252, 1088
SalI	GTCGAC	1 cut(s)	817
SaqAI	TTAA	3 cut(s)	225, 462, 753
SatI	GCNGC	8 cut(s)	72, 337, 367, 612, 906, 909, 959, 1004
Sau3AI	GATC	6 cut(s)	102, 153, 261, 451, 570, 765
Sau96I	GGNCC	3 cut(s)	131, 551, 584
SchI	GAGTC	1 cut(s)	892
ScrFI	CCNGG	4 cut(s)	259, 303, 327, 843
SduI	GDGCHC	4 cut(s)	444, 510, 631, 1069
SfaNI	GCATC	1 cut(s)	339
Sfr274I	CTCGAG	1 cut(s)	968
SfuI	TTCGAA	1 cut(s)	145
SinI	GGWCC	2 cut(s)	131, 551
SlaI	CTCGAG	1 cut(s)	968
SmiMI	CAYNNNNRTG	2 cut(s)	252, 1088
SmlI	CTYRAG	3 cut(s)	194, 511, 968
SmoI	CTYRAG	3 cut(s)	194, 511, 968
Sse9I	AATT	5 cut(s)	147, 464, 636, 750, 1100
SspMI	CTAG	2 cut(s)	668, 912
StyD4I	CCNGG	4 cut(s)	257, 301, 325, 841
StyI	CCWWGG	1 cut(s)	423
TaaI	ACNGT	5 cut(s)	345, 565, 878, 889, 1019
TaiI	ACGT	3 cut(s)	480, 927, 999
TaqI	TCGA	5 cut(s)	145, 713, 789, 818, 969
TasI	AATT	5 cut(s)	147, 464, 636, 750, 1100
TauI	GCSGC	4 cut(s)	369, 614, 961, 1006
Tru1I	TTAA	3 cut(s)	225, 462, 753
Tru9I	TTAA	3 cut(s)	225, 462, 753
TscAI	CASTG	1 cut(s)	359
TseFI	GTSAC	2 cut(s)	305, 781
TseI	GCWGC	4 cut(s)	71, 336, 905, 908
Tsp45I	GTSAC	2 cut(s)	305, 781
TspDTI	ATGAA	5 cut(s)	218, 404, 404, 711, 753
TspRI	CASTG	1 cut(s)	359
VneI	GTGCAC	1 cut(s)	440
VpaK11BI	GGWCC	2 cut(s)	131, 551
XapI	RAATTY	1 cut(s)	147
XcmI	CCANNNNNNNNNTGG	1 cut(s)	23
XhoI	CTCGAG	1 cut(s)	968
XmiI	GTMKAC	2 cut(s)	267, 818
XmnI	GAANNNNTTC	1 cut(s)	395
XspI	CTAG	2 cut(s)	668, 912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.