Rroxscaffold_4G00282010

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001681
Physical Location & Seq
Reverse (-)
4173839 .. 4176254
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282010.1

Sequence Viewer

Length: 1071 bp
ATGGGTAAGGGAGGGACCATGGAGGAGAACATGGCTGCTTGGCTTGTTGGTATCAACACCCTCAAGATTCAACCTTTCAAGCTCCCTGATCTTGGACCTCATGATGTTAGGATTCGGATGAAGGCTGTTGGGATATGTGGGAGTGATGTTCACTACCTCAAGGCCATGAGGTGTGCAGATTTTATAGTTAAAGAGCCAATGGTAATTGGGCATGAGTGTGCTGGGATCATAGAGGAAGTTGGGGCTGAAGTCAAGCATCTGGCACCTGGTGACCGCGTGGCACTAGAGCCCGGTATCAGTTGCTGGCGATGTGAGTCCTGCAAAGAAGGTCGCTACAATCTATGCCCGGATATGAAGTTTTTCGCCACTCCACCAGTTCATGGTTCACTGGCAAACCAGGTTGTCCATCCTGCAGACCTGTGTTTTAAATTGCCAGAGAATGTGAGCTTGGAGGAAGGGGCAATGTGTGAGCCCTTGAGTGTTGGTGTTCATGCTTGTCGACGAGCTAATGTTGGCCCAGAAACAAATGTTTTGGTCATGGGAGCAGGACCCATAGGGCTTGTTACTCTGCTAGCTGCTCGCGCTTTTGGGGCACCCAGAATTGTCATTGTGGACGTGGATGACCACCGTTTATCAGTTGCAAAGACTCTTGGTGCAGATGAGATCGTAAAAGTTTCAACAAACATCCAGGATGTAGCTGAAGAAGTGGTTCAGATAGGTAAAGCTATGGGAGCTGGAGTGGACGTGACCTTTGATTGTGTAGGCTTTGATAAAACCATGTCAACAGGTCTGCGTGCTACTCGTCCTGGTGGCAAAGTTTGCCTTGTGGGAATGGGTCACGATGCGATGACTCTCCCACTTACTTCAGCTTCTGCCAGGGAGGTAGATGTGATTGGAATTTTCCGATACAAGAACACATGGCCTCTATGCCTAGAGTTTTTGAGAAGTGGTAAGATCGATGTGAAGCCGCTCATAACTCATCGGTTTGGGTTTTCTCAAAAGGAGGTGGAAGACGCCTTCGCAACCAGTGCTCGTGGAGGCAATGCCATTAAGGTCATGTTTAATCTGTAA

Protein Analysis

356

Amino Acids

38.25

Weight (kDa)

6.37

Isoelectric Point (pI)

24.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 32 - 146 2.1e-29 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 153 - 282 2.7e-10 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 184 - 314 3.3e-28 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 217 - 347 1.3e-08 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 262, 592
AccB7I CCANNNNNTGG 1 cut(s) 380
AccBSI CCGCTC 1 cut(s) 970
AccI GTMKAC 1 cut(s) 499
AccII CGCG 2 cut(s) 276, 582
AciI CCGC 2 cut(s) 274, 968
AclWI GGATC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 897
AcuI CTGAAG 3 cut(s) 267, 720, 849
AcyI GRCGYC 1 cut(s) 1014
AdeI CACNNNGTG 1 cut(s) 269
AfiI CCNNNNNNNGG 2 cut(s) 92, 380
AgsI TTSAA 3 cut(s) 71, 79, 678
AjiI CACGTC 2 cut(s) 616, 745
AjnI CCWGG 5 cut(s) 265, 396, 687, 805, 875
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 8 cut(s) 82, 447, 506, 575, 698, 725, 734, 869
AluI AGCT 8 cut(s) 82, 447, 506, 575, 698, 725, 734, 869
Alw21I GWGCWC 1 cut(s) 1033
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 2 cut(s) 303, 872
AoxI GGCC 3 cut(s) 162, 514, 920
ApeKI GCWGC 2 cut(s) 35, 575
ApoI RAATTY 1 cut(s) 897
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
Asp700I GAANNNNTTC 2 cut(s) 359, 708
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 4 cut(s) 15, 95, 515, 548
AsuC2I CCSGG 2 cut(s) 291, 347
AsuHPI GGTGA 1 cut(s) 281
AsuNHI GCTAGC 1 cut(s) 571
AvaII GGWCC 3 cut(s) 15, 95, 548
BaeGI GKGCMC 1 cut(s) 595
BanI GGYRCC 2 cut(s) 262, 592
BanII GRGCYC 2 cut(s) 291, 474
BauI CACGAG 1 cut(s) 1032
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 1033
BbvI GCAGC 2 cut(s) 22, 562
BccI CCATC 1 cut(s) 414
BciT130I CCWGG 5 cut(s) 267, 398, 689, 807, 877
BcnI CCSGG 2 cut(s) 291, 347
BfaI CTAG 3 cut(s) 284, 572, 932
BfmI CTRYAG 1 cut(s) 411
BisI GCNGC 3 cut(s) 36, 576, 968
BlsI GCNGC 3 cut(s) 37, 577, 969
Bme1390I CCNGG 7 cut(s) 267, 291, 347, 398, 689, 807, 877
Bme18I GGWCC 3 cut(s) 15, 95, 548
BmgBI CACGTC 2 cut(s) 616, 745
BmgT120I GGNCC 4 cut(s) 15, 95, 515, 548
BmiI GGNNCC 4 cut(s) 16, 264, 550, 594
BmrFI CCNGG 7 cut(s) 267, 291, 347, 398, 689, 807, 877
BmsI GCATC 2 cut(s) 265, 832
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 1017
BpmI CTGGAG 1 cut(s) 756
BpuEI CTTGAG 3 cut(s) 47, 143, 496
BpuMI CCSGG 2 cut(s) 291, 347
Bsa29I ATCGAT 1 cut(s) 957
BsaHI GRCGYC 1 cut(s) 1014
BsaJI CCNNGG 2 cut(s) 18, 876
BsaXI ACNNNNNCTCC 4 cut(s) 723, 729, 753, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 380
Bse1I ACTGG 3 cut(s) 374, 393, 1026
Bse3DI GCAATG 2 cut(s) 468, 1048
BseBI CCWGG 5 cut(s) 267, 398, 689, 807, 877
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 2 cut(s) 18, 876
BseGI GGATG 5 cut(s) 123, 406, 625, 684, 697
BseLI CCNNNNNNNGG 2 cut(s) 92, 380
BseMI GCAATG 2 cut(s) 468, 1048
BseNI ACTGG 3 cut(s) 374, 393, 1026
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 2 cut(s) 22, 562
BseYI CCCAGC 1 cut(s) 221
BsgI GTGCAG 2 cut(s) 195, 675
Bsh1236I CGCG 2 cut(s) 276, 582
BshFI GGCC 3 cut(s) 164, 516, 922
BshNI GGYRCC 2 cut(s) 262, 592
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 1033
BsiSI CCGG 2 cut(s) 291, 347
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 2 cut(s) 92, 380
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 3 cut(s) 164, 516, 922
Bsp1286I GDGCHC 4 cut(s) 291, 474, 595, 1033
Bsp143I GATC 4 cut(s) 88, 225, 663, 954
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 2 cut(s) 274, 968
BspANI GGCC 3 cut(s) 164, 516, 922
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 2 cut(s) 276, 582
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 16, 264, 550, 594
BspMAI CTGCAG 1 cut(s) 415
BspOI GCTAGC 1 cut(s) 575
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 2 cut(s) 262, 592
BsrBI CCGCTC 1 cut(s) 970
BsrDI GCAATG 2 cut(s) 468, 1048
BsrI ACTGG 3 cut(s) 374, 393, 1026
BssECI CCNNGG 2 cut(s) 18, 876
BssMI GATC 4 cut(s) 88, 225, 663, 954
BssNI GRCGYC 1 cut(s) 1014
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 5 cut(s) 267, 398, 689, 807, 877
Bst4CI ACNGT 1 cut(s) 629
BstACI GRCGYC 1 cut(s) 1014
BstAPI GCANNNNNTGC 2 cut(s) 819, 1028
BstC8I GCNNGC 4 cut(s) 305, 573, 580, 795
BstDSI CCRYGG 1 cut(s) 18
BstEII GGTNACC 1 cut(s) 269
BstF5I GGATG 5 cut(s) 123, 406, 625, 684, 697
BstFNI CGCG 2 cut(s) 276, 582
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 4 cut(s) 91, 228, 666, 957
BstMBI GATC 4 cut(s) 88, 225, 663, 954
BstMWI GCNNNNNNNGC 5 cut(s) 581, 590, 731, 819, 1028
BstNI CCWGG 5 cut(s) 267, 398, 689, 807, 877
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 7 cut(s) 265, 289, 345, 396, 687, 805, 875
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 2 cut(s) 276, 582
BstV1I GCAGC 2 cut(s) 22, 562
BstV2I GAAGAC 1 cut(s) 1017
Bsu15I ATCGAT 1 cut(s) 957
BsuRI GGCC 3 cut(s) 164, 516, 922
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 1 cut(s) 18
BtgZI GCGATG 2 cut(s) 322, 860
BtrI CACGTC 2 cut(s) 616, 745
BtsCI GGATG 5 cut(s) 123, 406, 625, 684, 697
BtsIMutI CAGTG 2 cut(s) 386, 1033
Cac8I GCNNGC 4 cut(s) 305, 573, 580, 795
CaiI CAGNNNCTG 2 cut(s) 303, 872
CciI TCATGA 1 cut(s) 100
CfoI GCGC 1 cut(s) 584
Cfr13I GGNCC 4 cut(s) 15, 95, 515, 548
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 1022
CsiI ACCWGGT 2 cut(s) 265, 396
DpnI GATC 4 cut(s) 90, 227, 665, 956
DpnII GATC 4 cut(s) 88, 225, 663, 954
DraI TTTAAA 1 cut(s) 427
DraIII CACNNNGTG 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 291, 474
Eco47I GGWCC 3 cut(s) 15, 95, 548
Eco57I CTGAAG 3 cut(s) 267, 720, 849
Eco91I GGTNACC 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 548
EcoO65I GGTNACC 1 cut(s) 269
EcoRII CCWGG 5 cut(s) 265, 396, 687, 805, 875
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 291, 474
ErhI CCWWGG 1 cut(s) 18
FalI AAGNNNNNCTT 2 cut(s) 807, 839
FaqI GGGAC 1 cut(s) 28
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 3 cut(s) 36, 576, 968
FokI GGATG 5 cut(s) 130, 393, 632, 671, 704
FriOI GRGCYC 2 cut(s) 291, 474
Fsp4HI GCNGC 3 cut(s) 36, 576, 968
FspBI CTAG 3 cut(s) 284, 572, 932
GlaI GCGC 1 cut(s) 583
GluI GCNGC 3 cut(s) 36, 576, 968
GsaI CCCAGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 3 cut(s) 164, 516, 922
HapII CCGG 2 cut(s) 291, 347
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 1 cut(s) 584
Hin1I GRCGYC 1 cut(s) 1014
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HincII GTYRAC 2 cut(s) 500, 783
HindII GTYRAC 2 cut(s) 500, 783
HinfI GANTC 5 cut(s) 67, 112, 314, 646, 850
HpaII CCGG 2 cut(s) 291, 347
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 6 cut(s) 151, 386, 500, 613, 742, 783
Hpy188I TCNGA 3 cut(s) 117, 714, 905
Hpy188III TCNNGA 3 cut(s) 64, 101, 839
Hpy8I GTNNAC 6 cut(s) 151, 386, 500, 613, 742, 783
Hpy99I CGWCG 1 cut(s) 504
HpyAV CCTTC 4 cut(s) 115, 320, 449, 1027
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 2 cut(s) 615, 744
HpyCH4V TGCA 5 cut(s) 176, 321, 413, 641, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 581, 590, 731, 819, 1028
HpySE526I ACGT 2 cut(s) 615, 744
Hsp92I GRCGYC 1 cut(s) 1014
HspAI GCGC 1 cut(s) 582
Kzo9I GATC 4 cut(s) 88, 225, 663, 954
LmnI GCTCC 3 cut(s) 87, 542, 731
Lsp1109I GCAGC 2 cut(s) 22, 562
LweI GCATC 2 cut(s) 265, 832
MabI ACCWGGT 2 cut(s) 265, 396
MaeI CTAG 3 cut(s) 284, 572, 932
MaeII ACGT 2 cut(s) 615, 744
MaeIII GTNAC 4 cut(s) 269, 562, 745, 836
MalI GATC 4 cut(s) 90, 227, 665, 956
MbiI CCGCTC 1 cut(s) 970
MboI GATC 4 cut(s) 88, 225, 663, 954
MboII GAAGA 2 cut(s) 713, 1022
MhlI GDGCHC 4 cut(s) 291, 474, 595, 1033
MluCI AATT 4 cut(s) 204, 428, 600, 897
MlyI GAGTC 3 cut(s) 323, 640, 844
MroXI GAANNNNTTC 2 cut(s) 359, 708
MseI TTAA 4 cut(s) 189, 426, 1050, 1062
MslI CAYNNNNRTG 1 cut(s) 216
MspI CCGG 2 cut(s) 291, 347
MspR9I CCNGG 7 cut(s) 267, 291, 347, 398, 689, 807, 877
MvaI CCWGG 5 cut(s) 267, 398, 689, 807, 877
MvnI CGCG 2 cut(s) 276, 582
MwoI GCNNNNNNNGC 5 cut(s) 581, 590, 731, 819, 1028
NciI CCSGG 2 cut(s) 291, 347
NcoI CCATGG 1 cut(s) 18
NdeII GATC 4 cut(s) 88, 225, 663, 954
NheI GCTAGC 1 cut(s) 571
NlaIV GGNNCC 4 cut(s) 16, 264, 550, 594
NmuCI GTSAC 3 cut(s) 269, 745, 836
PagI TCATGA 1 cut(s) 100
PdmI GAANNNNTTC 2 cut(s) 359, 708
PfeI GAWTC 2 cut(s) 67, 112
PflMI CCANNNNNTGG 1 cut(s) 380
PfoI TCCNGGA 1 cut(s) 687
PkrI GCNGC 3 cut(s) 37, 577, 969
PleI GAGTC 3 cut(s) 322, 640, 844
PpsI GAGTC 3 cut(s) 322, 640, 844
PpuMI RGGWCCY 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 5 cut(s) 265, 396, 687, 805, 875
PspEI GGTNACC 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 5 cut(s) 265, 396, 687, 805, 875
PspN4I GGNNCC 4 cut(s) 16, 264, 550, 594
PspPI GGNCC 4 cut(s) 15, 95, 515, 548
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 2 cut(s) 303, 872
RseI CAYNNNNRTG 1 cut(s) 216
SalI GTCGAC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 189, 426, 1050, 1062
SatI GCNGC 3 cut(s) 36, 576, 968
Sau3AI GATC 4 cut(s) 88, 225, 663, 954
Sau96I GGNCC 4 cut(s) 15, 95, 515, 548
SchI GAGTC 3 cut(s) 323, 640, 844
ScrFI CCNGG 7 cut(s) 267, 291, 347, 398, 689, 807, 877
SduI GDGCHC 4 cut(s) 291, 474, 595, 1033
SexAI ACCWGGT 2 cut(s) 265, 396
SfaNI GCATC 2 cut(s) 265, 832
SfcI CTRYAG 1 cut(s) 411
SinI GGWCC 3 cut(s) 15, 95, 548
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 3 cut(s) 62, 158, 475
SmoI CTYRAG 3 cut(s) 62, 158, 475
Sse9I AATT 4 cut(s) 204, 428, 600, 897
SsiI CCGC 2 cut(s) 274, 968
SspMI CTAG 3 cut(s) 284, 572, 932
StyD4I CCNGG 7 cut(s) 265, 289, 345, 396, 687, 805, 875
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 2 cut(s) 618, 747
TaqI TCGA 2 cut(s) 499, 957
TasI AATT 4 cut(s) 204, 428, 600, 897
TauI GCSGC 1 cut(s) 970
TfiI GAWTC 2 cut(s) 67, 112
Tru1I TTAA 4 cut(s) 189, 426, 1050, 1062
Tru9I TTAA 4 cut(s) 189, 426, 1050, 1062
TscAI CASTG 2 cut(s) 393, 1033
TseFI GTSAC 3 cut(s) 269, 745, 836
TseI GCWGC 2 cut(s) 35, 575
Tsp45I GTSAC 3 cut(s) 269, 745, 836
TspDTI ATGAA 4 cut(s) 134, 368, 368, 479
TspRI CASTG 2 cut(s) 393, 1033
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 3 cut(s) 15, 95, 548
XapI RAATTY 1 cut(s) 897
XmiI GTMKAC 1 cut(s) 499
XmnI GAANNNNTTC 2 cut(s) 359, 708
XspI CTAG 3 cut(s) 284, 572, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.