pycom07g24090

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001681
Physical Location & Seq
Forward (+)
24887554 .. 24889084
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24090.3

Sequence Viewer

Length: 1068 bp
ATGATCTACGAAGGGTTGGGACCCCATGATGTTCGAATTCGGATCAAGGCTGTCGGCATTTGTGGAAGCGATGTCCACTACCTCAAGACCATGAAATGTGCGGATTTTGAGGTGAAAGAGCCAATGGTAATGGGACATGAGTGTGCCGGGATAGTAGACAAAGTTGGGAGCGAGGTGAAGCATTTGGTGCCTGGGGACCGTGTGGCGGTAGAGCCCGGCATCAGCTGCGCACGGTGCCGGCAGTGCAAAGGAGGCCGCTATAATCTTTGCCCTGATATGAAGTTTTTCGCCACGCCACCGGTTCATGGTTCCTTGGCTAATCAGATTGTGCACCCTGCGGATCTGTGCTTTAAATTGCCGGAAAACGTGAGCTTGGAGGAAGGGGCAATGTGTGAGCCGTTGAGTGTTGGGGTTCACGCTTGTCGGCGAGCCAATGTTGGTCCCGAAACAACTGTTCTGATCATCGGAGCAGGGCCTATTGGTCTCGTTTCAGTTTTGGCCGCTCGTGCTTTTGGGGCTCCAAGAATTGTGATTGTGGATATGGATGACAAGCGTCTAGCAATGGCAAAGTCTCTCGGTGCTGATGAAACAGTGAAAGTTTCGACAAAAATGGAGGATTTAGATGATGAAGTTGCAGAAATTAAAAAAGCCATGATCTCCGATGTATGTTACCTTCGATTGTGTGGGTTTCAACAAAACCATGTCGACCGGCCTCAATGCTACTCGTCCGGGACGGTGCCTCTTACTCCCGCTGCTGCTAGGGAGGTTGACGTTGTTGGAGTTTTCCGATACCAGAACACATGGCCGCTTTGCCTCGAGTTTTTGAGAAGTGGGAAGATCGATGTGAAGCCTCTTATCACGCACCGTTTTGGATTTACTGAGAAGGAGGTGGAAGAAGCGTTTGCGACAAGTGCTCGTGGAGGTAACGCCATCAAAGTGATGTTCAAATTGCAAAGACAAAGACAGGTTTTGATGATCAAATTCTTAGCAGAATGTAGAGTTTTTTTTTTCTTTTTTTTTTTTTGTGGGTGGAGAGGGGGGAGGGGGAGGATTATTAACATGTCATAA

Protein Analysis

356

Amino Acids

39.18

Weight (kDa)

8.43

Isoelectric Point (pI)

25.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 229
AccB1I GGYRCC 3 cut(s) 187, 234, 736
AccBSI CCGCTC 1 cut(s) 503
AccI GTMKAC 2 cut(s) 156, 705
AciI CCGC 7 cut(s) 101, 206, 256, 338, 501, 750, 806
AclWI GGATC 2 cut(s) 50, 348
AcoI YGGCCR 2 cut(s) 498, 803
AcsI RAATTY 2 cut(s) 36, 980
AfiI CCNNNNNNNGG 2 cut(s) 205, 305
AflIII ACRYGT 1 cut(s) 1059
AgeI ACCGGT 1 cut(s) 298
AgsI TTSAA 2 cut(s) 692, 946
AjnI CCWGG 1 cut(s) 190
AluBI AGCT 2 cut(s) 225, 372
AluI AGCT 2 cut(s) 225, 372
Alw21I GWGCWC 2 cut(s) 333, 916
Alw26I GTCTC 2 cut(s) 488, 576
Alw44I GTGCAC 1 cut(s) 329
AlwI GGATC 2 cut(s) 50, 348
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 5 cut(s) 253, 473, 498, 710, 803
ApaLI GTGCAC 1 cut(s) 329
ApeKI GCWGC 3 cut(s) 225, 752, 755
ApoI RAATTY 2 cut(s) 36, 980
AsiGI ACCGGT 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 4 cut(s) 20, 196, 440, 473
AsuC2I CCSGG 3 cut(s) 148, 216, 730
AsuHPI GGTGA 2 cut(s) 124, 187
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 3 cut(s) 20, 196, 440
BaeGI GKGCMC 1 cut(s) 333
BanI GGYRCC 3 cut(s) 187, 234, 736
BanII GRGCYC 2 cut(s) 216, 520
BarI GAAGNNNNNNTAC 2 cut(s) 657, 689
BauI CACGAG 2 cut(s) 504, 915
Bbv12I GWGCWC 2 cut(s) 333, 916
BbvI GCAGC 3 cut(s) 212, 739, 742
BccI CCATC 1 cut(s) 938
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 192
BclI TGATCA 2 cut(s) 459, 975
BcnI CCSGG 3 cut(s) 148, 216, 730
BcoDI GTCTC 2 cut(s) 488, 576
BfaI CTAG 2 cut(s) 557, 759
BisI GCNGC 6 cut(s) 226, 256, 501, 753, 756, 806
BlsI GCNGC 6 cut(s) 227, 257, 502, 754, 757, 807
Bme1390I CCNGG 4 cut(s) 148, 192, 216, 730
Bme18I GGWCC 3 cut(s) 20, 196, 440
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 4 cut(s) 20, 196, 440, 473
BmiI GGNNCC 9 cut(s) 21, 22, 189, 197, 236, 310, 442, 519, 738
BmrFI CCNGG 4 cut(s) 148, 192, 216, 730
BmsI GCATC 1 cut(s) 228
BoxI GACNNNNGTC 1 cut(s) 552
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 68
BpuMI CCSGG 3 cut(s) 148, 216, 730
Bsa29I ATCGAT 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 2 cut(s) 191, 312
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 305
Bse118I RCCGGY 3 cut(s) 237, 298, 708
Bse3DI GCAATG 2 cut(s) 393, 567
BseBI CCWGG 1 cut(s) 192
BseCI ATCGAT 1 cut(s) 840
BseDI CCNNGG 2 cut(s) 191, 312
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 2 cut(s) 205, 305
BseMI GCAATG 2 cut(s) 393, 567
BseMII CTCAG 1 cut(s) 870
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 3 cut(s) 212, 739, 742
Bsh1285I CGRYCG 1 cut(s) 709
BshFI GGCC 5 cut(s) 255, 475, 500, 712, 805
BshNI GGYRCC 3 cut(s) 187, 234, 736
BshTI ACCGGT 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 840
BsiEI CGRYCG 1 cut(s) 709
BsiHKAI GWGCWC 2 cut(s) 333, 916
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 7 cut(s) 147, 216, 238, 299, 359, 709, 729
BslFI GGGAC 5 cut(s) 33, 147, 209, 426, 745
BslI CCNNNNNNNGG 2 cut(s) 205, 305
BsmAI GTCTC 2 cut(s) 488, 576
BsmFI GGGAC 5 cut(s) 33, 147, 209, 426, 745
BsnI GGCC 5 cut(s) 255, 475, 500, 712, 805
Bso31I GGTCTC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 4 cut(s) 216, 333, 520, 916
Bsp143I GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
BspACI CCGC 7 cut(s) 101, 206, 256, 338, 501, 750, 806
BspANI GGCC 5 cut(s) 255, 475, 500, 712, 805
BspCNI CTCAG 1 cut(s) 871
BspDI ATCGAT 1 cut(s) 840
BspLI GGNNCC 9 cut(s) 21, 22, 189, 197, 236, 310, 442, 519, 738
BspPI GGATC 2 cut(s) 50, 348
BspT104I TTCGAA 1 cut(s) 34
BspT107I GGYRCC 3 cut(s) 187, 234, 736
BspTNI GGTCTC 1 cut(s) 488
BsrBI CCGCTC 1 cut(s) 503
BsrDI GCAATG 2 cut(s) 393, 567
BsrFI RCCGGY 3 cut(s) 237, 298, 708
BssAI RCCGGY 3 cut(s) 237, 298, 708
BssECI CCNNGG 2 cut(s) 191, 312
BssMI GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
BssSI CACGAG 2 cut(s) 504, 915
BssT1I CCWWGG 1 cut(s) 312
Bst2BI CACGAG 2 cut(s) 504, 915
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 6 cut(s) 200, 234, 454, 592, 736, 866
BstAPI GCANNNNNTGC 2 cut(s) 187, 225
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 239, 429
BstDEI CTNAG 2 cut(s) 879, 985
BstF5I GGATG 1 cut(s) 550
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 7 cut(s) 6, 45, 343, 462, 657, 840, 978
BstMAI GTCTC 2 cut(s) 488, 576
BstMBI GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
BstMCI CGRYCG 1 cut(s) 709
BstMWI GCNNNNNNNGC 8 cut(s) 187, 225, 234, 243, 252, 506, 515, 911
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 1063
BstPAI GACNNNNGTC 1 cut(s) 552
BstSCI CCNGG 4 cut(s) 146, 190, 214, 728
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 212, 739, 742
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 840
BsuRI GGCC 5 cut(s) 255, 475, 500, 712, 805
BsuTUI ATCGAT 1 cut(s) 840
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 2 cut(s) 248, 597
Cac8I GCNNGC 2 cut(s) 239, 429
CfoI GCGC 1 cut(s) 230
Cfr10I RCCGGY 3 cut(s) 237, 298, 708
Cfr13I GGNCC 4 cut(s) 20, 196, 440, 473
ClaI ATCGAT 1 cut(s) 840
CseI GACGC 1 cut(s) 542
CspAI ACCGGT 1 cut(s) 298
CviAII CATG 8 cut(s) 26, 91, 137, 305, 652, 701, 801, 1060
DdeI CTNAG 2 cut(s) 879, 985
DpnI GATC 7 cut(s) 5, 44, 342, 461, 656, 839, 977
DpnII GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
DraI TTTAAA 1 cut(s) 352
EaeI YGGCCR 2 cut(s) 498, 803
Eco130I CCWWGG 1 cut(s) 312
Eco24I GRGCYC 2 cut(s) 216, 520
Eco31I GGTCTC 1 cut(s) 488
Eco47I GGWCC 3 cut(s) 20, 196, 440
Eco88I CYCGRG 1 cut(s) 815
EcoO109I RGGNCCY 2 cut(s) 20, 473
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 312
EcoT38I GRGCYC 2 cut(s) 216, 520
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 8 cut(s) 29, 94, 140, 308, 655, 704, 804, 1063
FaqI GGGAC 5 cut(s) 33, 147, 209, 426, 745
FatI CATG 8 cut(s) 25, 90, 136, 304, 651, 700, 800, 1059
FauI CCCGC 1 cut(s) 757
FbaI TGATCA 2 cut(s) 459, 975
FblI GTMKAC 2 cut(s) 156, 705
Fnu4HI GCNGC 6 cut(s) 226, 256, 501, 753, 756, 806
FokI GGATG 1 cut(s) 557
FriOI GRGCYC 2 cut(s) 216, 520
Fsp4HI GCNGC 6 cut(s) 226, 256, 501, 753, 756, 806
FspBI CTAG 2 cut(s) 557, 759
FspI TGCGCA 1 cut(s) 229
GlaI GCGC 1 cut(s) 229
GluI GCNGC 6 cut(s) 226, 256, 501, 753, 756, 806
HaeIII GGCC 5 cut(s) 255, 475, 500, 712, 805
HapII CCGG 7 cut(s) 147, 216, 238, 299, 359, 709, 729
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 230
Hin1II CATG 8 cut(s) 29, 94, 140, 308, 655, 704, 804, 1063
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 2 cut(s) 706, 769
HindII GTYRAC 2 cut(s) 706, 769
HpaII CCGG 7 cut(s) 147, 216, 238, 299, 359, 709, 729
HphI GGTGA 2 cut(s) 124, 187
Hpy166II GTNNAC 6 cut(s) 76, 157, 331, 415, 706, 769
Hpy188I TCNGA 6 cut(s) 42, 324, 459, 467, 661, 788
Hpy188III TCNNGA 2 cut(s) 85, 443
Hpy8I GTNNAC 6 cut(s) 76, 157, 331, 415, 706, 769
HpyAV CCTTC 4 cut(s) 5, 374, 683, 877
HpyCH4III ACNGT 6 cut(s) 200, 234, 454, 592, 736, 866
HpyCH4IV ACGT 2 cut(s) 366, 771
HpyCH4V TGCA 4 cut(s) 246, 331, 635, 952
HpyF10VI GCNNNNNNNGC 8 cut(s) 187, 225, 234, 243, 252, 506, 515, 911
HpyF3I CTNAG 2 cut(s) 879, 985
HpySE526I ACGT 2 cut(s) 366, 771
Hsp92II CATG 8 cut(s) 29, 94, 140, 308, 655, 704, 804, 1063
HspAI GCGC 1 cut(s) 228
KflI GGGWCCC 1 cut(s) 20
KroI GCCGGC 1 cut(s) 237
KroNI GCCGGC 1 cut(s) 239
Ksp22I TGATCA 2 cut(s) 459, 975
Kzo9I GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
LmnI GCTCC 3 cut(s) 168, 467, 523
Lsp1109I GCAGC 3 cut(s) 212, 739, 742
LweI GCATC 1 cut(s) 228
MaeI CTAG 2 cut(s) 557, 759
MaeII ACGT 2 cut(s) 366, 771
MaeIII GTNAC 2 cut(s) 668, 923
MalI GATC 7 cut(s) 5, 44, 342, 461, 656, 839, 977
MbiI CCGCTC 1 cut(s) 503
MboI GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
MboII GAAGA 2 cut(s) 847, 905
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 4 cut(s) 216, 333, 520, 916
MluCI AATT 6 cut(s) 36, 353, 525, 639, 947, 980
MmeI TCCRAC 1 cut(s) 757
MroNI GCCGGC 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 3 cut(s) 351, 642, 1056
MslI CAYNNNNRTG 2 cut(s) 141, 935
MspA1I CMGCKG 2 cut(s) 225, 752
MspI CCGG 7 cut(s) 147, 216, 238, 299, 359, 709, 729
MspR9I CCNGG 4 cut(s) 148, 192, 216, 730
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 8 cut(s) 187, 225, 234, 243, 252, 506, 515, 911
NaeI GCCGGC 1 cut(s) 239
NciI CCSGG 3 cut(s) 148, 216, 730
NdeII GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
NgoMIV GCCGGC 1 cut(s) 237
NlaIII CATG 8 cut(s) 29, 94, 140, 308, 655, 704, 804, 1063
NlaIV GGNNCC 9 cut(s) 21, 22, 189, 197, 236, 310, 442, 519, 738
NsbI TGCGCA 1 cut(s) 229
NspI RCATGY 1 cut(s) 1063
NspV TTCGAA 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 815
PciI ACATGT 1 cut(s) 1059
PdiI GCCGGC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 728
PinAI ACCGGT 1 cut(s) 298
PkrI GCNGC 6 cut(s) 227, 257, 502, 754, 757, 807
PpuMI RGGWCCY 1 cut(s) 20
PscI ACATGT 1 cut(s) 1059
PshAI GACNNNNGTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 9 cut(s) 21, 22, 189, 197, 236, 310, 442, 519, 738
PspPI GGNCC 4 cut(s) 20, 196, 440, 473
PspPPI RGGWCCY 1 cut(s) 20
PspXI VCTCGAGB 1 cut(s) 815
PsuI RGATCY 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 225
RseI CAYNNNNRTG 2 cut(s) 141, 935
SalI GTCGAC 1 cut(s) 704
SaqAI TTAA 3 cut(s) 351, 642, 1056
SatI GCNGC 6 cut(s) 226, 256, 501, 753, 756, 806
Sau3AI GATC 7 cut(s) 3, 42, 340, 459, 654, 837, 975
Sau96I GGNCC 4 cut(s) 20, 196, 440, 473
ScrFI CCNGG 4 cut(s) 148, 192, 216, 730
SduI GDGCHC 4 cut(s) 216, 333, 520, 916
SfaNI GCATC 1 cut(s) 228
Sfr274I CTCGAG 1 cut(s) 815
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 3 cut(s) 20, 196, 440
SlaI CTCGAG 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 141, 935
SmlI CTYRAG 2 cut(s) 83, 815
SmoI CTYRAG 2 cut(s) 83, 815
Sse9I AATT 6 cut(s) 36, 353, 525, 639, 947, 980
SsiI CCGC 7 cut(s) 101, 206, 256, 338, 501, 750, 806
SspMI CTAG 2 cut(s) 557, 759
StyD4I CCNGG 4 cut(s) 146, 190, 214, 728
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 6 cut(s) 200, 234, 454, 592, 736, 866
TaiI ACGT 2 cut(s) 369, 774
TaqI TCGA 6 cut(s) 34, 602, 676, 705, 816, 840
TasI AATT 6 cut(s) 36, 353, 525, 639, 947, 980
TauI GCSGC 3 cut(s) 258, 503, 808
Tru1I TTAA 3 cut(s) 351, 642, 1056
Tru9I TTAA 3 cut(s) 351, 642, 1056
TscAI CASTG 2 cut(s) 248, 597
TseI GCWGC 3 cut(s) 225, 752, 755
TspDTI ATGAA 5 cut(s) 107, 293, 293, 600, 642
TspRI CASTG 2 cut(s) 248, 597
VneI GTGCAC 1 cut(s) 329
VpaK11BI GGWCC 3 cut(s) 20, 196, 440
XapI RAATTY 2 cut(s) 36, 980
XceI RCATGY 1 cut(s) 1063
XhoI CTCGAG 1 cut(s) 815
XmiI GTMKAC 2 cut(s) 156, 705
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 2 cut(s) 557, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.