MD01G1195500.v1.1

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001681
Physical Location & Seq
Forward (+)
29383738 .. 29385849
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1195500.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGGGAAGGGAGGCAAGTCGACTAACGGAGATGCCAAAGATGGCGAACAAGAAAACAAGGCTGCATGGCTTCTTGGTGTTAACAACCTCAAGATCCAGGCTTTCACTCTCCCGAGTCTTGGACCCAATGATGTCCGAATTAAGATCAAAGCCGTCGGCATTTGTGGAAGCGACGTCCATTACCTCAAGACCATGAAGTGCGGGGATTTTGAAGTTAAGGAGCCAATGGTGATTGGTCATGAGTGTGCTGGGATCGTAGACGCAGTCGGGAGCGCCGTGAAGCATCTGGTTTCTGGCGATCGCGTGGCATTAGAGCCCGGCATCAGCTGCGCAAAGTGTCACCAGTGCAAAGGAGGCCGCTACAATCTTTGCCCGGACATGAAGTTTTTCGCCACCCCGCCGGTTCATGGCTCCTTGGCGAACCAGATTGTGCATCCTGCGGACTTGTGCTTTAAGCTTCCGGAGAATGTTAGCTTGGAGGAAGGGGCAATGTGTGAGCCCTTGAGTGTTGGGGTTCATGCCTGTCGGAGAGCCAATGTCACTCCAGAAACAAATGTTCTCATCATCGGAGCAGGGCCGATCGGGCTGGTATCCGTGCTATCTGCTCGTGCTTTCGGGGCGCCGAGAATTGTCATTGTGGACATGGATGATCAGCGTCTAGCTATGGCAAAGTCTCTTGGTGCCGATGAAACTGTCAAAGTTTCAACAAAAGCCGAGGATTTGGATGACGAAGTTGCCAAGATTAAAAAAGCCATGAAATCCGAAGTGGATGTGACCTTTGATTGTGTAGGTTTCAACAAAACCATGGCGACAGCTCTCGCCGCGACTAGTCCCGGCGGCAAAGTCTGCCTCGTCGGAATGGGACACAGCATGATGACTGTCCCTCTCACTCCTGCTGCTGCCAGGGAGGTGGACGTGGTTGGAATTTTCCGATACAAGAACACATGGCCGCTTTGCATCGAGTTTCTAAGAAGCGGGAAGATTGACGTGAAACCGCTTATTACTCACCGTTTTGGATTTTCCCAGAAGGAAGTGGAAGATGCCTTTGAGACCAGTGCTCGTGGAGGCAATGCCATCAAAGTCATGTTTAATTTGTAA

Protein Analysis

366

Amino Acids

38.88

Weight (kDa)

7.51

Isoelectric Point (pI)

20.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 155 2e-28 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 162 - 291 2.1e-09 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 193 - 323 4.9e-29 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 226 - 356 1.5e-08 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
Acc16I TGCGCA 1 cut(s) 331
AccB1I GGYRCC 2 cut(s) 619, 680
AccI GTMKAC 2 cut(s) 20, 258
AccII CGCG 2 cut(s) 303, 824
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 9 cut(s) 201, 358, 398, 440, 822, 837, 950, 975, 995
AclWI GGATC 2 cut(s) 88, 260
AcoI YGGCCR 1 cut(s) 947
AcsI RAATTY 1 cut(s) 924
AcyI GRCGYC 2 cut(s) 174, 620
AfiI CCNNNNNNNGG 2 cut(s) 119, 407
AgsI TTSAA 3 cut(s) 212, 705, 796
AhlI ACTAGT 1 cut(s) 827
AjiI CACGTC 2 cut(s) 916, 988
AjnI CCWGG 2 cut(s) 96, 902
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AluBI AGCT 5 cut(s) 327, 457, 474, 662, 815
AluI AGCT 5 cut(s) 327, 457, 474, 662, 815
Alw21I GWGCWC 1 cut(s) 1060
Alw26I GTCTC 2 cut(s) 678, 1043
AlwI GGATC 2 cut(s) 88, 260
Ama87I CYCGRG 1 cut(s) 112
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 3 cut(s) 355, 575, 947
ApeKI GCWGC 4 cut(s) 62, 327, 896, 899
ApoI RAATTY 1 cut(s) 924
AsiSI GCGATCGC 1 cut(s) 301
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 3 cut(s) 275, 332, 622
AspS9I GGNCC 2 cut(s) 122, 575
AsuC2I CCSGG 3 cut(s) 318, 374, 834
AsuHPI GGTGA 3 cut(s) 241, 332, 998
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 122
BanI GGYRCC 2 cut(s) 619, 680
BanII GRGCYC 2 cut(s) 318, 501
BauI CACGAG 2 cut(s) 606, 1059
Bbv12I GWGCWC 1 cut(s) 1060
BbvI GCAGC 4 cut(s) 49, 314, 883, 886
BccI CCATC 2 cut(s) 35, 1082
BceAI ACGGC 2 cut(s) 137, 260
BciT130I CCWGG 2 cut(s) 98, 904
BciVI GTATCC 1 cut(s) 601
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 3 cut(s) 318, 374, 834
BcoDI GTCTC 2 cut(s) 678, 1043
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 2 cut(s) 659, 828
BfoI RGCGCY 2 cut(s) 276, 623
BfuI GTATCC 1 cut(s) 601
BglI GCCNNNNNGGC 1 cut(s) 583
BisI GCNGC 8 cut(s) 63, 328, 358, 822, 838, 897, 900, 950
BlsI GCNGC 8 cut(s) 64, 329, 359, 823, 839, 898, 901, 951
Bme1390I CCNGG 5 cut(s) 98, 318, 374, 834, 904
Bme18I GGWCC 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 2 cut(s) 916, 988
BmgT120I GGNCC 2 cut(s) 122, 575
BmiI GGNNCC 5 cut(s) 124, 222, 412, 621, 682
BmrFI CCNGG 5 cut(s) 98, 318, 374, 834, 904
BmsI GCATC 6 cut(s) 22, 292, 330, 442, 966, 1030
BpmI CTGGAG 1 cut(s) 528
BpuEI CTTGAG 3 cut(s) 74, 170, 523
BpuMI CCSGG 3 cut(s) 318, 374, 834
BsaHI GRCGYC 2 cut(s) 174, 620
BsaI GGTCTC 1 cut(s) 1043
BsaJI CCNNGG 4 cut(s) 414, 714, 804, 903
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 407
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 2 cut(s) 343, 1053
Bse3DI GCAATG 2 cut(s) 495, 1075
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 2 cut(s) 98, 904
BseDI CCNNGG 4 cut(s) 414, 714, 804, 903
BseGI GGATG 4 cut(s) 433, 652, 730, 775
BseLI CCNNNNNNNGG 2 cut(s) 119, 407
BseMI GCAATG 2 cut(s) 495, 1075
BseNI ACTGG 2 cut(s) 343, 1053
BseXI GCAGC 4 cut(s) 49, 314, 883, 886
BseYI CCCAGC 1 cut(s) 248
Bsh1236I CGCG 2 cut(s) 303, 824
Bsh1285I CGRYCG 2 cut(s) 301, 582
BshFI GGCC 3 cut(s) 357, 577, 949
BshNI GGYRCC 2 cut(s) 619, 680
BsiEI CGRYCG 2 cut(s) 301, 582
BsiHKAI GWGCWC 1 cut(s) 1060
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 5 cut(s) 318, 374, 401, 461, 834
BslFI GGGAC 3 cut(s) 816, 866, 876
BslI CCNNNNNNNGG 2 cut(s) 119, 407
BsmAI GTCTC 2 cut(s) 678, 1043
BsmFI GGGAC 3 cut(s) 816, 866, 876
BsnI GGCC 3 cut(s) 357, 577, 949
Bso31I GGTCTC 1 cut(s) 1043
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 3 cut(s) 318, 501, 1060
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 6 cut(s) 93, 144, 252, 298, 579, 649
Bsp19I CCATGG 1 cut(s) 804
BspACI CCGC 9 cut(s) 201, 358, 398, 440, 822, 837, 950, 975, 995
BspANI GGCC 3 cut(s) 357, 577, 949
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 2 cut(s) 303, 824
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 5 cut(s) 124, 222, 412, 621, 682
BspPI GGATC 2 cut(s) 88, 260
BspT107I GGYRCC 2 cut(s) 619, 680
BspTNI GGTCTC 1 cut(s) 1043
BsrDI GCAATG 2 cut(s) 495, 1075
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 2 cut(s) 343, 1053
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 4 cut(s) 414, 714, 804, 903
BssMI GATC 6 cut(s) 93, 144, 252, 298, 579, 649
BssNI GRCGYC 2 cut(s) 174, 620
BssSI CACGAG 2 cut(s) 606, 1059
BssT1I CCWWGG 2 cut(s) 414, 804
Bst2BI CACGAG 2 cut(s) 606, 1059
Bst2UI CCWGG 2 cut(s) 98, 904
Bst4CI ACNGT 3 cut(s) 694, 880, 1010
BstACI GRCGYC 2 cut(s) 174, 620
BstAPI GCANNNNNTGC 2 cut(s) 327, 846
BstDEI CTNAG 1 cut(s) 968
BstDSI CCRYGG 1 cut(s) 804
BstF5I GGATG 4 cut(s) 433, 652, 730, 775
BstFNI CGCG 2 cut(s) 303, 824
BstH2I RGCGCY 2 cut(s) 276, 623
BstHHI GCGC 3 cut(s) 275, 332, 622
BstKTI GATC 6 cut(s) 96, 147, 255, 301, 582, 652
BstMAI GTCTC 2 cut(s) 678, 1043
BstMBI GATC 6 cut(s) 93, 144, 252, 298, 579, 649
BstMCI CGRYCG 2 cut(s) 301, 582
BstMWI GCNNNNNNNGC 6 cut(s) 327, 354, 583, 617, 821, 846
BstNI CCWGG 2 cut(s) 98, 904
BstSCI CCNGG 5 cut(s) 96, 316, 372, 832, 902
BstUI CGCG 2 cut(s) 303, 824
BstV1I GCAGC 4 cut(s) 49, 314, 883, 886
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 910
BstYI RGATCY 1 cut(s) 93
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 3 cut(s) 357, 577, 949
BtgI CCRYGG 1 cut(s) 804
BtrI CACGTC 2 cut(s) 916, 988
BtsCI GGATG 4 cut(s) 433, 652, 730, 775
BtsIMutI CAGTG 2 cut(s) 350, 1060
CciI TCATGA 1 cut(s) 238
CfoI GCGC 3 cut(s) 275, 332, 622
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 122, 575
CseI GACGC 2 cut(s) 269, 644
DdeI CTNAG 1 cut(s) 968
DinI GGCGCC 1 cut(s) 621
DpnI GATC 6 cut(s) 95, 146, 254, 300, 581, 651
DpnII GATC 6 cut(s) 93, 144, 252, 298, 579, 649
EaeI YGGCCR 1 cut(s) 947
Eco130I CCWWGG 2 cut(s) 414, 804
Eco24I GRGCYC 2 cut(s) 318, 501
Eco31I GGTCTC 1 cut(s) 1043
Eco47I GGWCC 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 96, 902
EcoT14I CCWWGG 2 cut(s) 414, 804
EcoT38I GRGCYC 2 cut(s) 318, 501
EgeI GGCGCC 1 cut(s) 621
EheI GGCGCC 1 cut(s) 621
ErhI CCWWGG 2 cut(s) 414, 804
FaqI GGGAC 3 cut(s) 816, 866, 876
FauI CCCGC 3 cut(s) 194, 405, 968
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 2 cut(s) 20, 258
Fnu4HI GCNGC 8 cut(s) 63, 328, 358, 822, 838, 897, 900, 950
FokI GGATG 4 cut(s) 420, 659, 737, 782
FriOI GRGCYC 2 cut(s) 318, 501
Fsp4HI GCNGC 8 cut(s) 63, 328, 358, 822, 838, 897, 900, 950
FspBI CTAG 2 cut(s) 659, 828
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 3 cut(s) 274, 331, 621
GluI GCNGC 8 cut(s) 63, 328, 358, 822, 838, 897, 900, 950
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 2 cut(s) 276, 623
HaeIII GGCC 3 cut(s) 357, 577, 949
HapII CCGG 5 cut(s) 318, 374, 401, 461, 834
HgaI GACGC 2 cut(s) 269, 644
HhaI GCGC 3 cut(s) 275, 332, 622
Hin1I GRCGYC 2 cut(s) 174, 620
Hin6I GCGC 3 cut(s) 273, 330, 620
HinP1I GCGC 3 cut(s) 273, 330, 620
HincII GTYRAC 2 cut(s) 21, 82
HindII GTYRAC 2 cut(s) 21, 82
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 1 cut(s) 115
HpaI GTTAAC 1 cut(s) 82
HpaII CCGG 5 cut(s) 318, 374, 401, 461, 834
HphI GGTGA 3 cut(s) 241, 332, 998
Hpy166II GTNNAC 5 cut(s) 21, 82, 259, 640, 913
Hpy188I TCNGA 6 cut(s) 137, 528, 569, 763, 857, 932
Hpy188III TCNNGA 7 cut(s) 91, 112, 187, 239, 268, 461, 545
Hpy8I GTNNAC 5 cut(s) 21, 82, 259, 640, 913
Hpy99I CGWCG 3 cut(s) 158, 176, 857
HpyAV CCTTC 2 cut(s) 476, 1021
HpyCH4III ACNGT 3 cut(s) 694, 880, 1010
HpyCH4IV ACGT 3 cut(s) 174, 915, 987
HpyCH4V TGCA 4 cut(s) 65, 348, 433, 957
HpyF10VI GCNNNNNNNGC 6 cut(s) 327, 354, 583, 617, 821, 846
HpyF3I CTNAG 1 cut(s) 968
HpySE526I ACGT 3 cut(s) 174, 915, 987
Hsp92I GRCGYC 2 cut(s) 174, 620
HspAI GCGC 3 cut(s) 273, 330, 620
KasI GGCGCC 1 cut(s) 619
Kpn2I TCCGGA 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 649
KspAI GTTAAC 1 cut(s) 82
Kzo9I GATC 6 cut(s) 93, 144, 252, 298, 579, 649
LmnI GCTCC 4 cut(s) 220, 270, 416, 569
Lsp1109I GCAGC 4 cut(s) 49, 314, 883, 886
LweI GCATC 6 cut(s) 22, 292, 330, 442, 966, 1030
MaeI CTAG 2 cut(s) 659, 828
MaeII ACGT 3 cut(s) 174, 915, 987
MaeIII GTNAC 3 cut(s) 338, 538, 772
MalI GATC 6 cut(s) 95, 146, 254, 300, 581, 651
MboI GATC 6 cut(s) 93, 144, 252, 298, 579, 649
MboII GAAGA 2 cut(s) 991, 1049
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 3 cut(s) 318, 501, 1060
MluCI AATT 4 cut(s) 138, 627, 924, 1090
Mly113I GGCGCC 1 cut(s) 620
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 3 cut(s) 506, 835, 901
MroI TCCGGA 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 6 cut(s) 81, 141, 216, 453, 744, 1089
MslI CAYNNNNRTG 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 5 cut(s) 318, 374, 401, 461, 834
MspR9I CCNGG 5 cut(s) 98, 318, 374, 834, 904
MvaI CCWGG 2 cut(s) 98, 904
MvnI CGCG 2 cut(s) 303, 824
MwoI GCNNNNNNNGC 6 cut(s) 327, 354, 583, 617, 821, 846
NarI GGCGCC 1 cut(s) 620
NciI CCSGG 3 cut(s) 318, 374, 834
NcoI CCATGG 1 cut(s) 804
NdeII GATC 6 cut(s) 93, 144, 252, 298, 579, 649
NlaIV GGNNCC 5 cut(s) 124, 222, 412, 621, 682
NmeAIII GCCGAG 2 cut(s) 648, 739
NmuCI GTSAC 3 cut(s) 338, 538, 772
NsbI TGCGCA 1 cut(s) 331
PagI TCATGA 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 386
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 8 cut(s) 64, 329, 359, 823, 839, 898, 901, 951
Ple19I CGATCG 2 cut(s) 301, 582
PleI GAGTC 1 cut(s) 123
PluTI GGCGCC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 96, 902
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 2 cut(s) 96, 902
PspN4I GGNNCC 5 cut(s) 124, 222, 412, 621, 682
PspPI GGNCC 2 cut(s) 122, 575
PsuI RGATCY 1 cut(s) 93
PsyI GACNNNGTC 1 cut(s) 263
PvuI CGATCG 2 cut(s) 301, 582
PvuII CAGCTG 1 cut(s) 327
RgaI GCGATCGC 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 243
SalI GTCGAC 1 cut(s) 19
SaqAI TTAA 6 cut(s) 81, 141, 216, 453, 744, 1089
SatI GCNGC 8 cut(s) 63, 328, 358, 822, 838, 897, 900, 950
Sau3AI GATC 6 cut(s) 93, 144, 252, 298, 579, 649
Sau96I GGNCC 2 cut(s) 122, 575
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 5 cut(s) 98, 318, 374, 834, 904
SduI GDGCHC 3 cut(s) 318, 501, 1060
SfaAI GCGATCGC 1 cut(s) 301
SfaNI GCATC 6 cut(s) 22, 292, 330, 442, 966, 1030
SfoI GGCGCC 1 cut(s) 621
SgfI GCGATCGC 1 cut(s) 301
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 3 cut(s) 89, 185, 502
SmoI CTYRAG 3 cut(s) 89, 185, 502
SpeI ACTAGT 1 cut(s) 827
Sse9I AATT 4 cut(s) 138, 627, 924, 1090
SsiI CCGC 9 cut(s) 201, 358, 398, 440, 822, 837, 950, 975, 995
SspDI GGCGCC 1 cut(s) 619
SspMI CTAG 2 cut(s) 659, 828
StyD4I CCNGG 5 cut(s) 96, 316, 372, 832, 902
StyI CCWWGG 2 cut(s) 414, 804
TaaI ACNGT 3 cut(s) 694, 880, 1010
TaiI ACGT 3 cut(s) 177, 918, 990
TaqI TCGA 2 cut(s) 20, 960
TasI AATT 4 cut(s) 138, 627, 924, 1090
TauI GCSGC 4 cut(s) 360, 824, 840, 952
Tru1I TTAA 6 cut(s) 81, 141, 216, 453, 744, 1089
Tru9I TTAA 6 cut(s) 81, 141, 216, 453, 744, 1089
TscAI CASTG 2 cut(s) 350, 1060
TseFI GTSAC 3 cut(s) 338, 538, 772
TseI GCWGC 4 cut(s) 62, 327, 896, 899
Tsp45I GTSAC 3 cut(s) 338, 538, 772
TspDTI ATGAA 6 cut(s) 209, 395, 395, 506, 702, 770
TspGWI ACGGA 2 cut(s) 42, 583
TspRI CASTG 2 cut(s) 350, 1060
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 1 cut(s) 924
XmiI GTMKAC 2 cut(s) 20, 258
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 2 cut(s) 659, 828
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.