Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001681

Physical Location & Seq

Genomic Coordinates Forward (+)

29333895 .. 29335934

Gene Structure 2040 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1194600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGGTAAGGGAGGCAAAGCTGCTGAGCCAGAAAACATGGGTGCTTGGCTTCTTGGTGTTAACAACATCAAGATCCAACCTTTCAAGCTTCCCAGTGTTGGGGTCCATGATGTTCGAGTTCGGATCAAGGCTGTCGGCATTTGTGGAAGTGATGTTCACTACCTCAAGACCATGAAGTGTGCAGATTTTGAAGTTAAAGAGCCAATGGTAATTGGCCATGAGTGTGCTGGGATCGTTGAGGAAATCGGGGCCGAGGTGATGCATCTCGCTCTGGGCGATCGTGTGGCAGTAGAACCTGGAATCAGCTGCACACGGTGCCAGCAGTGCAAAGGTGGAAGGTACAATCTTTGCCCGGACATGAAGTTTTTTGCCACCCCTCCGGTTCATGGTTCCTTGGCAAATCAGATTGTGCATCCTGCGGATCTGTGCTTTAAGCTCCCGGAGAATGTGAGTTTGGAGGAAGGGGCAATGTGTGAGCCCTTGAGTGTTGGGGTTCATGCTTGCCGGAGAGCCAATGTGGGACCGGAATCAACTGTCCTGATCATCGGAGCCGGCCCCATCGGTCTGGTTTCGGTGCTGGCTGCCCGTGCATTTGGGTCACCCAGAATTGTCATTGTGGATATGGATGACCAGCGTCTAGCCATGGCGCAGTCTCTCGGTGCTAATGACACTGTCAAAGTTTCGACAAAAATGGAGGATTTGGATGATGAAATTGCCCAGATTAAGACAGCCATGAAATCCAACGTGGATGTGAGCTTCGATTGTGTCGGTTTTAACAAAACCATGTCAACAGCTCTTGGTGCCACCCGTCCCGGCGGCAAAGTTTGCCTTGTCGGAATGGGACACGGCACGATGACAGTCCCACTTACTCCGGCTGCTGCCAGGGAGGTTGATGTTGTTGGAATTTTCCGGTACAAGAACACATGGCCTCTTTGCCTCGAGTTTTTGAGAAGTGGGAAGATCGACGTGAAGCCGCTCATTACGCACCGCTTTGGGTTTAACCAAAAGGAGGTGGAAGAAGCATTTGAAACCAGTGCCCGTGGGGGAAATGCCATTAAGGTCATGTTTATTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003939 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0008150 GO:0008152 GO:0009506 GO:0016491 GO:0016614 GO:0016616 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00040 ko00051 ko01100 map00040 map00051 map01100

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

359

Amino Acids

38.38

Weight (kDa)

6.75

Isoelectric Point (pI)

29.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	36 - 148	1.7e-27	Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C	PF16912	155 - 284	1e-08	Glucose dehydrogenase C-terminus
ADH_zinc_N	PF00107	186 - 316	8.8e-28	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	233 - 349	1e-06	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51970 AT5G51970
fragaria_vesca	FvH4_7g28870 FvH4_7g28870
malus_domestica	MD00G1005600.v1.1 MD00G1005700.v1.1 MD01G1110100.v1.1 MD01G1194600.v1.1 MD01G1194700.v1.1 MD01G1195200.v1.1 MD01G1195300.v1.1 MD01G1195400.v1.1 MD01G1195500.v1.1 MD07G1261900.v1.1 MD07G1262100.v1.1 MD07G1262300.v1.1
prunus_persica	Prupe.2G288800_v2.0.a1 Prupe.2G288800_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.8G142900_v2.0.a1 Prupe.8G143000_v2.0.a1
pyrus_communis	pycom01g13720 pycom01g20540 pycom01g20550 pycom01g20560 pycom01g20580 pycom01g20600 pycom01g20610 pycom07g24070 pycom07g24080 pycom07g24090 pycom07g24100 pycom07g24130
rosa_chinensis	RchiOBHm_Chr1g0376551
rosa_laevigata	RLG00000026602
rosa_multiflora	Rmu_sc0001308.1_g000011 Rmu_sc0005594.1_g000014
rosa_roxburghii	Rroxscaffold_4G00282010 Rroxscaffold_4G00282130
rosa_rugosa	Rorug01G0396500
rosa_samantha	Rh1AG407600 Rh1AG412800 Rh1BG372300 Rh1CG385100 Rh1CG386100 Rh1DG402100 Rh1DG403200
rosa_wichuraiana	Rw1G036180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	315, 800
AccBSI	CCGCTC	1 cut(s)	976
AciI	CCGC	4 cut(s)	419, 816, 974, 988
AclWI	GGATC	4 cut(s)	67, 131, 239, 429
AcoI	YGGCCR	1 cut(s)	214
AcsI	RAATTY	1 cut(s)	903
AdeI	CACNNNGTG	1 cut(s)	315
AfaI	GTAC	2 cut(s)	341, 914
AfiI	CCNNNNNNNGG	4 cut(s)	98, 99, 386, 1009
AgsI	TTSAA	3 cut(s)	85, 191, 1028
AjiI	CACGTC	1 cut(s)	967
AjnI	CCWGG	2 cut(s)	295, 881
AjuI	GAANNNNNNNTTGG	2 cut(s)	437, 469
AloI	GAACNNNNNNTCC	2 cut(s)	138, 170
AluBI	AGCT	6 cut(s)	20, 88, 306, 436, 756, 794
AluI	AGCT	6 cut(s)	20, 88, 306, 436, 756, 794
Alw26I	GTCTC	1 cut(s)	657
AlwI	GGATC	4 cut(s)	67, 131, 239, 429
Ama87I	CYCGRG	1 cut(s)	938
AoxI	GGCC	4 cut(s)	214, 249, 553, 926
ApeKI	GCWGC	5 cut(s)	20, 306, 581, 875, 878
ApoI	RAATTY	1 cut(s)	903
AspLEI	GCGC	1 cut(s)	649
AspS9I	GGNCC	4 cut(s)	103, 249, 521, 554
AsuC2I	CCSGG	3 cut(s)	353, 440, 813
AsuHPI	GGTGA	2 cut(s)	268, 591
AvaI	CYCGRG	1 cut(s)	938
AvaII	GGWCC	2 cut(s)	103, 521
BaeGI	GKGCMC	1 cut(s)	1039
BalI	TGGCCA	1 cut(s)	216
BanI	GGYRCC	2 cut(s)	315, 800
BanII	GRGCYC	1 cut(s)	480
BbvI	GCAGC	5 cut(s)	7, 293, 568, 862, 865
BccI	CCATC	1 cut(s)	566
BceAI	ACGGC	1 cut(s)	862
BciT130I	CCWGG	2 cut(s)	297, 883
BclI	TGATCA	1 cut(s)	540
BcnI	CCSGG	3 cut(s)	353, 440, 813
BcoDI	GTCTC	1 cut(s)	657
BfaI	CTAG	1 cut(s)	638
BisI	GCNGC	7 cut(s)	21, 307, 582, 817, 876, 879, 974
BlpI	GCTNAGC	1 cut(s)	24
BlsI	GCNGC	7 cut(s)	22, 308, 583, 818, 877, 880, 975
Bme1390I	CCNGG	5 cut(s)	297, 353, 440, 813, 883
Bme18I	GGWCC	2 cut(s)	103, 521
BmeT110I	CYCGRG	1 cut(s)	938
BmgBI	CACGTC	1 cut(s)	967
BmgT120I	GGNCC	4 cut(s)	103, 249, 521, 554
BmiI	GGNNCC	8 cut(s)	104, 250, 317, 391, 522, 550, 556, 802
BmrFI	CCNGG	5 cut(s)	297, 353, 440, 813, 883
BmrI	ACTGGG	1 cut(s)	87
BmsI	GCATC	3 cut(s)	249, 271, 421
BmuI	ACTGGG	1 cut(s)	87
BoxI	GACNNNNGTC	1 cut(s)	633
Bpu1102I	GCTNAGC	1 cut(s)	24
BpuEI	CTTGAG	2 cut(s)	149, 502
BpuMI	CCSGG	3 cut(s)	353, 440, 813
BsaJI	CCNNGG	5 cut(s)	252, 393, 642, 882, 1039
BsaWI	WCCGGW	3 cut(s)	379, 523, 909
Bsc4I	CCNNNNNNNGG	4 cut(s)	98, 99, 386, 1009
Bse118I	RCCGGY	1 cut(s)	551
Bse1I	ACTGG	2 cut(s)	93, 1032
Bse3DI	GCAATG	1 cut(s)	474
BseBI	CCWGG	2 cut(s)	297, 883
BseDI	CCNNGG	5 cut(s)	252, 393, 642, 882, 1039
BseGI	GGATG	4 cut(s)	412, 631, 709, 754
BseLI	CCNNNNNNNGG	4 cut(s)	98, 99, 386, 1009
BseMI	GCAATG	1 cut(s)	474
BseMII	CTCAG	1 cut(s)	15
BseNI	ACTGG	2 cut(s)	93, 1032
BseSI	GKGCMC	1 cut(s)	1039
BseXI	GCAGC	5 cut(s)	7, 293, 568, 862, 865
BseYI	CCCAGC	1 cut(s)	227
BsgI	GTGCAG	2 cut(s)	201, 292
Bsh1285I	CGRYCG	1 cut(s)	280
BshFI	GGCC	4 cut(s)	216, 251, 555, 928
BshNI	GGYRCC	2 cut(s)	315, 800
BsiEI	CGRYCG	1 cut(s)	280
BsiHKCI	CYCGRG	1 cut(s)	938
BsiSI	CCGG	9 cut(s)	353, 380, 440, 505, 524, 552, 813, 872, 910
BslFI	GGGAC	4 cut(s)	534, 795, 845, 855
BslI	CCNNNNNNNGG	4 cut(s)	98, 99, 386, 1009
BsmAI	GTCTC	1 cut(s)	657
BsmFI	GGGAC	4 cut(s)	534, 795, 845, 855
BsnI	GGCC	4 cut(s)	216, 251, 555, 928
BsoBI	CYCGRG	1 cut(s)	938
Bsp1286I	GDGCHC	2 cut(s)	480, 1039
Bsp143I	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
Bsp1720I	GCTNAGC	1 cut(s)	24
Bsp19I	CCATGG	1 cut(s)	642
BspACI	CCGC	4 cut(s)	419, 816, 974, 988
BspANI	GGCC	4 cut(s)	216, 251, 555, 928
BspCNI	CTCAG	1 cut(s)	16
BspLI	GGNNCC	8 cut(s)	104, 250, 317, 391, 522, 550, 556, 802
BspPI	GGATC	4 cut(s)	67, 131, 239, 429
BspT107I	GGYRCC	2 cut(s)	315, 800
BsrBI	CCGCTC	1 cut(s)	976
BsrDI	GCAATG	1 cut(s)	474
BsrFI	RCCGGY	1 cut(s)	551
BsrI	ACTGG	2 cut(s)	93, 1032
BssAI	RCCGGY	1 cut(s)	551
BssECI	CCNNGG	5 cut(s)	252, 393, 642, 882, 1039
BssMI	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
BssT1I	CCWWGG	2 cut(s)	393, 642
Bst2UI	CCWGG	2 cut(s)	297, 883
Bst4CI	ACNGT	4 cut(s)	315, 535, 673, 859
BstAPI	GCANNNNNTGC	2 cut(s)	315, 825
BstC8I	GCNNGC	4 cut(s)	320, 502, 553, 579
BstDEI	CTNAG	1 cut(s)	24
BstDSI	CCRYGG	2 cut(s)	642, 1039
BstEII	GGTNACC	1 cut(s)	597
BstF5I	GGATG	4 cut(s)	412, 631, 709, 754
BstHHI	GCGC	1 cut(s)	649
BstKTI	GATC	7 cut(s)	75, 126, 234, 280, 424, 543, 963
BstMAI	GTCTC	1 cut(s)	657
BstMBI	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
BstMCI	CGRYCG	1 cut(s)	280
BstMWI	GCNNNNNNNGC	6 cut(s)	315, 324, 587, 800, 825, 982
BstNI	CCWGG	2 cut(s)	297, 883
BstPAI	GACNNNNGTC	1 cut(s)	633
BstPI	GGTNACC	1 cut(s)	597
BstSCI	CCNGG	5 cut(s)	295, 351, 438, 811, 881
BstSLI	GKGCMC	1 cut(s)	1039
BstV1I	GCAGC	5 cut(s)	7, 293, 568, 862, 865
BstX2I	RGATCY	2 cut(s)	72, 421
BstXI	CCANNNNNNTGG	1 cut(s)	565
BstYI	RGATCY	2 cut(s)	72, 421
BsuRI	GGCC	4 cut(s)	216, 251, 555, 928
BtgI	CCRYGG	2 cut(s)	642, 1039
BtrI	CACGTC	1 cut(s)	967
BtsCI	GGATG	4 cut(s)	412, 631, 709, 754
BtsI	GCAGTG	1 cut(s)	329
BtsIMutI	CAGTG	4 cut(s)	100, 329, 669, 1039
Cac8I	GCNNGC	4 cut(s)	320, 502, 553, 579
CfoI	GCGC	1 cut(s)	649
Cfr10I	RCCGGY	1 cut(s)	551
Cfr13I	GGNCC	4 cut(s)	103, 249, 521, 554
CseI	GACGC	1 cut(s)	623
Csp6I	GTAC	2 cut(s)	340, 913
CviQI	GTAC	2 cut(s)	340, 913
DdeI	CTNAG	1 cut(s)	24
DpnI	GATC	7 cut(s)	74, 125, 233, 279, 423, 542, 962
DpnII	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
DraIII	CACNNNGTG	1 cut(s)	315
EaeI	YGGCCR	1 cut(s)	214
Eco130I	CCWWGG	2 cut(s)	393, 642
Eco24I	GRGCYC	1 cut(s)	480
Eco47I	GGWCC	2 cut(s)	103, 521
Eco88I	CYCGRG	1 cut(s)	938
Eco91I	GGTNACC	1 cut(s)	597
EcoO65I	GGTNACC	1 cut(s)	597
EcoRII	CCWGG	2 cut(s)	295, 881
EcoT14I	CCWWGG	2 cut(s)	393, 642
EcoT22I	ATGCAT	1 cut(s)	264
EcoT38I	GRGCYC	1 cut(s)	480
ErhI	CCWWGG	2 cut(s)	393, 642
FalI	AAGNNNNNCTT	2 cut(s)	813, 845
FaqI	GGGAC	4 cut(s)	534, 795, 845, 855
FbaI	TGATCA	1 cut(s)	540
Fnu4HI	GCNGC	7 cut(s)	21, 307, 582, 817, 876, 879, 974
FokI	GGATG	4 cut(s)	399, 638, 716, 761
FriOI	GRGCYC	1 cut(s)	480
Fsp4HI	GCNGC	7 cut(s)	21, 307, 582, 817, 876, 879, 974
FspBI	CTAG	1 cut(s)	638
GlaI	GCGC	1 cut(s)	648
GluI	GCNGC	7 cut(s)	21, 307, 582, 817, 876, 879, 974
GsaI	CCCAGC	1 cut(s)	231
HaeIII	GGCC	4 cut(s)	216, 251, 555, 928
HapII	CCGG	9 cut(s)	353, 380, 440, 505, 524, 552, 813, 872, 910
HgaI	GACGC	1 cut(s)	623
HhaI	GCGC	1 cut(s)	649
Hin6I	GCGC	1 cut(s)	647
HinP1I	GCGC	1 cut(s)	647
HincII	GTYRAC	2 cut(s)	61, 789
HindII	GTYRAC	2 cut(s)	61, 789
HindIII	AAGCTT	1 cut(s)	86
HinfI	GANTC	2 cut(s)	300, 527
HpaI	GTTAAC	1 cut(s)	61
HpaII	CCGG	9 cut(s)	353, 380, 440, 505, 524, 552, 813, 872, 910
HphI	GGTGA	2 cut(s)	268, 591
Hpy166II	GTNNAC	3 cut(s)	61, 157, 789
Hpy188I	TCNGA	4 cut(s)	123, 405, 548, 836
Hpy188III	TCNNGA	3 cut(s)	70, 166, 538
Hpy8I	GTNNAC	3 cut(s)	61, 157, 789
Hpy99I	CGWCG	1 cut(s)	968
HpyAV	CCTTC	2 cut(s)	330, 455
HpyCH4III	ACNGT	4 cut(s)	315, 535, 673, 859
HpyCH4IV	ACGT	2 cut(s)	744, 966
HpyCH4V	TGCA	6 cut(s)	182, 262, 309, 327, 412, 590
HpyF10VI	GCNNNNNNNGC	6 cut(s)	315, 324, 587, 800, 825, 982
HpyF3I	CTNAG	1 cut(s)	24
HpySE526I	ACGT	2 cut(s)	744, 966
HspAI	GCGC	1 cut(s)	647
KroI	GCCGGC	1 cut(s)	551
KroNI	GCCGGC	1 cut(s)	553
Ksp22I	TGATCA	1 cut(s)	540
KspAI	GTTAAC	1 cut(s)	61
Kzo9I	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
LmnI	GCTCC	2 cut(s)	441, 548
Lsp1109I	GCAGC	5 cut(s)	7, 293, 568, 862, 865
LweI	GCATC	3 cut(s)	249, 271, 421
MaeI	CTAG	1 cut(s)	638
MaeII	ACGT	2 cut(s)	744, 966
MaeIII	GTNAC	1 cut(s)	597
MalI	GATC	7 cut(s)	74, 125, 233, 279, 423, 542, 962
MbiI	CCGCTC	1 cut(s)	976
MboI	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
MboII	GAAGA	2 cut(s)	970, 1028
MflI	RGATCY	2 cut(s)	72, 421
MhlI	GDGCHC	2 cut(s)	480, 1039
MlsI	TGGCCA	1 cut(s)	216
MluCI	AATT	4 cut(s)	210, 606, 711, 903
MluNI	TGGCCA	1 cut(s)	216
MmeI	TCCRAC	4 cut(s)	100, 765, 814, 880
Mox20I	TGGCCA	1 cut(s)	216
Mph1103I	ATGCAT	1 cut(s)	264
MroNI	GCCGGC	1 cut(s)	551
MscI	TGGCCA	1 cut(s)	216
MseI	TTAA	7 cut(s)	60, 195, 432, 723, 774, 999, 1056
MslI	CAYNNNNRTG	1 cut(s)	222
Msp20I	TGGCCA	1 cut(s)	216
MspA1I	CMGCKG	1 cut(s)	306
MspI	CCGG	9 cut(s)	353, 380, 440, 505, 524, 552, 813, 872, 910
MspR9I	CCNGG	5 cut(s)	297, 353, 440, 813, 883
MvaI	CCWGG	2 cut(s)	297, 883
MwoI	GCNNNNNNNGC	6 cut(s)	315, 324, 587, 800, 825, 982
NaeI	GCCGGC	1 cut(s)	553
NciI	CCSGG	3 cut(s)	353, 440, 813
NcoI	CCATGG	1 cut(s)	642
NdeII	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
NgoMIV	GCCGGC	1 cut(s)	551
NlaIV	GGNNCC	8 cut(s)	104, 250, 317, 391, 522, 550, 556, 802
NmeAIII	GCCGAG	1 cut(s)	277
NmuCI	GTSAC	1 cut(s)	597
NsiI	ATGCAT	1 cut(s)	264
PaeR7I	CTCGAG	1 cut(s)	938
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PdiI	GCCGGC	1 cut(s)	553
PfeI	GAWTC	2 cut(s)	300, 527
PflFI	GACNNNGTC	1 cut(s)	671
PfoI	TCCNGGA	1 cut(s)	438
PkrI	GCNGC	7 cut(s)	22, 308, 583, 818, 877, 880, 975
Ple19I	CGATCG	1 cut(s)	280
PshAI	GACNNNNGTC	1 cut(s)	633
Psp6I	CCWGG	2 cut(s)	295, 881
PspEI	GGTNACC	1 cut(s)	597
PspFI	CCCAGC	1 cut(s)	227
PspGI	CCWGG	2 cut(s)	295, 881
PspN4I	GGNNCC	8 cut(s)	104, 250, 317, 391, 522, 550, 556, 802
PspPI	GGNCC	4 cut(s)	103, 249, 521, 554
PspXI	VCTCGAGB	1 cut(s)	938
PsuI	RGATCY	2 cut(s)	72, 421
PsyI	GACNNNGTC	1 cut(s)	671
PvuI	CGATCG	1 cut(s)	280
PvuII	CAGCTG	1 cut(s)	306
RsaI	GTAC	2 cut(s)	341, 914
RsaNI	GTAC	2 cut(s)	340, 913
RseI	CAYNNNNRTG	1 cut(s)	222
SaqAI	TTAA	7 cut(s)	60, 195, 432, 723, 774, 999, 1056
SatI	GCNGC	7 cut(s)	21, 307, 582, 817, 876, 879, 974
Sau3AI	GATC	7 cut(s)	72, 123, 231, 277, 421, 540, 960
Sau96I	GGNCC	4 cut(s)	103, 249, 521, 554
ScrFI	CCNGG	5 cut(s)	297, 353, 440, 813, 883
SduI	GDGCHC	2 cut(s)	480, 1039
SfaNI	GCATC	3 cut(s)	249, 271, 421
Sfr274I	CTCGAG	1 cut(s)	938
SinI	GGWCC	2 cut(s)	103, 521
SlaI	CTCGAG	1 cut(s)	938
SmiMI	CAYNNNNRTG	1 cut(s)	222
SmlI	CTYRAG	3 cut(s)	164, 481, 938
SmoI	CTYRAG	3 cut(s)	164, 481, 938
Sse9I	AATT	4 cut(s)	210, 606, 711, 903
SsiI	CCGC	4 cut(s)	419, 816, 974, 988
SspMI	CTAG	1 cut(s)	638
StyD4I	CCNGG	5 cut(s)	295, 351, 438, 811, 881
StyI	CCWWGG	2 cut(s)	393, 642
TaaI	ACNGT	4 cut(s)	315, 535, 673, 859
TaiI	ACGT	2 cut(s)	747, 969
TaqI	TCGA	5 cut(s)	115, 683, 759, 939, 963
TaqII	GACCGA	1 cut(s)	551
TasI	AATT	4 cut(s)	210, 606, 711, 903
TauI	GCSGC	2 cut(s)	819, 976
TfiI	GAWTC	2 cut(s)	300, 527
Tru1I	TTAA	7 cut(s)	60, 195, 432, 723, 774, 999, 1056
Tru9I	TTAA	7 cut(s)	60, 195, 432, 723, 774, 999, 1056
TscAI	CASTG	4 cut(s)	100, 329, 676, 1039
TseFI	GTSAC	1 cut(s)	597
TseI	GCWGC	5 cut(s)	20, 306, 581, 875, 878
Tsp45I	GTSAC	1 cut(s)	597
TspDTI	ATGAA	6 cut(s)	188, 374, 374, 485, 723, 749
TspRI	CASTG	4 cut(s)	100, 329, 676, 1039
Tth111I	GACNNNGTC	1 cut(s)	671
VpaK11BI	GGWCC	2 cut(s)	103, 521
XapI	RAATTY	1 cut(s)	903
XcmI	CCANNNNNNNNNTGG	1 cut(s)	224
XhoI	CTCGAG	1 cut(s)	938
XspI	CTAG	1 cut(s)	638
Zsp2I	ATGCAT	1 cut(s)	264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.