pycom07g24130

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001681
Physical Location & Seq
Forward (+)
24905144 .. 24906804
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24130.4

Sequence Viewer

Length: 1053 bp
ATGGGGGAGGGAGGTAAGTCGGCTAACGGGGAGGCCAGAGAAGGGGGGGAACAAGAAAACAAGGCTGCATGGCTTATTGGTGTTAACAACCTCAAGATCCAGACTTTCAATCTCCCGAGACTTGGACCCAATGATGTTCGAATTAAGATCAAGGCCGTAGGCATTTGTGGAAGCGACGTTCATTACTTCAAGACCATGAAGTGTGGGGATTATGAAGTTAAGGAGCCAATGGTGATTGGCCATGAGTGTGCTGGGATCGTAGATGTAGTCGGAAGCGAGGTGAAGCATCTGGTTTCTGGCGATCGCGTGGCTTTAGAGCCTGGCATCAGCTGCGCGAAGTGTCATCAGTGCAAAGGAGGCCGCTACAATCTTTGCCCTGACATGAAGTTTTTCGCCACCCCGCCAGTTCATGGTTCCTTGGCGAACCAGATTGTGCATCCTGCGGACTTGTGCTTTAAGCTTCCCGAGAATGTGAGCTTGGAGGAAGGGGCAATGTGTGAGCCCTTGAGTGTTGGGGTTCATGCTTGTCGAAGAGCCAATGTCACTCCGGAAACAACTGTTCTGATCATCGGAGCAGGGCCGATTGGGCTTGTTTCCATGCTATCTGCTCGTGCTTTCGGGGCGCCAAGAATTGTCATTGTGGATATGGATGACCAGCGTCTAGCCATGGCAAAGTCTCTTGGTGCCGATGAAACCGTCAAAGTTTCAACAAAAGCTGAGGATTTGGATGCCGAAGTTGCCAAGATTAAAAAAGCCATGAAGTCCGAAGTGAATGTGACCTTTGACTGTGTAGGTTTCAGCAAAACCATGTCAACAGCTCTCGCCGCCACTGGTCCCGGCGGCAAGGTCTGCCTGGTCGGAATGGGACACAGCATGATGACTGTCCCTCTCACTCCCGCTACTGCCAGGGAGGTGGATGTGGTTGGAATTTTCCGATACAAGAATACCTGGCCGCTTTGCATCGAGTTTCTGAGAAGCGGGAAGATCGATCGTGAAGCCTCTTATTACGCACCGTTTCGGATTTTCCCAGAAGGAAGTGGAAGATGCCTTTGA

Protein Analysis

351

Amino Acids

37.46

Weight (kDa)

7.49

Isoelectric Point (pI)

24.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 622, 683
AccB7I CCANNNNNTGG 1 cut(s) 410
AccII CGCG 2 cut(s) 306, 335
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 8 cut(s) 361, 401, 443, 825, 840, 897, 953, 978
AclWI GGATC 2 cut(s) 91, 263
AcoI YGGCCR 2 cut(s) 238, 950
AcsI RAATTY 1 cut(s) 927
AcyI GRCGYC 1 cut(s) 623
AfiI CCNNNNNNNGG 3 cut(s) 42, 122, 410
AgsI TTSAA 3 cut(s) 109, 190, 708
AjnI CCWGG 4 cut(s) 319, 852, 905, 947
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 5 cut(s) 330, 460, 477, 716, 818
AluI AGCT 5 cut(s) 330, 460, 477, 716, 818
Alw26I GTCTC 2 cut(s) 112, 681
AlwI GGATC 2 cut(s) 91, 263
Ama87I CYCGRG 2 cut(s) 115, 464
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 6 cut(s) 33, 153, 238, 358, 578, 950
ApeKI GCWGC 2 cut(s) 65, 330
ApoI RAATTY 1 cut(s) 927
AsiSI GCGATCGC 1 cut(s) 304
Asp700I GAANNNNTTC 1 cut(s) 389
AspLEI GCGC 2 cut(s) 335, 625
AspS9I GGNCC 3 cut(s) 125, 578, 833
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 2 cut(s) 244, 292
AsuII TTCGAA 1 cut(s) 139
AvaI CYCGRG 2 cut(s) 115, 464
AvaII GGWCC 2 cut(s) 125, 833
BalI TGGCCA 1 cut(s) 240
BanI GGYRCC 2 cut(s) 622, 683
BanII GRGCYC 1 cut(s) 504
BauI CACGAG 1 cut(s) 609
BbvCI CCTCAGC 1 cut(s) 717
BbvI GCAGC 2 cut(s) 52, 317
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 4 cut(s) 321, 854, 907, 949
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 1 cut(s) 837
BcoDI GTCTC 2 cut(s) 112, 681
BfaI CTAG 1 cut(s) 662
BfoI RGCGCY 1 cut(s) 626
BglI GCCNNNNNGGC 1 cut(s) 586
BisI GCNGC 6 cut(s) 66, 331, 361, 825, 841, 953
BlsI GCNGC 6 cut(s) 67, 332, 362, 826, 842, 954
Bme1390I CCNGG 5 cut(s) 321, 837, 854, 907, 949
Bme18I GGWCC 2 cut(s) 125, 833
BmeT110I CYCGRG 2 cut(s) 115, 464
BmgT120I GGNCC 3 cut(s) 125, 578, 833
BmiI GGNNCC 6 cut(s) 127, 225, 415, 624, 685, 835
BmrFI CCNGG 5 cut(s) 321, 837, 854, 907, 949
BmsI GCATC 6 cut(s) 295, 333, 445, 718, 969, 1034
BoxI GACNNNNGTC 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 139
BpuEI CTTGAG 2 cut(s) 77, 526
BpuMI CCSGG 1 cut(s) 837
Bsa29I ATCGAT 1 cut(s) 987
BsaHI GRCGYC 1 cut(s) 623
BsaJI CCNNGG 3 cut(s) 417, 666, 906
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 122, 410
Bse1I ACTGG 2 cut(s) 404, 835
Bse3DI GCAATG 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 4 cut(s) 321, 854, 907, 949
BseCI ATCGAT 1 cut(s) 987
BseDI CCNNGG 3 cut(s) 417, 666, 906
BseGI GGATG 4 cut(s) 436, 655, 733, 922
BseLI CCNNNNNNNGG 3 cut(s) 42, 122, 410
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 2 cut(s) 708, 962
BseNI ACTGG 2 cut(s) 404, 835
BseXI GCAGC 2 cut(s) 52, 317
BseYI CCCAGC 1 cut(s) 251
Bsh1236I CGCG 2 cut(s) 306, 335
Bsh1285I CGRYCG 2 cut(s) 304, 991
BshFI GGCC 6 cut(s) 35, 155, 240, 360, 580, 952
BshNI GGYRCC 2 cut(s) 622, 683
BshVI ATCGAT 1 cut(s) 987
BsiEI CGRYCG 2 cut(s) 304, 991
BsiHKCI CYCGRG 2 cut(s) 115, 464
BsiSI CCGG 2 cut(s) 548, 837
BslFI GGGAC 3 cut(s) 819, 869, 879
BslI CCNNNNNNNGG 3 cut(s) 42, 122, 410
BsmAI GTCTC 2 cut(s) 112, 681
BsmFI GGGAC 3 cut(s) 819, 869, 879
BsnI GGCC 6 cut(s) 35, 155, 240, 360, 580, 952
BsoBI CYCGRG 2 cut(s) 115, 464
Bsp119I TTCGAA 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 504
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 8 cut(s) 361, 401, 443, 825, 840, 897, 953, 978
BspANI GGCC 6 cut(s) 35, 155, 240, 360, 580, 952
BspCNI CTCAG 2 cut(s) 709, 963
BspDI ATCGAT 1 cut(s) 987
BspEI TCCGGA 1 cut(s) 547
BspFNI CGCG 2 cut(s) 306, 335
BspLI GGNNCC 6 cut(s) 127, 225, 415, 624, 685, 835
BspPI GGATC 2 cut(s) 91, 263
BspQI GCTCTTC 1 cut(s) 526
BspT104I TTCGAA 1 cut(s) 139
BspT107I GGYRCC 2 cut(s) 622, 683
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 2 cut(s) 404, 835
BssECI CCNNGG 3 cut(s) 417, 666, 906
BssMI GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
BssNI GRCGYC 1 cut(s) 623
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 2 cut(s) 417, 666
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 4 cut(s) 321, 854, 907, 949
Bst4CI ACNGT 5 cut(s) 559, 697, 788, 883, 1014
Bst6I CTCTTC 1 cut(s) 526
BstACI GRCGYC 1 cut(s) 623
BstAPI GCANNNNNTGC 2 cut(s) 330, 849
BstBI TTCGAA 1 cut(s) 139
BstDEI CTNAG 2 cut(s) 717, 971
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 4 cut(s) 436, 655, 733, 922
BstFNI CGCG 2 cut(s) 306, 335
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 2 cut(s) 335, 625
BstKTI GATC 7 cut(s) 99, 150, 258, 304, 567, 987, 991
BstMAI GTCTC 2 cut(s) 112, 681
BstMBI GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
BstMCI CGRYCG 2 cut(s) 304, 991
BstMWI GCNNNNNNNGC 7 cut(s) 330, 357, 586, 620, 737, 824, 849
BstNI CCWGG 4 cut(s) 321, 854, 907, 949
BstPAI GACNNNNGTC 1 cut(s) 657
BstSCI CCNGG 5 cut(s) 319, 835, 852, 905, 947
BstUI CGCG 2 cut(s) 306, 335
BstV1I GCAGC 2 cut(s) 52, 317
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 913
BstYI RGATCY 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 987
BsuRI GGCC 6 cut(s) 35, 155, 240, 360, 580, 952
BsuTUI ATCGAT 1 cut(s) 987
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 4 cut(s) 436, 655, 733, 922
BtsIMutI CAGTG 2 cut(s) 353, 828
CfoI GCGC 2 cut(s) 335, 625
Cfr13I GGNCC 3 cut(s) 125, 578, 833
ClaI ATCGAT 1 cut(s) 987
CseI GACGC 1 cut(s) 647
DdeI CTNAG 2 cut(s) 717, 971
DinI GGCGCC 1 cut(s) 624
DpnI GATC 7 cut(s) 98, 149, 257, 303, 566, 986, 990
DpnII GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
EaeI YGGCCR 2 cut(s) 238, 950
Eam1104I CTCTTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 526
Eco130I CCWWGG 2 cut(s) 417, 666
Eco24I GRGCYC 1 cut(s) 504
Eco47I GGWCC 2 cut(s) 125, 833
Eco88I CYCGRG 2 cut(s) 115, 464
EcoRII CCWGG 4 cut(s) 319, 852, 905, 947
EcoT14I CCWWGG 2 cut(s) 417, 666
EcoT38I GRGCYC 1 cut(s) 504
EgeI GGCGCC 1 cut(s) 624
EheI GGCGCC 1 cut(s) 624
ErhI CCWWGG 2 cut(s) 417, 666
FaqI GGGAC 3 cut(s) 819, 869, 879
FauI CCCGC 3 cut(s) 408, 904, 971
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 6 cut(s) 66, 331, 361, 825, 841, 953
FokI GGATG 4 cut(s) 423, 662, 740, 929
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 6 cut(s) 66, 331, 361, 825, 841, 953
FspBI CTAG 1 cut(s) 662
GlaI GCGC 2 cut(s) 334, 624
GluI GCNGC 6 cut(s) 66, 331, 361, 825, 841, 953
GsaI CCCAGC 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 6 cut(s) 35, 155, 240, 360, 580, 952
HapII CCGG 2 cut(s) 548, 837
HgaI GACGC 1 cut(s) 647
HhaI GCGC 2 cut(s) 335, 625
Hin1I GRCGYC 1 cut(s) 623
Hin6I GCGC 2 cut(s) 333, 623
HinP1I GCGC 2 cut(s) 333, 623
HincII GTYRAC 2 cut(s) 85, 813
HindII GTYRAC 2 cut(s) 85, 813
HindIII AAGCTT 1 cut(s) 458
HpaI GTTAAC 1 cut(s) 85
HpaII CCGG 2 cut(s) 548, 837
HphI GGTGA 2 cut(s) 244, 292
Hpy166II GTNNAC 2 cut(s) 85, 813
Hpy188I TCNGA 8 cut(s) 272, 564, 572, 766, 860, 935, 972, 1020
Hpy188III TCNNGA 7 cut(s) 94, 100, 115, 190, 464, 548, 992
Hpy8I GTNNAC 2 cut(s) 85, 813
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 35, 479, 1025
HpyCH4III ACNGT 5 cut(s) 559, 697, 788, 883, 1014
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 4 cut(s) 68, 351, 436, 960
HpyF10VI GCNNNNNNNGC 7 cut(s) 330, 357, 586, 620, 737, 824, 849
HpyF3I CTNAG 2 cut(s) 717, 971
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 623
HspAI GCGC 2 cut(s) 333, 623
KasI GGCGCC 1 cut(s) 622
Kpn2I TCCGGA 1 cut(s) 547
Ksp22I TGATCA 1 cut(s) 564
KspAI GTTAAC 1 cut(s) 85
Kzo9I GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
LguI GCTCTTC 1 cut(s) 526
LmnI GCTCC 2 cut(s) 223, 572
Lsp1109I GCAGC 2 cut(s) 52, 317
LweI GCATC 6 cut(s) 295, 333, 445, 718, 969, 1034
MaeI CTAG 1 cut(s) 662
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 2 cut(s) 541, 775
MalI GATC 7 cut(s) 98, 149, 257, 303, 566, 986, 990
MboI GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
MboII GAAGA 3 cut(s) 543, 994, 1053
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 504
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 3 cut(s) 141, 630, 927
MluNI TGGCCA 1 cut(s) 240
Mly113I GGCGCC 1 cut(s) 623
MmeI TCCRAC 3 cut(s) 250, 838, 904
Mox20I TGGCCA 1 cut(s) 240
MroI TCCGGA 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 389
MscI TGGCCA 1 cut(s) 240
MseI TTAA 5 cut(s) 84, 144, 219, 456, 747
MslI CAYNNNNRTG 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 2 cut(s) 548, 837
MspR9I CCNGG 5 cut(s) 321, 837, 854, 907, 949
MvaI CCWGG 4 cut(s) 321, 854, 907, 949
MvnI CGCG 2 cut(s) 306, 335
MwoI GCNNNNNNNGC 7 cut(s) 330, 357, 586, 620, 737, 824, 849
NarI GGCGCC 1 cut(s) 623
NciI CCSGG 1 cut(s) 837
NcoI CCATGG 1 cut(s) 666
NdeII GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
NlaIV GGNNCC 6 cut(s) 127, 225, 415, 624, 685, 835
NmuCI GTSAC 2 cut(s) 541, 775
NspV TTCGAA 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 526
PdmI GAANNNNTTC 1 cut(s) 389
PflMI CCANNNNNTGG 1 cut(s) 410
PkrI GCNGC 6 cut(s) 67, 332, 362, 826, 842, 954
Ple19I CGATCG 2 cut(s) 304, 991
PluTI GGCGCC 1 cut(s) 626
PshAI GACNNNNGTC 1 cut(s) 657
Psp6I CCWGG 4 cut(s) 319, 852, 905, 947
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 4 cut(s) 319, 852, 905, 947
PspN4I GGNNCC 6 cut(s) 127, 225, 415, 624, 685, 835
PspPI GGNCC 3 cut(s) 125, 578, 833
PsuI RGATCY 1 cut(s) 96
PvuI CGATCG 2 cut(s) 304, 991
PvuII CAGCTG 1 cut(s) 330
RgaI GCGATCGC 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 526
SaqAI TTAA 5 cut(s) 84, 144, 219, 456, 747
SatI GCNGC 6 cut(s) 66, 331, 361, 825, 841, 953
Sau3AI GATC 7 cut(s) 96, 147, 255, 301, 564, 984, 988
Sau96I GGNCC 3 cut(s) 125, 578, 833
ScrFI CCNGG 5 cut(s) 321, 837, 854, 907, 949
SduI GDGCHC 1 cut(s) 504
SfaAI GCGATCGC 1 cut(s) 304
SfaNI GCATC 6 cut(s) 295, 333, 445, 718, 969, 1034
SfoI GGCGCC 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 139
SgfI GCGATCGC 1 cut(s) 304
SinI GGWCC 2 cut(s) 125, 833
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 2 cut(s) 92, 505
SmoI CTYRAG 2 cut(s) 92, 505
Sse9I AATT 3 cut(s) 141, 630, 927
SsiI CCGC 8 cut(s) 361, 401, 443, 825, 840, 897, 953, 978
SspDI GGCGCC 1 cut(s) 622
SspMI CTAG 1 cut(s) 662
StyD4I CCNGG 5 cut(s) 319, 835, 852, 905, 947
StyI CCWWGG 2 cut(s) 417, 666
TaaI ACNGT 5 cut(s) 559, 697, 788, 883, 1014
TaiI ACGT 1 cut(s) 180
TaqI TCGA 4 cut(s) 139, 529, 963, 987
TasI AATT 3 cut(s) 141, 630, 927
TauI GCSGC 4 cut(s) 363, 827, 843, 955
Tru1I TTAA 5 cut(s) 84, 144, 219, 456, 747
Tru9I TTAA 5 cut(s) 84, 144, 219, 456, 747
TscAI CASTG 2 cut(s) 353, 835
TseFI GTSAC 2 cut(s) 541, 775
TseI GCWGC 2 cut(s) 65, 330
Tsp45I GTSAC 2 cut(s) 541, 775
TspDTI ATGAA 8 cut(s) 170, 212, 228, 398, 398, 509, 705, 773
TspRI CASTG 2 cut(s) 353, 835
Van91I CCANNNNNTGG 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 125, 833
XapI RAATTY 1 cut(s) 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.