pycom01g13720

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001681
Physical Location & Seq
Reverse (-)
14114529 .. 14116737
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g13720.2

Sequence Viewer

Length: 1116 bp
ATGGGAAAGGGAGGCATGTCTGATGGAGATCATGCTGATCGCTGTTTTGGGGAAGCAATAAATGGTGATGTTCAACAAGAGAACATGGCTGCTTGGCTTCTTGGTGTTAAAAACCTCAAGATTCAACCTTACAAGCTCCCTAATCTTGGACCCCATGATGTTAGAGTCCGGCTGAAGGCTGTTGGCATATGTGGCAGTGATGTACACCACTTCAAGAACATGAGGTGTGTAGATTTTATAGTTAAAGAGCCAATGGTTATTGGGCATGAGTGTGCTGGGATCATAGAGGAAGTTGGGAGTGAGGTCGAAGATTTGGTGCCAGGCGATCGTGTGGCACTAGAGCCTGGTATCAGTTGCAAGCGATGCAACCTCTGCAAACAAGGCCGGTACAATCTATGCCGCAAGATGAAGTTTTTTGGCTCCCCTCCAAATAATGGTTGTCTGGCAAATCAGGTTGTCCATCCAGGAGATCTATGTTTTAAACTGCCAGACAATGTGAGTTTGGAGGAAGGCGCAATGTGTGAGCCTTTAAGTGTTGGTATTCATGCTTGTCGCCGGGCAAATGTCTGTCAAGACACAAATATCTTGGTTGTGGGAGCAGGACCTATAGGACTTGTTACACTGCTAGCCGCTCGTGCTTTTGGGGCGCCCCGAATTGTCATTGCGGATGTGAATGACGAGCGTTTGTCGATTGCAAAGAGTCTTGGCGCGGATGAAGTCGTTAAGGTTTCAACAAATATTGAGGATGTAGCTGAGGAAGTGGCTAAGATACAAAAGGTTTTGGAAAATGGAGTGGATATAAGCTTCGACTGTGCAGGCTTTAACAAAACCATAACAACAGCTTTGAGTGCTACTCGTCCCGGAGGCACAGTTTGCCTTGTGGGAATGGGTCAGAGAGAGATGACTCTCCCTCTCGCTACCAGAGAGATTGATATAATTGGAATTTTCCGATACCAGAACACATGGCCGCTGTGCCTTGAGTTTCTGAGAAGTGGTAAGATTGATGTGAAGCCCCTCATAACGCATCGGTTTGGATTTTCTCAGAAGGAGGTGGAAGAAGCCTTTGAAACCAGTGCTCGCGGAGGCAATGCCATTAAGGTCATGTTTAACCTGTAA

Protein Analysis

372

Amino Acids

40.23

Weight (kDa)

6.11

Isoelectric Point (pI)

27.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 316, 646
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 632
AccII CGCG 2 cut(s) 710, 1080
AciI CCGC 6 cut(s) 400, 630, 665, 710, 968, 1080
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 965
AcsI RAATTY 1 cut(s) 942
AcuI CTGAAG 1 cut(s) 194
AcyI GRCGYC 1 cut(s) 647
AfaI GTAC 2 cut(s) 204, 389
AfiI CCNNNNNNNGG 3 cut(s) 146, 175, 434
AgsI TTSAA 5 cut(s) 74, 125, 214, 732, 1067
AjnI CCWGG 3 cut(s) 319, 343, 463
AluBI AGCT 4 cut(s) 136, 752, 804, 842
AluI AGCT 4 cut(s) 136, 752, 804, 842
Alw21I GWGCWC 1 cut(s) 1078
AlwI GGATC 1 cut(s) 287
AoxI GGCC 2 cut(s) 382, 965
ApeKI GCWGC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 942
AspLEI GCGC 3 cut(s) 515, 649, 710
AspS9I GGNCC 2 cut(s) 149, 602
AsuC2I CCSGG 2 cut(s) 557, 861
AsuHPI GGTGA 1 cut(s) 77
AsuNHI GCTAGC 1 cut(s) 625
AvaII GGWCC 2 cut(s) 149, 602
BanI GGYRCC 2 cut(s) 316, 646
BauI CACGAG 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 1078
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 1 cut(s) 76
BccI CCATC 2 cut(s) 17, 468
BciT130I CCWGG 3 cut(s) 321, 345, 465
BcnI CCSGG 2 cut(s) 557, 861
BfaI CTAG 2 cut(s) 338, 626
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 650
BglII AGATCT 1 cut(s) 469
BisI GCNGC 4 cut(s) 90, 400, 630, 968
BlsI GCNGC 4 cut(s) 91, 401, 631, 969
Bme1390I CCNGG 5 cut(s) 321, 345, 465, 557, 861
Bme18I GGWCC 2 cut(s) 149, 602
BmgT120I GGNCC 2 cut(s) 149, 602
BmiI GGNNCC 4 cut(s) 151, 318, 421, 648
BmrFI CCNGG 5 cut(s) 321, 345, 465, 557, 861
BmsI GCATC 2 cut(s) 353, 1033
BmtI GCTAGC 1 cut(s) 629
BplI GAGNNNNNCTC 2 cut(s) 838, 870
Bpu10I CCTNAGC 1 cut(s) 753
BpuEI CTTGAG 2 cut(s) 101, 998
BpuMI CCSGG 2 cut(s) 557, 861
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 647
BsaXI ACNNNNNCTCC 2 cut(s) 855, 885
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 175, 434
Bse118I RCCGGY 1 cut(s) 384
Bse1I ACTGG 1 cut(s) 1071
Bse3DI GCAATG 3 cut(s) 522, 660, 1093
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 3 cut(s) 321, 345, 465
BseGI GGATG 4 cut(s) 460, 673, 718, 751
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 146, 175, 434
BseMI GCAATG 3 cut(s) 522, 660, 1093
BseMII CTCAG 3 cut(s) 744, 977, 1055
BseNI ACTGG 1 cut(s) 1071
BseXI GCAGC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 834
Bsh1236I CGCG 2 cut(s) 710, 1080
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 2 cut(s) 384, 967
BshNI GGYRCC 2 cut(s) 316, 646
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 1078
BsiSI CCGG 4 cut(s) 169, 385, 556, 861
BslFI GGGAC 1 cut(s) 843
BslI CCNNNNNNNGG 3 cut(s) 146, 175, 434
BsmFI GGGAC 1 cut(s) 843
BsnI GGCC 2 cut(s) 384, 967
Bsp1286I GDGCHC 1 cut(s) 1078
Bsp1407I TGTACA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 28, 37, 279, 325, 469
BspACI CCGC 6 cut(s) 400, 630, 665, 710, 968, 1080
BspANI GGCC 2 cut(s) 384, 967
BspCNI CTCAG 3 cut(s) 745, 978, 1054
BspFNI CGCG 2 cut(s) 710, 1080
BspLI GGNNCC 4 cut(s) 151, 318, 421, 648
BspOI GCTAGC 1 cut(s) 629
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 2 cut(s) 316, 646
BsrBI CCGCTC 1 cut(s) 632
BsrDI GCAATG 3 cut(s) 522, 660, 1093
BsrFI RCCGGY 1 cut(s) 384
BsrGI TGTACA 1 cut(s) 202
BsrI ACTGG 1 cut(s) 1071
BssAI RCCGGY 1 cut(s) 384
BssMI GATC 5 cut(s) 28, 37, 279, 325, 469
BssNI GRCGYC 1 cut(s) 647
BssSI CACGAG 1 cut(s) 633
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 3 cut(s) 321, 345, 465
Bst4CI ACNGT 2 cut(s) 812, 871
BstACI GRCGYC 1 cut(s) 647
BstAPI GCANNNNNTGC 3 cut(s) 363, 372, 873
BstAUI TGTACA 1 cut(s) 202
BstC8I GCNNGC 4 cut(s) 359, 627, 817, 1078
BstDEI CTNAG 4 cut(s) 753, 765, 986, 1041
BstF5I GGATG 4 cut(s) 460, 673, 718, 751
BstFNI CGCG 2 cut(s) 710, 1080
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 3 cut(s) 515, 649, 710
BstKTI GATC 5 cut(s) 31, 40, 282, 328, 472
BstMBI GATC 5 cut(s) 28, 37, 279, 325, 469
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 8 cut(s) 192, 363, 372, 381, 635, 644, 848, 873
BstNI CCWGG 3 cut(s) 321, 345, 465
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 5 cut(s) 319, 343, 463, 555, 859
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 2 cut(s) 710, 1080
BstV1I GCAGC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuRI GGCC 2 cut(s) 384, 967
BtgZI GCGATG 1 cut(s) 376
BtsCI GGATG 4 cut(s) 460, 673, 718, 751
BtsI GCAGTG 2 cut(s) 202, 620
BtsIMutI CAGTG 3 cut(s) 202, 620, 1078
Cac8I GCNNGC 4 cut(s) 359, 627, 817, 1078
CfoI GCGC 3 cut(s) 515, 649, 710
Cfr10I RCCGGY 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 149, 602
Csp6I GTAC 2 cut(s) 203, 388
CviAII CATG 9 cut(s) 16, 32, 85, 155, 220, 266, 545, 963, 1102
CviQI GTAC 2 cut(s) 203, 388
DdeI CTNAG 4 cut(s) 753, 765, 986, 1041
DinI GGCGCC 1 cut(s) 648
DpnI GATC 5 cut(s) 30, 39, 281, 327, 471
DpnII GATC 5 cut(s) 28, 37, 279, 325, 469
DraI TTTAAA 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 149, 602
Eco57I CTGAAG 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 3 cut(s) 319, 343, 463
EgeI GGCGCC 1 cut(s) 648
EheI GGCGCC 1 cut(s) 648
FaeI CATG 9 cut(s) 19, 35, 88, 158, 223, 269, 548, 966, 1105
FaqI GGGAC 1 cut(s) 843
FatI CATG 9 cut(s) 15, 31, 84, 154, 219, 265, 544, 962, 1101
FauNDI CATATG 1 cut(s) 188
Fnu4HI GCNGC 4 cut(s) 90, 400, 630, 968
FokI GGATG 4 cut(s) 447, 680, 725, 758
Fsp4HI GCNGC 4 cut(s) 90, 400, 630, 968
FspBI CTAG 2 cut(s) 338, 626
GlaI GCGC 3 cut(s) 514, 648, 709
GluI GCNGC 4 cut(s) 90, 400, 630, 968
GsaI CCCAGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 2 cut(s) 384, 967
HapII CCGG 4 cut(s) 169, 385, 556, 861
HhaI GCGC 3 cut(s) 515, 649, 710
Hin1I GRCGYC 1 cut(s) 647
Hin1II CATG 9 cut(s) 19, 35, 88, 158, 223, 269, 548, 966, 1105
Hin6I GCGC 3 cut(s) 513, 647, 708
HinP1I GCGC 3 cut(s) 513, 647, 708
HindIII AAGCTT 1 cut(s) 802
HinfI GANTC 4 cut(s) 121, 165, 700, 904
HpaII CCGG 4 cut(s) 169, 385, 556, 861
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 5 cut(s) 22, 894, 950, 987, 1044
Hpy188III TCNNGA 3 cut(s) 118, 214, 572
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 3 cut(s) 169, 503, 1039
HpyCH4III ACNGT 2 cut(s) 812, 871
HpyCH4V TGCA 5 cut(s) 357, 366, 375, 695, 815
HpyF10VI GCNNNNNNNGC 8 cut(s) 192, 363, 372, 381, 635, 644, 848, 873
HpyF3I CTNAG 4 cut(s) 753, 765, 986, 1041
Hsp92I GRCGYC 1 cut(s) 647
Hsp92II CATG 9 cut(s) 19, 35, 88, 158, 223, 269, 548, 966, 1105
HspAI GCGC 3 cut(s) 513, 647, 708
KasI GGCGCC 1 cut(s) 646
Kzo9I GATC 5 cut(s) 28, 37, 279, 325, 469
LmnI GCTCC 3 cut(s) 141, 425, 596
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 2 cut(s) 353, 1033
MaeI CTAG 2 cut(s) 338, 626
MaeIII GTNAC 1 cut(s) 616
MalI GATC 5 cut(s) 30, 39, 281, 327, 471
MbiI CCGCTC 1 cut(s) 632
MboI GATC 5 cut(s) 28, 37, 279, 325, 469
MboII GAAGA 2 cut(s) 320, 1067
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 1078
MluCI AATT 3 cut(s) 654, 936, 942
Mly113I GGCGCC 1 cut(s) 647
MlyI GAGTC 3 cut(s) 174, 709, 898
MseI TTAA 8 cut(s) 108, 243, 480, 530, 723, 822, 1095, 1107
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 970
MspI CCGG 4 cut(s) 169, 385, 556, 861
MspR9I CCNGG 5 cut(s) 321, 345, 465, 557, 861
MvaI CCWGG 3 cut(s) 321, 345, 465
MvnI CGCG 2 cut(s) 710, 1080
MwoI GCNNNNNNNGC 8 cut(s) 192, 363, 372, 381, 635, 644, 848, 873
NarI GGCGCC 1 cut(s) 647
NciI CCSGG 2 cut(s) 557, 861
NdeI CATATG 1 cut(s) 188
NdeII GATC 5 cut(s) 28, 37, 279, 325, 469
NheI GCTAGC 1 cut(s) 625
NlaIII CATG 9 cut(s) 19, 35, 88, 158, 223, 269, 548, 966, 1105
NlaIV GGNNCC 4 cut(s) 151, 318, 421, 648
NspI RCATGY 1 cut(s) 19
PfeI GAWTC 1 cut(s) 121
PflMI CCANNNNNTGG 1 cut(s) 434
PfoI TCCNGGA 2 cut(s) 463, 859
PkrI GCNGC 4 cut(s) 91, 401, 631, 969
Ple19I CGATCG 1 cut(s) 328
PleI GAGTC 3 cut(s) 173, 708, 898
PluTI GGCGCC 1 cut(s) 650
PpsI GAGTC 3 cut(s) 173, 708, 898
PpuMI RGGWCCY 1 cut(s) 602
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 319, 343, 463
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 3 cut(s) 319, 343, 463
PspN4I GGNNCC 4 cut(s) 151, 318, 421, 648
PspPI GGNCC 2 cut(s) 149, 602
PspPPI RGGWCCY 1 cut(s) 602
PsuI RGATCY 1 cut(s) 469
PvuI CGATCG 1 cut(s) 328
RsaI GTAC 2 cut(s) 204, 389
RsaNI GTAC 2 cut(s) 203, 388
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 8 cut(s) 108, 243, 480, 530, 723, 822, 1095, 1107
SatI GCNGC 4 cut(s) 90, 400, 630, 968
Sau3AI GATC 5 cut(s) 28, 37, 279, 325, 469
Sau96I GGNCC 2 cut(s) 149, 602
SchI GAGTC 3 cut(s) 174, 709, 898
ScrFI CCNGG 5 cut(s) 321, 345, 465, 557, 861
SduI GDGCHC 1 cut(s) 1078
SfaNI GCATC 2 cut(s) 353, 1033
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 648
SinI GGWCC 2 cut(s) 149, 602
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 2 cut(s) 116, 977
SmoI CTYRAG 2 cut(s) 116, 977
Sse9I AATT 3 cut(s) 654, 936, 942
SsiI CCGC 6 cut(s) 400, 630, 665, 710, 968, 1080
SspDI GGCGCC 1 cut(s) 646
SspI AATATT 1 cut(s) 739
SspMI CTAG 2 cut(s) 338, 626
StyD4I CCNGG 5 cut(s) 319, 343, 463, 555, 859
TaaI ACNGT 2 cut(s) 812, 871
TaqI TCGA 3 cut(s) 306, 689, 807
TasI AATT 3 cut(s) 654, 936, 942
TatI WGTACW 1 cut(s) 202
TauI GCSGC 3 cut(s) 402, 632, 970
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 8 cut(s) 108, 243, 480, 530, 723, 822, 1095, 1107
Tru9I TTAA 8 cut(s) 108, 243, 480, 530, 723, 822, 1095, 1107
TscAI CASTG 3 cut(s) 202, 627, 1078
TseI GCWGC 1 cut(s) 89
TspDTI ATGAA 3 cut(s) 422, 533, 729
TspRI CASTG 3 cut(s) 202, 627, 1078
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 149, 602
XapI RAATTY 1 cut(s) 942
XceI RCATGY 1 cut(s) 19
XspI CTAG 2 cut(s) 338, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.