Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001681

Physical Location & Seq

Genomic Coordinates Forward (+)

29367235 .. 29368638

Gene Structure 1404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1195300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGGTAAGGGTGGGCAAAAGATTTATGGATTGGCTAAGAAAGCCAAACCTGTTCAGCAAGAAAACATGGCTGCTTATCTTGTTGATGTCAACACCATCAAGATCCTACCTTTCAAGCTCCCCAGTATCGGACCCAATGATGTCCGGATTCGGATTAAGGCGGTTGGTATTTGTGGAAGTGATGTTCACTACCTCAAGACCATGAAATGTGCGGATTTTGAGGTTAAAGAGCCGATGGTGATGGGACATGAGTGTGCTGGGATCGTAGACAAAGTTGGGAGCGAGGTGAAGCATCTGGTGCCTGGTGACCGGGTGGCGGTTGAGCCCGGTATCAGTTGCTCACGGTGTCAGCAGTGCAAAGGAGGGCAGTACAATCTTTGCCCCGACATGAAGTTTTTCGCCACCCCACCGGTTCATGGTTCCTTGGCAAATCAGATTGTGCATCCTGCGGATCTATGCTTCAAGCTGCCAGAGAATGTGAGCTTGGAGGAAGGGGCAATGTGCGAGCCCTTGAGTGTTGGAGTTCATGCATGTCGGCGAGCCAATGTTGGTCCCGAAACAACTGTTCTGATCATCGGAGCAGGGCCTATTGGTCTGGTTTCAGTTTTGGCCGCTCGTGCTTTCGGGGCACCAAGAATTGTCATTGTGGATATGGACGACAAGCGTTTAGCCATGGCAATGTCTCTCGGCGCTGATGGCACCGTCAAAGTTTCAACAAAAATGGAGGATTTGGATGACGAAGTTGCCAAGATTAAAGAAGCAATGGAATCCGAGGTGGATGTGACCTTTGATTGTGTGGGTTTTAATAAAACCATGTCGACGGGTCTCAACGCCACTCGCCCCGGCGGCAAAGTCTGCCTTGTCGGAATGGGACACGGCATGATGACAGTGCCTCTCACTCCAGCTGCTGCCAGGGAGGTTGATGTGGTTGGTGTTTTCCGGTACAAGAACACATGGCCACTTTGCCTCGAGTTTTTGAGAAGTGGGAAGATCGACGTGAAGCCGCTTATTACTCACCGGTTTGGATTTACCGAGAAGGAGGTGGAAGAAGCCTTTGCTACCAGTGCTCGTGGGGGTGACGCCATCAAAGTCATGTTTAATCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003939 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0008150 GO:0008152 GO:0009506 GO:0016491 GO:0016614 GO:0016616 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00040 ko00051 ko01100 map00040 map00051 map01100

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

369

Amino Acids

39.52

Weight (kDa)

7.5

Isoelectric Point (pI)

25.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	44 - 158	5.9e-29	Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C	PF16912	165 - 294	7.5e-09	Glucose dehydrogenase C-terminus
ADH_zinc_N	PF00107	196 - 326	1.6e-28	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	230 - 358	4.5e-07	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51970 AT5G51970
fragaria_vesca	FvH4_7g28870 FvH4_7g28870
malus_domestica	MD00G1005600.v1.1 MD00G1005700.v1.1 MD01G1110100.v1.1 MD01G1194600.v1.1 MD01G1194700.v1.1 MD01G1195200.v1.1 MD01G1195300.v1.1 MD01G1195400.v1.1 MD01G1195500.v1.1 MD07G1261900.v1.1 MD07G1262100.v1.1 MD07G1262300.v1.1
prunus_persica	Prupe.2G288800_v2.0.a1 Prupe.2G288800_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.8G142900_v2.0.a1 Prupe.8G143000_v2.0.a1
pyrus_communis	pycom01g13720 pycom01g20540 pycom01g20550 pycom01g20560 pycom01g20580 pycom01g20600 pycom01g20610 pycom07g24070 pycom07g24080 pycom07g24090 pycom07g24100 pycom07g24130
rosa_chinensis	RchiOBHm_Chr1g0376551
rosa_laevigata	RLG00000026602
rosa_multiflora	Rmu_sc0001308.1_g000011 Rmu_sc0005594.1_g000014
rosa_roxburghii	Rroxscaffold_4G00282010 Rroxscaffold_4G00282130
rosa_rugosa	Rorug01G0396500
rosa_samantha	Rh1AG407600 Rh1AG412800 Rh1BG372300 Rh1CG385100 Rh1CG386100 Rh1DG402100 Rh1DG403200
rosa_wichuraiana	Rw1G036180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	298, 628, 698
AccBSI	CCGCTC	1 cut(s)	614
AccI	GTMKAC	2 cut(s)	267, 818
AccIII	TCCGGA	1 cut(s)	144
AciI	CCGC	7 cut(s)	161, 212, 317, 449, 612, 846, 1004
AclWI	GGATC	3 cut(s)	97, 269, 459
AcoI	YGGCCR	2 cut(s)	609, 956
AcyI	GRCGYC	1 cut(s)	1080
AfaI	GTAC	2 cut(s)	371, 944
AfiI	CCNNNNNNNGG	3 cut(s)	128, 316, 416
AgeI	ACCGGT	2 cut(s)	409, 1017
AgsI	TTSAA	3 cut(s)	115, 463, 714
AjiI	CACGTC	1 cut(s)	997
AjnI	CCWGG	2 cut(s)	301, 911
AjuI	GAANNNNNNNTTGG	2 cut(s)	467, 499
AloI	GAACNNNNNNTCC	2 cut(s)	168, 200
AluBI	AGCT	4 cut(s)	118, 466, 483, 905
AluI	AGCT	4 cut(s)	118, 466, 483, 905
Alw21I	GWGCWC	1 cut(s)	1069
Alw26I	GTCTC	2 cut(s)	687, 830
AlwI	GGATC	3 cut(s)	97, 269, 459
AlwNI	CAGNNNCTG	1 cut(s)	908
Ama87I	CYCGRG	1 cut(s)	968
Aor13HI	TCCGGA	1 cut(s)	144
AoxI	GGCC	3 cut(s)	584, 609, 956
ApeKI	GCWGC	4 cut(s)	71, 466, 905, 908
AsiGI	ACCGGT	2 cut(s)	409, 1017
Asp700I	GAANNNNTTC	1 cut(s)	395
AspLEI	GCGC	1 cut(s)	692
AspS9I	GGNCC	3 cut(s)	131, 551, 584
AsuC2I	CCSGG	3 cut(s)	311, 327, 843
AsuHPI	GGTGA	5 cut(s)	250, 298, 317, 1007, 1088
AvaI	CYCGRG	1 cut(s)	968
AvaII	GGWCC	2 cut(s)	131, 551
BaeGI	GKGCMC	1 cut(s)	631
BalI	TGGCCA	1 cut(s)	958
BanI	GGYRCC	3 cut(s)	298, 628, 698
BanII	GRGCYC	2 cut(s)	327, 510
BauI	CACGAG	2 cut(s)	615, 1068
Bbv12I	GWGCWC	1 cut(s)	1069
BbvI	GCAGC	4 cut(s)	58, 453, 892, 895
BccI	CCATC	5 cut(s)	104, 229, 235, 689, 1091
BceAI	ACGGC	1 cut(s)	892
BciT130I	CCWGG	2 cut(s)	303, 913
BclI	TGATCA	1 cut(s)	570
BcnI	CCSGG	3 cut(s)	311, 327, 843
BcoDI	GTCTC	2 cut(s)	687, 830
BfmI	CTRYAG	1 cut(s)	1103
BfoI	RGCGCY	1 cut(s)	693
BglI	GCCNNNNNGGC	1 cut(s)	846
BisI	GCNGC	7 cut(s)	72, 467, 612, 847, 906, 909, 1004
BlsI	GCNGC	7 cut(s)	73, 468, 613, 848, 907, 910, 1005
Bme1390I	CCNGG	5 cut(s)	303, 311, 327, 843, 913
Bme18I	GGWCC	2 cut(s)	131, 551
BmeT110I	CYCGRG	1 cut(s)	968
BmgBI	CACGTC	1 cut(s)	997
BmgT120I	GGNCC	3 cut(s)	131, 551, 584
BmiI	GGNNCC	6 cut(s)	133, 300, 421, 553, 630, 700
BmrFI	CCNGG	5 cut(s)	303, 311, 327, 843, 913
BmrI	ACTGGG	1 cut(s)	117
BmsI	GCATC	2 cut(s)	301, 451
BmuI	ACTGGG	1 cut(s)	117
BpmI	CTGGAG	1 cut(s)	885
BpuEI	CTTGAG	2 cut(s)	179, 532
BpuMI	CCSGG	3 cut(s)	311, 327, 843
BsaHI	GRCGYC	1 cut(s)	1080
BsaI	GGTCTC	1 cut(s)	830
BsaJI	CCNNGG	5 cut(s)	423, 672, 771, 841, 912
BsaWI	WCCGGW	4 cut(s)	144, 409, 939, 1017
Bsc4I	CCNNNNNNNGG	3 cut(s)	128, 316, 416
Bse118I	RCCGGY	2 cut(s)	409, 1017
Bse1I	ACTGG	2 cut(s)	123, 1062
Bse3DI	GCAATG	3 cut(s)	504, 684, 768
BseAI	TCCGGA	1 cut(s)	144
BseBI	CCWGG	2 cut(s)	303, 913
BseDI	CCNNGG	5 cut(s)	423, 672, 771, 841, 912
BseGI	GGATG	3 cut(s)	442, 739, 784
BseLI	CCNNNNNNNGG	3 cut(s)	128, 316, 416
BseMI	GCAATG	3 cut(s)	504, 684, 768
BseNI	ACTGG	2 cut(s)	123, 1062
BseSI	GKGCMC	1 cut(s)	631
BseXI	GCAGC	4 cut(s)	58, 453, 892, 895
BseYI	CCCAGC	1 cut(s)	257
BshFI	GGCC	3 cut(s)	586, 611, 958
BshNI	GGYRCC	3 cut(s)	298, 628, 698
BshTI	ACCGGT	2 cut(s)	409, 1017
BsiHKAI	GWGCWC	1 cut(s)	1069
BsiHKCI	CYCGRG	1 cut(s)	968
BsiSI	CCGG	7 cut(s)	145, 310, 327, 410, 843, 940, 1018
BslFI	GGGAC	3 cut(s)	258, 537, 885
BslI	CCNNNNNNNGG	3 cut(s)	128, 316, 416
BsmAI	GTCTC	2 cut(s)	687, 830
BsmFI	GGGAC	3 cut(s)	258, 537, 885
BsnI	GGCC	3 cut(s)	586, 611, 958
Bso31I	GGTCTC	1 cut(s)	830
BsoBI	CYCGRG	1 cut(s)	968
Bsp1286I	GDGCHC	4 cut(s)	327, 510, 631, 1069
Bsp13I	TCCGGA	1 cut(s)	144
Bsp143I	GATC	5 cut(s)	102, 261, 451, 570, 990
Bsp19I	CCATGG	1 cut(s)	672
BspACI	CCGC	7 cut(s)	161, 212, 317, 449, 612, 846, 1004
BspANI	GGCC	3 cut(s)	586, 611, 958
BspEI	TCCGGA	1 cut(s)	144
BspLI	GGNNCC	6 cut(s)	133, 300, 421, 553, 630, 700
BspPI	GGATC	3 cut(s)	97, 269, 459
BspT107I	GGYRCC	3 cut(s)	298, 628, 698
BspTNI	GGTCTC	1 cut(s)	830
BsrBI	CCGCTC	1 cut(s)	614
BsrDI	GCAATG	3 cut(s)	504, 684, 768
BsrFI	RCCGGY	2 cut(s)	409, 1017
BsrI	ACTGG	2 cut(s)	123, 1062
BssAI	RCCGGY	2 cut(s)	409, 1017
BssECI	CCNNGG	5 cut(s)	423, 672, 771, 841, 912
BssMI	GATC	5 cut(s)	102, 261, 451, 570, 990
BssNI	GRCGYC	1 cut(s)	1080
BssSI	CACGAG	2 cut(s)	615, 1068
BssT1I	CCWWGG	2 cut(s)	423, 672
Bst2BI	CACGAG	2 cut(s)	615, 1068
Bst2UI	CCWGG	2 cut(s)	303, 913
Bst4CI	ACNGT	4 cut(s)	345, 565, 703, 889
BstACI	GRCGYC	1 cut(s)	1080
BstAPI	GCANNNNNTGC	2 cut(s)	298, 855
BstC8I	GCNNGC	2 cut(s)	506, 540
BstDEI	CTNAG	1 cut(s)	36
BstDSI	CCRYGG	1 cut(s)	672
BstEII	GGTNACC	1 cut(s)	305
BstF5I	GGATG	3 cut(s)	442, 739, 784
BstH2I	RGCGCY	1 cut(s)	693
BstHHI	GCGC	1 cut(s)	692
BstKTI	GATC	5 cut(s)	105, 264, 454, 573, 993
BstMAI	GTCTC	2 cut(s)	687, 830
BstMBI	GATC	5 cut(s)	102, 261, 451, 570, 990
BstMWI	GCNNNNNNNGC	8 cut(s)	41, 298, 617, 626, 696, 846, 855, 1064
BstNI	CCWGG	2 cut(s)	303, 913
BstNSI	RCATGY	1 cut(s)	534
BstPI	GGTNACC	1 cut(s)	305
BstSCI	CCNGG	5 cut(s)	301, 309, 325, 841, 911
BstSFI	CTRYAG	1 cut(s)	1103
BstSLI	GKGCMC	1 cut(s)	631
BstV1I	GCAGC	4 cut(s)	58, 453, 892, 895
BstX2I	RGATCY	2 cut(s)	102, 451
BstYI	RGATCY	2 cut(s)	102, 451
BsuRI	GGCC	3 cut(s)	586, 611, 958
BtgI	CCRYGG	1 cut(s)	672
BtrI	CACGTC	1 cut(s)	997
BtsCI	GGATG	3 cut(s)	442, 739, 784
BtsI	GCAGTG	1 cut(s)	359
BtsIMutI	CAGTG	3 cut(s)	359, 894, 1069
Cac8I	GCNNGC	2 cut(s)	506, 540
CaiI	CAGNNNCTG	1 cut(s)	908
CfoI	GCGC	1 cut(s)	692
Cfr10I	RCCGGY	2 cut(s)	409, 1017
Cfr13I	GGNCC	3 cut(s)	131, 551, 584
CseI	GACGC	1 cut(s)	1088
Csp6I	GTAC	2 cut(s)	370, 943
CspAI	ACCGGT	2 cut(s)	409, 1017
CviQI	GTAC	2 cut(s)	370, 943
DdeI	CTNAG	1 cut(s)	36
DpnI	GATC	5 cut(s)	104, 263, 453, 572, 992
DpnII	GATC	5 cut(s)	102, 261, 451, 570, 990
EaeI	YGGCCR	2 cut(s)	609, 956
Eco130I	CCWWGG	2 cut(s)	423, 672
Eco24I	GRGCYC	2 cut(s)	327, 510
Eco31I	GGTCTC	1 cut(s)	830
Eco47I	GGWCC	2 cut(s)	131, 551
Eco88I	CYCGRG	1 cut(s)	968
Eco91I	GGTNACC	1 cut(s)	305
EcoO109I	RGGNCCY	1 cut(s)	584
EcoO65I	GGTNACC	1 cut(s)	305
EcoRII	CCWGG	2 cut(s)	301, 911
EcoT14I	CCWWGG	2 cut(s)	423, 672
EcoT22I	ATGCAT	1 cut(s)	532
EcoT38I	GRGCYC	2 cut(s)	327, 510
ErhI	CCWWGG	2 cut(s)	423, 672
FalI	AAGNNNNNCTT	2 cut(s)	843, 875
FaqI	GGGAC	3 cut(s)	258, 537, 885
FbaI	TGATCA	1 cut(s)	570
FblI	GTMKAC	2 cut(s)	267, 818
Fnu4HI	GCNGC	7 cut(s)	72, 467, 612, 847, 906, 909, 1004
FokI	GGATG	3 cut(s)	429, 746, 791
FriOI	GRGCYC	2 cut(s)	327, 510
Fsp4HI	GCNGC	7 cut(s)	72, 467, 612, 847, 906, 909, 1004
GlaI	GCGC	1 cut(s)	691
GluI	GCNGC	7 cut(s)	72, 467, 612, 847, 906, 909, 1004
GsaI	CCCAGC	1 cut(s)	261
GsuI	CTGGAG	1 cut(s)	885
HaeII	RGCGCY	1 cut(s)	693
HaeIII	GGCC	3 cut(s)	586, 611, 958
HapII	CCGG	7 cut(s)	145, 310, 327, 410, 843, 940, 1018
HgaI	GACGC	1 cut(s)	1088
HhaI	GCGC	1 cut(s)	692
Hin1I	GRCGYC	1 cut(s)	1080
Hin6I	GCGC	1 cut(s)	690
HinP1I	GCGC	1 cut(s)	690
HincII	GTYRAC	2 cut(s)	91, 819
HindII	GTYRAC	2 cut(s)	91, 819
HinfI	GANTC	2 cut(s)	148, 767
HpaII	CCGG	7 cut(s)	145, 310, 327, 410, 843, 940, 1018
HphI	GGTGA	5 cut(s)	250, 298, 317, 1007, 1088
Hpy166II	GTNNAC	4 cut(s)	91, 187, 268, 819
Hpy188I	TCNGA	7 cut(s)	131, 153, 435, 570, 578, 772, 866
Hpy188III	TCNNGA	4 cut(s)	100, 145, 196, 554
Hpy8I	GTNNAC	4 cut(s)	91, 187, 268, 819
Hpy99I	CGWCG	2 cut(s)	823, 998
HpyAV	CCTTC	2 cut(s)	485, 1030
HpyCH4III	ACNGT	4 cut(s)	345, 565, 703, 889
HpyCH4IV	ACGT	1 cut(s)	996
HpyCH4V	TGCA	3 cut(s)	357, 442, 530
HpyF10VI	GCNNNNNNNGC	8 cut(s)	41, 298, 617, 626, 696, 846, 855, 1064
HpyF3I	CTNAG	1 cut(s)	36
HpySE526I	ACGT	1 cut(s)	996
Hsp92I	GRCGYC	1 cut(s)	1080
HspAI	GCGC	1 cut(s)	690
Kpn2I	TCCGGA	1 cut(s)	144
Ksp22I	TGATCA	1 cut(s)	570
Kzo9I	GATC	5 cut(s)	102, 261, 451, 570, 990
LmnI	GCTCC	3 cut(s)	123, 279, 578
Lsp1109I	GCAGC	4 cut(s)	58, 453, 892, 895
LweI	GCATC	2 cut(s)	301, 451
MaeII	ACGT	1 cut(s)	996
MaeIII	GTNAC	3 cut(s)	305, 781, 1076
MalI	GATC	5 cut(s)	104, 263, 453, 572, 992
MbiI	CCGCTC	1 cut(s)	614
MboI	GATC	5 cut(s)	102, 261, 451, 570, 990
MboII	GAAGA	2 cut(s)	1000, 1058
MflI	RGATCY	2 cut(s)	102, 451
MhlI	GDGCHC	4 cut(s)	327, 510, 631, 1069
MlsI	TGGCCA	1 cut(s)	958
MluCI	AATT	1 cut(s)	636
MluNI	TGGCCA	1 cut(s)	958
MmeI	TCCRAC	2 cut(s)	499, 844
Mox20I	TGGCCA	1 cut(s)	958
Mph1103I	ATGCAT	1 cut(s)	532
MroI	TCCGGA	1 cut(s)	144
MroXI	GAANNNNTTC	1 cut(s)	395
MscI	TGGCCA	1 cut(s)	958
MseI	TTAA	5 cut(s)	156, 225, 753, 804, 1098
MslI	CAYNNNNRTG	2 cut(s)	252, 677
Msp20I	TGGCCA	1 cut(s)	958
MspA1I	CMGCKG	1 cut(s)	905
MspI	CCGG	7 cut(s)	145, 310, 327, 410, 843, 940, 1018
MspR9I	CCNGG	5 cut(s)	303, 311, 327, 843, 913
MvaI	CCWGG	2 cut(s)	303, 913
MwoI	GCNNNNNNNGC	8 cut(s)	41, 298, 617, 626, 696, 846, 855, 1064
NciI	CCSGG	3 cut(s)	311, 327, 843
NcoI	CCATGG	1 cut(s)	672
NdeII	GATC	5 cut(s)	102, 261, 451, 570, 990
NlaIV	GGNNCC	6 cut(s)	133, 300, 421, 553, 630, 700
NmeAIII	GCCGAG	1 cut(s)	666
NmuCI	GTSAC	3 cut(s)	305, 781, 1076
NsiI	ATGCAT	1 cut(s)	532
NspI	RCATGY	1 cut(s)	534
PaeR7I	CTCGAG	1 cut(s)	968
PdmI	GAANNNNTTC	1 cut(s)	395
PfeI	GAWTC	2 cut(s)	148, 767
PinAI	ACCGGT	2 cut(s)	409, 1017
PkrI	GCNGC	7 cut(s)	73, 468, 613, 848, 907, 910, 1005
Psp6I	CCWGG	2 cut(s)	301, 911
PspEI	GGTNACC	1 cut(s)	305
PspFI	CCCAGC	1 cut(s)	257
PspGI	CCWGG	2 cut(s)	301, 911
PspN4I	GGNNCC	6 cut(s)	133, 300, 421, 553, 630, 700
PspPI	GGNCC	3 cut(s)	131, 551, 584
PspXI	VCTCGAGB	1 cut(s)	968
PstNI	CAGNNNCTG	1 cut(s)	908
PsuI	RGATCY	2 cut(s)	102, 451
PvuII	CAGCTG	1 cut(s)	905
RsaI	GTAC	2 cut(s)	371, 944
RsaNI	GTAC	2 cut(s)	370, 943
RseI	CAYNNNNRTG	2 cut(s)	252, 677
SalI	GTCGAC	1 cut(s)	817
SaqAI	TTAA	5 cut(s)	156, 225, 753, 804, 1098
SatI	GCNGC	7 cut(s)	72, 467, 612, 847, 906, 909, 1004
Sau3AI	GATC	5 cut(s)	102, 261, 451, 570, 990
Sau96I	GGNCC	3 cut(s)	131, 551, 584
ScrFI	CCNGG	5 cut(s)	303, 311, 327, 843, 913
SduI	GDGCHC	4 cut(s)	327, 510, 631, 1069
SfaNI	GCATC	2 cut(s)	301, 451
SfcI	CTRYAG	1 cut(s)	1103
Sfr274I	CTCGAG	1 cut(s)	968
SinI	GGWCC	2 cut(s)	131, 551
SlaI	CTCGAG	1 cut(s)	968
SmiMI	CAYNNNNRTG	2 cut(s)	252, 677
SmlI	CTYRAG	3 cut(s)	194, 511, 968
SmoI	CTYRAG	3 cut(s)	194, 511, 968
Sse9I	AATT	1 cut(s)	636
SsiI	CCGC	7 cut(s)	161, 212, 317, 449, 612, 846, 1004
StyD4I	CCNGG	5 cut(s)	301, 309, 325, 841, 911
StyI	CCWWGG	2 cut(s)	423, 672
TaaI	ACNGT	4 cut(s)	345, 565, 703, 889
TaiI	ACGT	1 cut(s)	999
TaqI	TCGA	3 cut(s)	818, 969, 993
TasI	AATT	1 cut(s)	636
TatI	WGTACW	1 cut(s)	369
TauI	GCSGC	3 cut(s)	614, 849, 1006
TfiI	GAWTC	2 cut(s)	148, 767
Tru1I	TTAA	5 cut(s)	156, 225, 753, 804, 1098
Tru9I	TTAA	5 cut(s)	156, 225, 753, 804, 1098
TscAI	CASTG	3 cut(s)	359, 894, 1069
TseFI	GTSAC	3 cut(s)	305, 781, 1076
TseI	GCWGC	4 cut(s)	71, 466, 905, 908
Tsp45I	GTSAC	3 cut(s)	305, 781, 1076
TspDTI	ATGAA	4 cut(s)	218, 404, 404, 515
TspRI	CASTG	3 cut(s)	359, 894, 1069
VpaK11BI	GGWCC	2 cut(s)	131, 551
XceI	RCATGY	1 cut(s)	534
XhoI	CTCGAG	1 cut(s)	968
XmiI	GTMKAC	2 cut(s)	267, 818
XmnI	GAANNNNTTC	1 cut(s)	395
Zsp2I	ATGCAT	1 cut(s)	532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.