Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001681

Physical Location & Seq

Genomic Coordinates Reverse (-)

22283976 .. 22286706

Gene Structure 2731 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1110100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGGAAAGGGAGGCATGTCTGATGGAGATCATGCTGATCGCTGTTATGGGGAAGCAATAAATGGTGATGTTCAACAAGAGAACATGGCTGCTTGGCTTCTTGGTGTTAAAAACCTCAAGATTCAACCTTACAAGCTTCCTAATCTTGGACCCCATGATGTTAGAGTCCGGCTGAAGGCTGTTGGCATATGTGGCAGTGATGTTCACCACTTCAAGAACATGAGGTGTGTAGATTTTATAGTTAAAGAGCCAATGGTTATTGGGCATGAGTGTGCTGGGATCATAGAGGAAGTTGGGAGTGAGGTCGAGGATTTGGTGCCAGGCGATCGTGTGGCACTAGAGCCTGGTATCAGTTGCAAGCGATGCAACCTCTGCAAACAAGGCCGGTACAATCTATGCCGCAAGATGAAGTTTTTTGGCTCCCCTCCAAATAATGGTTGTCTGGCAAATCAGGTTGTCCATCCAGGAGATCTATGTTTTAAACTGCCAGACAATGTGAGTTTGGAGGAAGGCGCGATGTGTGAGCCCTTAAGTGTTGGTATTCATGCTTGTCGCCGGGCAAATGTCTGTCAAGAAACAAATGTCTTGGTCGTGGGAGCAGGACCTATAGGACTTGTTACACTGCTAGCCGCTCGTGCTTTTGGGGCGCCCCGAATTGTCATTGCGGATGTGAATGACGAGCGTTTGTTGATTGCAAAGAGTCTTGGCGCAGATGCAGTCGTTAAGGTTTCAACAAATATTGAGGATGTAGCTGAAGAAGTGGCTAAGATACAAAAGGTTTTGGAAAATGGAGTGGATGTAACCTTCGACTGTGCAGGCTTTAACAAAACCATAACAACAGCTTTGAGTGCTACTCGTCCCGGAGGCAAAGTTTGCCTTGTGGGAATGGGTCAGAGAGAGATGACTCTCCCTCTCGCTACCAGAGAGATTGATGTAATTGGAATTTTCCGATACCAGAACACATGGCCGCTGTGCCTTGAGTTTCTGAGAAGTGGTAAGATTGATGTGAAGCCCCTCATAACACATCGGTTTGGATTTTCTCAGAAGGAGGTGGAAGAAGCCTTTGAAACCAGTGCTCGCGGAGGCAATGCCATTAAGGTCATGTTTAACCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003939 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0008150 GO:0008152 GO:0009506 GO:0016491 GO:0016614 GO:0016616 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00040 ko00051 ko01100 map00040 map00051 map01100

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

372

Amino Acids

40.23

Weight (kDa)

6.47

Isoelectric Point (pI)

25.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	50 - 164	7e-29	Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C	PF16912	171 - 299	7.1e-11	Glucose dehydrogenase C-terminus
ADH_zinc_N	PF00107	202 - 329	3.2e-27	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	235 - 362	3.1e-08	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51970 AT5G51970
fragaria_vesca	FvH4_7g28870 FvH4_7g28870
malus_domestica	MD00G1005600.v1.1 MD00G1005700.v1.1 MD01G1110100.v1.1 MD01G1194600.v1.1 MD01G1194700.v1.1 MD01G1195200.v1.1 MD01G1195300.v1.1 MD01G1195400.v1.1 MD01G1195500.v1.1 MD07G1261900.v1.1 MD07G1262100.v1.1 MD07G1262300.v1.1
prunus_persica	Prupe.2G288800_v2.0.a1 Prupe.2G288800_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.4G240300_v2.0.a1 Prupe.8G142900_v2.0.a1 Prupe.8G143000_v2.0.a1
pyrus_communis	pycom01g13720 pycom01g20540 pycom01g20550 pycom01g20560 pycom01g20580 pycom01g20600 pycom01g20610 pycom07g24070 pycom07g24080 pycom07g24090 pycom07g24100 pycom07g24130
rosa_chinensis	RchiOBHm_Chr1g0376551
rosa_laevigata	RLG00000026602
rosa_multiflora	Rmu_sc0001308.1_g000011 Rmu_sc0005594.1_g000014
rosa_roxburghii	Rroxscaffold_4G00282010 Rroxscaffold_4G00282130
rosa_rugosa	Rorug01G0396500
rosa_samantha	Rh1AG407600 Rh1AG412800 Rh1BG372300 Rh1CG385100 Rh1CG386100 Rh1DG402100 Rh1DG403200
rosa_wichuraiana	Rw1G036180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	316, 646
AccB7I	CCANNNNNTGG	1 cut(s)	434
AccBSI	CCGCTC	1 cut(s)	632
AccII	CGCG	2 cut(s)	515, 1080
AciI	CCGC	5 cut(s)	400, 630, 665, 968, 1080
AclWI	GGATC	1 cut(s)	287
AcoI	YGGCCR	1 cut(s)	965
AcsI	RAATTY	1 cut(s)	942
AcuI	CTGAAG	2 cut(s)	194, 774
AcyI	GRCGYC	1 cut(s)	647
AfaI	GTAC	1 cut(s)	389
AfiI	CCNNNNNNNGG	3 cut(s)	146, 175, 434
AflII	CTTAAG	1 cut(s)	529
AgsI	TTSAA	5 cut(s)	74, 125, 214, 732, 1067
AjnI	CCWGG	3 cut(s)	319, 343, 463
AluBI	AGCT	3 cut(s)	136, 752, 842
AluI	AGCT	3 cut(s)	136, 752, 842
Alw21I	GWGCWC	1 cut(s)	1078
AlwI	GGATC	1 cut(s)	287
AoxI	GGCC	2 cut(s)	382, 965
ApeKI	GCWGC	1 cut(s)	89
ApoI	RAATTY	1 cut(s)	942
AspLEI	GCGC	3 cut(s)	515, 649, 710
AspS9I	GGNCC	2 cut(s)	149, 602
AsuC2I	CCSGG	2 cut(s)	557, 861
AsuHPI	GGTGA	2 cut(s)	77, 197
AsuNHI	GCTAGC	1 cut(s)	625
AvaII	GGWCC	2 cut(s)	149, 602
BanI	GGYRCC	2 cut(s)	316, 646
BanII	GRGCYC	1 cut(s)	528
BauI	CACGAG	1 cut(s)	633
Bbv12I	GWGCWC	1 cut(s)	1078
BbvI	GCAGC	1 cut(s)	76
BccI	CCATC	2 cut(s)	17, 468
BciT130I	CCWGG	3 cut(s)	321, 345, 465
BcnI	CCSGG	2 cut(s)	557, 861
BfaI	CTAG	2 cut(s)	338, 626
BfmI	CTRYAG	1 cut(s)	606
BfoI	RGCGCY	1 cut(s)	650
BfrI	CTTAAG	1 cut(s)	529
BglII	AGATCT	1 cut(s)	469
BisI	GCNGC	4 cut(s)	90, 400, 630, 968
BlsI	GCNGC	4 cut(s)	91, 401, 631, 969
Bme1390I	CCNGG	5 cut(s)	321, 345, 465, 557, 861
Bme18I	GGWCC	2 cut(s)	149, 602
BmgT120I	GGNCC	2 cut(s)	149, 602
BmiI	GGNNCC	4 cut(s)	151, 318, 421, 648
BmrFI	CCNGG	5 cut(s)	321, 345, 465, 557, 861
BmsI	GCATC	2 cut(s)	353, 703
BmtI	GCTAGC	1 cut(s)	629
BplI	GAGNNNNNCTC	2 cut(s)	838, 870
BpuEI	CTTGAG	2 cut(s)	101, 998
BpuMI	CCSGG	2 cut(s)	557, 861
BsaBI	GATNNNNATC	1 cut(s)	27
BsaHI	GRCGYC	1 cut(s)	647
BsaXI	ACNNNNNCTCC	4 cut(s)	783, 813, 855, 885
Bsc4I	CCNNNNNNNGG	3 cut(s)	146, 175, 434
Bse118I	RCCGGY	1 cut(s)	384
Bse1I	ACTGG	1 cut(s)	1071
Bse3DI	GCAATG	2 cut(s)	660, 1093
Bse8I	GATNNNNATC	1 cut(s)	27
BseBI	CCWGG	3 cut(s)	321, 345, 465
BseGI	GGATG	4 cut(s)	460, 673, 751, 802
BseJI	GATNNNNATC	1 cut(s)	27
BseLI	CCNNNNNNNGG	3 cut(s)	146, 175, 434
BseMI	GCAATG	2 cut(s)	660, 1093
BseMII	CTCAG	2 cut(s)	977, 1055
BseNI	ACTGG	1 cut(s)	1071
BseXI	GCAGC	1 cut(s)	76
BseYI	CCCAGC	1 cut(s)	275
BsgI	GTGCAG	1 cut(s)	834
Bsh1236I	CGCG	2 cut(s)	515, 1080
Bsh1285I	CGRYCG	1 cut(s)	328
BshFI	GGCC	2 cut(s)	384, 967
BshNI	GGYRCC	2 cut(s)	316, 646
BsiEI	CGRYCG	1 cut(s)	328
BsiHKAI	GWGCWC	1 cut(s)	1078
BsiSI	CCGG	4 cut(s)	169, 385, 556, 861
BslFI	GGGAC	1 cut(s)	843
BslI	CCNNNNNNNGG	3 cut(s)	146, 175, 434
BsmFI	GGGAC	1 cut(s)	843
BsnI	GGCC	2 cut(s)	384, 967
Bsp1286I	GDGCHC	2 cut(s)	528, 1078
Bsp143I	GATC	5 cut(s)	28, 37, 279, 325, 469
BspACI	CCGC	5 cut(s)	400, 630, 665, 968, 1080
BspANI	GGCC	2 cut(s)	384, 967
BspCNI	CTCAG	2 cut(s)	978, 1054
BspFNI	CGCG	2 cut(s)	515, 1080
BspLI	GGNNCC	4 cut(s)	151, 318, 421, 648
BspOI	GCTAGC	1 cut(s)	629
BspPI	GGATC	1 cut(s)	287
BspT107I	GGYRCC	2 cut(s)	316, 646
BspTI	CTTAAG	1 cut(s)	529
BsrBI	CCGCTC	1 cut(s)	632
BsrDI	GCAATG	2 cut(s)	660, 1093
BsrFI	RCCGGY	1 cut(s)	384
BsrI	ACTGG	1 cut(s)	1071
BssAI	RCCGGY	1 cut(s)	384
BssMI	GATC	5 cut(s)	28, 37, 279, 325, 469
BssNI	GRCGYC	1 cut(s)	647
BssSI	CACGAG	1 cut(s)	633
Bst2BI	CACGAG	1 cut(s)	633
Bst2UI	CCWGG	3 cut(s)	321, 345, 465
Bst4CI	ACNGT	1 cut(s)	812
BstACI	GRCGYC	1 cut(s)	647
BstAFI	CTTAAG	1 cut(s)	529
BstAPI	GCANNNNNTGC	3 cut(s)	363, 372, 873
BstC8I	GCNNGC	4 cut(s)	359, 627, 817, 1078
BstDEI	CTNAG	3 cut(s)	765, 986, 1041
BstF5I	GGATG	4 cut(s)	460, 673, 751, 802
BstFNI	CGCG	2 cut(s)	515, 1080
BstH2I	RGCGCY	1 cut(s)	650
BstHHI	GCGC	3 cut(s)	515, 649, 710
BstKTI	GATC	5 cut(s)	31, 40, 282, 328, 472
BstMBI	GATC	5 cut(s)	28, 37, 279, 325, 469
BstMCI	CGRYCG	1 cut(s)	328
BstMWI	GCNNNNNNNGC	8 cut(s)	192, 363, 372, 381, 635, 644, 848, 873
BstNI	CCWGG	3 cut(s)	321, 345, 465
BstNSI	RCATGY	1 cut(s)	19
BstSCI	CCNGG	5 cut(s)	319, 343, 463, 555, 859
BstSFI	CTRYAG	1 cut(s)	606
BstUI	CGCG	2 cut(s)	515, 1080
BstV1I	GCAGC	1 cut(s)	76
BstX2I	RGATCY	1 cut(s)	469
BstYI	RGATCY	1 cut(s)	469
BsuRI	GGCC	2 cut(s)	384, 967
BtgZI	GCGATG	2 cut(s)	376, 530
BtsCI	GGATG	4 cut(s)	460, 673, 751, 802
BtsI	GCAGTG	2 cut(s)	202, 620
BtsIMutI	CAGTG	3 cut(s)	202, 620, 1078
Cac8I	GCNNGC	4 cut(s)	359, 627, 817, 1078
CfoI	GCGC	3 cut(s)	515, 649, 710
Cfr10I	RCCGGY	1 cut(s)	384
Cfr13I	GGNCC	2 cut(s)	149, 602
Csp6I	GTAC	1 cut(s)	388
CviAII	CATG	9 cut(s)	16, 32, 85, 155, 220, 266, 545, 963, 1102
CviQI	GTAC	1 cut(s)	388
DdeI	CTNAG	3 cut(s)	765, 986, 1041
DinI	GGCGCC	1 cut(s)	648
DpnI	GATC	5 cut(s)	30, 39, 281, 327, 471
DpnII	GATC	5 cut(s)	28, 37, 279, 325, 469
DraI	TTTAAA	1 cut(s)	481
EaeI	YGGCCR	1 cut(s)	965
Eco24I	GRGCYC	1 cut(s)	528
Eco47I	GGWCC	2 cut(s)	149, 602
Eco57I	CTGAAG	2 cut(s)	194, 774
EcoO109I	RGGNCCY	1 cut(s)	602
EcoRII	CCWGG	3 cut(s)	319, 343, 463
EcoT38I	GRGCYC	1 cut(s)	528
EgeI	GGCGCC	1 cut(s)	648
EheI	GGCGCC	1 cut(s)	648
FaeI	CATG	9 cut(s)	19, 35, 88, 158, 223, 269, 548, 966, 1105
FalI	AAGNNNNNCTT	2 cut(s)	861, 893
FaqI	GGGAC	1 cut(s)	843
FatI	CATG	9 cut(s)	15, 31, 84, 154, 219, 265, 544, 962, 1101
FauNDI	CATATG	1 cut(s)	188
Fnu4HI	GCNGC	4 cut(s)	90, 400, 630, 968
FokI	GGATG	4 cut(s)	447, 680, 758, 809
FriOI	GRGCYC	1 cut(s)	528
Fsp4HI	GCNGC	4 cut(s)	90, 400, 630, 968
FspBI	CTAG	2 cut(s)	338, 626
GlaI	GCGC	3 cut(s)	514, 648, 709
GluI	GCNGC	4 cut(s)	90, 400, 630, 968
GsaI	CCCAGC	1 cut(s)	279
HaeII	RGCGCY	1 cut(s)	650
HaeIII	GGCC	2 cut(s)	384, 967
HapII	CCGG	4 cut(s)	169, 385, 556, 861
HhaI	GCGC	3 cut(s)	515, 649, 710
Hin1I	GRCGYC	1 cut(s)	647
Hin1II	CATG	9 cut(s)	19, 35, 88, 158, 223, 269, 548, 966, 1105
Hin6I	GCGC	3 cut(s)	513, 647, 708
HinP1I	GCGC	3 cut(s)	513, 647, 708
HindIII	AAGCTT	1 cut(s)	134
HinfI	GANTC	4 cut(s)	121, 165, 700, 904
HpaII	CCGG	4 cut(s)	169, 385, 556, 861
HphI	GGTGA	2 cut(s)	77, 197
Hpy166II	GTNNAC	1 cut(s)	205
Hpy188I	TCNGA	5 cut(s)	22, 894, 950, 987, 1044
Hpy188III	TCNNGA	3 cut(s)	118, 214, 572
Hpy8I	GTNNAC	1 cut(s)	205
HpyAV	CCTTC	4 cut(s)	169, 503, 814, 1039
HpyCH4III	ACNGT	1 cut(s)	812
HpyCH4V	TGCA	6 cut(s)	357, 366, 375, 695, 716, 815
HpyF10VI	GCNNNNNNNGC	8 cut(s)	192, 363, 372, 381, 635, 644, 848, 873
HpyF3I	CTNAG	3 cut(s)	765, 986, 1041
Hsp92I	GRCGYC	1 cut(s)	647
Hsp92II	CATG	9 cut(s)	19, 35, 88, 158, 223, 269, 548, 966, 1105
HspAI	GCGC	3 cut(s)	513, 647, 708
KasI	GGCGCC	1 cut(s)	646
Kzo9I	GATC	5 cut(s)	28, 37, 279, 325, 469
LmnI	GCTCC	2 cut(s)	425, 596
Lsp1109I	GCAGC	1 cut(s)	76
LweI	GCATC	2 cut(s)	353, 703
MaeI	CTAG	2 cut(s)	338, 626
MaeIII	GTNAC	2 cut(s)	616, 799
MalI	GATC	5 cut(s)	30, 39, 281, 327, 471
MbiI	CCGCTC	1 cut(s)	632
MboI	GATC	5 cut(s)	28, 37, 279, 325, 469
MboII	GAAGA	2 cut(s)	767, 1067
MflI	RGATCY	1 cut(s)	469
MhlI	GDGCHC	2 cut(s)	528, 1078
MluCI	AATT	3 cut(s)	654, 936, 942
Mly113I	GGCGCC	1 cut(s)	647
MlyI	GAGTC	3 cut(s)	174, 709, 898
MseI	TTAA	8 cut(s)	108, 243, 480, 530, 723, 822, 1095, 1107
MslI	CAYNNNNRTG	1 cut(s)	270
MspA1I	CMGCKG	1 cut(s)	970
MspCI	CTTAAG	1 cut(s)	529
MspI	CCGG	4 cut(s)	169, 385, 556, 861
MspR9I	CCNGG	5 cut(s)	321, 345, 465, 557, 861
MvaI	CCWGG	3 cut(s)	321, 345, 465
MvnI	CGCG	2 cut(s)	515, 1080
MwoI	GCNNNNNNNGC	8 cut(s)	192, 363, 372, 381, 635, 644, 848, 873
NarI	GGCGCC	1 cut(s)	647
NciI	CCSGG	2 cut(s)	557, 861
NdeI	CATATG	1 cut(s)	188
NdeII	GATC	5 cut(s)	28, 37, 279, 325, 469
NheI	GCTAGC	1 cut(s)	625
NlaIII	CATG	9 cut(s)	19, 35, 88, 158, 223, 269, 548, 966, 1105
NlaIV	GGNNCC	4 cut(s)	151, 318, 421, 648
NspI	RCATGY	1 cut(s)	19
PfeI	GAWTC	1 cut(s)	121
PflMI	CCANNNNNTGG	1 cut(s)	434
PfoI	TCCNGGA	2 cut(s)	463, 859
PkrI	GCNGC	4 cut(s)	91, 401, 631, 969
Ple19I	CGATCG	1 cut(s)	328
PleI	GAGTC	3 cut(s)	173, 708, 898
PluTI	GGCGCC	1 cut(s)	650
PpsI	GAGTC	3 cut(s)	173, 708, 898
PpuMI	RGGWCCY	1 cut(s)	602
Psp5II	RGGWCCY	1 cut(s)	602
Psp6I	CCWGG	3 cut(s)	319, 343, 463
PspFI	CCCAGC	1 cut(s)	275
PspGI	CCWGG	3 cut(s)	319, 343, 463
PspN4I	GGNNCC	4 cut(s)	151, 318, 421, 648
PspPI	GGNCC	2 cut(s)	149, 602
PspPPI	RGGWCCY	1 cut(s)	602
PsuI	RGATCY	1 cut(s)	469
PvuI	CGATCG	1 cut(s)	328
RsaI	GTAC	1 cut(s)	389
RsaNI	GTAC	1 cut(s)	388
RseI	CAYNNNNRTG	1 cut(s)	270
SaqAI	TTAA	8 cut(s)	108, 243, 480, 530, 723, 822, 1095, 1107
SatI	GCNGC	4 cut(s)	90, 400, 630, 968
Sau3AI	GATC	5 cut(s)	28, 37, 279, 325, 469
Sau96I	GGNCC	2 cut(s)	149, 602
SchI	GAGTC	3 cut(s)	174, 709, 898
ScrFI	CCNGG	5 cut(s)	321, 345, 465, 557, 861
SduI	GDGCHC	2 cut(s)	528, 1078
SfaNI	GCATC	2 cut(s)	353, 703
SfcI	CTRYAG	1 cut(s)	606
SfoI	GGCGCC	1 cut(s)	648
SinI	GGWCC	2 cut(s)	149, 602
SmiMI	CAYNNNNRTG	1 cut(s)	270
SmlI	CTYRAG	3 cut(s)	116, 529, 977
SmoI	CTYRAG	3 cut(s)	116, 529, 977
Sse9I	AATT	3 cut(s)	654, 936, 942
SsiI	CCGC	5 cut(s)	400, 630, 665, 968, 1080
SspDI	GGCGCC	1 cut(s)	646
SspI	AATATT	1 cut(s)	739
SspMI	CTAG	2 cut(s)	338, 626
StyD4I	CCNGG	5 cut(s)	319, 343, 463, 555, 859
TaaI	ACNGT	1 cut(s)	812
TaqI	TCGA	2 cut(s)	306, 807
TasI	AATT	3 cut(s)	654, 936, 942
TauI	GCSGC	3 cut(s)	402, 632, 970
TfiI	GAWTC	1 cut(s)	121
Tru1I	TTAA	8 cut(s)	108, 243, 480, 530, 723, 822, 1095, 1107
Tru9I	TTAA	8 cut(s)	108, 243, 480, 530, 723, 822, 1095, 1107
TscAI	CASTG	3 cut(s)	202, 627, 1078
TseI	GCWGC	1 cut(s)	89
TspDTI	ATGAA	2 cut(s)	422, 533
TspRI	CASTG	3 cut(s)	202, 627, 1078
Van91I	CCANNNNNTGG	1 cut(s)	434
Vha464I	CTTAAG	1 cut(s)	529
VpaK11BI	GGWCC	2 cut(s)	149, 602
XapI	RAATTY	1 cut(s)	942
XceI	RCATGY	1 cut(s)	19
XspI	CTAG	2 cut(s)	338, 626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.