pycom07g24100

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001681
Physical Location & Seq
Forward (+)
24892851 .. 24894091
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24100.1

Sequence Viewer

Length: 1110 bp
ATGGGTAAGGGAGGTCAATCCTGCAATGGCGTGGTTAGAGATGCCAAACCTGTTGAGCAGGAAAACATGGCTGCCTGGCTCGTTGATGTTAACACCATCAGGATCCTACCTTTCAAGCTCCCCGATATTGGACCCAATGATGTTCAAATTCGGATTAAGGCTGTCGGCATTTGTGGAAGCGATGTTCACTACCTCAAGAACATGAAACTGGCGGATTTTGAGGTGAAGGAACCAATGGTGATCGGACATGAGTGTGCAGGATCGATCGTAGAAAAAGTTGGGAGCGACGTGAAGCATCTGGTTTCTGGTGACCGCGTGGCGGTAGAGCCCGGCATCAGCTGCTCACGTTGCCAGCAGTGCAAAGGAGGCCGCTACAATCTCTGCCCCGACATGAAGTTTTTCGCCACCCCACCGGTTCATGGTTCCTTGGCTAACCAGATTGTGCACCCTGCAGATCTATGCTTTAAACTGCCGGAAAACGTGAGCTTGGAGGAAGGAGCAATGTGTGAGCCCTTGAGTGTTGGGGTTCACGCTTGTCGGCGAGCCAATGTTGGTCCCGAAACAACTGTTCTGATCGTCGGAGCAGGGCCGATTGGGCTGGTTTCCGTGCTCGCTGCTCGTGCCTTCGGAGCACCAAGAATCGTCATCGTAGATATGGATGATAAGCGTTTAGCCATGGCAAAGTCTCTGGGAGCTGATGGAACCGTCAAAGTTTCGGCAAAAATGGAGGATTTGGATGACGAAGTTGCCAAGATTAAAGAAACCATGGGAGCCGAAGTTGATGTGACCTTCGACTGTGTGGGTTTCAACAAAACCATGTCTACGGGCCTCAATGCTACTCGTCCCGGCGGCAAAGTTTGCCTTGTAGGAATGGGGCACAGCATGATGACAGTCCCTCTGACTCCGGCTGCCGCCAGGGAGGTTGACGTGGTTGGAGTTTTCCGGTATAAGAACACATGGCCTCTTTGCCTAGAGTTTTTGAGAAGCGGGAAGATCGACGTGAAGCCGCTTATTACCCACCGGTTTGAATTTACCGAGAAGGAGGTGGAGGAAGCATTTGCAACCAGTGCTCGTGGGGGTGACGCCATTAAAGTCATGTTTAATCTATAG

Protein Analysis

370

Amino Acids

39.61

Weight (kDa)

6.27

Isoelectric Point (pI)

24.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 44 - 159 6.5e-27 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 166 - 298 6.9e-09 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 197 - 327 4.6e-29 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 245 - 359 2.1e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 821
AccII CGCG 1 cut(s) 315
AciI CCGC 8 cut(s) 212, 313, 320, 370, 849, 912, 987, 1007
AclWI GGATC 3 cut(s) 97, 110, 268
AcsI RAATTY 2 cut(s) 147, 1028
AcyI GRCGYC 1 cut(s) 1083
AfiI CCNNNNNNNGG 3 cut(s) 128, 319, 419
AgeI ACCGGT 2 cut(s) 412, 1020
AgsI TTSAA 4 cut(s) 115, 146, 808, 1028
AjiI CACGTC 3 cut(s) 289, 928, 1000
AjnI CCWGG 2 cut(s) 74, 914
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 4 cut(s) 118, 339, 486, 695
AluI AGCT 4 cut(s) 118, 339, 486, 695
Alw21I GWGCWC 4 cut(s) 447, 612, 634, 1072
Alw26I GTCTC 1 cut(s) 690
Alw44I GTGCAC 1 cut(s) 443
AlwI GGATC 3 cut(s) 97, 110, 268
AoxI GGCC 4 cut(s) 367, 587, 826, 959
ApaLI GTGCAC 1 cut(s) 443
ApeKI GCWGC 4 cut(s) 71, 339, 614, 908
ApoI RAATTY 2 cut(s) 147, 1028
AsiGI ACCGGT 2 cut(s) 412, 1020
Asp700I GAANNNNTTC 1 cut(s) 398
AspS9I GGNCC 4 cut(s) 131, 554, 587, 826
AsuC2I CCSGG 2 cut(s) 330, 846
AsuHPI GGTGA 4 cut(s) 235, 250, 320, 1091
AvaII GGWCC 2 cut(s) 131, 554
BaeGI GKGCMC 2 cut(s) 447, 879
BamHI GGATCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 330, 513
BauI CACGAG 2 cut(s) 618, 1071
Bbv12I GWGCWC 4 cut(s) 447, 612, 634, 1072
BbvI GCAGC 4 cut(s) 58, 326, 601, 895
BccI CCATC 2 cut(s) 104, 692
BciT130I CCWGG 2 cut(s) 76, 916
BcnI CCSGG 2 cut(s) 330, 846
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 1 cut(s) 971
BfmI CTRYAG 2 cut(s) 450, 1106
BglI GCCNNNNNGGC 1 cut(s) 595
BglII AGATCT 1 cut(s) 454
BisI GCNGC 8 cut(s) 72, 340, 370, 615, 850, 909, 912, 1007
BlsI GCNGC 8 cut(s) 73, 341, 371, 616, 851, 910, 913, 1008
Bme1390I CCNGG 4 cut(s) 76, 330, 846, 916
Bme18I GGWCC 2 cut(s) 131, 554
BmgBI CACGTC 3 cut(s) 289, 928, 1000
BmgT120I GGNCC 4 cut(s) 131, 554, 587, 826
BmiI GGNNCC 7 cut(s) 104, 133, 231, 424, 556, 703, 772
BmrFI CCNGG 4 cut(s) 76, 330, 846, 916
BmsI GCATC 3 cut(s) 31, 304, 342
BpuEI CTTGAG 2 cut(s) 179, 535
BpuMI CCSGG 2 cut(s) 330, 846
Bsa29I ATCGAT 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 1083
BsaJI CCNNGG 4 cut(s) 426, 675, 765, 915
BsaWI WCCGGW 3 cut(s) 412, 942, 1020
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 319, 419
Bse118I RCCGGY 2 cut(s) 412, 1020
Bse1I ACTGG 2 cut(s) 213, 1065
Bse3DI GCAATG 2 cut(s) 31, 507
BseBI CCWGG 2 cut(s) 76, 916
BseCI ATCGAT 1 cut(s) 263
BseDI CCNNGG 4 cut(s) 426, 675, 765, 915
BseGI GGATG 2 cut(s) 664, 742
BseLI CCNNNNNNNGG 3 cut(s) 128, 319, 419
BseMI GCAATG 2 cut(s) 31, 507
BseNI ACTGG 2 cut(s) 213, 1065
BseSI GKGCMC 2 cut(s) 447, 879
BseXI GCAGC 4 cut(s) 58, 326, 601, 895
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 4 cut(s) 369, 589, 828, 961
BshTI ACCGGT 2 cut(s) 412, 1020
BshVI ATCGAT 1 cut(s) 263
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 4 cut(s) 447, 612, 634, 1072
BsiSI CCGG 7 cut(s) 330, 413, 473, 846, 905, 943, 1021
BslFI GGGAC 3 cut(s) 540, 828, 878
BslI CCNNNNNNNGG 3 cut(s) 128, 319, 419
BsmAI GTCTC 1 cut(s) 690
BsmFI GGGAC 3 cut(s) 540, 828, 878
BsnI GGCC 4 cut(s) 369, 589, 828, 961
Bsp1286I GDGCHC 7 cut(s) 330, 447, 513, 612, 634, 879, 1072
Bsp143I GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
Bsp19I CCATGG 2 cut(s) 675, 765
BspACI CCGC 8 cut(s) 212, 313, 320, 370, 849, 912, 987, 1007
BspANI GGCC 4 cut(s) 369, 589, 828, 961
BspDI ATCGAT 1 cut(s) 263
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 7 cut(s) 104, 133, 231, 424, 556, 703, 772
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 3 cut(s) 97, 110, 268
BsrDI GCAATG 2 cut(s) 31, 507
BsrFI RCCGGY 2 cut(s) 412, 1020
BsrI ACTGG 2 cut(s) 213, 1065
BssAI RCCGGY 2 cut(s) 412, 1020
BssECI CCNNGG 4 cut(s) 426, 675, 765, 915
BssMI GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
BssNI GRCGYC 1 cut(s) 1083
BssSI CACGAG 2 cut(s) 618, 1071
BssT1I CCWWGG 3 cut(s) 426, 675, 765
Bst2BI CACGAG 2 cut(s) 618, 1071
Bst2UI CCWGG 2 cut(s) 76, 916
Bst4CI ACNGT 4 cut(s) 568, 706, 797, 892
BstACI GRCGYC 1 cut(s) 1083
BstAPI GCANNNNNTGC 3 cut(s) 339, 858, 1067
BstC8I GCNNGC 3 cut(s) 353, 543, 612
BstDSI CCRYGG 2 cut(s) 675, 765
BstEII GGTNACC 1 cut(s) 308
BstF5I GGATG 2 cut(s) 664, 742
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 7 cut(s) 105, 243, 263, 267, 457, 576, 996
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 9 cut(s) 339, 348, 357, 366, 595, 620, 629, 858, 1067
BstNI CCWGG 2 cut(s) 76, 916
BstPI GGTNACC 1 cut(s) 308
BstSCI CCNGG 4 cut(s) 74, 328, 844, 914
BstSFI CTRYAG 2 cut(s) 450, 1106
BstSLI GKGCMC 2 cut(s) 447, 879
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 4 cut(s) 58, 326, 601, 895
BstX2I RGATCY 2 cut(s) 102, 454
BstYI RGATCY 2 cut(s) 102, 454
Bsu15I ATCGAT 1 cut(s) 263
BsuRI GGCC 4 cut(s) 369, 589, 828, 961
BsuTUI ATCGAT 1 cut(s) 263
BtgI CCRYGG 2 cut(s) 675, 765
BtgZI GCGATG 1 cut(s) 195
BtrI CACGTC 3 cut(s) 289, 928, 1000
BtsCI GGATG 2 cut(s) 664, 742
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 2 cut(s) 362, 1072
Cac8I GCNNGC 3 cut(s) 353, 543, 612
Cfr10I RCCGGY 2 cut(s) 412, 1020
Cfr13I GGNCC 4 cut(s) 131, 554, 587, 826
ClaI ATCGAT 1 cut(s) 263
CseI GACGC 1 cut(s) 1091
CspAI ACCGGT 2 cut(s) 412, 1020
DpnI GATC 7 cut(s) 104, 242, 262, 266, 456, 575, 995
DpnII GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
DraI TTTAAA 1 cut(s) 466
EciI GGCGGA 1 cut(s) 227
Eco130I CCWWGG 3 cut(s) 426, 675, 765
Eco24I GRGCYC 2 cut(s) 330, 513
Eco47I GGWCC 2 cut(s) 131, 554
Eco91I GGTNACC 1 cut(s) 308
EcoO65I GGTNACC 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 74, 914
EcoT14I CCWWGG 3 cut(s) 426, 675, 765
EcoT38I GRGCYC 2 cut(s) 330, 513
ErhI CCWWGG 3 cut(s) 426, 675, 765
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 3 cut(s) 540, 828, 878
FauI CCCGC 1 cut(s) 980
FblI GTMKAC 1 cut(s) 821
Fnu4HI GCNGC 8 cut(s) 72, 340, 370, 615, 850, 909, 912, 1007
FokI GGATG 2 cut(s) 671, 749
FriOI GRGCYC 2 cut(s) 330, 513
Fsp4HI GCNGC 8 cut(s) 72, 340, 370, 615, 850, 909, 912, 1007
FspBI CTAG 1 cut(s) 971
GluI GCNGC 8 cut(s) 72, 340, 370, 615, 850, 909, 912, 1007
HaeIII GGCC 4 cut(s) 369, 589, 828, 961
HapII CCGG 7 cut(s) 330, 413, 473, 846, 905, 943, 1021
HgaI GACGC 1 cut(s) 1091
Hin1I GRCGYC 1 cut(s) 1083
HincII GTYRAC 2 cut(s) 91, 925
HindII GTYRAC 2 cut(s) 91, 925
HinfI GANTC 2 cut(s) 639, 901
HpaI GTTAAC 1 cut(s) 91
HpaII CCGG 7 cut(s) 330, 413, 473, 846, 905, 943, 1021
HphI GGTGA 4 cut(s) 235, 250, 320, 1091
Hpy166II GTNNAC 6 cut(s) 91, 187, 445, 529, 822, 925
Hpy188I TCNGA 6 cut(s) 153, 245, 573, 581, 629, 900
Hpy188III TCNNGA 3 cut(s) 100, 196, 557
Hpy8I GTNNAC 6 cut(s) 91, 187, 445, 529, 822, 925
Hpy99I CGWCG 3 cut(s) 290, 581, 1001
HpyAV CCTTC 5 cut(s) 220, 488, 634, 799, 1033
HpyCH4III ACNGT 4 cut(s) 568, 706, 797, 892
HpyCH4IV ACGT 5 cut(s) 288, 346, 480, 927, 999
HpyCH4V TGCA 6 cut(s) 24, 257, 360, 445, 452, 1061
HpyF10VI GCNNNNNNNGC 9 cut(s) 339, 348, 357, 366, 595, 620, 629, 858, 1067
HpySE526I ACGT 5 cut(s) 288, 346, 480, 927, 999
Hsp92I GRCGYC 1 cut(s) 1083
KspAI GTTAAC 1 cut(s) 91
Kzo9I GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
LmnI GCTCC 7 cut(s) 123, 282, 497, 581, 629, 692, 770
Lsp1109I GCAGC 4 cut(s) 58, 326, 601, 895
LweI GCATC 3 cut(s) 31, 304, 342
MaeI CTAG 1 cut(s) 971
MaeII ACGT 5 cut(s) 288, 346, 480, 927, 999
MaeIII GTNAC 3 cut(s) 308, 784, 1079
MalI GATC 7 cut(s) 104, 242, 262, 266, 456, 575, 995
MboI GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
MboII GAAGA 1 cut(s) 1003
MflI RGATCY 2 cut(s) 102, 454
MhlI GDGCHC 7 cut(s) 330, 447, 513, 612, 634, 879, 1072
MluCI AATT 2 cut(s) 147, 1028
MlyI GAGTC 1 cut(s) 895
MmeI TCCRAC 2 cut(s) 559, 913
MroXI GAANNNNTTC 1 cut(s) 398
MseI TTAA 6 cut(s) 90, 156, 465, 756, 1089, 1101
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 7 cut(s) 330, 413, 473, 846, 905, 943, 1021
MspR9I CCNGG 4 cut(s) 76, 330, 846, 916
MvaI CCWGG 2 cut(s) 76, 916
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 9 cut(s) 339, 348, 357, 366, 595, 620, 629, 858, 1067
NciI CCSGG 2 cut(s) 330, 846
NcoI CCATGG 2 cut(s) 675, 765
NdeII GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
NlaIV GGNNCC 7 cut(s) 104, 133, 231, 424, 556, 703, 772
NmuCI GTSAC 3 cut(s) 308, 784, 1079
PdmI GAANNNNTTC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 639
PinAI ACCGGT 2 cut(s) 412, 1020
PkrI GCNGC 8 cut(s) 73, 341, 371, 616, 851, 910, 913, 1008
Ple19I CGATCG 1 cut(s) 267
PleI GAGTC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 895
Psp6I CCWGG 2 cut(s) 74, 914
PspEI GGTNACC 1 cut(s) 308
PspGI CCWGG 2 cut(s) 74, 914
PspN4I GGNNCC 7 cut(s) 104, 133, 231, 424, 556, 703, 772
PspPI GGNCC 4 cut(s) 131, 554, 587, 826
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 2 cut(s) 102, 454
PvuI CGATCG 1 cut(s) 267
PvuII CAGCTG 1 cut(s) 339
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 6 cut(s) 90, 156, 465, 756, 1089, 1101
SatI GCNGC 8 cut(s) 72, 340, 370, 615, 850, 909, 912, 1007
Sau3AI GATC 7 cut(s) 102, 240, 260, 264, 454, 573, 993
Sau96I GGNCC 4 cut(s) 131, 554, 587, 826
SchI GAGTC 1 cut(s) 895
ScrFI CCNGG 4 cut(s) 76, 330, 846, 916
SduI GDGCHC 7 cut(s) 330, 447, 513, 612, 634, 879, 1072
SfaNI GCATC 3 cut(s) 31, 304, 342
SfcI CTRYAG 2 cut(s) 450, 1106
SinI GGWCC 2 cut(s) 131, 554
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 194, 514
SmoI CTYRAG 2 cut(s) 194, 514
Sse9I AATT 2 cut(s) 147, 1028
SsiI CCGC 8 cut(s) 212, 313, 320, 370, 849, 912, 987, 1007
SspMI CTAG 1 cut(s) 971
StyD4I CCNGG 4 cut(s) 74, 328, 844, 914
StyI CCWWGG 3 cut(s) 426, 675, 765
TaaI ACNGT 4 cut(s) 568, 706, 797, 892
TaiI ACGT 5 cut(s) 291, 349, 483, 930, 1002
TaqI TCGA 3 cut(s) 263, 792, 996
TasI AATT 2 cut(s) 147, 1028
TauI GCSGC 4 cut(s) 372, 852, 914, 1009
TfiI GAWTC 1 cut(s) 639
Tru1I TTAA 6 cut(s) 90, 156, 465, 756, 1089, 1101
Tru9I TTAA 6 cut(s) 90, 156, 465, 756, 1089, 1101
TscAI CASTG 2 cut(s) 362, 1072
TseFI GTSAC 3 cut(s) 308, 784, 1079
TseI GCWGC 4 cut(s) 71, 339, 614, 908
Tsp45I GTSAC 3 cut(s) 308, 784, 1079
TspDTI ATGAA 3 cut(s) 218, 407, 407
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 2 cut(s) 362, 1072
VneI GTGCAC 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 131, 554
XapI RAATTY 2 cut(s) 147, 1028
XmiI GTMKAC 1 cut(s) 821
XmnI GAANNNNTTC 1 cut(s) 398
XspI CTAG 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.