MD03G1012700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002716
Physical Location & Seq
Reverse (-)
966462 .. 968391
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1012700.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGAACATGATGCATTCTTCTTGTAGCGCTCAATTTTGTTACAAATATATGTTTGTAATATTACTAATCTTGTGTGCAAGTGCAGGATCTGGTGATGCTCAGTTGACTCCTACGTTTTATGATGAATCATGCCCGAATGCCACCAGCATTGTGCGTGGAGTCATTCAAGAGGCTCTGCAGACCGATCCCCGGATTGCTGCCAGCCTCATTCGGCTTCACTTCCACGATTGCTTTGTCAACGGTTGCGATGGATCAATTCTGTTGGACAATAGTACCAGTTCTACCAGTACCATAGACAGCGAAAAAACAGCATTTGCAAATAACAACTCAGCCAGAGGATTTGATGTTGTGGACAGTATCAAGACTGCATTGGAGAATGCTTGTCCCGCCATCGTTTCCTGTGCTGATATTCTCACCATTGCTGCCGAAGAATCTGTTGCTTTGTCTGGAGGTCCTTCATGGACAGTGCTACTGGGAAGAAGGGATAGCACAACAGCGAACCGGACGGCTGCCAACGCAGCCATTCCAGCTCCTACTTTAACACTTGATGGACTCAAGGCCAACTTCTTAGCTGTAGGCCTCAACACCACTGATCTGGTTGCACTATCTGGTGCTCACACATTTGGGCGTGCTAGATGTCAATCTTTCACCAATCGATTGTATAACTTCAGCGGCACAGGCAGTCCTGATCCAACCTTGAACTCAACCTACTTAGAAACACTGAGTGAGATATGCCCGCAGAATGGGAATAGCAATGTGCTAGCCAATTTGGATCCTGTAACGCCCGACACTTTTGATGCAAAGTATTTCTCGAATCTTCAAGTCCAGAAAGGCTTGCTCCAAAGCGATCAAGAGCTGTTTTCAACTAGCGGGGCTGATACCACCGACATTGTTAACAACTTCAGCACTAATCAGAGCGCATTCTTTGAGAGTTTCGTGGAATCCATGATTAAAATGGGGAATATAAGCCCCTTGACCGGTACTGATGGAGAGATTCGATTGAACTGCCGTAGGGTTAATGGAGATTCATATGGACCAGCTGCTACTTTGGTAGCTGAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0020037 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0046906 GO:0048037 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097159 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901363 GO:1990748
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

355

Amino Acids

37.69

Weight (kDa)

4.55

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 51 - 303 1.7e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 387, 672, 737, 870
AclWI GGATC 6 cut(s) 94, 179, 259, 683, 767, 780
AcuI CTGAAG 2 cut(s) 652, 886
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 3 cut(s) 274, 289, 982
AfeI AGCGCT 1 cut(s) 28
AfiI CCNNNNNNNGG 3 cut(s) 189, 743, 977
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 5 cut(s) 167, 700, 821, 864, 1003
AluBI AGCT 5 cut(s) 530, 572, 856, 1040, 1055
AluI AGCT 5 cut(s) 530, 572, 856, 1040, 1055
Alw21I GWGCWC 1 cut(s) 616
AlwI GGATC 6 cut(s) 94, 179, 259, 683, 767, 780
AlwNI CAGNNNCTG 1 cut(s) 89
Aor51HI AGCGCT 1 cut(s) 28
AoxI GGCC 2 cut(s) 558, 577
ApeKI GCWGC 5 cut(s) 197, 422, 509, 518, 1040
AsiGI ACCGGT 1 cut(s) 977
AspLEI GCGC 2 cut(s) 29, 920
AspS9I GGNCC 2 cut(s) 452, 1034
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 3 cut(s) 104, 406, 640
AsuNHI GCTAGC 1 cut(s) 760
AvaII GGWCC 2 cut(s) 452, 1034
BaeI ACNNNNGTAYC 2 cut(s) 256, 289
BamHI GGATCC 1 cut(s) 772
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 5 cut(s) 184, 409, 496, 530, 1027
BccI CCATC 4 cut(s) 242, 398, 542, 980
BceAI ACGGC 2 cut(s) 522, 993
BcgI CGANNNNNNTGC 2 cut(s) 987, 1021
BcnI CCSGG 1 cut(s) 190
BfaI CTAG 3 cut(s) 633, 761, 867
BfmI CTRYAG 2 cut(s) 176, 573
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 6 cut(s) 198, 423, 510, 519, 673, 1041
BlsI GCNGC 6 cut(s) 199, 424, 511, 520, 674, 1042
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 2 cut(s) 452, 1034
BmgT120I GGNCC 2 cut(s) 452, 1034
BmiI GGNNCC 1 cut(s) 774
BmrFI CCNGG 1 cut(s) 190
BmrI ACTGGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 85, 787
BmtI GCTAGC 1 cut(s) 764
BmuI ACTGGG 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 190
Bsa29I ATCGAT 1 cut(s) 655
BsaBI GATNNNNATC 1 cut(s) 640
BsaJI CCNNGG 1 cut(s) 188
BsaWI WCCGGW 2 cut(s) 501, 977
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 743, 977
Bse118I RCCGGY 1 cut(s) 977
Bse1I ACTGG 3 cut(s) 276, 285, 477
Bse3DI GCAATG 2 cut(s) 417, 760
Bse8I GATNNNNATC 1 cut(s) 640
BseCI ATCGAT 1 cut(s) 655
BseDI CCNNGG 1 cut(s) 188
BseJI GATNNNNATC 1 cut(s) 640
BseLI CCNNNNNNNGG 3 cut(s) 189, 743, 977
BseMI GCAATG 2 cut(s) 417, 760
BseMII CTCAG 3 cut(s) 113, 342, 713
BseNI ACTGG 3 cut(s) 276, 285, 477
BseXI GCAGC 5 cut(s) 184, 409, 496, 530, 1027
BsgI GTGCAG 1 cut(s) 102
BshFI GGCC 2 cut(s) 560, 579
BshTI ACCGGT 1 cut(s) 977
BshVI ATCGAT 1 cut(s) 655
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 3 cut(s) 190, 502, 978
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 189, 743, 977
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 4 cut(s) 13, 142, 382, 920
BsnI GGCC 2 cut(s) 560, 579
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
BspACI CCGC 4 cut(s) 387, 672, 737, 870
BspANI GGCC 2 cut(s) 560, 579
BspCNI CTCAG 3 cut(s) 112, 341, 714
BspDI ATCGAT 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 774
BspMAI CTGCAG 1 cut(s) 180
BspOI GCTAGC 1 cut(s) 764
BspPI GGATC 6 cut(s) 94, 179, 259, 683, 767, 780
BsrDI GCAATG 2 cut(s) 417, 760
BsrFI RCCGGY 1 cut(s) 977
BsrI ACTGG 3 cut(s) 276, 285, 477
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
Bst4CI ACNGT 3 cut(s) 242, 356, 466
BstC8I GCNNGC 5 cut(s) 202, 630, 737, 762, 836
BstDEI CTNAG 5 cut(s) 99, 328, 568, 712, 722
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 29, 920
BstKTI GATC 7 cut(s) 89, 187, 254, 595, 691, 775, 850
BstMBI GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
BstMWI GCNNNNNNNGC 5 cut(s) 386, 515, 518, 527, 678
BstSCI CCNGG 1 cut(s) 188
BstSFI CTRYAG 2 cut(s) 176, 573
BstV1I GCAGC 5 cut(s) 184, 409, 496, 530, 1027
BstX2I RGATCY 2 cut(s) 86, 772
BstXI CCANNNNNNTGG 1 cut(s) 595
BstYI RGATCY 2 cut(s) 86, 772
Bsu15I ATCGAT 1 cut(s) 655
BsuRI GGCC 2 cut(s) 560, 579
BsuTUI ATCGAT 1 cut(s) 655
BtgZI GCGATG 1 cut(s) 261
BtsIMutI CAGTG 3 cut(s) 471, 588, 719
Cac8I GCNNGC 5 cut(s) 202, 630, 737, 762, 836
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 2 cut(s) 29, 920
Cfr10I RCCGGY 1 cut(s) 977
Cfr13I GGNCC 2 cut(s) 452, 1034
ClaI ATCGAT 1 cut(s) 655
Csp6I GTAC 3 cut(s) 273, 288, 981
CspAI ACCGGT 1 cut(s) 977
CspCI CAANNNNNGTGG 4 cut(s) 130, 165, 871, 906
CviAII CATG 4 cut(s) 7, 129, 459, 946
CviQI GTAC 3 cut(s) 273, 288, 981
DdeI CTNAG 5 cut(s) 99, 328, 568, 712, 722
DpnI GATC 7 cut(s) 88, 186, 253, 594, 690, 774, 849
DpnII GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
DraIII CACNNNGTG 1 cut(s) 725
Eco147I AGGCCT 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 452, 1034
Eco47III AGCGCT 1 cut(s) 28
Eco57I CTGAAG 2 cut(s) 652, 886
EcoO109I RGGNCCY 1 cut(s) 452
EcoT22I ATGCAT 1 cut(s) 15
FaeI CATG 4 cut(s) 10, 132, 462, 949
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 1 cut(s) 369
FatI CATG 4 cut(s) 6, 128, 458, 945
FauI CCCGC 3 cut(s) 394, 744, 863
FauNDI CATATG 1 cut(s) 1030
Fnu4HI GCNGC 6 cut(s) 198, 423, 510, 519, 673, 1041
Fsp4HI GCNGC 6 cut(s) 198, 423, 510, 519, 673, 1041
FspBI CTAG 3 cut(s) 633, 761, 867
GlaI GCGC 2 cut(s) 28, 919
GluI GCNGC 6 cut(s) 198, 423, 510, 519, 673, 1041
GsuI CTGGAG 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 560, 579
HapII CCGG 3 cut(s) 190, 502, 978
HhaI GCGC 2 cut(s) 29, 920
Hin1II CATG 4 cut(s) 10, 132, 462, 949
Hin6I GCGC 2 cut(s) 27, 918
HinP1I GCGC 2 cut(s) 27, 918
HincII GTYRAC 3 cut(s) 105, 238, 895
HindII GTYRAC 3 cut(s) 105, 238, 895
HinfI GANTC 9 cut(s) 106, 125, 159, 431, 552, 814, 941, 994, 1025
HpaI GTTAAC 1 cut(s) 895
HpaII CCGG 3 cut(s) 190, 502, 978
HphI GGTGA 3 cut(s) 104, 406, 640
Hpy166II GTNNAC 4 cut(s) 105, 238, 352, 895
Hpy188I TCNGA 1 cut(s) 915
Hpy188III TCNNGA 7 cut(s) 167, 361, 447, 686, 811, 826, 851
Hpy8I GTNNAC 4 cut(s) 105, 238, 352, 895
HpyAV CCTTC 2 cut(s) 465, 474
HpyCH4III ACNGT 3 cut(s) 242, 356, 466
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 8 cut(s) 13, 77, 83, 178, 317, 368, 602, 800
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 515, 518, 527, 678
HpyF3I CTNAG 5 cut(s) 99, 328, 568, 712, 722
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 4 cut(s) 10, 132, 462, 949
HspAI GCGC 2 cut(s) 27, 918
KspAI GTTAAC 1 cut(s) 895
Kzo9I GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
LmnI GCTCC 2 cut(s) 535, 843
Lsp1109I GCAGC 5 cut(s) 184, 409, 496, 530, 1027
LweI GCATC 2 cut(s) 85, 787
MaeI CTAG 3 cut(s) 633, 761, 867
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 38, 778
MalI GATC 7 cut(s) 88, 186, 253, 594, 690, 774, 849
MboI GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
MboII GAAGA 4 cut(s) 9, 440, 489, 809
MflI RGATCY 2 cut(s) 86, 772
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 3 cut(s) 32, 255, 766
MlyI GAGTC 3 cut(s) 100, 168, 546
MmeI TCCRAC 2 cut(s) 243, 716
MnlI CCTC 5 cut(s) 163, 215, 329, 443, 590
Mph1103I ATGCAT 1 cut(s) 15
MseI TTAA 5 cut(s) 539, 894, 951, 1017, 1063
MspA1I CMGCKG 2 cut(s) 672, 1040
MspI CCGG 3 cut(s) 190, 502, 978
MspR9I CCNGG 1 cut(s) 190
Mva1269I GAATGC 4 cut(s) 13, 142, 382, 920
MwoI GCNNNNNNNGC 5 cut(s) 386, 515, 518, 527, 678
NciI CCSGG 1 cut(s) 190
NdeI CATATG 1 cut(s) 1030
NdeII GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
NheI GCTAGC 1 cut(s) 760
NlaIII CATG 4 cut(s) 10, 132, 462, 949
NlaIV GGNNCC 1 cut(s) 774
NsiI ATGCAT 1 cut(s) 15
PceI AGGCCT 1 cut(s) 579
PctI GAATGC 4 cut(s) 13, 142, 382, 920
PfeI GAWTC 6 cut(s) 125, 431, 814, 941, 994, 1025
PinAI ACCGGT 1 cut(s) 977
PkrI GCNGC 6 cut(s) 199, 424, 511, 520, 674, 1042
PleI GAGTC 3 cut(s) 100, 167, 546
PpsI GAGTC 3 cut(s) 100, 167, 546
PpuMI RGGWCCY 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 452
PspN4I GGNNCC 1 cut(s) 774
PspPI GGNCC 2 cut(s) 452, 1034
PspPPI RGGWCCY 1 cut(s) 452
PsrI GAACNNNNNNTAC 2 cut(s) 692, 724
PstI CTGCAG 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 2 cut(s) 86, 772
PvuII CAGCTG 1 cut(s) 1040
RsaI GTAC 3 cut(s) 274, 289, 982
RsaNI GTAC 3 cut(s) 273, 288, 981
SaqAI TTAA 5 cut(s) 539, 894, 951, 1017, 1063
SatI GCNGC 6 cut(s) 198, 423, 510, 519, 673, 1041
Sau3AI GATC 7 cut(s) 86, 184, 251, 592, 688, 772, 847
Sau96I GGNCC 2 cut(s) 452, 1034
SchI GAGTC 3 cut(s) 100, 168, 546
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 616
SetI ASST 9 cut(s) 116, 454, 532, 574, 698, 710, 858, 1042, 1057
SfaNI GCATC 2 cut(s) 85, 787
SfcI CTRYAG 2 cut(s) 176, 573
SinI GGWCC 2 cut(s) 452, 1034
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 3 cut(s) 32, 255, 766
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 4 cut(s) 387, 672, 737, 870
SspI AATATT 2 cut(s) 60, 1061
SspMI CTAG 3 cut(s) 633, 761, 867
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 242, 356, 466
TaiI ACGT 1 cut(s) 116
TaqI TCGA 3 cut(s) 655, 812, 997
TaqII GACCGA 1 cut(s) 197
TasI AATT 3 cut(s) 32, 255, 766
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 6 cut(s) 125, 431, 814, 941, 994, 1025
Tru1I TTAA 5 cut(s) 539, 894, 951, 1017, 1063
Tru9I TTAA 5 cut(s) 539, 894, 951, 1017, 1063
TscAI CASTG 3 cut(s) 471, 595, 726
TseI GCWGC 5 cut(s) 197, 422, 509, 518, 1040
TspDTI ATGAA 4 cut(s) 17, 138, 447, 1017
TspRI CASTG 3 cut(s) 471, 595, 726
VpaK11BI GGWCC 2 cut(s) 452, 1034
XcmI CCANNNNNNNNNTGG 1 cut(s) 952
XspI CTAG 3 cut(s) 633, 761, 867
Zsp2I ATGCAT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.