MD03G1013300.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002716
Physical Location & Seq
Reverse (-)
1050737 .. 1053030
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1013300.v1.1.491

Sequence Viewer

Length: 951 bp
ATGCCTCCTTATAGTGCTCAATTGTATTACAAATATGTTTTGGCAGTGTTACTAATCTTGTGTGCAAGTGCAGGATGTGGTGATGCTCAGTTGACTCCTACGTTTTATGATGAATCATGCCCGAATGCCACCACCATTGTGCGTGGAGTCATTGAAGAGGCTCTGCAGACCGATCCCCGGATTACTGCCAGCCTCACTCGGCTTCACTTTCACGATTGCTTCGTCAACGGTTGCGATGGATCAATTCTGCTGGACAACAGTACCAATCCTAACAGTACCATTGACAGCGAAAAAACAGCAGTGCCAAATAACAACTCGGCCAGAGGATTTGATGTTGTGGACAATATCAAGACTGCATTGGAGAATGCTTGTCCCGCCGTCGTTTCCTGTGCTGATATTCTCGCCATTGCTGCCGAAGAATCTGTTGCTTTGTCTGGAGGTCCTTCATGGACAGTGCTATTAGGAAGAAGGGATAGCTTAACAGCTAATCGCACGGCTGCCAACGAAGCCCTTCCAGCTCCTACTTTTACACTTGACGAACTCGAGGCCAACTTCTTAGCTGTAGGCCTCAACACCACGGATCTGGTTGCACTATCTGGTGCTCACACATTTGGGCGTGCTAGATGTCTAACTTTCACCGATCGATTGTATAACTTCAGCGGCACAGGCAGTCCTGATCCGACCTTGAACTCAACCTACTTAGAAACATTGAGTGAGATATGCCCACTGAATGGGAATGGCAGTGTACTGACCAATTTGGATCCTGTAACTCCTGACACTTTCGATGCAAAGTATTTCTCGAATCTTCAAGTCGAGGAAGGCTTGCTCCAAAGCGATCAAGAGCTGTTTTCAACTAGCGGGGCTGATACCATCGACATTGTTAACAACTTCAGCGCTAATCAGAGCGCATTCTTTGAGAATTTCGGGGAATCCATGATTAAAAATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0020037 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0046906 GO:0048037 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097159 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901363 GO:1990748
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

317

Amino Acids

33.71

Weight (kDa)

4.19

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 47 - 299 1.2e-82 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 731
AciI CCGC 3 cut(s) 375, 660, 858
AclWI GGATC 6 cut(s) 167, 247, 588, 671, 755, 768
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 919
AcuI CTGAAG 2 cut(s) 640, 874
AfaI GTAC 3 cut(s) 262, 277, 747
AfeI AGCGCT 1 cut(s) 895
AfiI CCNNNNNNNGG 2 cut(s) 177, 731
AgsI TTSAA 4 cut(s) 155, 688, 809, 852
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 5 cut(s) 477, 485, 518, 560, 844
AluI AGCT 5 cut(s) 477, 485, 518, 560, 844
Alw21I GWGCWC 2 cut(s) 19, 604
AlwI GGATC 6 cut(s) 167, 247, 588, 671, 755, 768
Ama87I CYCGRG 1 cut(s) 542
Aor51HI AGCGCT 1 cut(s) 895
AoxI GGCC 3 cut(s) 318, 546, 565
ApeKI GCWGC 2 cut(s) 410, 497
ApoI RAATTY 1 cut(s) 919
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 2 cut(s) 896, 908
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 2 cut(s) 92, 628
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 1 cut(s) 440
BaeI ACNNNNGTAYC 2 cut(s) 244, 277
BamHI GGATCC 1 cut(s) 760
Bbv12I GWGCWC 2 cut(s) 19, 604
BbvI GCAGC 2 cut(s) 397, 484
BccI CCATC 2 cut(s) 230, 878
BceAI ACGGC 2 cut(s) 362, 510
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 2 cut(s) 621, 855
BfmI CTRYAG 2 cut(s) 164, 561
BfoI RGCGCY 1 cut(s) 897
BisI GCNGC 3 cut(s) 411, 498, 661
BlsI GCNGC 3 cut(s) 412, 499, 662
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 73, 775
BpmI CTGGAG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 643
BsaJI CCNNGG 2 cut(s) 176, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 731
Bse3DI GCAATG 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 643
BseDI CCNNGG 2 cut(s) 176, 576
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 2 cut(s) 177, 731
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 101
BseXI GCAGC 2 cut(s) 397, 484
BsgI GTGCAG 1 cut(s) 90
Bsh1285I CGRYCG 1 cut(s) 643
BshFI GGCC 3 cut(s) 320, 548, 567
BshVI ATCGAT 1 cut(s) 643
BsiEI CGRYCG 1 cut(s) 643
BsiHKAI GWGCWC 2 cut(s) 19, 604
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 177, 731
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 3 cut(s) 130, 370, 908
BsnI GGCC 3 cut(s) 320, 548, 567
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 19, 604
Bsp143I GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
BspACI CCGC 3 cut(s) 375, 660, 858
BspANI GGCC 3 cut(s) 320, 548, 567
BspCNI CTCAG 1 cut(s) 100
BspDI ATCGAT 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 6 cut(s) 167, 247, 588, 671, 755, 768
BsrDI GCAATG 1 cut(s) 405
BssECI CCNNGG 2 cut(s) 176, 576
BssMI GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
Bst4CI ACNGT 4 cut(s) 230, 260, 275, 454
Bst6I CTCTTC 1 cut(s) 150
BstC8I GCNNGC 3 cut(s) 190, 618, 824
BstDEI CTNAG 3 cut(s) 87, 556, 700
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 897
BstHHI GCGC 2 cut(s) 896, 908
BstKTI GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
BstMBI GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
BstMCI CGRYCG 1 cut(s) 643
BstMWI GCNNNNNNNGC 5 cut(s) 374, 410, 506, 515, 666
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 2 cut(s) 164, 561
BstV1I GCAGC 2 cut(s) 397, 484
BstX2I RGATCY 2 cut(s) 580, 760
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 2 cut(s) 580, 760
Bsu15I ATCGAT 1 cut(s) 643
BsuRI GGCC 3 cut(s) 320, 548, 567
BsuTUI ATCGAT 1 cut(s) 643
BtgI CCRYGG 1 cut(s) 576
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 3 cut(s) 51, 306, 748
BtsIMutI CAGTG 5 cut(s) 51, 306, 459, 725, 748
Cac8I GCNNGC 3 cut(s) 190, 618, 824
CfoI GCGC 2 cut(s) 896, 908
Cfr13I GGNCC 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 643
Csp6I GTAC 3 cut(s) 261, 276, 746
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 3 cut(s) 117, 447, 934
CviQI GTAC 3 cut(s) 261, 276, 746
DdeI CTNAG 3 cut(s) 87, 556, 700
DpnI GATC 7 cut(s) 174, 241, 582, 642, 678, 762, 837
DpnII GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 895
Eco57I CTGAAG 2 cut(s) 640, 874
Eco88I CYCGRG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 440
FaeI CATG 3 cut(s) 120, 450, 937
FaiI YATR 8 cut(s) 12, 36, 108, 118, 448, 651, 721, 935
FaqI GGGAC 1 cut(s) 357
FatI CATG 3 cut(s) 116, 446, 933
FauI CCCGC 2 cut(s) 382, 851
Fnu4HI GCNGC 3 cut(s) 411, 498, 661
FokI GGATG 1 cut(s) 87
Fsp4HI GCNGC 3 cut(s) 411, 498, 661
FspBI CTAG 2 cut(s) 621, 855
GlaI GCGC 2 cut(s) 895, 907
GluI GCNGC 3 cut(s) 411, 498, 661
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 897
HaeIII GGCC 3 cut(s) 320, 548, 567
HapII CCGG 1 cut(s) 178
HhaI GCGC 2 cut(s) 896, 908
Hin1II CATG 3 cut(s) 120, 450, 937
Hin6I GCGC 2 cut(s) 894, 906
HinP1I GCGC 2 cut(s) 894, 906
HincII GTYRAC 3 cut(s) 93, 226, 883
HindII GTYRAC 3 cut(s) 93, 226, 883
HinfI GANTC 6 cut(s) 94, 113, 147, 419, 802, 929
HpaI GTTAAC 1 cut(s) 883
HpaII CCGG 1 cut(s) 178
HphI GGTGA 2 cut(s) 92, 628
Hpy166II GTNNAC 5 cut(s) 93, 226, 340, 746, 883
Hpy188I TCNGA 2 cut(s) 681, 903
Hpy188III TCNNGA 7 cut(s) 212, 349, 435, 674, 773, 799, 839
Hpy8I GTNNAC 5 cut(s) 93, 226, 340, 746, 883
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 4 cut(s) 453, 462, 521, 812
HpyCH4III ACNGT 4 cut(s) 230, 260, 275, 454
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 6 cut(s) 65, 71, 166, 356, 590, 788
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 410, 506, 515, 666
HpyF3I CTNAG 3 cut(s) 87, 556, 700
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 3 cut(s) 120, 450, 937
HspAI GCGC 2 cut(s) 894, 906
KspAI GTTAAC 1 cut(s) 883
Kzo9I GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
LmnI GCTCC 2 cut(s) 523, 831
Lsp1109I GCAGC 2 cut(s) 397, 484
LweI GCATC 2 cut(s) 73, 775
MaeI CTAG 2 cut(s) 621, 855
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 48, 766
MalI GATC 7 cut(s) 174, 241, 582, 642, 678, 762, 837
MboI GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
MboII GAAGA 4 cut(s) 167, 428, 477, 797
MfeI CAATTG 1 cut(s) 20
MflI RGATCY 2 cut(s) 580, 760
MhlI GDGCHC 2 cut(s) 19, 604
MluCI AATT 4 cut(s) 20, 243, 754, 919
MlyI GAGTC 2 cut(s) 88, 156
MmeI TCCRAC 1 cut(s) 704
MnlI CCTC 8 cut(s) 15, 151, 203, 317, 431, 538, 578, 808
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 3 cut(s) 479, 882, 939
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 660
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 178
MunI CAATTG 1 cut(s) 20
Mva1269I GAATGC 3 cut(s) 130, 370, 908
MwoI GCNNNNNNNGC 5 cut(s) 374, 410, 506, 515, 666
NciI CCSGG 1 cut(s) 178
NdeII GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
NlaIII CATG 3 cut(s) 120, 450, 937
NlaIV GGNNCC 1 cut(s) 762
NmeAIII GCCGAG 2 cut(s) 178, 296
OliI CACNNNNGTG 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 542
PceI AGGCCT 1 cut(s) 567
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 3 cut(s) 130, 370, 908
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 4 cut(s) 113, 419, 802, 929
PflMI CCANNNNNTGG 1 cut(s) 731
PkrI GCNGC 3 cut(s) 412, 499, 662
Ple19I CGATCG 1 cut(s) 643
PleI GAGTC 2 cut(s) 88, 155
PpsI GAGTC 2 cut(s) 88, 155
PpuMI RGGWCCY 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 1 cut(s) 440
PspPPI RGGWCCY 1 cut(s) 440
PspXI VCTCGAGB 1 cut(s) 542
PsrI GAACNNNNNNTAC 2 cut(s) 680, 712
PstI CTGCAG 1 cut(s) 168
PsuI RGATCY 2 cut(s) 580, 760
PvuI CGATCG 1 cut(s) 643
RsaI GTAC 3 cut(s) 262, 277, 747
RsaNI GTAC 3 cut(s) 261, 276, 746
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 3 cut(s) 479, 882, 939
SatI GCNGC 3 cut(s) 411, 498, 661
Sau3AI GATC 7 cut(s) 172, 239, 580, 640, 676, 760, 835
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 2 cut(s) 88, 156
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 2 cut(s) 19, 604
SetI ASST 9 cut(s) 104, 442, 479, 487, 520, 562, 686, 698, 846
SfaNI GCATC 2 cut(s) 73, 775
SfcI CTRYAG 2 cut(s) 164, 561
Sfr274I CTCGAG 1 cut(s) 542
SinI GGWCC 1 cut(s) 440
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 4 cut(s) 20, 243, 754, 919
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 3 cut(s) 375, 660, 858
SspMI CTAG 2 cut(s) 621, 855
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 176
TaaI ACNGT 4 cut(s) 230, 260, 275, 454
TaiI ACGT 1 cut(s) 104
TaqI TCGA 6 cut(s) 543, 643, 783, 800, 813, 873
TaqII GACCGA 1 cut(s) 185
TasI AATT 4 cut(s) 20, 243, 754, 919
TatI WGTACW 1 cut(s) 745
TauI GCSGC 1 cut(s) 663
TfiI GAWTC 4 cut(s) 113, 419, 802, 929
Tru1I TTAA 3 cut(s) 479, 882, 939
Tru9I TTAA 3 cut(s) 479, 882, 939
TscAI CASTG 5 cut(s) 51, 306, 459, 732, 748
TseI GCWGC 2 cut(s) 410, 497
TspDTI ATGAA 2 cut(s) 126, 435
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 5 cut(s) 51, 306, 459, 732, 748
Van91I CCANNNNNTGG 1 cut(s) 731
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 919
XhoI CTCGAG 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 2 cut(s) 621, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.