MD03G1012800.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002716
Physical Location & Seq
Reverse (-)
978704 .. 981129
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1012800.v1.1.491

Sequence Viewer

Length: 903 bp
ATGCTCAAGGCTCTGCAGACCGATCCCCGGATTACTGCCAGCCTCACTCGGCTTCACTTTCACGATTGCTTCGTCAACGGTTGCGATGGATCAATTCTGCTGGACAACAGTACCAATCCTAACAGTACCATTGACAGCGAAAAAACAGCAGTGCCAAATAACAACTCGGCCAGAGGATTTGATGTTGTGGACAATATCAAGACTGCATTGGAGAATGCTTGTCCCGCCGTCGTTTCCTGTGCTGATATTCTCGCCATTGCTGCCGAAGAATCTGTTGCTTTGTCTGGAGGTCCTTCATGGACAGTGCTATTAGGAAGAAGGGATAGCTTAACAGCTAATCGCACGGCTGCCAACGAAGCCCTTCCAGCTCCTACTTTTACACTTGACGAACTCGAGGCCAACTTCTTAGCTGTAGGCCTCAACACCACGGATCTGGTTGCACTATCTGGTGCTCACACATTTGGGCGTGCTAGATGTCTAACTTTCACCGATCGATTGTATAACTTCAGCGGCACAGGCAGTCCTGATCCGACCTTGAACTCAACCTACTTAGAAACATTGAGTGAGATATGCCCACTGAATGGGAATGGCAGTGTACTGACCAATTTGGATCCTGTAACTCCTGACACTTTCGATGCAAAGTATTTCTCGAATCTTCAAGTCGAGGAAGGCTTGCTCCAAAGCGATCAAGAGCTGTTTTCAACTAGCGGGGCTGATACCATCGACATTGTTAACAACTTCAGCGCTAATCAGAGCGCATTCTTTGAGAATTTCGGGGAATCCATGATTAAAATGGGTAATATAAGCCCGTTGACCGGAACCGAGGGGGAGATTAGATTGAGCTGCCGTAGAGTTAATGGAGAATCATATGGATTAACTGCTACTTTGGTAGCTGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0020037 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0046906 GO:0048037 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097159 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901363 GO:1990748
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

301

Amino Acids

32.01

Weight (kDa)

4.32

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 3 - 249 2.3e-81 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 581
AciI CCGC 3 cut(s) 225, 510, 708
AclWI GGATC 6 cut(s) 17, 97, 438, 521, 605, 618
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 769
AcuI CTGAAG 2 cut(s) 490, 724
AfaI GTAC 4 cut(s) 112, 127, 597, 899
AfeI AGCGCT 1 cut(s) 745
AfiI CCNNNNNNNGG 3 cut(s) 27, 581, 815
AgsI TTSAA 3 cut(s) 538, 659, 702
AluBI AGCT 7 cut(s) 327, 335, 368, 410, 694, 843, 893
AluI AGCT 7 cut(s) 327, 335, 368, 410, 694, 843, 893
Alw21I GWGCWC 1 cut(s) 454
AlwI GGATC 6 cut(s) 17, 97, 438, 521, 605, 618
Ama87I CYCGRG 1 cut(s) 392
Aor51HI AGCGCT 1 cut(s) 745
AoxI GGCC 3 cut(s) 168, 396, 415
ApeKI GCWGC 3 cut(s) 260, 347, 843
ApoI RAATTY 1 cut(s) 769
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 2 cut(s) 746, 758
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 478
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 290
BaeI ACNNNNGTAYC 2 cut(s) 94, 127
BamHI GGATCC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 3 cut(s) 247, 334, 830
BccI CCATC 2 cut(s) 80, 728
BceAI ACGGC 3 cut(s) 212, 360, 831
BcnI CCSGG 1 cut(s) 28
BfaI CTAG 2 cut(s) 471, 705
BfmI CTRYAG 2 cut(s) 14, 411
BfoI RGCGCY 1 cut(s) 747
BisI GCNGC 4 cut(s) 261, 348, 511, 844
BlsI GCNGC 4 cut(s) 262, 349, 512, 845
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 1 cut(s) 28
Bme18I GGWCC 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 2 cut(s) 612, 820
BmrFI CCNGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 28
Bsa29I ATCGAT 1 cut(s) 493
BsaJI CCNNGG 3 cut(s) 26, 426, 822
BsaWI WCCGGW 1 cut(s) 815
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 581, 815
Bse3DI GCAATG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 3 cut(s) 26, 426, 822
BseLI CCNNNNNNNGG 3 cut(s) 27, 581, 815
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 885
BseXI GCAGC 3 cut(s) 247, 334, 830
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 3 cut(s) 170, 398, 417
BshVI ATCGAT 1 cut(s) 493
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 2 cut(s) 28, 816
BslFI GGGAC 1 cut(s) 207
BslI CCNNNNNNNGG 3 cut(s) 27, 581, 815
BsmFI GGGAC 1 cut(s) 207
BsmI GAATGC 2 cut(s) 220, 758
BsnI GGCC 3 cut(s) 170, 398, 417
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
BspACI CCGC 3 cut(s) 225, 510, 708
BspANI GGCC 3 cut(s) 170, 398, 417
BspCNI CTCAG 1 cut(s) 886
BspDI ATCGAT 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 612, 820
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 6 cut(s) 17, 97, 438, 521, 605, 618
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 3 cut(s) 26, 426, 822
BssMI GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
Bst4CI ACNGT 4 cut(s) 80, 110, 125, 304
BstC8I GCNNGC 3 cut(s) 40, 468, 674
BstDEI CTNAG 3 cut(s) 406, 550, 894
BstDSI CCRYGG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 747
BstHHI GCGC 2 cut(s) 746, 758
BstKTI GATC 7 cut(s) 25, 92, 433, 493, 529, 613, 688
BstMBI GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 5 cut(s) 224, 260, 356, 365, 516
BstSCI CCNGG 1 cut(s) 26
BstSFI CTRYAG 2 cut(s) 14, 411
BstV1I GCAGC 3 cut(s) 247, 334, 830
BstX2I RGATCY 2 cut(s) 430, 610
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 2 cut(s) 430, 610
Bsu15I ATCGAT 1 cut(s) 493
BsuRI GGCC 3 cut(s) 170, 398, 417
BsuTUI ATCGAT 1 cut(s) 493
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 99
BtsI GCAGTG 2 cut(s) 156, 598
BtsIMutI CAGTG 4 cut(s) 156, 309, 575, 598
Cac8I GCNNGC 3 cut(s) 40, 468, 674
CfoI GCGC 2 cut(s) 746, 758
Cfr13I GGNCC 1 cut(s) 290
ClaI ATCGAT 1 cut(s) 493
Csp6I GTAC 4 cut(s) 111, 126, 596, 898
CviAII CATG 2 cut(s) 297, 784
CviQI GTAC 4 cut(s) 111, 126, 596, 898
DdeI CTNAG 3 cut(s) 406, 550, 894
DpnI GATC 7 cut(s) 24, 91, 432, 492, 528, 612, 687
DpnII GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
EaeI YGGCCR 1 cut(s) 168
Eco147I AGGCCT 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 290
Eco47III AGCGCT 1 cut(s) 745
Eco57I CTGAAG 2 cut(s) 490, 724
Eco88I CYCGRG 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 290
FaeI CATG 2 cut(s) 300, 787
FaiI YATR 7 cut(s) 298, 501, 571, 785, 803, 868, 870
FaqI GGGAC 1 cut(s) 207
FatI CATG 2 cut(s) 296, 783
FauI CCCGC 2 cut(s) 232, 701
FauNDI CATATG 1 cut(s) 868
Fnu4HI GCNGC 4 cut(s) 261, 348, 511, 844
Fsp4HI GCNGC 4 cut(s) 261, 348, 511, 844
FspBI CTAG 2 cut(s) 471, 705
GlaI GCGC 2 cut(s) 745, 757
GluI GCNGC 4 cut(s) 261, 348, 511, 844
GsuI CTGGAG 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 747
HaeIII GGCC 3 cut(s) 170, 398, 417
HapII CCGG 2 cut(s) 28, 816
HhaI GCGC 2 cut(s) 746, 758
Hin1II CATG 2 cut(s) 300, 787
Hin6I GCGC 2 cut(s) 744, 756
HinP1I GCGC 2 cut(s) 744, 756
HincII GTYRAC 3 cut(s) 76, 733, 813
HindII GTYRAC 3 cut(s) 76, 733, 813
HinfI GANTC 4 cut(s) 269, 652, 779, 863
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 2 cut(s) 28, 816
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 5 cut(s) 76, 190, 596, 733, 813
Hpy188I TCNGA 2 cut(s) 531, 753
Hpy188III TCNNGA 7 cut(s) 62, 199, 285, 524, 623, 649, 689
Hpy8I GTNNAC 5 cut(s) 76, 190, 596, 733, 813
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 303, 312, 371, 662
HpyCH4III ACNGT 4 cut(s) 80, 110, 125, 304
HpyCH4V TGCA 4 cut(s) 16, 206, 440, 638
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 260, 356, 365, 516
HpyF3I CTNAG 3 cut(s) 406, 550, 894
Hsp92II CATG 2 cut(s) 300, 787
HspAI GCGC 2 cut(s) 744, 756
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
LmnI GCTCC 2 cut(s) 373, 681
Lsp1109I GCAGC 3 cut(s) 247, 334, 830
LweI GCATC 1 cut(s) 625
MaeI CTAG 2 cut(s) 471, 705
MaeIII GTNAC 1 cut(s) 616
MalI GATC 7 cut(s) 24, 91, 432, 492, 528, 612, 687
MboI GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
MboII GAAGA 3 cut(s) 278, 327, 647
MflI RGATCY 2 cut(s) 430, 610
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 3 cut(s) 93, 604, 769
MmeI TCCRAC 1 cut(s) 554
MnlI CCTC 7 cut(s) 53, 167, 281, 388, 428, 658, 817
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 5 cut(s) 329, 732, 789, 855, 875
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 2 cut(s) 28, 816
MspR9I CCNGG 1 cut(s) 28
Mva1269I GAATGC 2 cut(s) 220, 758
MwoI GCNNNNNNNGC 5 cut(s) 224, 260, 356, 365, 516
NciI CCSGG 1 cut(s) 28
NdeI CATATG 1 cut(s) 868
NdeII GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
NlaIII CATG 2 cut(s) 300, 787
NlaIV GGNNCC 2 cut(s) 612, 820
NmeAIII GCCGAG 2 cut(s) 28, 146
PaeR7I CTCGAG 1 cut(s) 392
PceI AGGCCT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PctI GAATGC 2 cut(s) 220, 758
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 4 cut(s) 269, 652, 779, 863
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 4 cut(s) 262, 349, 512, 845
Ple19I CGATCG 1 cut(s) 493
PpuMI RGGWCCY 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 290
PspN4I GGNNCC 2 cut(s) 612, 820
PspPI GGNCC 1 cut(s) 290
PspPPI RGGWCCY 1 cut(s) 290
PspXI VCTCGAGB 1 cut(s) 392
PsrI GAACNNNNNNTAC 2 cut(s) 530, 562
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 2 cut(s) 430, 610
PvuI CGATCG 1 cut(s) 493
RsaI GTAC 4 cut(s) 112, 127, 597, 899
RsaNI GTAC 4 cut(s) 111, 126, 596, 898
SaqAI TTAA 5 cut(s) 329, 732, 789, 855, 875
SatI GCNGC 4 cut(s) 261, 348, 511, 844
Sau3AI GATC 7 cut(s) 22, 89, 430, 490, 526, 610, 685
Sau96I GGNCC 1 cut(s) 290
ScaI AGTACT 1 cut(s) 899
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 454
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 2 cut(s) 14, 411
Sfr274I CTCGAG 1 cut(s) 392
SinI GGWCC 1 cut(s) 290
SlaI CTCGAG 1 cut(s) 392
SmlI CTYRAG 2 cut(s) 5, 392
SmoI CTYRAG 2 cut(s) 5, 392
Sse9I AATT 3 cut(s) 93, 604, 769
SseBI AGGCCT 1 cut(s) 417
SsiI CCGC 3 cut(s) 225, 510, 708
SspMI CTAG 2 cut(s) 471, 705
StuI AGGCCT 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 26
TaaI ACNGT 4 cut(s) 80, 110, 125, 304
TaqI TCGA 6 cut(s) 393, 493, 633, 650, 663, 723
TaqII GACCGA 1 cut(s) 35
TasI AATT 3 cut(s) 93, 604, 769
TatI WGTACW 2 cut(s) 595, 897
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 4 cut(s) 269, 652, 779, 863
Tru1I TTAA 5 cut(s) 329, 732, 789, 855, 875
Tru9I TTAA 5 cut(s) 329, 732, 789, 855, 875
TscAI CASTG 4 cut(s) 156, 309, 582, 598
TseI GCWGC 3 cut(s) 260, 347, 843
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 1 cut(s) 443
TspRI CASTG 4 cut(s) 156, 309, 582, 598
Van91I CCANNNNNTGG 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 1 cut(s) 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XhoI CTCGAG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 2 cut(s) 471, 705
ZrmI AGTACT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.