MD03G1013600.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002716
Physical Location & Seq
Reverse (-)
1071235 .. 1073468
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1013600.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGCCTTCTTCTTGTAGCGCTCAATTTTGTTACAAATATATTTTTGTAATCTTACTAATCTTGTGTGCAAGTGCAGGATGTGGTGATGCTCAGTTGACTCCTACGTTTTATGATGCATCATGCCCGAATGCCACCAGCATTGTGCGTGGAGTCATTCAAGAGGCTCTGCAGACCGATCCCCGGATTGCTGCCAGCCTCATTCGACTTCACTTCCACGATTGCTTCGTCAACGGTTGCGATGGATCAATTCTGTTGGACAATAGTACCAGTTCTACCAGTACCATAGACAGCGAAAAAACAGCATTTGCAAATAACAACTCAGCCAGAGGATTTGATGTTGTGGACAGTATCAAGACTGCATTGGAGAATGCTTGTCCCGCCATCGTTTCCTGTGCTGATATTCTCACCATTGCTGCCGAAGAATCTGTTGCTTTGTCTGGAGGTCCTTCATGGACAGTGCTACTGGGAAGAAGGGATAGCACAACAGCGAACCGCACGGCTGCCAACGCAGCCATTCCAGCTCCTACTTTAACACTTGATGGACTCAAGGCCAACTTCTTAGCTGTAGGCCTCAACACCACTGATCTGGTTGCACTATCTGGTGCTCACACATTTGGGCGTGCTAGATGTCAATCTTTCACCAATCGATTGTATAACTTCAGCGGCACAGGCAGTCCTGATCCAACCTTGAACTCAACCTACTTACAAACACTGAGTGAGATATGCCCGCAGAATGGGAATAGCAGTGTGCTAGCCAATTTGGATCCTGTAACGCCCGACACTTTTGATGCAAAGTATTTCTCGAATCTTCAAGTCCAGAAAGGCTTGCTCCAAAGCGATCAAGAGCTGTTTTCAACTAGCGGGGCTGATACCACCGACATTGTTAACAACTTCAGCACTAATCAGAGCGCATTCTTTGAGAGTTTCGTGGAATCCATGATTAAAATGGGGAATATAAGCCCCTTGACCGGTACTGATGGAGAGATTCGATTGAACTGCCGTAGGGTTAATGGAGATTCATATGGACCAGCTGCTACTTTGGTAGCTGAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0020037 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0046906 GO:0048037 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097159 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901363 GO:1990748
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

352

Amino Acids

37.18

Weight (kDa)

4.56

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 300 1.8e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 378, 493, 663, 728, 861
AclWI GGATC 5 cut(s) 170, 250, 674, 758, 771
AcuI CTGAAG 2 cut(s) 643, 877
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 3 cut(s) 265, 280, 973
AfeI AGCGCT 1 cut(s) 19
AfiI CCNNNNNNNGG 3 cut(s) 180, 734, 968
AgeI ACCGGT 1 cut(s) 968
AgsI TTSAA 5 cut(s) 158, 691, 812, 855, 994
AluBI AGCT 5 cut(s) 521, 563, 847, 1031, 1046
AluI AGCT 5 cut(s) 521, 563, 847, 1031, 1046
Alw21I GWGCWC 1 cut(s) 607
AlwI GGATC 5 cut(s) 170, 250, 674, 758, 771
Aor51HI AGCGCT 1 cut(s) 19
AoxI GGCC 2 cut(s) 549, 568
ApeKI GCWGC 5 cut(s) 188, 413, 500, 509, 1031
AsiGI ACCGGT 1 cut(s) 968
AspLEI GCGC 2 cut(s) 20, 911
AspS9I GGNCC 2 cut(s) 443, 1025
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 3 cut(s) 95, 397, 631
AsuNHI GCTAGC 1 cut(s) 751
AvaII GGWCC 2 cut(s) 443, 1025
BaeI ACNNNNGTAYC 2 cut(s) 247, 280
BamHI GGATCC 1 cut(s) 763
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 5 cut(s) 175, 400, 487, 521, 1018
BccI CCATC 4 cut(s) 233, 389, 533, 971
BceAI ACGGC 2 cut(s) 513, 984
BcgI CGANNNNNNTGC 2 cut(s) 978, 1012
BcnI CCSGG 1 cut(s) 181
BfaI CTAG 3 cut(s) 624, 752, 858
BfmI CTRYAG 2 cut(s) 167, 564
BfoI RGCGCY 1 cut(s) 21
BisI GCNGC 6 cut(s) 189, 414, 501, 510, 664, 1032
BlsI GCNGC 6 cut(s) 190, 415, 502, 511, 665, 1033
Bme1390I CCNGG 1 cut(s) 181
Bme18I GGWCC 2 cut(s) 443, 1025
BmgT120I GGNCC 2 cut(s) 443, 1025
BmiI GGNNCC 1 cut(s) 765
BmrFI CCNGG 1 cut(s) 181
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 4 cut(s) 76, 103, 125, 778
BmtI GCTAGC 1 cut(s) 755
BmuI ACTGGG 1 cut(s) 473
BpmI CTGGAG 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 631
BsaJI CCNNGG 1 cut(s) 179
BsaWI WCCGGW 1 cut(s) 968
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 734, 968
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 3 cut(s) 267, 276, 468
Bse3DI GCAATG 1 cut(s) 408
Bse8I GATNNNNATC 1 cut(s) 631
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 1 cut(s) 83
BseJI GATNNNNATC 1 cut(s) 631
BseLI CCNNNNNNNGG 3 cut(s) 180, 734, 968
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 104, 333, 704
BseNI ACTGG 3 cut(s) 267, 276, 468
BseXI GCAGC 5 cut(s) 175, 400, 487, 521, 1018
BsgI GTGCAG 1 cut(s) 93
BshFI GGCC 2 cut(s) 551, 570
BshTI ACCGGT 1 cut(s) 968
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 607
BsiSI CCGG 2 cut(s) 181, 969
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 3 cut(s) 180, 734, 968
BsmFI GGGAC 1 cut(s) 360
BsmI GAATGC 3 cut(s) 133, 373, 911
BsnI GGCC 2 cut(s) 551, 570
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 6 cut(s) 175, 242, 583, 679, 763, 838
BspACI CCGC 5 cut(s) 378, 493, 663, 728, 861
BspANI GGCC 2 cut(s) 551, 570
BspCNI CTCAG 3 cut(s) 103, 332, 705
BspDI ATCGAT 1 cut(s) 646
BspLI GGNNCC 1 cut(s) 765
BspMAI CTGCAG 1 cut(s) 171
BspOI GCTAGC 1 cut(s) 755
BspPI GGATC 5 cut(s) 170, 250, 674, 758, 771
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 968
BsrI ACTGG 3 cut(s) 267, 276, 468
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 6 cut(s) 175, 242, 583, 679, 763, 838
Bst4CI ACNGT 3 cut(s) 233, 347, 457
BstC8I GCNNGC 5 cut(s) 193, 621, 728, 753, 827
BstDEI CTNAG 4 cut(s) 90, 319, 559, 713
BstF5I GGATG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 2 cut(s) 20, 911
BstKTI GATC 6 cut(s) 178, 245, 586, 682, 766, 841
BstMBI GATC 6 cut(s) 175, 242, 583, 679, 763, 838
BstMWI GCNNNNNNNGC 5 cut(s) 377, 506, 509, 518, 669
BstSCI CCNGG 1 cut(s) 179
BstSFI CTRYAG 2 cut(s) 167, 564
BstV1I GCAGC 5 cut(s) 175, 400, 487, 521, 1018
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 646
BsuRI GGCC 2 cut(s) 551, 570
BsuTUI ATCGAT 1 cut(s) 646
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 83
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 4 cut(s) 462, 579, 710, 751
Cac8I GCNNGC 5 cut(s) 193, 621, 728, 753, 827
CfoI GCGC 2 cut(s) 20, 911
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 2 cut(s) 443, 1025
ClaI ATCGAT 1 cut(s) 646
Csp6I GTAC 3 cut(s) 264, 279, 972
CspAI ACCGGT 1 cut(s) 968
CspCI CAANNNNNGTGG 4 cut(s) 121, 156, 862, 897
CviAII CATG 3 cut(s) 120, 450, 937
CviQI GTAC 3 cut(s) 264, 279, 972
DdeI CTNAG 4 cut(s) 90, 319, 559, 713
DpnI GATC 6 cut(s) 177, 244, 585, 681, 765, 840
DpnII GATC 6 cut(s) 175, 242, 583, 679, 763, 838
DraIII CACNNNGTG 1 cut(s) 716
Eco147I AGGCCT 1 cut(s) 570
Eco47I GGWCC 2 cut(s) 443, 1025
Eco47III AGCGCT 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 643, 877
EcoO109I RGGNCCY 1 cut(s) 443
EcoT22I ATGCAT 1 cut(s) 118
FaeI CATG 3 cut(s) 123, 453, 940
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 1 cut(s) 360
FatI CATG 3 cut(s) 119, 449, 936
FauI CCCGC 3 cut(s) 385, 735, 854
FauNDI CATATG 1 cut(s) 1021
Fnu4HI GCNGC 6 cut(s) 189, 414, 501, 510, 664, 1032
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 6 cut(s) 189, 414, 501, 510, 664, 1032
FspBI CTAG 3 cut(s) 624, 752, 858
GlaI GCGC 2 cut(s) 19, 910
GluI GCNGC 6 cut(s) 189, 414, 501, 510, 664, 1032
GsuI CTGGAG 1 cut(s) 459
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 2 cut(s) 551, 570
HapII CCGG 2 cut(s) 181, 969
HhaI GCGC 2 cut(s) 20, 911
Hin1II CATG 3 cut(s) 123, 453, 940
Hin6I GCGC 2 cut(s) 18, 909
HinP1I GCGC 2 cut(s) 18, 909
HincII GTYRAC 3 cut(s) 96, 229, 886
HindII GTYRAC 3 cut(s) 96, 229, 886
HinfI GANTC 8 cut(s) 97, 150, 422, 543, 805, 932, 985, 1016
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 2 cut(s) 181, 969
HphI GGTGA 3 cut(s) 95, 397, 631
Hpy166II GTNNAC 4 cut(s) 96, 229, 343, 886
Hpy188I TCNGA 1 cut(s) 906
Hpy188III TCNNGA 7 cut(s) 158, 352, 438, 677, 802, 817, 842
Hpy8I GTNNAC 4 cut(s) 96, 229, 343, 886
HpyAV CCTTC 3 cut(s) 15, 456, 465
HpyCH4III ACNGT 3 cut(s) 233, 347, 457
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 8 cut(s) 68, 74, 116, 169, 308, 359, 593, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 377, 506, 509, 518, 669
HpyF3I CTNAG 4 cut(s) 90, 319, 559, 713
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 3 cut(s) 123, 453, 940
HspAI GCGC 2 cut(s) 18, 909
KspAI GTTAAC 1 cut(s) 886
Kzo9I GATC 6 cut(s) 175, 242, 583, 679, 763, 838
LmnI GCTCC 2 cut(s) 526, 834
Lsp1109I GCAGC 5 cut(s) 175, 400, 487, 521, 1018
LweI GCATC 4 cut(s) 76, 103, 125, 778
MaeI CTAG 3 cut(s) 624, 752, 858
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 2 cut(s) 29, 769
MalI GATC 6 cut(s) 177, 244, 585, 681, 765, 840
MboI GATC 6 cut(s) 175, 242, 583, 679, 763, 838
MboII GAAGA 3 cut(s) 431, 480, 800
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 3 cut(s) 23, 246, 757
MlyI GAGTC 3 cut(s) 91, 159, 537
MmeI TCCRAC 2 cut(s) 234, 707
MnlI CCTC 5 cut(s) 154, 206, 320, 434, 581
Mph1103I ATGCAT 1 cut(s) 118
MseI TTAA 5 cut(s) 530, 885, 942, 1008, 1054
MspA1I CMGCKG 2 cut(s) 663, 1031
MspI CCGG 2 cut(s) 181, 969
MspR9I CCNGG 1 cut(s) 181
Mva1269I GAATGC 3 cut(s) 133, 373, 911
MwoI GCNNNNNNNGC 5 cut(s) 377, 506, 509, 518, 669
NciI CCSGG 1 cut(s) 181
NdeI CATATG 1 cut(s) 1021
NdeII GATC 6 cut(s) 175, 242, 583, 679, 763, 838
NheI GCTAGC 1 cut(s) 751
NlaIII CATG 3 cut(s) 123, 453, 940
NlaIV GGNNCC 1 cut(s) 765
NsiI ATGCAT 1 cut(s) 118
PceI AGGCCT 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 3 cut(s) 133, 373, 911
PfeI GAWTC 5 cut(s) 422, 805, 932, 985, 1016
PinAI ACCGGT 1 cut(s) 968
PkrI GCNGC 6 cut(s) 190, 415, 502, 511, 665, 1033
PleI GAGTC 3 cut(s) 91, 158, 537
PpsI GAGTC 3 cut(s) 91, 158, 537
PpuMI RGGWCCY 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 443
PspN4I GGNNCC 1 cut(s) 765
PspPI GGNCC 2 cut(s) 443, 1025
PspPPI RGGWCCY 1 cut(s) 443
PsrI GAACNNNNNNTAC 2 cut(s) 683, 715
PstI CTGCAG 1 cut(s) 171
PsuI RGATCY 1 cut(s) 763
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 3 cut(s) 265, 280, 973
RsaNI GTAC 3 cut(s) 264, 279, 972
SaqAI TTAA 5 cut(s) 530, 885, 942, 1008, 1054
SatI GCNGC 6 cut(s) 189, 414, 501, 510, 664, 1032
Sau3AI GATC 6 cut(s) 175, 242, 583, 679, 763, 838
Sau96I GGNCC 2 cut(s) 443, 1025
SchI GAGTC 3 cut(s) 91, 159, 537
ScrFI CCNGG 1 cut(s) 181
SduI GDGCHC 1 cut(s) 607
SetI ASST 9 cut(s) 107, 445, 523, 565, 689, 701, 849, 1033, 1048
SfaNI GCATC 4 cut(s) 76, 103, 125, 778
SfcI CTRYAG 2 cut(s) 167, 564
SinI GGWCC 2 cut(s) 443, 1025
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 3 cut(s) 23, 246, 757
SseBI AGGCCT 1 cut(s) 570
SsiI CCGC 5 cut(s) 378, 493, 663, 728, 861
SspI AATATT 1 cut(s) 1052
SspMI CTAG 3 cut(s) 624, 752, 858
StuI AGGCCT 1 cut(s) 570
StyD4I CCNGG 1 cut(s) 179
TaaI ACNGT 3 cut(s) 233, 347, 457
TaiI ACGT 1 cut(s) 107
TaqI TCGA 4 cut(s) 202, 646, 803, 988
TaqII GACCGA 1 cut(s) 188
TasI AATT 3 cut(s) 23, 246, 757
TauI GCSGC 1 cut(s) 666
TfiI GAWTC 5 cut(s) 422, 805, 932, 985, 1016
Tru1I TTAA 5 cut(s) 530, 885, 942, 1008, 1054
Tru9I TTAA 5 cut(s) 530, 885, 942, 1008, 1054
TscAI CASTG 4 cut(s) 462, 586, 717, 751
TseI GCWGC 5 cut(s) 188, 413, 500, 509, 1031
TspDTI ATGAA 2 cut(s) 438, 1008
TspRI CASTG 4 cut(s) 462, 586, 717, 751
VpaK11BI GGWCC 2 cut(s) 443, 1025
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XspI CTAG 3 cut(s) 624, 752, 858
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.