MD03G1012900.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002716
Physical Location & Seq
Reverse (-)
1007836 .. 1010169
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1012900.v1.1.491

Sequence Viewer

Length: 930 bp
ATGCCTCCTTATAGTGCTCAATTGTATTACAAATATGTTTTGGCAGTGTTACTAATCTTGTGTGCAAGTGCAGGATGTGGTGATGCTCAGTTGACTCCTACGTTTTATGATGAATCATGCCCGAATGCCACCACCATTGTGCGTGGAGTCATTGAAGAGGCTCTGCAGACCGATCCCCGGATTACTGCCAGCCTCACTCGGCTTCACTTTCACGATTGCTTCGTCAACGGTTGCGATGGATCAATTCTGCTGGACAACAGTACCAATCCTAACAGTACCATTGACAGCGAAAAAACAGCAGTGCCAAATAACAACTCGGCCAGAGGATTTGATGTTGTGGACAATATCAAGACTGCATTGGAGAATGCTTGTCCCGCCGTCGTTTCCTGTGCTGATATTCTCGCCATTGCTGCCGAAGAATCTGTTGCTTTGTCTGGAGGTCCTTCATGGACAGTGCTATTAGGAAGAAGGGATAGCTTAACAGCTAATCGCACGGCTGCCAACGAAGCCCTTCCAGCTCCTACTTTTACACTTGACGAACTCGAGGCCAACTTCTTAGCTGTAGGCCTCAACACCACGGATCTGGTTTGCACTATCTGCGGCACAGGCAGTCCTGATCCGACCTTGAACTCAACCTACTTAGAAACATTGAGTGAGATATGCCCACTGAATGGGAATGGCAGTGTACTGACCAATTTGGATCCTGTAACTCCTGACACTTTCGATGCAAAGTATTTCTCGAATCTTCAAGTCGAGGAAGGCTTGCTCCAAAGCGATCAAGAGCTGTTTTCAACTAGCGGGGCTGATACCATCGACATTGTTAACAACTTCAGCGCTAATCAGAGCGCATTCTTTGAGAATTTCGGGGAATCCATGATTAAAATGGGTAATATAAGCCCGTTGACCGGAACCGAGGGGAGATTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0020037 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0046906 GO:0048037 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097159 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901363 GO:1990748
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

310

Amino Acids

32.82

Weight (kDa)

4.06

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 47 - 279 7.7e-66 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 671
AciI CCGC 3 cut(s) 375, 600, 798
AclWI GGATC 6 cut(s) 167, 247, 588, 611, 695, 708
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 859
AcuI CTGAAG 1 cut(s) 814
AfaI GTAC 3 cut(s) 262, 277, 687
AfeI AGCGCT 1 cut(s) 835
AfiI CCNNNNNNNGG 3 cut(s) 177, 671, 905
AgsI TTSAA 4 cut(s) 155, 628, 749, 792
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 5 cut(s) 477, 485, 518, 560, 784
AluI AGCT 5 cut(s) 477, 485, 518, 560, 784
Alw21I GWGCWC 1 cut(s) 19
AlwI GGATC 6 cut(s) 167, 247, 588, 611, 695, 708
Ama87I CYCGRG 1 cut(s) 542
Aor51HI AGCGCT 1 cut(s) 835
AoxI GGCC 3 cut(s) 318, 546, 565
ApeKI GCWGC 2 cut(s) 410, 497
ApoI RAATTY 1 cut(s) 859
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 2 cut(s) 836, 848
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 1 cut(s) 440
BaeI ACNNNNGTAYC 2 cut(s) 244, 277
BamHI GGATCC 1 cut(s) 700
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 397, 484
BccI CCATC 2 cut(s) 230, 818
BceAI ACGGC 2 cut(s) 362, 510
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 2 cut(s) 164, 561
BfoI RGCGCY 1 cut(s) 837
BisI GCNGC 3 cut(s) 411, 498, 601
BlsI GCNGC 3 cut(s) 412, 499, 602
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 702, 910
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 73, 715
BpmI CTGGAG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 176, 576, 912
BsaWI WCCGGW 1 cut(s) 905
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 671, 905
Bse3DI GCAATG 1 cut(s) 405
BseDI CCNNGG 3 cut(s) 176, 576, 912
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 3 cut(s) 177, 671, 905
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 101
BseXI GCAGC 2 cut(s) 397, 484
BsgI GTGCAG 1 cut(s) 90
BshFI GGCC 3 cut(s) 320, 548, 567
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 2 cut(s) 178, 906
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 3 cut(s) 177, 671, 905
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 3 cut(s) 130, 370, 848
BsnI GGCC 3 cut(s) 320, 548, 567
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 6 cut(s) 172, 239, 580, 616, 700, 775
BspACI CCGC 3 cut(s) 375, 600, 798
BspANI GGCC 3 cut(s) 320, 548, 567
BspCNI CTCAG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 702, 910
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 6 cut(s) 167, 247, 588, 611, 695, 708
BsrDI GCAATG 1 cut(s) 405
BssECI CCNNGG 3 cut(s) 176, 576, 912
BssMI GATC 6 cut(s) 172, 239, 580, 616, 700, 775
Bst4CI ACNGT 4 cut(s) 230, 260, 275, 454
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 190, 764
BstDEI CTNAG 3 cut(s) 87, 556, 640
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 837
BstHHI GCGC 2 cut(s) 836, 848
BstKTI GATC 6 cut(s) 175, 242, 583, 619, 703, 778
BstMBI GATC 6 cut(s) 172, 239, 580, 616, 700, 775
BstMWI GCNNNNNNNGC 6 cut(s) 374, 410, 506, 515, 597, 606
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 2 cut(s) 164, 561
BstV1I GCAGC 2 cut(s) 397, 484
BstX2I RGATCY 2 cut(s) 580, 700
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 2 cut(s) 580, 700
BsuRI GGCC 3 cut(s) 320, 548, 567
BtgI CCRYGG 1 cut(s) 576
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 3 cut(s) 51, 306, 688
BtsIMutI CAGTG 5 cut(s) 51, 306, 459, 665, 688
Cac8I GCNNGC 2 cut(s) 190, 764
CfoI GCGC 2 cut(s) 836, 848
Cfr13I GGNCC 1 cut(s) 440
Csp6I GTAC 3 cut(s) 261, 276, 686
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 3 cut(s) 117, 447, 874
CviQI GTAC 3 cut(s) 261, 276, 686
DdeI CTNAG 3 cut(s) 87, 556, 640
DpnI GATC 6 cut(s) 174, 241, 582, 618, 702, 777
DpnII GATC 6 cut(s) 172, 239, 580, 616, 700, 775
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 814
Eco88I CYCGRG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 440
FaeI CATG 3 cut(s) 120, 450, 877
FaiI YATR 8 cut(s) 12, 36, 108, 118, 448, 661, 875, 893
FaqI GGGAC 1 cut(s) 357
FatI CATG 3 cut(s) 116, 446, 873
FauI CCCGC 2 cut(s) 382, 791
Fnu4HI GCNGC 3 cut(s) 411, 498, 601
FokI GGATG 1 cut(s) 87
Fsp4HI GCNGC 3 cut(s) 411, 498, 601
FspBI CTAG 1 cut(s) 795
GlaI GCGC 2 cut(s) 835, 847
GluI GCNGC 3 cut(s) 411, 498, 601
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 837
HaeIII GGCC 3 cut(s) 320, 548, 567
HapII CCGG 2 cut(s) 178, 906
HhaI GCGC 2 cut(s) 836, 848
Hin1II CATG 3 cut(s) 120, 450, 877
Hin6I GCGC 2 cut(s) 834, 846
HinP1I GCGC 2 cut(s) 834, 846
HincII GTYRAC 4 cut(s) 93, 226, 823, 903
HindII GTYRAC 4 cut(s) 93, 226, 823, 903
HinfI GANTC 6 cut(s) 94, 113, 147, 419, 742, 869
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 2 cut(s) 178, 906
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 6 cut(s) 93, 226, 340, 686, 823, 903
Hpy188I TCNGA 2 cut(s) 621, 843
Hpy188III TCNNGA 7 cut(s) 212, 349, 435, 614, 713, 739, 779
Hpy8I GTNNAC 6 cut(s) 93, 226, 340, 686, 823, 903
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 4 cut(s) 453, 462, 521, 752
HpyCH4III ACNGT 4 cut(s) 230, 260, 275, 454
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 6 cut(s) 65, 71, 166, 356, 591, 728
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 410, 506, 515, 597, 606
HpyF3I CTNAG 3 cut(s) 87, 556, 640
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 3 cut(s) 120, 450, 877
HspAI GCGC 2 cut(s) 834, 846
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 6 cut(s) 172, 239, 580, 616, 700, 775
LmnI GCTCC 2 cut(s) 523, 771
Lsp1109I GCAGC 2 cut(s) 397, 484
LweI GCATC 2 cut(s) 73, 715
MaeI CTAG 1 cut(s) 795
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 48, 706
MalI GATC 6 cut(s) 174, 241, 582, 618, 702, 777
MboI GATC 6 cut(s) 172, 239, 580, 616, 700, 775
MboII GAAGA 4 cut(s) 167, 428, 477, 737
MfeI CAATTG 1 cut(s) 20
MflI RGATCY 2 cut(s) 580, 700
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 4 cut(s) 20, 243, 694, 859
MlyI GAGTC 2 cut(s) 88, 156
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 9 cut(s) 15, 151, 203, 317, 431, 538, 578, 748, 907
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 3 cut(s) 479, 822, 879
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 2 cut(s) 178, 906
MspR9I CCNGG 1 cut(s) 178
MunI CAATTG 1 cut(s) 20
Mva1269I GAATGC 3 cut(s) 130, 370, 848
MwoI GCNNNNNNNGC 6 cut(s) 374, 410, 506, 515, 597, 606
NciI CCSGG 1 cut(s) 178
NdeII GATC 6 cut(s) 172, 239, 580, 616, 700, 775
NlaIII CATG 3 cut(s) 120, 450, 877
NlaIV GGNNCC 2 cut(s) 702, 910
NmeAIII GCCGAG 2 cut(s) 178, 296
OliI CACNNNNGTG 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 542
PceI AGGCCT 1 cut(s) 567
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 3 cut(s) 130, 370, 848
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 4 cut(s) 113, 419, 742, 869
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 3 cut(s) 412, 499, 602
PleI GAGTC 2 cut(s) 88, 155
PpsI GAGTC 2 cut(s) 88, 155
PpuMI RGGWCCY 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 440
PspN4I GGNNCC 2 cut(s) 702, 910
PspPI GGNCC 1 cut(s) 440
PspPPI RGGWCCY 1 cut(s) 440
PspXI VCTCGAGB 1 cut(s) 542
PsrI GAACNNNNNNTAC 2 cut(s) 620, 652
PstI CTGCAG 1 cut(s) 168
PsuI RGATCY 2 cut(s) 580, 700
RsaI GTAC 3 cut(s) 262, 277, 687
RsaNI GTAC 3 cut(s) 261, 276, 686
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 3 cut(s) 479, 822, 879
SatI GCNGC 3 cut(s) 411, 498, 601
Sau3AI GATC 6 cut(s) 172, 239, 580, 616, 700, 775
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 2 cut(s) 88, 156
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 1 cut(s) 19
SetI ASST 9 cut(s) 104, 442, 479, 487, 520, 562, 626, 638, 786
SfaNI GCATC 2 cut(s) 73, 715
SfcI CTRYAG 2 cut(s) 164, 561
Sfr274I CTCGAG 1 cut(s) 542
SinI GGWCC 1 cut(s) 440
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 4 cut(s) 20, 243, 694, 859
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 3 cut(s) 375, 600, 798
SspMI CTAG 1 cut(s) 795
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 176
TaaI ACNGT 4 cut(s) 230, 260, 275, 454
TaiI ACGT 1 cut(s) 104
TaqI TCGA 5 cut(s) 543, 723, 740, 753, 813
TaqII GACCGA 1 cut(s) 185
TasI AATT 4 cut(s) 20, 243, 694, 859
TatI WGTACW 1 cut(s) 685
TauI GCSGC 1 cut(s) 603
TfiI GAWTC 4 cut(s) 113, 419, 742, 869
Tru1I TTAA 3 cut(s) 479, 822, 879
Tru9I TTAA 3 cut(s) 479, 822, 879
TscAI CASTG 5 cut(s) 51, 306, 459, 672, 688
TseI GCWGC 2 cut(s) 410, 497
TspDTI ATGAA 2 cut(s) 126, 435
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 5 cut(s) 51, 306, 459, 672, 688
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XhoI CTCGAG 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.