MD03G1013700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002716
Physical Location & Seq
Reverse (-)
1084725 .. 1087084
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1013700.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGCCTCCTTCTTATAGTGCTCAATTGTATTACAAATATGTTTTGGCAGTGTTACTAATCTTGTGTGCAAGTGCAGGATGTGGTGATGCTCAGTTGACTCCTACGTTTTATGATGAAACATGCCCGAATGCCACCAGCATTGTGCGTGGAGTCATTGAAGAGGCTCTGCAGACCGATCCCCGGATTACTGCCAGCCTCACTCGGCTTCACTTTCACGATTGCTTTGTCAACGGTTGCGATGGATCAATTCTGCTGGACAACAGTACCAATCCTAACAGTACCATTGACAGCGAAAAAACAGCATTGGCAAATAACAACTCGGCCAGAGGATTTGATGTTGTGGACAATATCAAGACTGCATTGGAGAATGCTTGTCCCGCCGTCGTTTCCTGTGCTGATATTCTCGCCATTGCTGCCGAAGAATCTGTTGCTTTGTCTGGAGGTCCTTCATGGACAGTGCTATTAGGAAGAAGGGATAGCTTAACAGCTAATCGCACGGCTGCCAACGAAGACCTTCCAGCTCCTACTTTTACACTTGACCAACTCGAGGCCAGCTTCTTAGCTGTAGGCCTCAACACCACGGATCTGGTTGCACTATCTGGTGCTCACACATTTGGGCGTGCTAGATGTTTAACTTTCACCGATCGATTGTATAACTTCAGCGGCACAGGCAGTCCTGATCCGACCTTGAACTCAACCTACTTAGAAACATTGAGTGAGATATGCCCACTGAATGGGAATGGCAGTGTACTGACCAATTTGGATCCTGTAACGCCTGACACTTTTGATGCAAATTATTTCTCGAATCTTCAAGTTGAGGAAGGCTTGCTCCAAAGCGATCAAGAGCTGTTTTCAACTAGCGGGGCTGATACCATCGACATTGTTAACAACTTCAGCGCTAATCAGAGCGCATTCTTTGAGAATTTCGGGGAATCCATGATTAAAATGGGTAATATAAGCCCGTTGACCGGAACCGAGGGGGAGATTAGATTGAACTGCCGTAGAGTTAATGGAGAATCATATGGATTAACTACTACTTTGGTAGCTGAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0020037 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0046906 GO:0048037 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097159 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901363 GO:1990748
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

352

Amino Acids

37.52

Weight (kDa)

4.2

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 300 1.3e-82 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 734
AciI CCGC 3 cut(s) 378, 663, 861
AclWI GGATC 6 cut(s) 170, 250, 591, 674, 758, 771
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 922
AcuI CTGAAG 2 cut(s) 643, 877
AfaI GTAC 3 cut(s) 265, 280, 750
AfeI AGCGCT 1 cut(s) 898
AfiI CCNNNNNNNGG 4 cut(s) 180, 547, 734, 968
AgsI TTSAA 5 cut(s) 158, 691, 812, 855, 994
AluBI AGCT 7 cut(s) 480, 488, 521, 555, 563, 847, 1046
AluI AGCT 7 cut(s) 480, 488, 521, 555, 563, 847, 1046
Alw21I GWGCWC 2 cut(s) 22, 607
AlwI GGATC 6 cut(s) 170, 250, 591, 674, 758, 771
Ama87I CYCGRG 1 cut(s) 545
Aor51HI AGCGCT 1 cut(s) 898
AoxI GGCC 3 cut(s) 321, 549, 568
ApeKI GCWGC 2 cut(s) 413, 500
ApoI RAATTY 1 cut(s) 922
Asp700I GAANNNNTTC 1 cut(s) 513
AspLEI GCGC 2 cut(s) 899, 911
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 95, 631
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 1 cut(s) 443
BaeI ACNNNNGTAYC 2 cut(s) 247, 280
BamHI GGATCC 1 cut(s) 763
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 2 cut(s) 22, 607
BbvI GCAGC 2 cut(s) 400, 487
BccI CCATC 2 cut(s) 233, 881
BceAI ACGGC 3 cut(s) 365, 513, 984
BcnI CCSGG 1 cut(s) 181
BfaI CTAG 2 cut(s) 624, 858
BfmI CTRYAG 2 cut(s) 167, 564
BfoI RGCGCY 1 cut(s) 900
BisI GCNGC 3 cut(s) 414, 501, 664
BlsI GCNGC 3 cut(s) 415, 502, 665
Bme1390I CCNGG 1 cut(s) 181
Bme18I GGWCC 1 cut(s) 443
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 2 cut(s) 765, 973
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 2 cut(s) 76, 778
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 646
BsaJI CCNNGG 3 cut(s) 179, 579, 975
BsaWI WCCGGW 1 cut(s) 968
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 547, 734, 968
Bse3DI GCAATG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 3 cut(s) 179, 579, 975
BseGI GGATG 1 cut(s) 83
BseLI CCNNNNNNNGG 4 cut(s) 180, 547, 734, 968
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 104, 1038
BseXI GCAGC 2 cut(s) 400, 487
BsgI GTGCAG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 646
BshFI GGCC 3 cut(s) 323, 551, 570
BshVI ATCGAT 1 cut(s) 646
BsiEI CGRYCG 1 cut(s) 646
BsiHKAI GWGCWC 2 cut(s) 22, 607
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 2 cut(s) 181, 969
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 4 cut(s) 180, 547, 734, 968
BsmFI GGGAC 1 cut(s) 360
BsmI GAATGC 3 cut(s) 133, 373, 911
BsnI GGCC 3 cut(s) 323, 551, 570
BsoBI CYCGRG 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 22, 607
Bsp143I GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
BspACI CCGC 3 cut(s) 378, 663, 861
BspANI GGCC 3 cut(s) 323, 551, 570
BspCNI CTCAG 2 cut(s) 103, 1039
BspDI ATCGAT 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 765, 973
BspMAI CTGCAG 1 cut(s) 171
BspPI GGATC 6 cut(s) 170, 250, 591, 674, 758, 771
BsrDI GCAATG 1 cut(s) 408
BssECI CCNNGG 3 cut(s) 179, 579, 975
BssMI GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
Bst4CI ACNGT 4 cut(s) 233, 263, 278, 457
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 4 cut(s) 193, 553, 621, 827
BstDEI CTNAG 4 cut(s) 90, 559, 703, 1047
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 900
BstHHI GCGC 2 cut(s) 899, 911
BstKTI GATC 7 cut(s) 178, 245, 586, 646, 682, 766, 841
BstMBI GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
BstMCI CGRYCG 1 cut(s) 646
BstMWI GCNNNNNNNGC 3 cut(s) 377, 413, 669
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 179
BstSFI CTRYAG 2 cut(s) 167, 564
BstV1I GCAGC 2 cut(s) 400, 487
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 2 cut(s) 583, 763
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 2 cut(s) 583, 763
Bsu15I ATCGAT 1 cut(s) 646
BsuRI GGCC 3 cut(s) 323, 551, 570
BsuTUI ATCGAT 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 579
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 83
BtsI GCAGTG 2 cut(s) 54, 751
BtsIMutI CAGTG 4 cut(s) 54, 462, 728, 751
Cac8I GCNNGC 4 cut(s) 193, 553, 621, 827
CfoI GCGC 2 cut(s) 899, 911
Cfr13I GGNCC 1 cut(s) 443
ClaI ATCGAT 1 cut(s) 646
Csp6I GTAC 3 cut(s) 264, 279, 749
CspCI CAANNNNNGTGG 2 cut(s) 121, 156
CviAII CATG 3 cut(s) 120, 450, 937
CviQI GTAC 3 cut(s) 264, 279, 749
DdeI CTNAG 4 cut(s) 90, 559, 703, 1047
DpnI GATC 7 cut(s) 177, 244, 585, 645, 681, 765, 840
DpnII GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 443
Eco47III AGCGCT 1 cut(s) 898
Eco57I CTGAAG 2 cut(s) 643, 877
Eco88I CYCGRG 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 443
FaeI CATG 3 cut(s) 123, 453, 940
FaqI GGGAC 1 cut(s) 360
FatI CATG 3 cut(s) 119, 449, 936
FauI CCCGC 2 cut(s) 385, 854
FauNDI CATATG 1 cut(s) 1021
Fnu4HI GCNGC 3 cut(s) 414, 501, 664
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 3 cut(s) 414, 501, 664
FspBI CTAG 2 cut(s) 624, 858
GlaI GCGC 2 cut(s) 898, 910
GluI GCNGC 3 cut(s) 414, 501, 664
GsuI CTGGAG 1 cut(s) 459
HaeII RGCGCY 1 cut(s) 900
HaeIII GGCC 3 cut(s) 323, 551, 570
HapII CCGG 2 cut(s) 181, 969
HhaI GCGC 2 cut(s) 899, 911
Hin1II CATG 3 cut(s) 123, 453, 940
Hin6I GCGC 2 cut(s) 897, 909
HinP1I GCGC 2 cut(s) 897, 909
HincII GTYRAC 4 cut(s) 96, 229, 886, 966
HindII GTYRAC 4 cut(s) 96, 229, 886, 966
HinfI GANTC 6 cut(s) 97, 150, 422, 805, 932, 1016
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 2 cut(s) 181, 969
HphI GGTGA 2 cut(s) 95, 631
Hpy166II GTNNAC 6 cut(s) 96, 229, 343, 749, 886, 966
Hpy188I TCNGA 2 cut(s) 684, 906
Hpy188III TCNNGA 6 cut(s) 215, 352, 438, 677, 802, 842
Hpy8I GTNNAC 6 cut(s) 96, 229, 343, 749, 886, 966
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 5 cut(s) 18, 456, 465, 524, 815
HpyCH4III ACNGT 4 cut(s) 233, 263, 278, 457
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 6 cut(s) 68, 74, 169, 359, 593, 791
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 413, 669
HpyF3I CTNAG 4 cut(s) 90, 559, 703, 1047
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 3 cut(s) 123, 453, 940
HspAI GCGC 2 cut(s) 897, 909
KspAI GTTAAC 1 cut(s) 886
Kzo9I GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
LmnI GCTCC 2 cut(s) 526, 834
Lsp1109I GCAGC 2 cut(s) 400, 487
LweI GCATC 2 cut(s) 76, 778
MaeI CTAG 2 cut(s) 624, 858
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 2 cut(s) 51, 769
MalI GATC 7 cut(s) 177, 244, 585, 645, 681, 765, 840
MboI GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
MboII GAAGA 5 cut(s) 170, 431, 480, 521, 800
MfeI CAATTG 1 cut(s) 23
MflI RGATCY 2 cut(s) 583, 763
MhlI GDGCHC 2 cut(s) 22, 607
MluCI AATT 5 cut(s) 23, 246, 757, 793, 922
MlyI GAGTC 2 cut(s) 91, 159
MmeI TCCRAC 1 cut(s) 707
MnlI CCTC 9 cut(s) 15, 154, 206, 320, 434, 541, 581, 811, 970
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 7 cut(s) 482, 632, 885, 942, 1008, 1028, 1054
MspA1I CMGCKG 1 cut(s) 663
MspI CCGG 2 cut(s) 181, 969
MspR9I CCNGG 1 cut(s) 181
MunI CAATTG 1 cut(s) 23
Mva1269I GAATGC 3 cut(s) 133, 373, 911
MwoI GCNNNNNNNGC 3 cut(s) 377, 413, 669
NciI CCSGG 1 cut(s) 181
NdeI CATATG 1 cut(s) 1021
NdeII GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
NlaIII CATG 3 cut(s) 123, 453, 940
NlaIV GGNNCC 2 cut(s) 765, 973
NmeAIII GCCGAG 2 cut(s) 181, 299
NspI RCATGY 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 545
PceI AGGCCT 1 cut(s) 570
PctI GAATGC 3 cut(s) 133, 373, 911
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 4 cut(s) 422, 805, 932, 1016
PflMI CCANNNNNTGG 1 cut(s) 734
PkrI GCNGC 3 cut(s) 415, 502, 665
Ple19I CGATCG 1 cut(s) 646
PleI GAGTC 2 cut(s) 91, 158
PpsI GAGTC 2 cut(s) 91, 158
PpuMI RGGWCCY 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 765, 973
PspPI GGNCC 1 cut(s) 443
PspPPI RGGWCCY 1 cut(s) 443
PspXI VCTCGAGB 1 cut(s) 545
PsrI GAACNNNNNNTAC 2 cut(s) 683, 715
PstI CTGCAG 1 cut(s) 171
PsuI RGATCY 2 cut(s) 583, 763
PvuI CGATCG 1 cut(s) 646
RsaI GTAC 3 cut(s) 265, 280, 750
RsaNI GTAC 3 cut(s) 264, 279, 749
SaqAI TTAA 7 cut(s) 482, 632, 885, 942, 1008, 1028, 1054
SatI GCNGC 3 cut(s) 414, 501, 664
Sau3AI GATC 7 cut(s) 175, 242, 583, 643, 679, 763, 838
Sau96I GGNCC 1 cut(s) 443
SchI GAGTC 2 cut(s) 91, 159
ScrFI CCNGG 1 cut(s) 181
SduI GDGCHC 2 cut(s) 22, 607
SfaNI GCATC 2 cut(s) 76, 778
SfcI CTRYAG 2 cut(s) 167, 564
Sfr274I CTCGAG 1 cut(s) 545
SinI GGWCC 1 cut(s) 443
SlaI CTCGAG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 5 cut(s) 23, 246, 757, 793, 922
SseBI AGGCCT 1 cut(s) 570
SsiI CCGC 3 cut(s) 378, 663, 861
SspMI CTAG 2 cut(s) 624, 858
StuI AGGCCT 1 cut(s) 570
StyD4I CCNGG 1 cut(s) 179
TaaI ACNGT 4 cut(s) 233, 263, 278, 457
TaiI ACGT 1 cut(s) 107
TaqI TCGA 4 cut(s) 546, 646, 803, 876
TaqII GACCGA 1 cut(s) 188
TasI AATT 5 cut(s) 23, 246, 757, 793, 922
TatI WGTACW 1 cut(s) 748
TauI GCSGC 1 cut(s) 666
TfiI GAWTC 4 cut(s) 422, 805, 932, 1016
Tru1I TTAA 7 cut(s) 482, 632, 885, 942, 1008, 1028, 1054
Tru9I TTAA 7 cut(s) 482, 632, 885, 942, 1008, 1028, 1054
TscAI CASTG 4 cut(s) 54, 462, 735, 751
TseI GCWGC 2 cut(s) 413, 500
TspDTI ATGAA 2 cut(s) 129, 438
TspGWI ACGGA 1 cut(s) 596
TspRI CASTG 4 cut(s) 54, 462, 735, 751
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 922
XceI RCATGY 1 cut(s) 123
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 2 cut(s) 624, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.