MD03G1132200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002690
Physical Location & Seq
Forward (+)
13122829 .. 13123997
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1132200.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGAATCGAAGCCAAGTTTGGAGAGCTCAAAGAACAAGGACAAGGAGCAGGGCAATAAGGACAAGTCTTTGTACAGGTTAGGTGCAATTGTTGCCGGTGCTGCTACAGGAATTGGTCTGCTGGCTTGGGGTTTATCTGCACTTTCTTCATCAGCTGGTTCAGAGTCAGAGGAAAAGAAGAAACCAATGAAAGCTCCTGGTTCACAGTCAGAGGAAAAGAAGAAACCAATGAAAGCTCCTGGAAAAGATGAAATCATAGAGAGGGAAGAGTTTGAGCGAGATCCTAAAGGCTATTTCAGAGCCTTGCGCGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.47

Weight (kDa)

9.27

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 309
AclWI GGATC 1 cut(s) 275
AfaI GTAC 1 cut(s) 74
AjnI CCWGG 2 cut(s) 196, 238
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 4 cut(s) 27, 155, 194, 236
AluI AGCT 4 cut(s) 27, 155, 194, 236
Alw21I GWGCWC 1 cut(s) 29
AlwI GGATC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 88
BciT130I CCWGG 2 cut(s) 198, 240
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
Bme1390I CCNGG 2 cut(s) 198, 240
BmrFI CCNGG 2 cut(s) 198, 240
Bse118I RCCGGY 1 cut(s) 95
BseBI CCWGG 2 cut(s) 198, 240
BseXI GCAGC 1 cut(s) 88
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 29
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 1 cut(s) 280
BspFNI CGCG 1 cut(s) 309
BspPI GGATC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 95
BssMI GATC 1 cut(s) 280
Bst2UI CCWGG 2 cut(s) 198, 240
Bst4CI ACNGT 1 cut(s) 207
Bst6I CTCTTC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 123
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 1 cut(s) 283
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 2 cut(s) 92, 101
BstNI CCWGG 2 cut(s) 198, 240
BstSCI CCNGG 2 cut(s) 196, 238
BstSFI CTRYAG 1 cut(s) 105
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 1 cut(s) 309
Cfr10I RCCGGY 1 cut(s) 95
Csp6I GTAC 1 cut(s) 73
CviJI RGCY 8 cut(s) 13, 27, 125, 155, 194, 236, 291, 302
CviKI_1 RGCY 8 cut(s) 13, 27, 125, 155, 194, 236, 291, 302
CviQI GTAC 1 cut(s) 73
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 196, 238
EcoT38I GRGCYC 1 cut(s) 29
FaiI YATR 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 102
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 102
GlaI GCGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 102
HapII CCGG 1 cut(s) 96
HhaI GCGC 1 cut(s) 309
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HinfI GANTC 2 cut(s) 5, 164
HpaII CCGG 1 cut(s) 96
Hpy166II GTNNAC 1 cut(s) 203
Hpy188I TCNGA 4 cut(s) 163, 169, 211, 299
Hpy8I GTNNAC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4V TGCA 2 cut(s) 86, 140
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 101
HspAI GCGC 1 cut(s) 307
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 3 cut(s) 46, 199, 241
Lsp1109I GCAGC 1 cut(s) 88
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 4 cut(s) 138, 190, 232, 278
MfeI CAATTG 1 cut(s) 87
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 2 cut(s) 87, 111
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 3 cut(s) 163, 205, 255
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 198, 240
MunI CAATTG 1 cut(s) 87
MvaI CCWGG 2 cut(s) 198, 240
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 2 cut(s) 92, 101
NdeII GATC 1 cut(s) 280
PfeI GAWTC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 238
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
Psp124BI GAGCTC 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 196, 238
PspGI CCWGG 2 cut(s) 196, 238
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SacI GAGCTC 1 cut(s) 29
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 1 cut(s) 280
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 2 cut(s) 198, 240
SduI GDGCHC 1 cut(s) 29
SetI ASST 6 cut(s) 29, 80, 85, 157, 196, 238
SfcI CTRYAG 1 cut(s) 105
Sse9I AATT 2 cut(s) 87, 111
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 2 cut(s) 196, 238
TaaI ACNGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 8
TasI AATT 2 cut(s) 87, 111
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 1 cut(s) 5
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 4 cut(s) 138, 203, 245, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.