RLG00000007858

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002690
Physical Location & Seq
Forward (+)
22076458 .. 22078420
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007858

Sequence Viewer

Length: 363 bp
ATGGGAGCTAATGAAAGCAGGGAAAGCTCTGGACAGGGTGAGAATGGTGGTGCGCCGTCCGGTAACCCTTGGTTCATTGCGGGACTAGCTGCTGTGGCTTTGGGCGCAGCGAAACTCGCTTTGGGTTCAACTTCTTCAGAAGAGCAGAGGAAAACCATGAAGGAGCCCGGGGGAAACGGGAGGATTTACAGGGACGATTTTGAGCATGCAGGCGTCTTCAGGTTTTGGTTTTCGTTCTCGTCGCGCTCTTGGACGCCGTCGTCGGCGATCAAGAGAACATGTTCTTGGTCAGACGGTGCACTCATGGCTTGGTATCTCTGTCGGTGCCCGACCCAGGCTGTGGCAGAGGACATTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

12.86

Weight (kDa)

5.81

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 324
AccB7I CCANNNNNTGG 1 cut(s) 340
AccII CGCG 1 cut(s) 244
AciI CCGC 1 cut(s) 80
AcuI CTGAAG 2 cut(s) 120, 202
AcyI GRCGYC 2 cut(s) 213, 254
AfiI CCNNNNNNNGG 2 cut(s) 334, 340
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 1 cut(s) 129
AjnI CCWGG 1 cut(s) 333
AjuI GAANNNNNNNTTGG 4 cut(s) 104, 136, 268, 300
AluBI AGCT 3 cut(s) 8, 27, 89
AluI AGCT 3 cut(s) 8, 27, 89
Alw21I GWGCWC 1 cut(s) 301
Alw44I GTGCAC 1 cut(s) 297
Ama87I CYCGRG 1 cut(s) 167
ApaLI GTGCAC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 89, 107
Asp700I GAANNNNTTC 1 cut(s) 280
AspLEI GCGC 3 cut(s) 55, 107, 246
AsuC2I CCSGG 2 cut(s) 168, 169
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 167
BaeGI GKGCMC 2 cut(s) 301, 329
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 2 cut(s) 76, 119
BceAI ACGGC 2 cut(s) 40, 241
BciT130I CCWGG 1 cut(s) 335
BcnI CCSGG 2 cut(s) 168, 169
BfaI CTAG 1 cut(s) 86
BisI GCNGC 2 cut(s) 90, 108
BlsI GCNGC 2 cut(s) 91, 109
Bme1390I CCNGG 3 cut(s) 168, 169, 335
BmeT110I CYCGRG 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 165, 326
BmrFI CCNGG 3 cut(s) 168, 169, 335
BpiI GAAGAC 1 cut(s) 208
BpuMI CCSGG 2 cut(s) 168, 169
BsaHI GRCGYC 2 cut(s) 213, 254
BsaJI CCNNGG 4 cut(s) 68, 167, 168, 333
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 340
Bse3DI GCAATG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 4 cut(s) 68, 167, 168, 333
BseLI CCNNNNNNNGG 2 cut(s) 334, 340
BseMI GCAATG 1 cut(s) 75
BseSI GKGCMC 2 cut(s) 301, 329
BseXI GCAGC 2 cut(s) 76, 119
Bsh1236I CGCG 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 301
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 2 cut(s) 60, 168
BslFI GGGAC 2 cut(s) 96, 206
BslI CCNNNNNNNGG 2 cut(s) 334, 340
BsmFI GGGAC 2 cut(s) 96, 206
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 3 cut(s) 168, 301, 329
Bsp143I GATC 1 cut(s) 267
BspACI CCGC 1 cut(s) 80
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 2 cut(s) 165, 326
BspQI GCTCTTC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 75
BssECI CCNNGG 4 cut(s) 68, 167, 168, 333
BssMI GATC 1 cut(s) 267
BssNI GRCGYC 2 cut(s) 213, 254
BssT1I CCWWGG 1 cut(s) 68
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 2 cut(s) 213, 254
BstC8I GCNNGC 2 cut(s) 207, 211
BstEII GGTNACC 1 cut(s) 62
BstFNI CGCG 1 cut(s) 244
BstHHI GCGC 3 cut(s) 55, 107, 246
BstKTI GATC 1 cut(s) 270
BstMBI GATC 1 cut(s) 267
BstMWI GCNNNNNNNGC 6 cut(s) 24, 86, 95, 104, 116, 305
BstNI CCWGG 1 cut(s) 335
BstNSI RCATGY 2 cut(s) 209, 282
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 3 cut(s) 166, 167, 333
BstSLI GKGCMC 2 cut(s) 301, 329
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 76, 119
BstV2I GAAGAC 1 cut(s) 208
Cac8I GCNNGC 2 cut(s) 207, 211
CfoI GCGC 3 cut(s) 55, 107, 246
Cfr9I CCCGGG 1 cut(s) 167
CseI GACGC 2 cut(s) 202, 262
CviAII CATG 4 cut(s) 157, 206, 279, 304
CviJI RGCY 7 cut(s) 8, 27, 89, 98, 166, 308, 338
CviKI_1 RGCY 7 cut(s) 8, 27, 89, 98, 166, 308, 338
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
DrdI GACNNNNNNGTC 1 cut(s) 259
DseDI GACNNNNNNGTC 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 120, 202
Eco88I CYCGRG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 68
FaeI CATG 4 cut(s) 160, 209, 282, 307
FaiI YATR 4 cut(s) 158, 207, 280, 305
FaqI GGGAC 2 cut(s) 96, 206
FatI CATG 4 cut(s) 156, 205, 278, 303
FauI CCCGC 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 90, 108
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 2 cut(s) 90, 108
FspBI CTAG 1 cut(s) 86
GlaI GCGC 3 cut(s) 54, 106, 245
GluI GCNGC 2 cut(s) 90, 108
HapII CCGG 2 cut(s) 60, 168
HgaI GACGC 2 cut(s) 202, 262
HhaI GCGC 3 cut(s) 55, 107, 246
Hin1I GRCGYC 2 cut(s) 213, 254
Hin1II CATG 4 cut(s) 160, 209, 282, 307
Hin6I GCGC 3 cut(s) 53, 105, 244
HinP1I GCGC 3 cut(s) 53, 105, 244
HpaII CCGG 2 cut(s) 60, 168
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 299
Hpy188I TCNGA 2 cut(s) 139, 292
Hpy188III TCNNGA 2 cut(s) 30, 271
Hpy8I GTNNAC 1 cut(s) 299
Hpy99I CGWCG 3 cut(s) 244, 262, 265
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 209, 299
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 86, 95, 104, 116, 305
Hsp92I GRCGYC 2 cut(s) 213, 254
Hsp92II CATG 4 cut(s) 160, 209, 282, 307
HspAI GCGC 3 cut(s) 53, 105, 244
Kzo9I GATC 1 cut(s) 267
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 2 cut(s) 5, 163
Lsp1109I GCAGC 2 cut(s) 76, 119
MaeI CTAG 1 cut(s) 86
MaeIII GTNAC 1 cut(s) 62
MalI GATC 1 cut(s) 269
MboI GATC 1 cut(s) 267
MboII GAAGA 3 cut(s) 126, 152, 208
MhlI GDGCHC 3 cut(s) 168, 301, 329
MnlI CCTC 3 cut(s) 141, 174, 340
MroXI GAANNNNTTC 1 cut(s) 280
MspI CCGG 2 cut(s) 60, 168
MspR9I CCNGG 3 cut(s) 168, 169, 335
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 6 cut(s) 24, 86, 95, 104, 116, 305
NciI CCSGG 2 cut(s) 168, 169
NdeII GATC 1 cut(s) 267
NlaIII CATG 4 cut(s) 160, 209, 282, 307
NlaIV GGNNCC 2 cut(s) 165, 326
NspI RCATGY 2 cut(s) 209, 282
PaeI GCATGC 1 cut(s) 209
PciI ACATGT 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 280
PflFI GACNNNGTC 1 cut(s) 256
PflMI CCANNNNNTGG 1 cut(s) 340
PkrI GCNGC 2 cut(s) 91, 109
PscI ACATGT 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 333
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 2 cut(s) 165, 326
PsyI GACNNNGTC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 2 cut(s) 90, 108
Sau3AI GATC 1 cut(s) 267
ScrFI CCNGG 3 cut(s) 168, 169, 335
SduI GDGCHC 3 cut(s) 168, 301, 329
SetI ASST 4 cut(s) 10, 29, 91, 224
SmaI CCCGGG 1 cut(s) 169
SphI GCATGC 1 cut(s) 209
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 3 cut(s) 166, 167, 333
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 1 cut(s) 296
TseI GCWGC 2 cut(s) 89, 107
TspDTI ATGAA 3 cut(s) 27, 64, 173
TspMI CCCGGG 1 cut(s) 167
Tth111I GACNNNGTC 1 cut(s) 256
Van91I CCANNNNNTGG 1 cut(s) 340
VneI GTGCAC 1 cut(s) 297
XceI RCATGY 2 cut(s) 209, 282
XmaI CCCGGG 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 280
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.