MD03G1225700.v1.1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003191
Physical Location & Seq
Forward (+)
31113615 .. 31114034
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1225700.v1.1.491

Sequence Viewer

Length: 420 bp
ATGATCACCAGATTACCGGAGGATATTTGCTGCCATATTCTCACATTCTTGCCAATTACACATGCCGTGGCCACTAGCGTGCTCTGCAAGAAGTGGAAAAACCTATGGACTTCTGCTTCCTTCACAAACCTAGAGCTTGATGACTGGTTACTGCTTCGCCTCGAAAGCTCCAACACCATGTACAACTACGCTTCCCTATTCGCCACCTTCGTTATCAACGTGCTCCGCCTCATCAATTTGCCATGCATACGAAAACTCGCATTCTTTTGCTCCGACCCTAACAATCTGAATTGCATAAATTCTTGGTTTCTTCGTGCATGCACATCCAATACAATTGAGGTCGAGATCAGGATTTCTTACACGAAAACCCGATTAAACTGCCTCAAATTTTGTATGTCAGCACTTCGCTTCTCGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

140

Amino Acids

16.16

Weight (kDa)

8.79

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 42 1.3e-09 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 226
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 298, 386
AfaI GTAC 2 cut(s) 182, 416
AleI CACNNNNGTG 1 cut(s) 77
AluBI AGCT 2 cut(s) 136, 168
AluI AGCT 2 cut(s) 136, 168
Alw21I GWGCWC 2 cut(s) 84, 225
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 30
ApoI RAATTY 2 cut(s) 298, 386
BalI TGGCCA 1 cut(s) 71
Bbv12I GWGCWC 2 cut(s) 84, 225
BbvI GCAGC 1 cut(s) 17
BceAI ACGGC 1 cut(s) 50
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BclI TGATCA 1 cut(s) 3
BfaI CTAG 2 cut(s) 75, 131
BisI GCNGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 149
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 84, 225
BsiSI CCGG 1 cut(s) 17
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 84, 225
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 2 cut(s) 3, 345
BspACI CCGC 1 cut(s) 226
BspANI GGCC 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 2 cut(s) 3, 345
BstAUI TGTACA 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 80, 319
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 323
BstKTI GATC 2 cut(s) 6, 348
BstMBI GATC 2 cut(s) 3, 345
BstMWI GCNNNNNNNGC 2 cut(s) 84, 165
BstNSI RCATGY 2 cut(s) 65, 321
BstV1I GCAGC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 80, 319
Csp6I GTAC 2 cut(s) 181, 415
CviAII CATG 4 cut(s) 62, 178, 243, 318
CviJI RGCY 3 cut(s) 71, 136, 168
CviKI_1 RGCY 3 cut(s) 71, 136, 168
CviQI GTAC 2 cut(s) 181, 415
DpnI GATC 2 cut(s) 5, 347
DpnII GATC 2 cut(s) 3, 345
EaeI YGGCCR 1 cut(s) 69
EciI GGCGGA 1 cut(s) 215
EcoT22I ATGCAT 1 cut(s) 248
FaeI CATG 4 cut(s) 65, 181, 246, 321
FaiI YATR 9 cut(s) 36, 63, 106, 179, 244, 248, 296, 319, 395
FatI CATG 4 cut(s) 61, 177, 242, 317
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 1 cut(s) 310
Fsp4HI GCNGC 1 cut(s) 31
FspBI CTAG 2 cut(s) 75, 131
GluI GCNGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 17
Hin1II CATG 4 cut(s) 65, 181, 246, 321
HpaII CCGG 1 cut(s) 17
Hpy188I TCNGA 2 cut(s) 274, 288
Hpy188III TCNNGA 2 cut(s) 343, 349
HpyAV CCTTC 2 cut(s) 130, 217
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 5 cut(s) 87, 246, 294, 317, 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 165
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 4 cut(s) 65, 181, 246, 321
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 345
LmnI GCTCC 3 cut(s) 173, 228, 275
LpnPI CCDG 4 cut(s) 22, 30, 130, 334
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 2 cut(s) 75, 131
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 147
MalI GATC 2 cut(s) 5, 347
MboI GATC 2 cut(s) 3, 345
MboII GAAGA 1 cut(s) 302
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 84, 225
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 6 cut(s) 54, 235, 289, 298, 333, 386
MluNI TGGCCA 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 195, 297
MnlI CCTC 5 cut(s) 13, 170, 239, 331, 392
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 248
MscI TGGCCA 1 cut(s) 71
MseI TTAA 1 cut(s) 374
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 17
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 84, 165
NdeII GATC 2 cut(s) 3, 345
NlaIII CATG 4 cut(s) 65, 181, 246, 321
NsiI ATGCAT 1 cut(s) 248
NspI RCATGY 2 cut(s) 65, 321
OliI CACNNNNGTG 1 cut(s) 77
PaeI GCATGC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 216
PctI GAATGC 1 cut(s) 260
PkrI GCNGC 1 cut(s) 32
RsaI GTAC 2 cut(s) 182, 416
RsaNI GTAC 2 cut(s) 181, 415
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 1 cut(s) 374
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 2 cut(s) 3, 345
SduI GDGCHC 2 cut(s) 84, 225
SetI ASST 7 cut(s) 105, 132, 138, 170, 209, 222, 342
SmiMI CAYNNNNRTG 1 cut(s) 77
SphI GCATGC 1 cut(s) 321
Sse9I AATT 6 cut(s) 54, 235, 289, 298, 333, 386
SsiI CCGC 1 cut(s) 226
SspMI CTAG 2 cut(s) 75, 131
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 162, 342
TasI AATT 6 cut(s) 54, 235, 289, 298, 333, 386
TatI WGTACW 1 cut(s) 180
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TseI GCWGC 1 cut(s) 30
XapI RAATTY 2 cut(s) 298, 386
XceI RCATGY 2 cut(s) 65, 321
XspI CTAG 2 cut(s) 75, 131
Zsp2I ATGCAT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.