MD03G1253900.v1.1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003191
Physical Location & Seq
Reverse (-)
34057531 .. 34058988
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1253900.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGGTTCGACATCGAAGTGTCAAGAGCGAGTTGATAGGATCAGTGAATTGCCAAATTCACTTCTTTGCCACATACTTTCATTCTTTCCGATATTAGATGCTGTGCAGACTACAATTCTGTCAAGACGATGGGAGAACTTATGGACTCCTCTTCCCAAACTTGAATTTGATGATGATTGCTGTTTCCCGAGTCTCAAGGTCCTAATTGTCCAGGTAGAGGATCTTGATGATTCAGTGGCAACTGCAAAGCTATTTTCTCATTGCCCTGTACTTGAAGATTTGAATATAGATGTAATTCCAAATGCTTATGCTTCAAATGTCAACATATCTGCACCTAGATTGAAGGCCTTAACAAAATCGTTGAACACTAACTATAATGAAAAGGACGGTGATTTTGTCCGTTTTTATATTGATGCTCCAAATCTGGAAAAGTTATCCATTGCGGCTGCCAATTCTCTCTTATTTTTGTTGGAAATTCAAATCAAATCAGGTTGTAAATGGCAGCTGCTAGCTGTTAGTTTCTTTACAGGTTGTATTCACACTTGCTGCAACTACAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

188

Amino Acids

21.06

Weight (kDa)

5.05

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 51 2.4e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 443
AclWI GGATC 2 cut(s) 47, 228
AcsI RAATTY 3 cut(s) 55, 164, 474
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 7 cut(s) 164, 275, 283, 315, 343, 364, 479
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 3 cut(s) 250, 505, 512
AluI AGCT 3 cut(s) 250, 505, 512
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 2 cut(s) 47, 228
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 4 cut(s) 446, 502, 505, 546
ApoI RAATTY 3 cut(s) 55, 164, 474
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 1 cut(s) 401
AsuNHI GCTAGC 1 cut(s) 508
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 1 cut(s) 199
BbvI GCAGC 4 cut(s) 433, 492, 514, 533
BccI CCATC 1 cut(s) 123
BciT130I CCWGG 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 2 cut(s) 336, 509
BisI GCNGC 5 cut(s) 444, 447, 503, 506, 547
BlsI GCNGC 5 cut(s) 445, 448, 504, 507, 548
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 88, 403
BmtI GCTAGC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse3DI GCAATG 2 cut(s) 259, 438
BseBI CCWGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 2 cut(s) 259, 438
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 433, 492, 514, 533
BsgI GTGCAG 2 cut(s) 125, 315
BshFI GGCC 1 cut(s) 347
BsiHKCI CYCGRG 1 cut(s) 187
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 187
Bsp143I GATC 2 cut(s) 39, 220
BspACI CCGC 1 cut(s) 443
BspANI GGCC 1 cut(s) 347
BspOI GCTAGC 1 cut(s) 512
BspPI GGATC 2 cut(s) 47, 228
BsrDI GCAATG 2 cut(s) 259, 438
BssMI GATC 2 cut(s) 39, 220
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 510
BstKTI GATC 2 cut(s) 42, 223
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 2 cut(s) 39, 220
BstNI CCWGG 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 210
BstV1I GCAGC 4 cut(s) 433, 492, 514, 533
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 1 cut(s) 347
BtsIMutI CAGTG 2 cut(s) 49, 240
Cac8I GCNNGC 1 cut(s) 510
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 269
CviJI RGCY 5 cut(s) 250, 347, 446, 505, 512
CviKI_1 RGCY 5 cut(s) 250, 347, 446, 505, 512
CviQI GTAC 1 cut(s) 269
DpnI GATC 2 cut(s) 41, 222
DpnII GATC 2 cut(s) 39, 220
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 210
FaiI YATR 7 cut(s) 74, 142, 287, 309, 326, 375, 408
Fnu4HI GCNGC 5 cut(s) 444, 447, 503, 506, 547
Fsp4HI GCNGC 5 cut(s) 444, 447, 503, 506, 547
FspBI CTAG 2 cut(s) 336, 509
GluI GCNGC 5 cut(s) 444, 447, 503, 506, 547
HaeIII GGCC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 3 cut(s) 145, 190, 230
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 5 cut(s) 23, 123, 187, 224, 425
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4V TGCA 4 cut(s) 106, 245, 332, 549
Kzo9I GATC 2 cut(s) 39, 220
LmnI GCTCC 1 cut(s) 421
LpnPI CCDG 6 cut(s) 197, 224, 279, 410, 474, 513
Lsp1109I GCAGC 4 cut(s) 433, 492, 514, 533
LweI GCATC 2 cut(s) 88, 403
MaeI CTAG 2 cut(s) 336, 509
MalI GATC 2 cut(s) 41, 222
MboI GATC 2 cut(s) 39, 220
MboII GAAGA 2 cut(s) 143, 287
MflI RGATCY 1 cut(s) 220
MluCI AATT 8 cut(s) 47, 55, 114, 164, 204, 294, 451, 474
MlyI GAGTC 2 cut(s) 139, 199
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 2 cut(s) 159, 211
MseI TTAA 1 cut(s) 350
MslI CAYNNNNRTG 1 cut(s) 16
MspA1I CMGCKG 1 cut(s) 505
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
NdeII GATC 2 cut(s) 39, 220
NheI GCTAGC 1 cut(s) 508
PceI AGGCCT 1 cut(s) 347
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 5 cut(s) 445, 448, 504, 507, 548
PleI GAGTC 2 cut(s) 139, 198
PpsI GAGTC 2 cut(s) 139, 198
PpuMI RGGWCCY 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspPI GGNCC 1 cut(s) 199
PspPPI RGGWCCY 1 cut(s) 199
PsuI RGATCY 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 16
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 5 cut(s) 444, 447, 503, 506, 547
Sau3AI GATC 2 cut(s) 39, 220
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 2 cut(s) 139, 199
ScrFI CCNGG 1 cut(s) 212
SetI ASST 8 cut(s) 201, 216, 252, 337, 493, 507, 514, 532
SfaNI GCATC 2 cut(s) 88, 403
SinI GGWCC 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 8 cut(s) 47, 55, 114, 164, 204, 294, 451, 474
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 1 cut(s) 443
SspMI CTAG 2 cut(s) 336, 509
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 8, 14
TasI AATT 8 cut(s) 47, 55, 114, 164, 204, 294, 451, 474
TatI WGTACW 1 cut(s) 268
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 2 cut(s) 49, 240
TseI GCWGC 4 cut(s) 446, 502, 505, 546
TspDTI ATGAA 2 cut(s) 69, 393
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 2 cut(s) 49, 240
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 3 cut(s) 55, 164, 474
XspI CTAG 2 cut(s) 336, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.