RchiOBHm_Chr5g0033891

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003191
Physical Location & Seq
Forward (+)
27788649 .. 27790508
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31293

Sequence Viewer

Length: 1440 bp
ATGATCACCGAATTACCAGAAGACGTTCGCCGCCACATTCTCTCGTTCTTGTCAACTAAACACGCCGTGTCCACTACCATCCTTTCAAGAAGTTGGAAAAACCTATGGACCTTGGCTTCAACCAAAAACCTAGAGCTTGACGACAGCCTACTCCTCCATCCCGAAAGTAATTCCGAAACCATTCACCACCTTCCTTCTTTATTCTCTAATTTCGTGAACCATGTTCTCGGCCTCATCAGTGTGCCATGTATTCAAACACTCAAACTCTGTTGCTCCTACCCTTATGACCCAGATCACATAAATTGTTGGTTACATACGGCATGCAAATACAATGTCAGTGAGGTTAATATCAGAGTATACAGTCAAAACCCTATCAAATTGCCTTCGAATTTGTATGGTAGTACTTGGCTGGTGGTACTGAAATTAAACGCGAATGTAAGTCTCGACTTTCGGGACTCCATCTCTTTTCCAAGACTCAAGGTGCTTTGGATAAAGGTTTATGACGCAAACGATGTTAACTTGACGCAGAAGCTTTTCAGAGATCACATCTGCCCTATTCTTGAAGAGTTGTTCATAGAGGGTGAAATATTAGATGAGAATAGAAGAGTGGTTTTGTTGGTGATATTGAGTTCACTTAAGAGGTTGAAAATTGAATACCGTTTGGCACATAGTTTTCAGGGTGGTATTGAGTACAATTTTGTGTTGCATTGCCCTAACCTTGAGTGCCTTGATCTCCGTGATGACTTCTTGGCCAAGTATAGTGCTAGGGATTTGAAAGTTCTTGACTATGCAAAGATTAGTATTGGATACCCCTGTGCAGATTATGCAGCATTTGATCTTTATGGCGATGTAAATCCCTCTAATCGTGCGTTTGAGGTCCTCGAAGAACTCTCGAATGTCAAGTCTCTATCATTATCTGGTGGTACTACAAAGGCTCTCAGTTGTTCATATGAGACTGCACAACATACCGGTTTAGATGATGACGATGAAAATGCATTACTCATCCGCTTGAGAAATTTTGAGCTGTATTTTAGATTTTTGACCTTTTTGGAGCTGGGTTTCCGTACCTGTGAGAGCTGGAGTTTATTGCCCTTATTACTGCATAGCTCGCCTAATGTGGAGTACCTTATCTTAAAGAAGGAATTGGATTTAACTAGTTGTCGTTACAAAACAGTATTCTTCTGGAGACCGGAAGAACCTGTTCCGGAATGCATGTCTCTTCATCTCAGAGAAATCGAGATCAGGGGATTTCAGGGGGAATCGCAGGAGTTGGCAATTGTAGAGTTTTTTTTGGGGCATGCAGAAGTGTTGCAAAAGATGACTATTCATGTCCAAGAAGCATCACCAAATATGATGTTTTCCGAGCATGTATTACAAATCCCCACAGTTGCAGAAGCATGTGAAGTGGAGATAATATCAACAAAAAGTTGCATACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

479

Amino Acids

55.02

Weight (kDa)

5.42

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 37 4.4e-07 F-box domain
FBD PF08387 399 - 442 2.9e-13 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 357
AccII CGCG 1 cut(s) 431
AccIII TCCGGA 1 cut(s) 1204
AciI CCGC 2 cut(s) 31, 1006
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 2 cut(s) 388, 1015
AdeI CACNNNGTG 1 cut(s) 67
AfaI GTAC 6 cut(s) 403, 417, 692, 925, 1066, 1124
AflII CTTAAG 1 cut(s) 635
AgeI ACCGGT 1 cut(s) 968
AgsI TTSAA 7 cut(s) 87, 120, 254, 563, 646, 653, 775
AhlI ACTAGT 1 cut(s) 1154
AluBI AGCT 6 cut(s) 136, 532, 1024, 1054, 1077, 1107
AluI AGCT 6 cut(s) 136, 532, 1024, 1054, 1077, 1107
Alw26I GTCTC 5 cut(s) 446, 909, 947, 1180, 1221
Aor13HI TCCGGA 1 cut(s) 1204
AoxI GGCC 2 cut(s) 229, 750
ApeKI GCWGC 1 cut(s) 827
ApoI RAATTY 2 cut(s) 388, 1015
ArsI GACNNNNNNTTYG 4 cut(s) 318, 350, 887, 919
AsiGI ACCGGT 1 cut(s) 968
Asp700I GAANNNNTTC 4 cut(s) 24, 180, 533, 1200
AspS9I GGNCC 2 cut(s) 108, 877
AsuHPI GGTGA 4 cut(s) 176, 593, 631, 1335
AsuII TTCGAA 1 cut(s) 386
AvaII GGWCC 2 cut(s) 108, 877
BaeI ACNNNNGTAYC 2 cut(s) 799, 832
BalI TGGCCA 1 cut(s) 752
BbsI GAAGAC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 839
BccI CCATC 3 cut(s) 86, 165, 467
BceAI ACGGC 2 cut(s) 50, 333
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciVI GTATCC 1 cut(s) 800
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 5 cut(s) 446, 909, 947, 1180, 1221
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 3 cut(s) 131, 765, 1155
BfrI CTTAAG 1 cut(s) 635
BfuI GTATCC 1 cut(s) 800
BisI GCNGC 2 cut(s) 31, 828
BlsI GCNGC 2 cut(s) 32, 829
BmcAI AGTACT 1 cut(s) 403
Bme18I GGWCC 2 cut(s) 108, 877
BmgT120I GGNCC 2 cut(s) 108, 877
BmsI GCATC 1 cut(s) 1349
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 2 cut(s) 1099, 1204
Bpu14I TTCGAA 1 cut(s) 386
BpuEI CTTGAG 3 cut(s) 461, 740, 1030
BsaBI GATNNNNATC 1 cut(s) 852
BsaI GGTCTC 1 cut(s) 1180
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 3 cut(s) 968, 1189, 1204
Bse118I RCCGGY 1 cut(s) 968
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 852
BseAI TCCGGA 1 cut(s) 1204
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 3 cut(s) 78, 157, 1002
BseJI GATNNNNATC 1 cut(s) 852
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 2 cut(s) 952, 1240
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 839
BseYI CCCAGC 1 cut(s) 1054
BsgI GTGCAG 2 cut(s) 837, 942
Bsh1236I CGCG 1 cut(s) 431
BshFI GGCC 2 cut(s) 231, 752
BshTI ACCGGT 1 cut(s) 968
BsiSI CCGG 3 cut(s) 969, 1190, 1205
BslFI GGGAC 1 cut(s) 467
BsmAI GTCTC 5 cut(s) 446, 909, 947, 1180, 1221
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 1214
BsnI GGCC 2 cut(s) 231, 752
Bso31I GGTCTC 1 cut(s) 1180
Bsp119I TTCGAA 1 cut(s) 386
Bsp13I TCCGGA 1 cut(s) 1204
Bsp143I GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
BspACI CCGC 2 cut(s) 31, 1006
BspANI GGCC 2 cut(s) 231, 752
BspCNI CTCAG 2 cut(s) 951, 1239
BspEI TCCGGA 1 cut(s) 1204
BspFNI CGCG 1 cut(s) 431
BspT104I TTCGAA 1 cut(s) 386
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 1180
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 1 cut(s) 968
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
BssNAI GTATAC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 111
Bst1107I GTATAC 1 cut(s) 358
Bst4CI ACNGT 4 cut(s) 362, 659, 1174, 1387
Bst6I CTCTTC 3 cut(s) 558, 598, 1224
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 1 cut(s) 824
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 322, 1109, 1299
BstDEI CTNAG 2 cut(s) 938, 1226
BstF5I GGATG 3 cut(s) 78, 157, 1002
BstFNI CGCG 1 cut(s) 431
BstKTI GATC 6 cut(s) 6, 295, 544, 733, 838, 1242
BstMAI GTCTC 5 cut(s) 446, 909, 947, 1180, 1221
BstMBI GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
BstMWI GCNNNNNNNGC 2 cut(s) 824, 1108
BstNSI RCATGY 5 cut(s) 324, 1216, 1301, 1370, 1401
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 839
BstV2I GAAGAC 1 cut(s) 27
BstZ17I GTATAC 1 cut(s) 358
BsuI GTATCC 1 cut(s) 800
BsuRI GGCC 2 cut(s) 231, 752
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 3 cut(s) 78, 157, 1002
BtsIMutI CAGTG 2 cut(s) 244, 343
Cac8I GCNNGC 3 cut(s) 322, 1109, 1299
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 2 cut(s) 108, 877
CseI GACGC 2 cut(s) 512, 532
Csp6I GTAC 6 cut(s) 402, 416, 691, 924, 1065, 1123
CspAI ACCGGT 1 cut(s) 968
CviAII CATG 8 cut(s) 221, 246, 321, 1213, 1298, 1328, 1367, 1398
CviQI GTAC 6 cut(s) 402, 416, 691, 924, 1065, 1123
DdeI CTNAG 2 cut(s) 938, 1226
DpnI GATC 6 cut(s) 5, 294, 543, 732, 837, 1241
DpnII GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
DraIII CACNNNGTG 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 750
Eam1104I CTCTTC 3 cut(s) 558, 598, 1224
EarI CTCTTC 3 cut(s) 558, 598, 1224
Eco130I CCWWGG 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 1180
Eco47I GGWCC 2 cut(s) 108, 877
EcoO109I RGGNCCY 1 cut(s) 877
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 2 cut(s) 997, 1214
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 8 cut(s) 224, 249, 324, 1216, 1301, 1331, 1370, 1401
FaqI GGGAC 1 cut(s) 467
FatI CATG 8 cut(s) 220, 245, 320, 1212, 1297, 1327, 1366, 1397
FauNDI CATATG 1 cut(s) 949
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 31, 828
FokI GGATG 3 cut(s) 65, 144, 989
Fsp4HI GCNGC 2 cut(s) 31, 828
FspBI CTAG 3 cut(s) 131, 765, 1155
GluI GCNGC 2 cut(s) 31, 828
GsaI CCCAGC 1 cut(s) 1058
GsuI CTGGAG 2 cut(s) 1099, 1204
HaeIII GGCC 2 cut(s) 231, 752
HapII CCGG 3 cut(s) 969, 1190, 1205
HgaI GACGC 2 cut(s) 512, 532
Hin1II CATG 8 cut(s) 224, 249, 324, 1216, 1301, 1331, 1370, 1401
HincII GTYRAC 2 cut(s) 54, 517
HindII GTYRAC 2 cut(s) 54, 517
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 3 cut(s) 455, 474, 1259
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 3 cut(s) 969, 1190, 1205
HphI GGTGA 4 cut(s) 176, 593, 631, 1335
Hpy166II GTNNAC 6 cut(s) 54, 72, 217, 358, 517, 632
Hpy188I TCNGA 5 cut(s) 175, 353, 539, 1229, 1363
Hpy8I GTNNAC 6 cut(s) 54, 72, 217, 358, 517, 632
HpyAV CCTTC 4 cut(s) 200, 204, 393, 1132
HpyCH4III ACNGT 4 cut(s) 362, 659, 1174, 1387
HpyCH4IV ACGT 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 2 cut(s) 824, 1108
HpyF3I CTNAG 2 cut(s) 938, 1226
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 8 cut(s) 224, 249, 324, 1216, 1301, 1331, 1370, 1401
Kpn2I TCCGGA 1 cut(s) 1204
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 517
Kzo9I GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
LmnI GCTCC 2 cut(s) 278, 1051
Lsp1109I GCAGC 1 cut(s) 839
LweI GCATC 1 cut(s) 1349
MaeI CTAG 3 cut(s) 131, 765, 1155
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 309, 1163
MalI GATC 6 cut(s) 5, 294, 543, 732, 837, 1241
MboI GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
MboII GAAGA 7 cut(s) 32, 575, 615, 896, 1171, 1205, 1211
MfeI CAATTG 1 cut(s) 1275
MlsI TGGCCA 1 cut(s) 752
MluNI TGGCCA 1 cut(s) 752
MlyI GAGTC 2 cut(s) 449, 468
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 8 cut(s) 164, 242, 334, 571, 633, 868, 868, 890
Mox20I TGGCCA 1 cut(s) 752
Mph1103I ATGCAT 2 cut(s) 997, 1214
MroI TCCGGA 1 cut(s) 1204
MroXI GAANNNNTTC 4 cut(s) 24, 180, 533, 1200
MscI TGGCCA 1 cut(s) 752
MseI TTAA 6 cut(s) 345, 425, 516, 636, 1133, 1151
MslI CAYNNNNRTG 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 752
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 3 cut(s) 969, 1190, 1205
MunI CAATTG 1 cut(s) 1275
Mva1269I GAATGC 1 cut(s) 1214
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 2 cut(s) 824, 1108
NdeI CATATG 1 cut(s) 949
NdeII GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
NlaIII CATG 8 cut(s) 224, 249, 324, 1216, 1301, 1331, 1370, 1401
NmeAIII GCCGAG 1 cut(s) 207
NsiI ATGCAT 2 cut(s) 997, 1214
NspI RCATGY 5 cut(s) 324, 1216, 1301, 1370, 1401
NspV TTCGAA 1 cut(s) 386
PaeI GCATGC 2 cut(s) 324, 1301
PctI GAATGC 1 cut(s) 1214
PdmI GAANNNNTTC 4 cut(s) 24, 180, 533, 1200
PfeI GAWTC 1 cut(s) 1259
PinAI ACCGGT 1 cut(s) 968
PkrI GCNGC 2 cut(s) 32, 829
PleI GAGTC 2 cut(s) 449, 468
PpsI GAGTC 2 cut(s) 449, 468
PpuMI RGGWCCY 1 cut(s) 877
Psp5II RGGWCCY 1 cut(s) 877
PspFI CCCAGC 1 cut(s) 1054
PspPI GGNCC 2 cut(s) 108, 877
PspPPI RGGWCCY 1 cut(s) 877
RsaI GTAC 6 cut(s) 403, 417, 692, 925, 1066, 1124
RsaNI GTAC 6 cut(s) 402, 416, 691, 924, 1065, 1123
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 6 cut(s) 345, 425, 516, 636, 1133, 1151
SatI GCNGC 2 cut(s) 31, 828
Sau3AI GATC 6 cut(s) 3, 292, 541, 730, 835, 1239
Sau96I GGNCC 2 cut(s) 108, 877
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 2 cut(s) 449, 468
SfaNI GCATC 1 cut(s) 1349
SfuI TTCGAA 1 cut(s) 386
SinI GGWCC 2 cut(s) 108, 877
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 4 cut(s) 476, 635, 719, 1009
SmoI CTYRAG 4 cut(s) 476, 635, 719, 1009
SpeI ACTAGT 1 cut(s) 1154
SphI GCATGC 2 cut(s) 324, 1301
SsiI CCGC 2 cut(s) 31, 1006
SspI AATATT 1 cut(s) 588
SspMI CTAG 3 cut(s) 131, 765, 1155
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 4 cut(s) 362, 659, 1174, 1387
TaiI ACGT 1 cut(s) 27
TaqI TCGA 5 cut(s) 386, 444, 882, 893, 1236
TatI WGTACW 2 cut(s) 401, 690
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 1 cut(s) 1259
Tru1I TTAA 6 cut(s) 345, 425, 516, 636, 1133, 1151
Tru9I TTAA 6 cut(s) 345, 425, 516, 636, 1133, 1151
TscAI CASTG 2 cut(s) 244, 343
TseI GCWGC 1 cut(s) 827
TspDTI ATGAA 5 cut(s) 562, 936, 1002, 1211, 1316
TspGWI ACGGA 2 cut(s) 725, 1052
TspRI CASTG 2 cut(s) 244, 343
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 108, 877
XapI RAATTY 2 cut(s) 388, 1015
XceI RCATGY 5 cut(s) 324, 1216, 1301, 1370, 1401
XmiI GTMKAC 1 cut(s) 357
XmnI GAANNNNTTC 4 cut(s) 24, 180, 533, 1200
XspI CTAG 3 cut(s) 131, 765, 1155
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 2 cut(s) 997, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.