Rorug05G0333700

peroxisome matrix targeting signal-2 binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003191
Physical Location & Seq
Forward (+)
40356284 .. 40357222
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0333700.1

Sequence Viewer

Length: 939 bp
ATGGCGACATTAAGATCCAGGGTTCCAAATCGCTCCAATAAACAAGATTTCAAGAATCGATCGTCAACAACCAACAAATCAGGTCTCCCAGTCGGGGTCAGGTTCCGTCCTACAGACCACGAACTCCTCGATTACTATCTCCACACCAAGATCGCCATGGGTGACCACTTTCACTCCGATTTCGTTGCCGATTTTCCTGATTTTTACTGCGAGAATGAGCCCTGGGTTGTTTGGCAGATCTACTGCGGCGGTGAGTCCAAGAAAGTGGAGGATGAAAAGACTCTCTATTTCTTCACCCATCGGAGAAGGCTGAGTCCCACCGCCAAGCGCTTTGATCGGAAAGTGGGTTCCGGCACTTGGAGCGGCCAGTGTTCGAAAGAAGTTGTTGACCATGAGGATGATGGTGTTGTTATTGGGCTCAAGAGGGAGTTCCGATATGAGGGCGGTTGTGATTCTCGTCACAACGAGGCTTGGTTGATGCAAGAGTACCAGATCATTGATGATGATTCTGATATTGTACTGTGCACTTTGAGAAAGAACCCTAGAAAGGCACCACCTGCTCAGAGGAGTAATGTTGTTGAAAAGACCAGAGTTGATGACAAAAAGAGAAAGAGCATTGTGGATCAACCCGGAAAGCCTAAATGGAAAAAGCAGAAAAAGGAAGAGAGTTGTGATCAGGAACAAGAAGGCAGCAGTACTGTTGATCAGATGAAAGTGGAAGAGACCTGTTACGAGTTGGATCAAGATCATGGACAGCTGCAAGTAGTGTTTCCCTATCCGTTTCAAGATGATCAAGATTGTTATTATCCTGAGCTTTATCCTGAGCTGTGTGTCGATGATAAGCCTGATTTATTTACTATTGATGAATTACTTGGACAAACCCCAACTTGGTATGAGGGCAACAACTTCAATTTGGTCACAGGAGGACCACTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000268 GO:0001501 GO:0001503 GO:0001764 GO:0001958 GO:0003674 GO:0005048 GO:0005053 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006662 GO:0006810 GO:0006886 GO:0006928 GO:0006996 GO:0007031 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0008152 GO:0008610 GO:0008611 GO:0009056 GO:0009058 GO:0009062 GO:0009653 GO:0009887 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016042 GO:0016043 GO:0016054 GO:0016477 GO:0016558 GO:0016560 GO:0017038 GO:0018904 GO:0019395 GO:0019752 GO:0019899 GO:0022008 GO:0030154 GO:0030258 GO:0031090 GO:0031461 GO:0031903 GO:0031907 GO:0031974 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034440 GO:0034613 GO:0036075 GO:0040011 GO:0042277 GO:0042579 GO:0042802 GO:0042803 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046485 GO:0046504 GO:0046907 GO:0046983 GO:0048513 GO:0048699 GO:0048705 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051674 GO:0055085 GO:0055114 GO:0060348 GO:0060349 GO:0060350 GO:0065002 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0080008 GO:0097384 GO:0098588 GO:0098805 GO:1901503 GO:1901575 GO:1901576 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
WD40

Protein Analysis

312

Amino Acids

36.32

Weight (kDa)

5.32

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 30 - 165 7.1e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 550
AccBSI CCGCTC 1 cut(s) 363
AciI CCGC 5 cut(s) 246, 249, 321, 363, 444
AclWI GGATC 3 cut(s) 9, 630, 747
AcoI YGGCCR 1 cut(s) 364
AfaI GTAC 3 cut(s) 488, 519, 697
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 5 cut(s) 94, 357, 439, 547, 888
AgsI TTSAA 4 cut(s) 52, 581, 785, 910
AjnI CCWGG 2 cut(s) 17, 221
AloI GAACNNNNNNTCC 2 cut(s) 331, 363
AluBI AGCT 3 cut(s) 757, 814, 826
AluI AGCT 3 cut(s) 757, 814, 826
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 2 cut(s) 89, 716
Alw44I GTGCAC 1 cut(s) 523
AlwI GGATC 3 cut(s) 9, 630, 747
Aor51HI AGCGCT 1 cut(s) 329
AoxI GGCC 1 cut(s) 364
ApaLI GTGCAC 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 690, 757
ArsI GACNNNNNNTTYG 1 cut(s) 918
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 926
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 3 cut(s) 173, 263, 286
AsuII TTCGAA 1 cut(s) 374
AvaII GGWCC 1 cut(s) 926
BaeGI GKGCMC 1 cut(s) 527
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 2 cut(s) 222, 420
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 2 cut(s) 702, 744
BccI CCATC 2 cut(s) 306, 395
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 2 cut(s) 19, 223
BclI TGATCA 3 cut(s) 673, 703, 790
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 89, 716
BfaI CTAG 1 cut(s) 543
BfmI CTRYAG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 565
BglII AGATCT 1 cut(s) 237
BisI GCNGC 4 cut(s) 247, 364, 691, 758
BlsI GCNGC 4 cut(s) 248, 365, 692, 759
BmcAI AGTACT 1 cut(s) 697
Bme1390I CCNGG 3 cut(s) 19, 223, 630
Bme18I GGWCC 1 cut(s) 926
BmgT120I GGNCC 1 cut(s) 926
BmiI GGNNCC 4 cut(s) 24, 104, 349, 552
BmrFI CCNGG 3 cut(s) 19, 223, 630
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 83
Bpu10I CCTNAGC 2 cut(s) 810, 822
Bpu14I TTCGAA 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 630
Bsa29I ATCGAT 1 cut(s) 58
BsaBI GATNNNNATC 2 cut(s) 135, 744
BsaI GGTCTC 2 cut(s) 89, 716
BsaJI CCNNGG 4 cut(s) 18, 156, 221, 222
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 357, 439, 547, 888
Bse1I ACTGG 2 cut(s) 89, 367
Bse8I GATNNNNATC 2 cut(s) 135, 744
BseBI CCWGG 2 cut(s) 19, 223
BseCI ATCGAT 1 cut(s) 58
BseDI CCNNGG 4 cut(s) 18, 156, 221, 222
BseGI GGATG 2 cut(s) 277, 403
BseJI GATNNNNATC 2 cut(s) 135, 744
BseLI CCNNNNNNNGG 5 cut(s) 94, 357, 439, 547, 888
BseMII CTCAG 4 cut(s) 302, 575, 801, 813
BseNI ACTGG 2 cut(s) 89, 367
BseRI GAGGAG 2 cut(s) 116, 580
BseSI GKGCMC 1 cut(s) 527
BseXI GCAGC 2 cut(s) 702, 744
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 366
BshNI GGYRCC 1 cut(s) 550
BshVI ATCGAT 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 527
BsiSI CCGG 2 cut(s) 351, 630
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 5 cut(s) 94, 357, 439, 547, 888
BsmAI GTCTC 2 cut(s) 89, 716
BsmFI GGGAC 1 cut(s) 300
BsnI GGCC 1 cut(s) 366
Bso31I GGTCTC 2 cut(s) 89, 716
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 222, 420, 527
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 5 cut(s) 246, 249, 321, 363, 444
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 4 cut(s) 303, 574, 802, 814
BspDI ATCGAT 1 cut(s) 58
BspLI GGNNCC 4 cut(s) 24, 104, 349, 552
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 3 cut(s) 9, 630, 747
BspT104I TTCGAA 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 550
BspTNI GGTCTC 2 cut(s) 89, 716
BsrBI CCGCTC 1 cut(s) 363
BsrI ACTGG 2 cut(s) 89, 367
BssECI CCNNGG 4 cut(s) 18, 156, 221, 222
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 19, 223
Bst4CI ACNGT 2 cut(s) 522, 700
Bst6I CTCTTC 2 cut(s) 657, 714
BstAPI GCANNNNNTGC 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 374
BstDEI CTNAG 4 cut(s) 311, 561, 810, 822
BstDSI CCRYGG 1 cut(s) 156
BstEII GGTNACC 1 cut(s) 161
BstF5I GGATG 2 cut(s) 277, 403
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstMAI GTCTC 2 cut(s) 89, 716
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 360, 557
BstNI CCWGG 2 cut(s) 19, 223
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 17, 221, 628
BstSFI CTRYAG 1 cut(s) 111
BstSLI GKGCMC 1 cut(s) 527
BstV1I GCAGC 2 cut(s) 702, 744
BstX2I RGATCY 2 cut(s) 14, 237
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 2 cut(s) 14, 237
Bsu15I ATCGAT 1 cut(s) 58
BsuRI GGCC 1 cut(s) 366
BsuTUI ATCGAT 1 cut(s) 58
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 2 cut(s) 277, 403
BtsIMutI CAGTG 1 cut(s) 374
BveI ACCTGC 1 cut(s) 565
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 926
ClaI ATCGAT 1 cut(s) 58
Csp6I GTAC 3 cut(s) 487, 518, 696
CviAII CATG 3 cut(s) 157, 392, 749
CviQI GTAC 3 cut(s) 487, 518, 696
DdeI CTNAG 4 cut(s) 311, 561, 810, 822
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 657, 714
EarI CTCTTC 2 cut(s) 657, 714
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 2 cut(s) 222, 420
Eco31I GGTCTC 2 cut(s) 89, 716
Eco47I GGWCC 1 cut(s) 926
Eco47III AGCGCT 1 cut(s) 329
Eco91I GGTNACC 1 cut(s) 161
EcoO65I GGTNACC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 17, 221
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 222, 420
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 3 cut(s) 160, 395, 752
FaiI YATR 5 cut(s) 158, 393, 438, 750, 894
FaqI GGGAC 1 cut(s) 300
FatI CATG 3 cut(s) 156, 391, 748
FbaI TGATCA 3 cut(s) 673, 703, 790
Fnu4HI GCNGC 4 cut(s) 247, 364, 691, 758
FokI GGATG 2 cut(s) 284, 410
FriOI GRGCYC 2 cut(s) 222, 420
Fsp4HI GCNGC 4 cut(s) 247, 364, 691, 758
FspBI CTAG 1 cut(s) 543
GlaI GCGC 1 cut(s) 329
GluI GCNGC 4 cut(s) 247, 364, 691, 758
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 1 cut(s) 366
HapII CCGG 2 cut(s) 351, 630
HhaI GCGC 1 cut(s) 330
Hin1II CATG 3 cut(s) 160, 395, 752
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 2 cut(s) 66, 388
HindII GTYRAC 2 cut(s) 66, 388
HinfI GANTC 6 cut(s) 55, 254, 280, 313, 452, 506
HpaII CCGG 2 cut(s) 351, 630
HphI GGTGA 3 cut(s) 173, 263, 286
Hpy166II GTNNAC 3 cut(s) 66, 388, 525
Hpy188I TCNGA 7 cut(s) 178, 303, 339, 434, 511, 564, 708
Hpy188III TCNNGA 9 cut(s) 52, 197, 421, 677, 743, 785, 794, 809, 821
Hpy8I GTNNAC 3 cut(s) 66, 388, 525
HpyAV CCTTC 2 cut(s) 300, 680
HpyCH4III ACNGT 2 cut(s) 522, 700
HpyCH4V TGCA 3 cut(s) 481, 525, 760
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 557
HpyF3I CTNAG 4 cut(s) 311, 561, 810, 822
Hsp92II CATG 3 cut(s) 160, 395, 752
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 3 cut(s) 673, 703, 790
LmnI GCTCC 2 cut(s) 38, 360
Lsp1109I GCAGC 2 cut(s) 702, 744
LweI GCATC 1 cut(s) 468
MaeI CTAG 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 161, 458, 728, 916
MbiI CCGCTC 1 cut(s) 363
MboII GAAGA 3 cut(s) 283, 674, 731
MflI RGATCY 2 cut(s) 14, 237
MhlI GDGCHC 3 cut(s) 222, 420, 527
MluCI AATT 2 cut(s) 866, 910
MlyI GAGTC 3 cut(s) 263, 274, 322
MmeI TCCRAC 1 cut(s) 717
MnlI CCTC 9 cut(s) 137, 262, 388, 417, 433, 460, 558, 889, 917
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 757
MspI CCGG 2 cut(s) 351, 630
MspR9I CCNGG 3 cut(s) 19, 223, 630
MvaI CCWGG 2 cut(s) 19, 223
MwoI GCNNNNNNNGC 2 cut(s) 360, 557
NciI CCSGG 1 cut(s) 630
NcoI CCATGG 1 cut(s) 156
NlaIII CATG 3 cut(s) 160, 395, 752
NlaIV GGNNCC 4 cut(s) 24, 104, 349, 552
NmuCI GTSAC 3 cut(s) 161, 458, 916
NspV TTCGAA 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 565
PasI CCCWGGG 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 3 cut(s) 55, 452, 506
PkrI GCNGC 4 cut(s) 248, 365, 692, 759
Ple19I CGATCG 1 cut(s) 62
PleI GAGTC 3 cut(s) 262, 274, 321
PpsI GAGTC 3 cut(s) 262, 274, 321
Psp6I CCWGG 2 cut(s) 17, 221
PspEI GGTNACC 1 cut(s) 161
PspGI CCWGG 2 cut(s) 17, 221
PspN4I GGNNCC 4 cut(s) 24, 104, 349, 552
PspPI GGNCC 1 cut(s) 926
PsuI RGATCY 2 cut(s) 14, 237
PvuI CGATCG 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 757
RsaI GTAC 3 cut(s) 488, 519, 697
RsaNI GTAC 3 cut(s) 487, 518, 696
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 4 cut(s) 247, 364, 691, 758
Sau96I GGNCC 1 cut(s) 926
ScaI AGTACT 1 cut(s) 697
SchI GAGTC 3 cut(s) 263, 274, 322
ScrFI CCNGG 3 cut(s) 19, 223, 630
SduI GDGCHC 3 cut(s) 222, 420, 527
SetI ASST 7 cut(s) 85, 104, 559, 728, 759, 816, 828
SfaNI GCATC 1 cut(s) 468
SfcI CTRYAG 1 cut(s) 111
SfuI TTCGAA 1 cut(s) 374
SinI GGWCC 1 cut(s) 926
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 2 cut(s) 866, 910
SsiI CCGC 5 cut(s) 246, 249, 321, 363, 444
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 3 cut(s) 17, 221, 628
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 522, 700
TaqI TCGA 4 cut(s) 58, 129, 374, 834
TasI AATT 2 cut(s) 866, 910
TatI WGTACW 2 cut(s) 517, 695
TauI GCSGC 2 cut(s) 249, 366
TfiI GAWTC 3 cut(s) 55, 452, 506
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 1 cut(s) 374
TseFI GTSAC 3 cut(s) 161, 458, 916
TseI GCWGC 2 cut(s) 690, 757
Tsp45I GTSAC 3 cut(s) 161, 458, 916
TspDTI ATGAA 3 cut(s) 288, 725, 879
TspGWI ACGGA 2 cut(s) 95, 768
TspRI CASTG 1 cut(s) 374
VneI GTGCAC 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 926
XcmI CCANNNNNNNNNTGG 2 cut(s) 154, 398
XspI CTAG 1 cut(s) 543
ZrmI AGTACT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.