Rorug05G0333800

peroxisome matrix targeting signal-2 binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003191
Physical Location & Seq
Reverse (-)
40372038 .. 40375210
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0333800.1

Sequence Viewer

Length: 843 bp
ATGATGATGGTCCTCCATTCTTCGCCGCCTATGGGTGATGGATTCAAACTCCGAGTCCACTCTCACTTTTGTGTGATGTCGAGAGCTGGACTCCTGGCAGGGTATGTGCAATGCAGCCTCGGTTTTGGTTTTCAAGTTTCTTTAATCTTCGATGTGCATAATGGCAGTCAACCTTCATCTCGTAGAAGTTTTGGCTCGAGTACTAGTGATAATGACCGCCTGCGTCAACGGGGTGAACAACCTACATACTCTTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAGAGCCTCGGTTACTGTTTTGAAGTTTGGGCACATGAGCTCAGATCTGCTTGCGTATAGAGCATCAGATGGAACCTTGACAGTATGCACTGTCTCTGATCCTCCTTCAATTCTCAAGCATCTGAATGGTCACTCCAAAGATGTTACAGGTTGGATTGACTGGTATTGTTACCATTATTTTTTCTCTTGCTTTTGCATTGATATATCTGTTTCTAAGTTTCTTGCCTCCACTGATAAGTATATTGTGGATGCATATTTCTTTGGTAAGATTTTGTTTCCTGCATCATCATCTCCACTTGGTTATGGGACCAACGCTATTTTCCCGGTCTTTGTTCTAAACACCATGTCTTGTTTATTTTCAAACTTTGATTTCTCGTCAAACAATCAGTACATTGCATCCTCATCGTTGGATAAAACTGTGAGAGTATGGGAGATTTCAAAAGGCCTATGCATAAGAGTAATATATGGAGTTTCTCCACAACTGTGTATTCGTTTTCACCCTGTAAACGGTAAGGGTTGCATACCTACGCTTGTTGTTTCTTCAGAAAGGAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000268 GO:0001501 GO:0001503 GO:0001764 GO:0001958 GO:0003674 GO:0005048 GO:0005053 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006662 GO:0006810 GO:0006886 GO:0006928 GO:0006996 GO:0007031 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0008152 GO:0008610 GO:0008611 GO:0009056 GO:0009058 GO:0009062 GO:0009653 GO:0009887 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016042 GO:0016043 GO:0016054 GO:0016477 GO:0016558 GO:0016560 GO:0017038 GO:0018904 GO:0019395 GO:0019752 GO:0019899 GO:0022008 GO:0030154 GO:0030258 GO:0031090 GO:0031461 GO:0031903 GO:0031907 GO:0031974 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034440 GO:0034613 GO:0036075 GO:0040011 GO:0042277 GO:0042579 GO:0042802 GO:0042803 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046485 GO:0046504 GO:0046907 GO:0046983 GO:0048513 GO:0048699 GO:0048705 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051674 GO:0055085 GO:0055114 GO:0060348 GO:0060349 GO:0060350 GO:0065002 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0080008 GO:0097384 GO:0098588 GO:0098805 GO:1901503 GO:1901575 GO:1901576 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
WD40

Protein Analysis

280

Amino Acids

31.15

Weight (kDa)

8.7

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 216 - 262 3.3e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 26, 217
AclWI GGATC 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 810
AfaI GTAC 2 cut(s) 202, 674
AfiI CCNNNNNNNGG 2 cut(s) 32, 791
AgsI TTSAA 6 cut(s) 46, 134, 307, 393, 645, 723
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 1 cut(s) 93
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
AleI CACNNNNGTG 2 cut(s) 69, 766
AluBI AGCT 2 cut(s) 86, 324
AluI AGCT 2 cut(s) 86, 324
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 1 cut(s) 727
ApeKI GCWGC 1 cut(s) 114
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspS9I GGNCC 2 cut(s) 10, 591
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 3 cut(s) 47, 245, 773
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 2 cut(s) 10, 591
BaeGI GKGCMC 1 cut(s) 318
BanII GRGCYC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 1 cut(s) 126
BccI CCATC 2 cut(s) 32, 347
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 1 cut(s) 95
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 382
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 1 cut(s) 204
BglII AGATCT 1 cut(s) 328
BisI GCNGC 2 cut(s) 26, 115
BlsI GCNGC 2 cut(s) 27, 116
BmcAI AGTACT 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 95, 608
Bme18I GGWCC 2 cut(s) 10, 591
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 10, 591
BmiI GGNNCC 2 cut(s) 358, 592
BmrFI CCNGG 2 cut(s) 95, 608
BmsI GCATC 6 cut(s) 279, 356, 412, 523, 575, 689
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 608
BsaJI CCNNGG 2 cut(s) 118, 291
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 791
Bse1I ACTGG 1 cut(s) 449
Bse3DI GCAATG 3 cut(s) 116, 265, 675
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 118, 291
BseGI GGATG 2 cut(s) 538, 680
BseLI CCNNNNNNNGG 2 cut(s) 32, 791
BseMI GCAATG 3 cut(s) 116, 265, 675
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 449
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 304
BshFI GGCC 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 608
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 2 cut(s) 32, 791
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 267
BsnI GGCC 1 cut(s) 729
BsoBI CYCGRG 1 cut(s) 196
Bsp1286I GDGCHC 2 cut(s) 318, 326
Bsp143I GATC 3 cut(s) 277, 328, 382
BspACI CCGC 2 cut(s) 26, 217
BspANI GGCC 1 cut(s) 729
BspCNI CTCAG 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 358, 592
BspPI GGATC 1 cut(s) 377
BsrDI GCAATG 3 cut(s) 116, 265, 675
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 118, 291
BssMI GATC 3 cut(s) 277, 328, 382
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 6 cut(s) 301, 367, 376, 703, 768, 794
BstC8I GCNNGC 2 cut(s) 221, 336
BstDEI CTNAG 2 cut(s) 325, 498
BstF5I GGATG 2 cut(s) 538, 680
BstKTI GATC 3 cut(s) 280, 331, 385
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 3 cut(s) 277, 328, 382
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 93, 606
BstSLI GKGCMC 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 1 cut(s) 729
BtsCI GGATG 2 cut(s) 538, 680
BtsIMutI CAGTG 3 cut(s) 287, 372, 513
Cac8I GCNNGC 2 cut(s) 221, 336
Cfr13I GGNCC 2 cut(s) 10, 591
CseI GACGC 1 cut(s) 212
Csp6I GTAC 2 cut(s) 201, 673
CviAII CATG 3 cut(s) 319, 628, 840
CviJI RGCY 6 cut(s) 86, 117, 195, 290, 324, 729
CviKI_1 RGCY 6 cut(s) 86, 117, 195, 290, 324, 729
CviQI GTAC 2 cut(s) 201, 673
DdeI CTNAG 2 cut(s) 325, 498
DpnI GATC 3 cut(s) 279, 330, 384
DpnII GATC 3 cut(s) 277, 328, 382
Ecl136II GAGCTC 1 cut(s) 324
Eco147I AGGCCT 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 326
Eco47I GGWCC 2 cut(s) 10, 591
Eco53kI GAGCTC 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 810
Eco88I CYCGRG 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 93
EcoT22I ATGCAT 3 cut(s) 267, 538, 737
EcoT38I GRGCYC 1 cut(s) 326
FaeI CATG 3 cut(s) 322, 631, 843
FaqI GGGAC 1 cut(s) 604
FatI CATG 3 cut(s) 318, 627, 839
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 2 cut(s) 26, 115
FokI GGATG 2 cut(s) 545, 667
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 26, 115
FspBI CTAG 1 cut(s) 204
GluI GCNGC 2 cut(s) 26, 115
HaeIII GGCC 1 cut(s) 729
HapII CCGG 1 cut(s) 608
HgaI GACGC 1 cut(s) 212
Hin1II CATG 3 cut(s) 322, 631, 843
HincII GTYRAC 2 cut(s) 170, 227
HindII GTYRAC 2 cut(s) 170, 227
HinfI GANTC 3 cut(s) 42, 54, 90
HpaII CCGG 1 cut(s) 608
HphI GGTGA 3 cut(s) 47, 245, 773
Hpy166II GTNNAC 5 cut(s) 58, 170, 227, 236, 790
Hpy188I TCNGA 6 cut(s) 53, 328, 352, 382, 408, 829
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 5 cut(s) 58, 170, 227, 236, 790
HpyAV CCTTC 2 cut(s) 183, 399
HpyCH4III ACNGT 6 cut(s) 301, 367, 376, 703, 768, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 2 cut(s) 325, 498
Hsp92II CATG 3 cut(s) 322, 631, 843
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 277, 328, 382
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 6 cut(s) 279, 356, 412, 523, 575, 689
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 4 cut(s) 295, 413, 427, 452
MalI GATC 3 cut(s) 279, 330, 384
MboI GATC 3 cut(s) 277, 328, 382
MboII GAAGA 3 cut(s) 12, 139, 816
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 318, 326
MluCI AATT 1 cut(s) 393
MlyI GAGTC 2 cut(s) 63, 84
MmeI TCCRAC 2 cut(s) 416, 672
MnlI CCTC 6 cut(s) 23, 128, 301, 396, 520, 694
Mph1103I ATGCAT 3 cut(s) 267, 538, 737
MseI TTAA 1 cut(s) 143
MslI CAYNNNNRTG 3 cut(s) 69, 408, 766
MspI CCGG 1 cut(s) 608
MspR9I CCNGG 2 cut(s) 95, 608
Mva1269I GAATGC 1 cut(s) 267
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 344
NciI CCSGG 1 cut(s) 608
NdeII GATC 3 cut(s) 277, 328, 382
NlaIII CATG 3 cut(s) 322, 631, 843
NlaIV GGNNCC 2 cut(s) 358, 592
NmuCI GTSAC 1 cut(s) 413
NsiI ATGCAT 3 cut(s) 267, 538, 737
OliI CACNNNNGTG 2 cut(s) 69, 766
PaeR7I CTCGAG 1 cut(s) 196
PceI AGGCCT 1 cut(s) 729
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 42
PkrI GCNGC 2 cut(s) 27, 116
PleI GAGTC 2 cut(s) 62, 84
PpsI GAGTC 2 cut(s) 62, 84
Psp124BI GAGCTC 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 358, 592
PspPI GGNCC 2 cut(s) 10, 591
PspXI VCTCGAGB 1 cut(s) 196
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 202, 674
RsaNI GTAC 2 cut(s) 201, 673
RseI CAYNNNNRTG 3 cut(s) 69, 408, 766
SacI GAGCTC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 2 cut(s) 26, 115
Sau3AI GATC 3 cut(s) 277, 328, 382
Sau96I GGNCC 2 cut(s) 10, 591
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 2 cut(s) 63, 84
ScrFI CCNGG 2 cut(s) 95, 608
SduI GDGCHC 2 cut(s) 318, 326
SetI ASST 7 cut(s) 88, 175, 244, 326, 362, 436, 811
SfaNI GCATC 6 cut(s) 279, 356, 412, 523, 575, 689
Sfr274I CTCGAG 1 cut(s) 196
SinI GGWCC 2 cut(s) 10, 591
SlaI CTCGAG 1 cut(s) 196
SmiMI CAYNNNNRTG 3 cut(s) 69, 408, 766
SmlI CTYRAG 2 cut(s) 196, 398
SmoI CTYRAG 2 cut(s) 196, 398
SpeI ACTAGT 1 cut(s) 203
Sse9I AATT 1 cut(s) 393
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 2 cut(s) 26, 217
SspMI CTAG 1 cut(s) 204
SstI GAGCTC 1 cut(s) 326
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 2 cut(s) 93, 606
TaaI ACNGT 6 cut(s) 301, 367, 376, 703, 768, 794
TaqI TCGA 3 cut(s) 80, 150, 197
TasI AATT 1 cut(s) 393
TatI WGTACW 2 cut(s) 200, 672
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 3 cut(s) 287, 379, 520
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 165
TspRI CASTG 3 cut(s) 287, 379, 520
VpaK11BI GGWCC 2 cut(s) 10, 591
XhoI CTCGAG 1 cut(s) 196
XspI CTAG 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 202
Zsp2I ATGCAT 3 cut(s) 267, 538, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.