Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003951

Physical Location & Seq

Genomic Coordinates Reverse (-)

25499508 .. 25503618

Gene Structure 4111 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1164100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 522 bp

ATGGCTACTAGTACTTATTTATTCTTCACTTTGATCATTGCCAGTTCTTTGTGCTCATTCGGAGTTTCTAGGGTGGAACCTCAGAAAGTTACTCTCTCAGTTTACTATGAAACTCTATGTCCCTATTGTGCTACTTTCATCGAAACAAATCTTGCTCGAATTTTCGCCAAGGGTCTCATCACCATTCTCAATCTCAGGCTGGTTCCTTGGGGAAATGCTTCTACAGCCACGTTTTGTCAGCACGGACCAGATGTGTGCAAGCTAAATTCTCTGGAAGCTTGTGCGCTCGATGTTTTACAAGATGTGCTTGAACAAAAATATGCGAATGAAACCACTCACCTCAGTCCGCCTCTTAAGTTCGTTCCATGGCTGGTTCTGAATAATCAACCGATTGGAAATGACTATGAAAATTTCGCTTCCTACGTTTGCAAGGCTTACAAAGGTGCCGCCTTGCCGATGGCCTGCCAATCAGAGTACTCTATCGCCACAGAAATCAATTTCTCAGCTTGCTTAATTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0002376 GO:0002474 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005775 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008285 GO:0009987 GO:0010033 GO:0015036 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0019221 GO:0019882 GO:0019884 GO:0019886 GO:0023052 GO:0030054 GO:0031647 GO:0031974 GO:0034097 GO:0034341 GO:0042127 GO:0042221 GO:0042590 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0047134 GO:0048002 GO:0048145 GO:0048147 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0050896 GO:0051716 GO:0055114 GO:0060333 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071345 GO:0071346

KEGG Pathways

Metabolic & Signaling

ko04612 map04612

Pfam Domains

Protein Families

GILT SapA

Protein Analysis

174

Amino Acids

19.12

Weight (kDa)

5.29

Isoelectric Point (pI)

40.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GILT	PF03227	32 - 102	2.8e-19	Gamma interferon inducible lysosomal thiol reductase (GILT)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0003951)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g03550 FvH4_6g03570 FvH4_6g10810
malus_domestica	MD04G1164100.v1.1 MD12G1177700.v1.1
prunus_persica	Prupe.6G283200_v2.0.a1 Prupe.6G283200_v2.0.a1
pyrus_communis	pycom12g16810
rosa_chinensis	RchiOBHm_Chr3g0461901 RchiOBHm_Chr3g0461911 RchiOBHm_Chr3g0461951 RchiOBHm_Chr3g0462021 RchiOBHm_Chr7g0217871 RchiOBHm_Chr7g0217891 RchiOBHm_Chr7g0217931
rosa_laevigata	RLG00000024885 RLG00000024890
rosa_multiflora	Rmu_sc0005209.1_g000006
rosa_roxburghii	Rroxscaffold_6G00417850
rosa_rugosa	Rorug03G0054500 Rorug07G0172100
rosa_samantha	Rh3AG112500 Rh3AG112700 Rh3CG118200 Rh3DG117300 Rh3DG356700 Rh7AG313300 Rh7AG313700 Rh7BG304300 Rh7BG304400 Rh7DG312200
rosa_wichuraiana	Rw0G007870 Rw0G017260 Rw7G026560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	443
AciI	CCGC	2 cut(s)	347, 447
AcsI	RAATTY	3 cut(s)	159, 265, 409
AfaI	GTAC	2 cut(s)	13, 476
AflII	CTTAAG	1 cut(s)	353
AgsI	TTSAA	1 cut(s)	311
AhlI	ACTAGT	1 cut(s)	8
AluBI	AGCT	3 cut(s)	262, 278, 506
AluI	AGCT	3 cut(s)	262, 278, 506
Alw21I	GWGCWC	1 cut(s)	56
Alw26I	GTCTC	1 cut(s)	179
AoxI	GGCC	1 cut(s)	459
ApoI	RAATTY	3 cut(s)	159, 265, 409
Asp700I	GAANNNNTTC	1 cut(s)	217
AspLEI	GCGC	1 cut(s)	286
AspS9I	GGNCC	1 cut(s)	245
AsuHPI	GGTGA	2 cut(s)	172, 329
AvaII	GGWCC	1 cut(s)	245
BanI	GGYRCC	1 cut(s)	443
Bbv12I	GWGCWC	1 cut(s)	56
BccI	CCATC	1 cut(s)	451
BclI	TGATCA	1 cut(s)	33
BcoDI	GTCTC	1 cut(s)	179
BcuI	ACTAGT	1 cut(s)	8
BfaI	CTAG	2 cut(s)	9, 69
BfmI	CTRYAG	1 cut(s)	222
BfrI	CTTAAG	1 cut(s)	353
BisI	GCNGC	1 cut(s)	447
BlsI	GCNGC	1 cut(s)	448
BmcAI	AGTACT	2 cut(s)	13, 476
Bme18I	GGWCC	1 cut(s)	245
BmgT120I	GGNCC	1 cut(s)	245
BmiI	GGNNCC	3 cut(s)	78, 204, 445
BsaI	GGTCTC	1 cut(s)	179
BsaJI	CCNNGG	3 cut(s)	168, 206, 365
Bse1I	ACTGG	1 cut(s)	42
Bse3DI	GCAATG	1 cut(s)	36
BseDI	CCNNGG	3 cut(s)	168, 206, 365
BseMI	GCAATG	1 cut(s)	36
BseMII	CTCAG	5 cut(s)	95, 111, 208, 355, 516
BseNI	ACTGG	1 cut(s)	42
BshFI	GGCC	1 cut(s)	461
BshNI	GGYRCC	1 cut(s)	443
BsiHKAI	GWGCWC	1 cut(s)	56
BslFI	GGGAC	1 cut(s)	105
BsmAI	GTCTC	1 cut(s)	179
BsmFI	GGGAC	1 cut(s)	105
BsnI	GGCC	1 cut(s)	461
Bso31I	GGTCTC	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	56
Bsp143I	GATC	1 cut(s)	33
Bsp19I	CCATGG	1 cut(s)	365
BspACI	CCGC	2 cut(s)	347, 447
BspANI	GGCC	1 cut(s)	461
BspCNI	CTCAG	5 cut(s)	94, 110, 207, 354, 515
BspLI	GGNNCC	3 cut(s)	78, 204, 445
BspT107I	GGYRCC	1 cut(s)	443
BspTI	CTTAAG	1 cut(s)	353
BspTNI	GGTCTC	1 cut(s)	179
BsrDI	GCAATG	1 cut(s)	36
BsrI	ACTGG	1 cut(s)	42
BssECI	CCNNGG	3 cut(s)	168, 206, 365
BssMI	GATC	1 cut(s)	33
BssT1I	CCWWGG	3 cut(s)	168, 206, 365
BstAFI	CTTAAG	1 cut(s)	353
BstC8I	GCNNGC	3 cut(s)	260, 463, 508
BstDEI	CTNAG	5 cut(s)	81, 97, 194, 341, 502
BstDSI	CCRYGG	1 cut(s)	365
BstHHI	GCGC	1 cut(s)	286
BstKTI	GATC	1 cut(s)	36
BstMAI	GTCTC	1 cut(s)	179
BstMBI	GATC	1 cut(s)	33
BstMWI	GCNNNNNNNGC	1 cut(s)	224
BstSFI	CTRYAG	1 cut(s)	222
BsuRI	GGCC	1 cut(s)	461
BtgI	CCRYGG	1 cut(s)	365
Cac8I	GCNNGC	3 cut(s)	260, 463, 508
CfoI	GCGC	1 cut(s)	286
Cfr13I	GGNCC	1 cut(s)	245
Csp6I	GTAC	2 cut(s)	12, 475
CviAII	CATG	1 cut(s)	366
CviJI	RGCY	9 cut(s)	5, 199, 227, 262, 278, 370, 434, 461, 506
CviKI_1	RGCY	9 cut(s)	5, 199, 227, 262, 278, 370, 434, 461, 506
CviQI	GTAC	2 cut(s)	12, 475
DdeI	CTNAG	5 cut(s)	81, 97, 194, 341, 502
DpnI	GATC	1 cut(s)	35
DpnII	GATC	1 cut(s)	33
EciI	GGCGGA	1 cut(s)	336
Eco130I	CCWWGG	3 cut(s)	168, 206, 365
Eco31I	GGTCTC	1 cut(s)	179
Eco47I	GGWCC	1 cut(s)	245
EcoT14I	CCWWGG	3 cut(s)	168, 206, 365
ErhI	CCWWGG	3 cut(s)	168, 206, 365
FaeI	CATG	1 cut(s)	369
FaiI	YATR	5 cut(s)	108, 118, 321, 367, 405
FalI	AAGNNNNNCTT	2 cut(s)	291, 323
FaqI	GGGAC	1 cut(s)	105
FatI	CATG	1 cut(s)	365
FbaI	TGATCA	1 cut(s)	33
Fnu4HI	GCNGC	1 cut(s)	447
Fsp4HI	GCNGC	1 cut(s)	447
FspBI	CTAG	2 cut(s)	9, 69
GlaI	GCGC	1 cut(s)	285
GluI	GCNGC	1 cut(s)	447
HaeIII	GGCC	1 cut(s)	461
HhaI	GCGC	1 cut(s)	286
Hin1II	CATG	1 cut(s)	369
Hin6I	GCGC	1 cut(s)	284
HinP1I	GCGC	1 cut(s)	284
HindIII	AAGCTT	1 cut(s)	276
HphI	GGTGA	2 cut(s)	172, 329
Hpy166II	GTNNAC	1 cut(s)	103
Hpy188I	TCNGA	4 cut(s)	62, 84, 378, 472
Hpy188III	TCNNGA	1 cut(s)	272
Hpy8I	GTNNAC	1 cut(s)	103
HpyCH4IV	ACGT	2 cut(s)	230, 423
HpyCH4V	TGCA	2 cut(s)	258, 429
HpyF10VI	GCNNNNNNNGC	1 cut(s)	224
HpyF3I	CTNAG	5 cut(s)	81, 97, 194, 341, 502
HpySE526I	ACGT	2 cut(s)	230, 423
Hsp92II	CATG	1 cut(s)	369
HspAI	GCGC	1 cut(s)	284
Ksp22I	TGATCA	1 cut(s)	33
Kzo9I	GATC	1 cut(s)	33
LpnPI	CCDG	7 cut(s)	55, 181, 185, 257, 261, 356, 475
MaeI	CTAG	2 cut(s)	9, 69
MaeII	ACGT	2 cut(s)	230, 423
MaeIII	GTNAC	1 cut(s)	88
MalI	GATC	1 cut(s)	35
MboI	GATC	1 cut(s)	33
MboII	GAAGA	1 cut(s)	16
MhlI	GDGCHC	1 cut(s)	56
MluCI	AATT	5 cut(s)	159, 265, 409, 496, 513
MnlI	CCTC	3 cut(s)	90, 350, 360
MroXI	GAANNNNTTC	1 cut(s)	217
MseI	TTAA	2 cut(s)	354, 512
MspCI	CTTAAG	1 cut(s)	353
MwoI	GCNNNNNNNGC	1 cut(s)	224
NcoI	CCATGG	1 cut(s)	365
NdeII	GATC	1 cut(s)	33
NlaIII	CATG	1 cut(s)	369
NlaIV	GGNNCC	3 cut(s)	78, 204, 445
PcsI	WCGNNNNNNNCGW	1 cut(s)	420
PdmI	GAANNNNTTC	1 cut(s)	217
PkrI	GCNGC	1 cut(s)	448
PspN4I	GGNNCC	3 cut(s)	78, 204, 445
PspPI	GGNCC	1 cut(s)	245
RsaI	GTAC	2 cut(s)	13, 476
RsaNI	GTAC	2 cut(s)	12, 475
SaqAI	TTAA	2 cut(s)	354, 512
SatI	GCNGC	1 cut(s)	447
Sau3AI	GATC	1 cut(s)	33
Sau96I	GGNCC	1 cut(s)	245
ScaI	AGTACT	2 cut(s)	13, 476
SduI	GDGCHC	1 cut(s)	56
SetI	ASST	8 cut(s)	82, 233, 264, 280, 342, 426, 445, 508
SfcI	CTRYAG	1 cut(s)	222
SinI	GGWCC	1 cut(s)	245
SmlI	CTYRAG	1 cut(s)	353
SmoI	CTYRAG	1 cut(s)	353
SpeI	ACTAGT	1 cut(s)	8
Sse9I	AATT	5 cut(s)	159, 265, 409, 496, 513
SsiI	CCGC	2 cut(s)	347, 447
SspMI	CTAG	2 cut(s)	9, 69
StyI	CCWWGG	3 cut(s)	168, 206, 365
TaiI	ACGT	2 cut(s)	233, 426
TaqI	TCGA	3 cut(s)	141, 157, 288
TasI	AATT	5 cut(s)	159, 265, 409, 496, 513
TatI	WGTACW	2 cut(s)	11, 474
TauI	GCSGC	1 cut(s)	449
Tru1I	TTAA	2 cut(s)	354, 512
Tru9I	TTAA	2 cut(s)	354, 512
TspDTI	ATGAA	4 cut(s)	123, 127, 342, 420
TspGWI	ACGGA	1 cut(s)	258
Vha464I	CTTAAG	1 cut(s)	353
VpaK11BI	GGWCC	1 cut(s)	245
XapI	RAATTY	3 cut(s)	159, 265, 409
XmnI	GAANNNNTTC	1 cut(s)	217
XspI	CTAG	2 cut(s)	9, 69
ZrmI	AGTACT	2 cut(s)	13, 476

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.