Rorug03G0054500
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002695
Physical Location & Seq
Forward (+)
4202187 .. 4205755
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0054500.1

Sequence Viewer

Length: 654 bp
ATGAAAGATGGTGCAATACTTAGTATAAATGTACAGTTTTTTCTTAAATTTGGCTCATATATGGAAAAAAGGTTTGGAAGGGCAAGTCAAAGTAATGACCCAGATCAGATAGATCCTGCTAAACCCATATTGAATGTGGCACCATTGAATAGCATGCCATATATTCCCCCAACCACAGAGCCATCAGCAGCCAGAAAGTCCAGTGAACAAGAAAAAGTGAAGCCAGCTATGGTGCTTTTACCTTCACTCGCAACAAGAGAAGAGCGGGATAAGATTTTGGAAACCACCAAAGGGGTTGTATTGACTGGAGTTGCTGCAACGGGACAGGTGGGACCCATAATAGGAAAGGTTGACATTGGTGAAGCTGATGACTCGTACTTATTTCGTGTCTCCCTGCCTGGTGTATCTAGAGATGAGAATTTTAGTTGTTCCATTGAGGCTGATGGAGAAGTGAAAATTAAGGGAACAACTACAACAGGCGAGAAAACAGTGTGCAAGAACTCCATGGTCTTTCAGATGCTCTCACAGAATCTTGCCCCAGCGGGTCACTTCTCTATCTCCTTCCACCTTCCTGGTCCAGTTGATTTCCAGCAGTTCACTGGCTCTTTTGAGACTGATGGGATTCTCGAGGGCATTGTGAAGAAGAAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

217

Amino Acids

23.48

Weight (kDa)

5.79

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 265
AciI CCGC 2 cut(s) 265, 542
AclWI GGATC 1 cut(s) 107
AcsI RAATTY 2 cut(s) 47, 418
AfaI GTAC 2 cut(s) 33, 377
AfiI CCNNNNNNNGG 2 cut(s) 291, 341
AgsI TTSAA 2 cut(s) 133, 148
AjnI CCWGG 2 cut(s) 397, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AluBI AGCT 2 cut(s) 227, 365
AluI AGCT 2 cut(s) 227, 365
Alw26I GTCTC 2 cut(s) 394, 605
AlwI GGATC 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 626
ApeKI GCWGC 2 cut(s) 188, 314
ApoI RAATTY 2 cut(s) 47, 418
AspS9I GGNCC 2 cut(s) 332, 575
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 626
AvaII GGWCC 2 cut(s) 332, 575
BanI GGYRCC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 200, 301
BccI CCATC 4 cut(s) 2, 190, 437, 611
BciT130I CCWGG 2 cut(s) 399, 573
BcoDI GTCTC 2 cut(s) 394, 605
BfaI CTAG 1 cut(s) 408
BisI GCNGC 2 cut(s) 189, 315
BlsI GCNGC 2 cut(s) 190, 316
Bme1390I CCNGG 2 cut(s) 399, 573
Bme18I GGWCC 2 cut(s) 332, 575
BmeT110I CYCGRG 1 cut(s) 626
BmgT120I GGNCC 2 cut(s) 332, 575
BmiI GGNNCC 3 cut(s) 141, 333, 334
BmrFI CCNGG 2 cut(s) 399, 573
BmsI GCATC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 504
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 341
Bse1I ACTGG 4 cut(s) 201, 310, 578, 604
BseBI CCWGG 2 cut(s) 399, 573
BseDI CCNNGG 1 cut(s) 504
BseLI CCNNNNNNNGG 2 cut(s) 291, 341
BseNI ACTGG 4 cut(s) 201, 310, 578, 604
BseXI GCAGC 2 cut(s) 200, 301
BseYI CCCAGC 1 cut(s) 538
BshNI GGYRCC 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 626
BslFI GGGAC 2 cut(s) 336, 345
BslI CCNNNNNNNGG 2 cut(s) 291, 341
BsmAI GTCTC 2 cut(s) 394, 605
BsmFI GGGAC 2 cut(s) 336, 345
BsoBI CYCGRG 1 cut(s) 626
Bsp1407I TGTACA 1 cut(s) 31
Bsp143I GATC 2 cut(s) 103, 112
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 2 cut(s) 265, 542
BspLI GGNNCC 3 cut(s) 141, 333, 334
BspPI GGATC 1 cut(s) 107
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 265
BsrGI TGTACA 1 cut(s) 31
BsrI ACTGG 4 cut(s) 201, 310, 578, 604
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 2 cut(s) 103, 112
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 2 cut(s) 399, 573
Bst4CI ACNGT 2 cut(s) 36, 490
Bst6I CTCTTC 1 cut(s) 255
BstAUI TGTACA 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 155, 225
BstDEI CTNAG 1 cut(s) 20
BstDSI CCRYGG 1 cut(s) 504
BstKTI GATC 2 cut(s) 106, 115
BstMAI GTCTC 2 cut(s) 394, 605
BstMBI GATC 2 cut(s) 103, 112
BstNI CCWGG 2 cut(s) 399, 573
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 397, 571
BstV1I GCAGC 2 cut(s) 200, 301
BstX2I RGATCY 1 cut(s) 112
BstXI CCANNNNNNTGG 1 cut(s) 572
BstYI RGATCY 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 504
BtsIMutI CAGTG 3 cut(s) 208, 495, 597
Cac8I GCNNGC 2 cut(s) 155, 225
Cfr13I GGNCC 2 cut(s) 332, 575
Csp6I GTAC 2 cut(s) 32, 376
CviAII CATG 2 cut(s) 154, 505
CviJI RGCY 8 cut(s) 54, 181, 191, 223, 227, 365, 440, 603
CviKI_1 RGCY 8 cut(s) 54, 181, 191, 223, 227, 365, 440, 603
CviQI GTAC 2 cut(s) 32, 376
DdeI CTNAG 1 cut(s) 20
DpnI GATC 2 cut(s) 105, 114
DpnII GATC 2 cut(s) 103, 112
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco130I CCWWGG 1 cut(s) 504
Eco47I GGWCC 2 cut(s) 332, 575
Eco88I CYCGRG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 397, 571
EcoT14I CCWWGG 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 504
FaeI CATG 2 cut(s) 157, 508
FaqI GGGAC 2 cut(s) 336, 345
FatI CATG 2 cut(s) 153, 504
FauI CCCGC 2 cut(s) 258, 535
Fnu4HI GCNGC 2 cut(s) 189, 315
Fsp4HI GCNGC 2 cut(s) 189, 315
FspBI CTAG 1 cut(s) 408
GluI GCNGC 2 cut(s) 189, 315
GsaI CCCAGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 327
Hin1II CATG 2 cut(s) 157, 508
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 3 cut(s) 371, 529, 622
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 3 cut(s) 206, 352, 597
Hpy188I TCNGA 2 cut(s) 108, 516
Hpy188III TCNNGA 2 cut(s) 408, 626
Hpy8I GTNNAC 3 cut(s) 206, 352, 597
HpyAV CCTTC 4 cut(s) 72, 252, 571, 578
HpyCH4III ACNGT 2 cut(s) 36, 490
HpyCH4V TGCA 3 cut(s) 14, 317, 495
HpyF3I CTNAG 1 cut(s) 20
Hsp92II CATG 2 cut(s) 157, 508
KflI GGGWCCC 1 cut(s) 332
Kzo9I GATC 2 cut(s) 103, 112
LguI GCTCTTC 1 cut(s) 255
Lsp1109I GCAGC 2 cut(s) 200, 301
LweI GCATC 1 cut(s) 507
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 545
MalI GATC 2 cut(s) 105, 114
MbiI CCGCTC 1 cut(s) 265
MboI GATC 2 cut(s) 103, 112
MboII GAAGA 2 cut(s) 272, 652
MflI RGATCY 1 cut(s) 112
MluCI AATT 3 cut(s) 47, 418, 456
MlyI GAGTC 1 cut(s) 365
MnlI CCTC 2 cut(s) 430, 622
MseI TTAA 2 cut(s) 45, 459
MspA1I CMGCKG 1 cut(s) 542
MspR9I CCNGG 2 cut(s) 399, 573
MvaI CCWGG 2 cut(s) 399, 573
NcoI CCATGG 1 cut(s) 504
NdeII GATC 2 cut(s) 103, 112
NlaIII CATG 2 cut(s) 157, 508
NlaIV GGNNCC 3 cut(s) 141, 333, 334
NmuCI GTSAC 1 cut(s) 545
NspI RCATGY 1 cut(s) 157
PaeI GCATGC 1 cut(s) 157
PaeR7I CTCGAG 1 cut(s) 626
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 529, 622
PkrI GCNGC 2 cut(s) 190, 316
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 397, 571
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 2 cut(s) 397, 571
PspN4I GGNNCC 3 cut(s) 141, 333, 334
PspPI GGNCC 2 cut(s) 332, 575
PspPPI RGGWCCY 1 cut(s) 332
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 33, 377
RsaNI GTAC 2 cut(s) 32, 376
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 45, 459
SatI GCNGC 2 cut(s) 189, 315
Sau3AI GATC 2 cut(s) 103, 112
Sau96I GGNCC 2 cut(s) 332, 575
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 2 cut(s) 399, 573
SetI ASST 7 cut(s) 74, 229, 244, 330, 351, 367, 570
SfaNI GCATC 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 626
SinI GGWCC 2 cut(s) 332, 575
SlaI CTCGAG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
SphI GCATGC 1 cut(s) 157
Sse9I AATT 3 cut(s) 47, 418, 456
SsiI CCGC 2 cut(s) 265, 542
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 397, 571
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 36, 490
TaqI TCGA 1 cut(s) 627
TasI AATT 3 cut(s) 47, 418, 456
TatI WGTACW 1 cut(s) 31
TfiI GAWTC 2 cut(s) 529, 622
Tru1I TTAA 2 cut(s) 45, 459
Tru9I TTAA 2 cut(s) 45, 459
TscAI CASTG 3 cut(s) 208, 495, 604
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 2 cut(s) 188, 314
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 208, 495, 604
VpaK11BI GGWCC 2 cut(s) 332, 575
XapI RAATTY 2 cut(s) 47, 418
XbaI TCTAGA 1 cut(s) 407
XceI RCATGY 1 cut(s) 157
XcmI CCANNNNNNNNNTGG 2 cut(s) 133, 596
XhoI CTCGAG 1 cut(s) 626
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.