Rroxscaffold_2G00128620

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002695
Physical Location & Seq
Forward (+)
64386804 .. 64388615
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00128620.1

Sequence Viewer

Length: 519 bp
ATGTTGATCTGGTGCCGGATTCCAAATTCTAACAAGACTAATAAGGCTTCAATGCTGGATGAAGCAATTGAGTATTTGAAGCAGCTCCAGCTTCAAGTACAGTTTTCAGAGGGGAGAAAGGAAGAAACAGAAGAAGAAGGGGGAGACAGGAAGGACAGAAAAGACAGAGAAATCCAAAGGAGGAGAAGAACTGCTATACTTCACATATTCAAGCTGCGAACTTCAGCAGGGAGCTGGCTGACTATTATTTACATCATAGATGTATATGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGGTGATAAACAAGACTGACCTTGCACAAGCAGTTGGAGCTGATTTTGCAGTGATGGAGTGTGATGCATGCACTTCAAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGATAATTTAGCTGCGAACTTCAGTAGGGAGCTGACTGACCATATTATTTACATCATCGATGTATCTGGTGGTGATAAAATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

172

Amino Acids

19.42

Weight (kDa)

6.73

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 81 - 136 3e-08 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 12
AcsI RAATTY 3 cut(s) 25, 279, 510
AcuI CTGAAG 2 cut(s) 207, 436
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 290
AgsI TTSAA 5 cut(s) 51, 79, 95, 211, 399
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 8 cut(s) 85, 91, 214, 234, 314, 362, 443, 463
AluI AGCT 8 cut(s) 85, 91, 214, 234, 314, 362, 443, 463
Alw26I GTCTC 1 cut(s) 138
AoxI GGCC 2 cut(s) 295, 414
ApeKI GCWGC 3 cut(s) 82, 214, 443
ApoI RAATTY 3 cut(s) 25, 279, 510
AspS9I GGNCC 2 cut(s) 296, 414
AsuHPI GGTGA 3 cut(s) 284, 337, 515
BanI GGYRCC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 94, 201, 430
BccI CCATC 2 cut(s) 370, 402
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 138
BisI GCNGC 3 cut(s) 83, 215, 444
BlsI GCNGC 3 cut(s) 84, 216, 445
Bme1390I CCNGG 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 296, 414
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 489
BsaJI CCNNGG 1 cut(s) 298
Bsc4I CCNNNNNNNGG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 300
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 444
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 3 cut(s) 94, 201, 430
BshFI GGCC 2 cut(s) 297, 416
BshNI GGYRCC 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 489
BsiSI CCGG 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 138
BsnI GGCC 2 cut(s) 297, 416
Bsp143I GATC 1 cut(s) 6
BspANI GGCC 2 cut(s) 297, 416
BspCNI CTCAG 1 cut(s) 443
BspDI ATCGAT 1 cut(s) 489
BspLI GGNNCC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 236, 391
BstDEI CTNAG 1 cut(s) 430
BstF5I GGATG 1 cut(s) 64
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 3 cut(s) 88, 359, 368
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 3 cut(s) 94, 201, 430
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 2 cut(s) 297, 416
BsuTUI ATCGAT 1 cut(s) 489
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 2 cut(s) 236, 391
Cfr13I GGNCC 2 cut(s) 296, 414
ClaI ATCGAT 1 cut(s) 489
Csp6I GTAC 1 cut(s) 98
CviAII CATG 2 cut(s) 390, 516
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 1 cut(s) 430
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 207, 436
EcoRII CCWGG 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 2 cut(s) 393, 519
FaiI YATR 8 cut(s) 197, 206, 257, 265, 267, 391, 474, 517
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FatI CATG 2 cut(s) 389, 515
Fnu4HI GCNGC 3 cut(s) 83, 215, 444
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 3 cut(s) 83, 215, 444
GluI GCNGC 3 cut(s) 83, 215, 444
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 297, 416
HapII CCGG 1 cut(s) 16
Hin1II CATG 2 cut(s) 393, 519
HinfI GANTC 2 cut(s) 19, 303
HpaII CCGG 1 cut(s) 16
HphI GGTGA 3 cut(s) 284, 337, 515
Hpy188I TCNGA 2 cut(s) 109, 433
HpyAV CCTTC 3 cut(s) 131, 145, 329
HpyCH4III ACNGT 1 cut(s) 102
HpyCH4V TGCA 4 cut(s) 347, 371, 389, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 359, 368
HpyF3I CTNAG 1 cut(s) 430
Hsp92II CATG 2 cut(s) 393, 519
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 4 cut(s) 90, 231, 359, 460
Lsp1109I GCAGC 3 cut(s) 94, 201, 430
LweI GCATC 1 cut(s) 376
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 4 cut(s) 134, 143, 146, 198
MfeI CAATTG 1 cut(s) 66
MluCI AATT 5 cut(s) 25, 66, 279, 436, 510
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 3 cut(s) 103, 174, 405
Mph1103I ATGCAT 1 cut(s) 391
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 3 cut(s) 88, 359, 368
NdeII GATC 1 cut(s) 6
NlaIII CATG 2 cut(s) 393, 519
NlaIV GGNNCC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 1 cut(s) 393
PaeI GCATGC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 19, 303
PkrI GCNGC 3 cut(s) 84, 216, 445
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 2 cut(s) 296, 414
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SatI GCNGC 3 cut(s) 83, 215, 444
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 2 cut(s) 296, 414
ScrFI CCNGG 1 cut(s) 300
SfaNI GCATC 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SphI GCATGC 1 cut(s) 393
Sse9I AATT 5 cut(s) 25, 66, 279, 436, 510
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 489
TasI AATT 5 cut(s) 25, 66, 279, 436, 510
TatI WGTACW 1 cut(s) 97
TfiI GAWTC 2 cut(s) 19, 303
TscAI CASTG 1 cut(s) 378
TseI GCWGC 3 cut(s) 82, 214, 443
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 1 cut(s) 378
XapI RAATTY 3 cut(s) 25, 279, 510
XceI RCATGY 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.