Rroxscaffold_2G00127760

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002695
Physical Location & Seq
Forward (+)
63361571 .. 63363381
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127760.1

Sequence Viewer

Length: 408 bp
ATGTTGATCTGGTGCCGGATTCCAAATTCTAACAAGACTAATAAGGCTTCAATGCTGGATGAAGCAATTGAGTATTTGAAGCAGCTCCAGCTTCAAGTACAGCTGCGAACTTCAGCAGGGAGCTGGCTGACTATTATTTACATCATAGATGTATATGGTGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGGTGATAAACAAGACTGACCTTGCACAAGCAGTTGGAGCTGATTTTGCAGTGATGGAGTGTGATGCATGCACTTCAAATGCGAGATGGAGGGCCATTTGTCTTTGCTCAGATAATTTAGCTGCGAACTTCAGTAGGGAGCTGACTGACCATATTATTTACATCATCGATGTATCTGGTGGTGATAAAATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

135

Amino Acids

14.86

Weight (kDa)

5.27

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 43 - 99 9.5e-09 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 12
AcsI RAATTY 3 cut(s) 25, 168, 399
AcuI CTGAAG 2 cut(s) 96, 325
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 4 cut(s) 51, 79, 95, 288
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 8 cut(s) 85, 91, 103, 123, 203, 251, 332, 352
AluI AGCT 8 cut(s) 85, 91, 103, 123, 203, 251, 332, 352
AoxI GGCC 2 cut(s) 184, 303
ApeKI GCWGC 3 cut(s) 82, 103, 332
ApoI RAATTY 3 cut(s) 25, 168, 399
AspS9I GGNCC 2 cut(s) 185, 303
AsuHPI GGTGA 3 cut(s) 173, 226, 404
BanI GGYRCC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 90, 94, 319
BccI CCATC 2 cut(s) 259, 291
BciT130I CCWGG 1 cut(s) 189
BisI GCNGC 3 cut(s) 83, 104, 333
BlsI GCNGC 3 cut(s) 84, 105, 334
Bme1390I CCNGG 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 185, 303
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 183
Bsa29I ATCGAT 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 189
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 333
BseXI GCAGC 3 cut(s) 90, 94, 319
BshFI GGCC 2 cut(s) 186, 305
BshNI GGYRCC 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 378
BsiSI CCGG 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 179
BsnI GGCC 2 cut(s) 186, 305
Bsp143I GATC 1 cut(s) 6
BspANI GGCC 2 cut(s) 186, 305
BspCNI CTCAG 1 cut(s) 332
BspDI ATCGAT 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 125, 280
BstDEI CTNAG 1 cut(s) 319
BstF5I GGATG 1 cut(s) 64
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 3 cut(s) 88, 248, 257
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 90, 94, 319
Bsu15I ATCGAT 1 cut(s) 378
BsuRI GGCC 2 cut(s) 186, 305
BsuTUI ATCGAT 1 cut(s) 378
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 1 cut(s) 267
Cac8I GCNNGC 2 cut(s) 125, 280
Cfr13I GGNCC 2 cut(s) 185, 303
ClaI ATCGAT 1 cut(s) 378
Csp6I GTAC 1 cut(s) 98
CviAII CATG 2 cut(s) 279, 405
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 1 cut(s) 319
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 96, 325
EcoRII CCWGG 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 280
FaeI CATG 2 cut(s) 282, 408
FaiI YATR 6 cut(s) 146, 154, 156, 280, 363, 406
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FatI CATG 2 cut(s) 278, 404
Fnu4HI GCNGC 3 cut(s) 83, 104, 333
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 3 cut(s) 83, 104, 333
GluI GCNGC 3 cut(s) 83, 104, 333
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 186, 305
HapII CCGG 1 cut(s) 16
Hin1II CATG 2 cut(s) 282, 408
HinfI GANTC 2 cut(s) 19, 192
HpaII CCGG 1 cut(s) 16
HphI GGTGA 3 cut(s) 173, 226, 404
Hpy188I TCNGA 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 218
HpyCH4V TGCA 4 cut(s) 236, 260, 278, 282
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 248, 257
HpyF3I CTNAG 1 cut(s) 319
Hsp92II CATG 2 cut(s) 282, 408
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 4 cut(s) 90, 120, 248, 349
LpnPI CCDG 9 cut(s) 29, 41, 101, 102, 109, 174, 197, 201, 372
Lsp1109I GCAGC 3 cut(s) 90, 94, 319
LweI GCATC 1 cut(s) 265
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MfeI CAATTG 1 cut(s) 66
MluCI AATT 5 cut(s) 25, 66, 168, 325, 399
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 1 cut(s) 294
Mph1103I ATGCAT 1 cut(s) 280
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 88, 248, 257
NdeII GATC 1 cut(s) 6
NlaIII CATG 2 cut(s) 282, 408
NlaIV GGNNCC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 1 cut(s) 282
PaeI GCATGC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 19, 192
PkrI GCNGC 3 cut(s) 84, 105, 334
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 2 cut(s) 185, 303
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SatI GCNGC 3 cut(s) 83, 104, 333
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 2 cut(s) 185, 303
ScrFI CCNGG 1 cut(s) 189
SfaNI GCATC 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 282
Sse9I AATT 5 cut(s) 25, 66, 168, 325, 399
StyD4I CCNGG 1 cut(s) 187
TaqI TCGA 1 cut(s) 378
TasI AATT 5 cut(s) 25, 66, 168, 325, 399
TatI WGTACW 1 cut(s) 97
TfiI GAWTC 2 cut(s) 19, 192
TscAI CASTG 1 cut(s) 267
TseI GCWGC 3 cut(s) 82, 103, 332
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 1 cut(s) 267
XapI RAATTY 3 cut(s) 25, 168, 399
XceI RCATGY 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.